LOCUS       NM_001079875            2113 bp    mRNA    linear   ROD 04-APR-2024
DEFINITION  Mus musculus sperm adhesion molecule 1 (Spam1), transcript variant
            1, mRNA.
ACCESSION   NM_001079875
VERSION     NM_001079875.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2113)
  AUTHORS   Park,S., Kim,Y.H., Jeong,P.S., Park,C., Lee,J.W., Kim,J.S., Wee,G.,
            Song,B.S., Park,B.J., Kim,S.H., Sim,B.W., Kim,S.U.,
            Triggs-Raine,B., Baba,T., Lee,S.R. and Kim,E.
  TITLE     SPAM1/HYAL5 double deficiency in male mice leads to severe male
            subfertility caused by a cumulus-oocyte complex penetration defect
  JOURNAL   FASEB J 33 (12), 14440-14449 (2019)
   PUBMED   31670981
  REMARK    GeneRIF: A comparative analysis between WT and Spam1/Hyal5 dKO
            epididymal sperm revealed that the absence of these 2 sperm Hyals
            resulted in a marked accumulation of sperm on the outside of the
            cumulus-oocyte complex.
REFERENCE   2  (bases 1 to 2113)
  AUTHORS   Preston,M., Gong,X., Su,W., Matsumoto,S.G., Banine,F., Winkler,C.,
            Foster,S., Xing,R., Struve,J., Dean,J., Baggenstoss,B.,
            Weigel,P.H., Montine,T.J., Back,S.A. and Sherman,L.S.
  TITLE     Digestion products of the PH20 hyaluronidase inhibit remyelination
  JOURNAL   Ann Neurol 73 (2), 266-280 (2013)
   PUBMED   23463525
  REMARK    GeneRIF: PH20 is elevated in demyelinating lesions and that
            increased PH20 expression is sufficient to inhibit oligodendrocyte
            precursor cell maturation and remyelination.
REFERENCE   3  (bases 1 to 2113)
  AUTHORS   Zhou,C., Kang,W. and Baba,T.
  TITLE     Functional characterization of double-knockout mouse sperm lacking
            SPAM1 and ACR or SPAM1 and PRSS21 in fertilization
  JOURNAL   J Reprod Dev 58 (3), 330-337 (2012)
   PUBMED   22362218
  REMARK    GeneRIF: ACR or PRSS21 or both may function cooperatively with
            SPAM1 in sperm/cumulus penetration
REFERENCE   4  (bases 1 to 2113)
  AUTHORS   Reese,K.L., Aravindan,R.G., Griffiths,G.S., Shao,M., Wang,Y.,
            Galileo,D.S., Atmuri,V., Triggs-Raine,B.L. and Martin-Deleon,P.A.
  TITLE     Acidic hyaluronidase activity is present in mouse sperm and is
            reduced in the absence of SPAM1: evidence for a role for
            hyaluronidase 3 in mouse and human sperm
  JOURNAL   Mol Reprod Dev 77 (9), 759-772 (2010)
   PUBMED   20586096
REFERENCE   5  (bases 1 to 2113)
  AUTHORS   Kang,W., Zhou,C., Koga,Y. and Baba,T.
  TITLE     Hyaluronan-degrading activity of mouse sperm hyaluronidase is not
            required for fertilization?
  JOURNAL   J Reprod Dev 56 (1), 140-144 (2010)
   PUBMED   19926939
  REMARK    GeneRIF: The data from this paper suggests that the
            hyaluronan-degrading activity of the sperm hyaluronidase SPAM1 may
            not be required for fertilization, at least in the mouse.
REFERENCE   6  (bases 1 to 2113)
  AUTHORS   Baba,D., Kashiwabara,S., Honda,A., Yamagata,K., Wu,Q., Ikawa,M.,
            Okabe,M. and Baba,T.
  TITLE     Mouse sperm lacking cell surface hyaluronidase PH-20 can pass
            through the layer of cumulus cells and fertilize the egg
  JOURNAL   J Biol Chem 277 (33), 30310-30314 (2002)
   PUBMED   12065596
  REMARK    GeneRIF: sperm lacking cell surface hyaluronidase PH-20 can pass
            through the layer of cumulus cells and fertilize the egg
REFERENCE   7  (bases 1 to 2113)
  AUTHORS   Zheng,Y., Deng,X., Zhao,Y., Zhang,H. and Martin-DeLeon,P.A.
  TITLE     Spam1 (PH-20) mutations and sperm dysfunction in mice with the
            Rb(6.16) or Rb(6.15) translocation
  JOURNAL   Mamm Genome 12 (11), 822-829 (2001)
   PUBMED   11845284
  REMARK    GeneRIF: Entrapment of spontaneous Spam1 mutations, owing to
            recombination suppression near the Robertsonion translocation
            junctions, is proposed as the major underlying defect of the sperm
            dysfunction.
REFERENCE   8  (bases 1 to 2113)
  AUTHORS   Deng,X., Czymmek,K. and Martin-DeLeon,P.A.
  TITLE     Biochemical maturation of Spam1 (PH-20) during epididymal transit
            of mouse sperm involves modifications of N-linked oligosaccharides
  JOURNAL   Mol Reprod Dev 52 (2), 196-206 (1999)
   PUBMED   9890751
REFERENCE   9  (bases 1 to 2113)
  AUTHORS   Deng,X., Moran,J., Copeland,N.G., Gilbert,D.J., Jenkins,N.A.,
            Primakoff,P. and Martin-DeLeon,P.A.
  TITLE     The mouse Spam1 maps to proximal chromosome 6 and is a candidate
            for the sperm dysfunction in Rb(6.16)24Lub and Rb(6.15)1Ald
            heterozygotes
  JOURNAL   Mamm Genome 8 (2), 94-97 (1997)
   PUBMED   9060406
REFERENCE   10 (bases 1 to 2113)
  AUTHORS   Thaler,C.D. and Cardullo,R.A.
  TITLE     Biochemical characterization of a
            glycosylphosphatidylinositol-linked hyaluronidase on mouse sperm
  JOURNAL   Biochemistry 34 (24), 7788-7795 (1995)
   PUBMED   7794889
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AY920282.1 and AK005638.1.
            
            On Nov 7, 2013 this sequence version replaced NM_001079875.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript. Variants 1 and 2 encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK005638.1, AY920283.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AY920282.1         1-13
            14-1811             AK005638.1         1-1798
            1812-2113           AY920282.1         1806-2107
FEATURES             Location/Qualifiers
     source          1..2113
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 11.28 cM"
     gene            1..2113
                     /gene="Spam1"
                     /gene_synonym="Ph-20"
                     /note="sperm adhesion molecule 1"
                     /db_xref="GeneID:20690"
                     /db_xref="MGI:MGI:109335"
     exon            1..95
                     /gene="Spam1"
                     /gene_synonym="Ph-20"
                     /inference="alignment:Splign:2.1.0"
     exon            96..130
                     /gene="Spam1"
                     /gene_synonym="Ph-20"
                     /inference="alignment:Splign:2.1.0"
     exon            131..1282
                     /gene="Spam1"
                     /gene_synonym="Ph-20"
                     /inference="alignment:Splign:2.1.0"
     CDS             329..1867
                     /gene="Spam1"
                     /gene_synonym="Ph-20"
                     /EC_number="3.2.1.35"
                     /note="hyaluronidase PH-20; hyal-PH20; sperm surface
                     protein PH-20; hyaluronoglucosaminidase PH-20"
                     /codon_start=1
                     /product="hyaluronidase PH-20 precursor"
                     /protein_id="NP_001073344.1"
                     /db_xref="CCDS:CCDS19947.1"
                     /db_xref="GeneID:20690"
                     /db_xref="MGI:MGI:109335"
                     /translation="MGELRFKHLFWGSFVESGGTFQTVLIFLLIPCSLTVDYRAAPIL
                     SNTTFLWIWNVPTERCVGNVNDPIDLSFFSLIGSPRKTATGQPVTLFYVDRLGLYPHI
                     DANQAEHYGGIPQRGDYQAHLRKAKTDIEHYIPDDKLGLAIIDWEEWRPTWLRNWKPK
                     DNYRNKSIELVQSTNPGLSITEATQKAIQQFEEAGRKFMEGTLHLGKFLRPNQLWGYY
                     LFPDCYNNKFQDPKYDGQCPAVEKKRNDNLKWLWKASTGLYPSVYLKKDLKSNRQATL
                     YVRYRVVEAIRVSKVGNASDPVPIFVYIRLVFTDRTSEYLLEDDLVNTIGEIVALGTS
                     GIIIWDAMSLAQRAAGCPILHKYMQTTLNPYIVNVTLAAKMCSQTLCNEKGMCSRRKE
                     SSDVYLHLNPSHFDIMLTETGKYEVLGNPRVGDLEYFSEHFKCSCFSRMTCKETSDVK
                     NVQDVNVCVGDNVCIKAKVEPNPAFYLLPGKSLLFMTTLGHVLYHLPQDIFVFPRKTL
                     VSTP"
     exon            1283..1372
                     /gene="Spam1"
                     /gene_synonym="Ph-20"
                     /inference="alignment:Splign:2.1.0"
     exon            1373..2113
                     /gene="Spam1"
                     /gene_synonym="Ph-20"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 cttggcttat tctaagagtg ctgaagtaga tttagattga ccatggctca catgaattaa
       61 gaagtgtttc ttttgttatg atggagatgc agatggtgaa agtgatgaaa gaattgtcac
      121 tcagtggaag ttctggatga tttaacttgc tccaagatat tcctgaaatg taacacagga
      181 agaagaatct tcagtgtaaa tcagtcacca tacattcatc tccctcagta gcctcatgcc
      241 acagtctttc taatcttttg catcaaatac tggacagacc acagtgtgta agaaggaata
      301 agtgcctact tagtaattat tctctgtgat gggagagttg agatttaagc acctcttttg
      361 ggggagcttt gttgaatctg ggggcacatt ccaaacagtg ttaatcttcc ttttgattcc
      421 atgctccttg actgtggatt atagggcagc accaatttta tcaaatacaa ctttcctttg
      481 gatttggaat gtcccaactg aacgttgtgt aggaaatgtt aatgatccaa tagatctgag
      541 cttcttctct ttaattggaa gcccccggaa aactgccaca gggcaacctg tcacattatt
      601 ttatgttgat cgacttggtt tgtatcctca catagatgca aaccaagcag aacattatgg
      661 aggaatacct cagaggggcg attatcaagc tcatttgcgc aaagctaaga ctgacataga
      721 gcattacatt ccagacgaca aattgggctt agctatcatt gactgggaag aatggaggcc
      781 tacctggttg agaaactgga aacctaagga taactacagg aataagtcta ttgaattggt
      841 ccaatcaact aatccaggac ttagtatcac agaagccacc cagaaagcca tacaacaatt
      901 tgaagaggca ggaaggaagt ttatggaagg aactttacac ctggggaaat tccttcgacc
      961 aaaccagcta tggggttatt atctatttcc tgattgttat aacaataagt ttcaagaccc
     1021 taagtatgat gggcagtgcc ctgctgtgga aaagaaaaga aatgataatc ttaaatggct
     1081 atggaaagca agcaccggcc tttacccatc tgtctatttg aagaaagact tgaagtccaa
     1141 tcgacaagct accctctatg tccgctaccg agttgtggaa gctatcagag tgtccaaggt
     1201 tgggaatgca tcggatccag tcccgatttt tgtctatatc cgtcttgttt ttactgatcg
     1261 tacctctgaa taccttctag aggatgacct tgtgaataca attggtgaaa ttgttgctct
     1321 gggtacctct ggaattataa tatgggatgc tatgagttta gcacaacgtg cggcaggttg
     1381 cccaatccta cataaataca tgcagacgac cctgaatcca tacatagtca atgttaccct
     1441 agcagccaaa atgtgcagcc aaacactttg taatgagaaa ggcatgtgtt caagaagaaa
     1501 agaaagttca gatgtatatc ttcacttgaa cccaagtcat tttgatatta tgttaacgga
     1561 aactggaaag tacgaagttc ttggcaaccc cagggttgga gacttagaat acttttctga
     1621 acattttaaa tgcagctgtt ttagcagaat gacatgtaag gagacatctg atgtaaaaaa
     1681 tgtacaagac gtgaatgtgt gcgtcggtga caatgtttgt ataaaagcca aggtagaacc
     1741 caacccagcc ttctacctcc tacctggcaa aagccttcta tttatgacaa cacttggtca
     1801 tgtgctgtac catctgccac aagatatttt tgtttttcca cggaagacac tagtcagtac
     1861 tccttagttt tctctaccca cagcgtttga tgtattatta ttattatttt tgcaggcctc
     1921 agtaatttgg gattatgaat gggattctat tttaccaaag taattcaatt tttataatca
     1981 agattctatt tttgagtttc aaagagaaat tatatattct tctaccaaag attgattaca
     2041 agcaaggcta cttagggatt agttttggtt taaagagaat gaagactgaa taaaataaaa
     2101 tcactagaaa att
//
