LOCUS       NM_015773               2483 bp    mRNA    linear   ROD 04-AUG-2023
DEFINITION  Mus musculus sperm associated antigen 6-like (Spag6l), mRNA.
ACCESSION   NM_015773
VERSION     NM_015773.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2483)
  AUTHORS   Li X, Zhang D, Xu L, Liu W, Zhang N, Strauss JF 3rd, Zhang Z and
            Wang H.
  TITLE     Sperm-associated antigen 6 (Spag6) mutation leads to vestibular
            dysfunction in mice
  JOURNAL   J Pharmacol Sci 147 (4), 325-330 (2021)
   PUBMED   34663514
REFERENCE   2  (bases 1 to 2483)
  AUTHORS   Li X, Zhang D, Xu L, Han Y, Liu W, Li W, Fan Z, Costanzo RM,
            Strauss Iii JF, Zhang Z and Wang H.
  TITLE     Planar cell polarity defects and hearing loss in sperm-associated
            antigen 6 (Spag6)-deficient mice
  JOURNAL   Am J Physiol Cell Physiol 320 (1), C132-C141 (2021)
   PUBMED   33175573
REFERENCE   3  (bases 1 to 2483)
  AUTHORS   Miyata H, Morohoshi A and Ikawa M.
  TITLE     Analysis of the sperm flagellar axoneme using gene-modified mice
  JOURNAL   Exp Anim 69 (4), 374-381 (2020)
   PUBMED   32554934
  REMARK    Review article
REFERENCE   4  (bases 1 to 2483)
  AUTHORS   Liu Y, Zhang L, Li W, Huang Q, Yuan S, Li Y, Liu J, Zhang S, Pin G,
            Song S, Ray PF, Arnoult C, Cho C, Garcia-Reyes B, Knippschild U,
            Strauss JF and Zhang Z.
  TITLE     The sperm-associated antigen 6 interactome and its role in
            spermatogenesis
  JOURNAL   Reproduction 158 (2), 181-197 (2019)
   PUBMED   31146259
REFERENCE   5  (bases 1 to 2483)
  AUTHORS   Zheng J, Liu H, Zhu L, Chen Y, Zhao H, Zhang W, Li F, Xie L, Yan X
            and Zhu X.
  TITLE     Microtubule-bundling protein Spef1 enables mammalian ciliary
            central apparatus formation
  JOURNAL   J Mol Cell Biol 11 (1), 67-77 (2019)
   PUBMED   30535028
REFERENCE   6  (bases 1 to 2483)
  AUTHORS   Qiu H, Golas A, Grzmil P and Wojnowski L.
  TITLE     Lineage-specific duplications of Muroidea Faim and Spag6 genes and
            atypical accelerated evolution of the parental Spag6 gene
  JOURNAL   J Mol Evol 77 (3), 119-129 (2013)
   PUBMED   24071998
REFERENCE   7  (bases 1 to 2483)
  AUTHORS   Zhang Z, Jones BH, Tang W, Moss SB, Wei Z, Ho C, Pollack M,
            Horowitz E, Bennett J, Baker ME and Strauss JF 3rd.
  TITLE     Dissecting the axoneme interactome: the mammalian orthologue of
            Chlamydomonas PF6 interacts with sperm-associated antigen 6, the
            mammalian orthologue of Chlamydomonas PF16
  JOURNAL   Mol Cell Proteomics 4 (7), 914-923 (2005)
   PUBMED   15827353
REFERENCE   8  (bases 1 to 2483)
  AUTHORS   Zhang Z, Sapiro R, Kapfhamer D, Bucan M, Bray J, Chennathukuzhi V,
            McNamara P, Curtis A, Zhang M, Blanchette-Mackie EJ and Strauss JF
            3rd.
  TITLE     A sperm-associated WD repeat protein orthologous to Chlamydomonas
            PF20 associates with Spag6, the mammalian orthologue of
            Chlamydomonas PF16
  JOURNAL   Mol Cell Biol 22 (22), 7993-8004 (2002)
   PUBMED   12391165
  REMARK    GeneRIF: Data describe the cloning of human and mouse Pf20 proteins
            that interact with Spag6 protein.
REFERENCE   9  (bases 1 to 2483)
  AUTHORS   Sapiro R, Kostetskii I, Olds-Clarke P, Gerton GL, Radice GL and
            Strauss III JF.
  TITLE     Male infertility, impaired sperm motility, and hydrocephalus in
            mice deficient in sperm-associated antigen 6
  JOURNAL   Mol Cell Biol 22 (17), 6298-6305 (2002)
   PUBMED   12167721
  REMARK    GeneRIF: Spag6 is essential for sperm flagellar motility and that
            it is important for the maintenance of the structural integrity of
            mature sperm. The occurrence of hydrocephalus in the mutant mice
            also implicates Spag6 in the motility of ependymal cilia.
REFERENCE   10 (bases 1 to 2483)
  AUTHORS   Sapiro R, Tarantino LM, Velazquez F, Kiriakidou M, Hecht NB, Bucan
            M and Strauss JF 3rd.
  TITLE     Sperm antigen 6 is the murine homologue of the Chlamydomonas
            reinhardtii central apparatus protein encoded by the PF16 locus
  JOURNAL   Biol Reprod 62 (3), 511-518 (2000)
   PUBMED   10684790
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ060006.1 and BC090660.1.
            
            [WARNING] On Jan 18, 2024 this sequence was replaced by
            NM_015773.3.
            
            On Apr 14, 2009 this sequence version replaced NM_015773.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC090660.1, AF173866.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-57                CJ060006.1         1-57
            58-2483             BC090660.1         1-2426
FEATURES             Location/Qualifiers
     source          1..2483
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="16"
                     /map="16 10.39 cM"
     gene            1..2483
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /note="sperm associated antigen 6-like"
                     /db_xref="GeneID:50525"
                     /db_xref="MGI:MGI:1354388"
     exon            1..169
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..1668
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /note="axoneme protein; protein PF16 homolog; axoneme
                     central apparatus protein"
                     /codon_start=1
                     /product="sperm-associated antigen 6"
                     /protein_id="NP_056588.1"
                     /db_xref="CCDS:CCDS27988.1"
                     /db_xref="GeneID:50525"
                     /db_xref="MGI:MGI:1354388"
                     /translation="MSQRQVLQVFEQYQKARTQFVQMVAELATRPQNIETLQNAGVMS
                     LLRPLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNRFYKKA
                     AAFVLRAVGKHSPQLAQAIVDCGALDTLVICLEDFDPGVKEAAAWALGYIARHNTELS
                     QAVVDAGAIPLLVLCIQEPEIALKRIAASALSDISKHSPELAQTVVDAGAIAHLAQMI
                     LNPDAKLKRQVLSALSQIAKHSVDLAEMVVEAEIFPVVLTCLKDKDEYVKKNACTLIR
                     EIAKHTPELSQLIVNAGGVAAVIDCIGSCKGNIRLPGIMMLGYVAAHSENLAMAVIIS
                     KGVPQLSICLSEEPEDHIKAAAAWALGQLGRHTPEHARAVAVTNTLPVLLSLYMSPES
                     SEDLQLKSKKAIKNILQKCTYLPALEPFLYDAPPNILKHVVGQFSKVLPHDSKARRLF
                     VTSGGLKKVQEIKAEPGSLLQEYINSINNCYPEEIVRYYSPGYSDTLLQRVDSYQPL"
     exon            170..265
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     exon            266..432
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     exon            433..616
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     exon            617..822
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     exon            823..996
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1149
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1150..1341
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1342..1458
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1604
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..2466
                     /gene="Spag6l"
                     /gene_synonym="PF16; Spag6; Spag6l1"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 gacctttgct ggtggttgta tccggttgct gtcggttgcc ctggagacgc atacacagag
       61 gaacggatcc cactaccagc tcaacggtgg cggaggtcct ggtagctgcc caccacttgg
      121 acctggagaa gcaggtgcgg cgccatgagc cagcggcagg tgctgcaagt gttcgagcag
      181 taccagaaag cccggacgca attcgtgcag atggtggcag agctggcgac aagaccccaa
      241 aacatcgaaa cgctgcagaa tgcaggtgta atgtctttgc taaggccgct tcttctggat
      301 gtggtcccaa caattcaaca gactgcagct ttggctctgg ggagactggc caattacaat
      361 gatgacttag cagaagcagt cgtgaaaggt gacattcttc cacagcttgt ttattcattg
      421 gcagaacaaa atcgttttta caagaaggca gctgcctttg tgttgagagc agttgggaaa
      481 cactctcctc agctggccca ggccatagtt gactgcggag ctctggatac actggtgatc
      541 tgcttggagg attttgaccc tggggtcaag gaggctgcag cctgggcact cggatacatt
      601 gcaagacaca acacagaatt atctcaagcc gtggtggatg caggagcaat tcctctttta
      661 gtcctttgta tccaggagcc agaaattgct ttgaaaagga tcgctgcctc ggccctcagt
      721 gatatctcaa agcactcccc agagttagca cagacggtgg tggacgcagg agctattgct
      781 cacttagccc agatgatcct caaccctgat gcaaaactga agcgccaggt cctctcagct
      841 ctcagtcaga ttgcaaagca ttctgtggac ttggcagaga tggttgttga agcagagatt
      901 ttccctgttg tactaacctg cctgaaggac aaagatgaat atgtgaagaa aaatgcttgc
      961 accttaatca gagagattgc aaaacacacg cctgagctct cgcagctgat tgttaatgca
     1021 ggaggcgtgg ctgccgtgat cgattgcatt gggtcctgca aagggaacat acggctgcct
     1081 ggcatcatga tgctgggtta cgtggctgct cattctgaga acctggccat ggcggtgatc
     1141 atctccaagg gtgtacccca gttgtcaatc tgcctgtcag aagaaccaga agatcatatt
     1201 aaggctgctg ctgcctgggc cttggggcag ctgggaagac acactccaga gcatgccagg
     1261 gctgtggctg tcacaaacac gctgccagtc ctgctgtctt tgtacatgtc accagagagc
     1321 tctgaggacc tacagctcaa aagtaaaaaa gccataaaga atatccttca gaaatgcacc
     1381 tacctcccag cccttgagcc gttcctgtac gacgcacctc ccaatatcct gaaacatgtt
     1441 gttgggcagt tcagtaaggt gctgccacat gacagcaaag cccggcggct tttcgtgact
     1501 agtggtggac ttaaaaaggt tcaggagata aaggcagaac caggctctct cctgcaggaa
     1561 tacatcaaca gcatcaacaa ttgttaccca gaggaaatag taaggtatta ttcacctgga
     1621 tactccgata cacttctgca gagggtggac agctaccaac cactgtgaac aaagcatatt
     1681 tttattctcc tcggttttcc aggagagtgt gaataatgat tgttaacatt atctgaaagt
     1741 acattcttta ctcagtgtaa gataccttta gataatgttt tctaaatgta cgtaacagtt
     1801 taaggttagt ggctggacta tatgaaggac aagtcatagt catttgcata cacaggaatt
     1861 tctctaaagc tagacttaaa aagacattaa aaggtttaaa acacttgcta ttgtcttgct
     1921 gaaaaaacac atgccagcag cactaaccca gccttcaatt agatgagaga cactgttcag
     1981 caacactcag gccatttcct catagtaagc acagacggtg acaacctgcc tttcaagtta
     2041 cacagaagca cctggaagaa gagctgaaaa atgaacctga cgacagggca ttgcaaccct
     2101 tgtataagag gtttggatgc taatgcagaa gctaagatag gctgttaggt ttggttttct
     2161 aagaatgtag gcagtcctat acattgtcac tggttttgca gcccttctcc ctctccctga
     2221 agggtcccaa gcattgcatt ctggaatcag tgctcccatt ttttcttgag gtttcatact
     2281 caaaggcacg gaacttttgg ccacagtgtt ctcctggctt aggctcacat taccaaatgc
     2341 ccaagtcatg ttttccgatg ttgatatcat ttgagtggaa cacaccagac tcagacttta
     2401 ttcacatttt atattagatg tagtaatggg gtcaaagaag atttcaataa agtgttaaaa
     2461 ccagcaaaaa aaaaaaaaaa aaa
//
