LOCUS       NM_001290462            1596 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus sperm associated antigen 8 (Spag8), transcript variant
            1, mRNA.
ACCESSION   NM_001290462 XM_006538053
VERSION     NM_001290462.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1596)
  AUTHORS   Tai,L., Yin,G., Huang,X., Sun,F. and Zhu,Y.
  TITLE     In-cell structural insight into the stability of sperm microtubule
            doublet
  JOURNAL   Cell Discov 9 (1), 116 (2023)
   PUBMED   37989994
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1596)
  AUTHORS   Chen,Z., Shiozaki,M., Haas,K.M., Skinner,W.M., Zhao,S., Guo,C.,
            Polacco,B.J., Yu,Z., Krogan,N.J., Lishko,P.V., Kaake,R.M.,
            Vale,R.D. and Agard,D.A.
  TITLE     De novo protein identification in mammalian sperm using in situ
            cryoelectron tomography and AlphaFold2 docking
  JOURNAL   Cell 186 (23), 5041-5053 (2023)
   PUBMED   37865089
REFERENCE   3  (bases 1 to 1596)
  AUTHORS   Zhou,L., Liu,H., Liu,S., Yang,X., Dong,Y., Pan,Y., Xiao,Z.,
            Zheng,B., Sun,Y., Huang,P., Zhang,X., Hu,J., Sun,R., Feng,S.,
            Zhu,Y., Liu,M., Gui,M. and Wu,J.
  TITLE     Structures of sperm flagellar doublet microtubules expand the
            genetic spectrum of male infertility
  JOURNAL   Cell 186 (13), 2897-2910 (2023)
   PUBMED   37295417
REFERENCE   4  (bases 1 to 1596)
  AUTHORS   Wu,H., Chen,Y., Miao,S., Zhang,C., Zong,S., Koide,S.S. and Wang,L.
  TITLE     Sperm associated antigen 8 (SPAG8), a novel regulator of activator
            of CREM in testis during spermatogenesis
  JOURNAL   FEBS Lett 584 (13), 2807-2815 (2010)
   PUBMED   20488182
  REMARK    GeneRIF: Results indicate that SPAG8 acts as a regulator of ACT and
            plays an important role in CREM-ACT-mediated gene transcription
            during spermatogenesis.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732626.8 and AK132968.1.
            
            On Nov 26, 2015 this sequence version replaced NM_001290462.1.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK132968.1, SRR5189670.78077.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-42                AL732626.8         91122-91163         c
            43-1596             AK132968.1         2-1555
FEATURES             Location/Qualifiers
     source          1..1596
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 23.05 cM"
     gene            1..1596
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /note="sperm associated antigen 8"
                     /db_xref="GeneID:433700"
                     /db_xref="MGI:MGI:3056295"
     exon            1..91
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            92..872
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            873..1047
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1093
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1094..1208
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1271
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /inference="alignment:Splign:2.1.0"
     exon            1272..1596
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1570..1575
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /note="hexamer: CATAAA"
     polyA_site      1591
                     /gene="Spag8"
                     /gene_synonym="MH-SPAG8; SMP-1"
                     /note="major polyA site"
ORIGIN      
        1 tctcctggag ttgtttgccg tctctatggc aacccgggag gtggagtccg aaaatggaga
       61 ccaccgagtc tacagagggg tctttgtcgc gatcttgtga tgtacagccc agctccgaaa
      121 gactagatac cccttcagaa cctgttcctt cttcgagtag tagtcctagg tctactgccc
      181 ctgccgaggc tccggcgcag tactctgtgc tgacagagcc ctcttctgac agtctttacg
      241 gggcaccctg ccctccagcc catcatagag gccatgggtt tggctttcag cccttctatg
      301 tttcttgtat tcctcaggat ccctgtaata tggcagacct cagctccagg gctgatccta
      361 cctcttctta tccttgtcat agctctgtgc atggctctgg ctctggcact tgtggtcttg
      421 gccagagctc tgagcctagc cagggctccg ggcccacctc tgggcctgct cctgcctctg
      481 tacctagtct tgtctctgga cctgattctg cctctggacc tgattcttct gcctcgggac
      541 ctgctcttgc ctctggacct ggtcctgctg accctggaca gggtcctaag ttcagcacct
      601 gcattcctca agggtacaga tgcatcccag ttgacctggc ccctgattat aatgcatggt
      661 gtcagcactt acactggaag ccacaacgct cctgggaacc tctgcaagtc tcagaacctg
      721 gagtgagagg gccatataaa cctccagaac ctggagcact cgggccatgt gagccatgtg
      781 agccatgtga acctccagag gctgaatctg aggaaactct ctgtaaagca cggccacgtg
      841 gccagtgcct cctctacaac tgggaggaag agagagccac caaccagctg gatcaaatcc
      901 ctccattgca agatggctct gagagttact tcttccgaca cggacaccag ggtctgctga
      961 ccacgcagcc ccagtcaccc atgtcttcta gcaccaccca gagagactca taccagctcc
     1021 caagacacat ctgtcagcca cttcgaggga agcgtgaagc catgctggag atgctcctgc
     1081 gacaccagat ctgtaaagag gtgcaggcag agcaggaacc tgcaaggaag ctctttgaga
     1141 ccgagtcggt aacacaccat gactacagag tggagctggt acgagcagcg cctcctgcct
     1201 caacaaagcc tcatgattac cgccaggagc agcctgaaac cttctggatt cagcgggcag
     1261 cacggctacc gggtgtcagt gacatcagga cactggacac accattccgg aagaactgca
     1321 gcttctcgac gcccgtgccc ttgtcgctgg ggcagccctt gccttacgag cttgagagcg
     1381 gcccccacca agtgggggta atatcttccc ttgcctgtca gggaggaggg caggggtgtg
     1441 gaaggacgaa aacaactcca atctaagaat cgaggagaga agcagacgtg ggatttgaaa
     1501 tcagtttctg tttatactac agaggtagat tggatggagt tgattctctg tccccgatga
     1561 ggggttttac ataaagcact ctcatcctca agctgc
//
