LOCUS       NM_001409584            2732 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus sperm associated antigen 6 (Spag6), transcript variant
            2, mRNA.
ACCESSION   NM_001409584 XM_006497502
VERSION     NM_001409584.1
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Man,Y., Li,W., Yap,Y.T., Kearney,A., Yee,S.P., Strauss,J.F. 3rd,
            Harding,P., Song,S., Zhang,L. and Zhang,Z.
  TITLE     Generation of floxed Spag6l mice and disruption of the gene by
            crossing to a Hprt-Cre line
  JOURNAL   Genesis 61 (3-4), e23512 (2023)
   PUBMED   37058328
REFERENCE   3  (bases 1 to 2732)
  AUTHORS   Yap,Y.T., Li,W., Zhou,Q., Haj-Diab,S., Chowdhury,D.D., Vaishnav,A.,
            Harding,P., Williams,D.C. Jr., Edwards,B.F., Strauss,J.F. 3rd and
            Zhang,Z.
  TITLE     The Ancient and Evolved Mouse Sperm-Associated Antigen 6 Genes Have
            Different Biologic Functions In Vivo
  JOURNAL   Cells 11 (3), 336 (2022)
   PUBMED   35159146
  REMARK    GeneRIF: The Ancient and Evolved Mouse Sperm-Associated Antigen 6
            Genes Have Different Biologic Functions In Vivo.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2732)
  AUTHORS   Li,X., Zhang,D., Xu,L., Liu,W., Zhang,N., Strauss,J.F. 3rd,
            Zhang,Z. and Wang,H.
  TITLE     Sperm-associated antigen 6 (Spag6) mutation leads to vestibular
            dysfunction in mice
  JOURNAL   J Pharmacol Sci 147 (4), 325-330 (2021)
   PUBMED   34663514
  REMARK    GeneRIF: Sperm-associated antigen 6 (Spag6) mutation leads to
            vestibular dysfunction in mice.
REFERENCE   5  (bases 1 to 2732)
  AUTHORS   Luo,J., Mu,J., Zhang,M., Zhao,B. and Liu,L.
  TITLE     SPAG6-silencing enhances decitabine-induced apoptosis and
            demethylation of PTEN in SKM-1 cells and in a xenograft mouse model
  JOURNAL   Leuk Lymphoma 62 (9), 2242-2252 (2021)
   PUBMED   33843428
  REMARK    GeneRIF: SPAG6-silencing enhances decitabine-induced apoptosis and
            demethylation of PTEN in SKM-1 cells and in a xenograft mouse
            model.
REFERENCE   6  (bases 1 to 2732)
  AUTHORS   Alciaturi,J., Anesetti,G., Irigoin,F., Skowronek,F. and Sapiro,R.
  TITLE     Distribution of sperm antigen 6 (SPAG6) and 16 (SPAG16) in mouse
            ciliated and non-ciliated tissues
  JOURNAL   J Mol Histol 50 (3), 189-202 (2019)
   PUBMED   30911868
  REMARK    GeneRIF: In this work, we performed a quantitative analysis of the
            expression of Spag6 and Spag16 genes in parallel with the
            immune-localization of the proteins in several tissues of adult
            mice.
REFERENCE   7  (bases 1 to 2732)
  AUTHORS   Hu,X., Yan,R., Cheng,X., Song,L., Zhang,W., Li,K. and Zhao,S.
  TITLE     The function of sperm-associated antigen 6 in neuronal
            proliferation and differentiation
  JOURNAL   J Mol Histol 47 (6), 531-540 (2016)
   PUBMED   27566703
  REMARK    GeneRIF: our data indicate that Spag6 plays an essential role in
            the process of neuronal proliferation and differentiation.
REFERENCE   8  (bases 1 to 2732)
  AUTHORS   Yan,R., Hu,X., Zhang,Q., Song,L., Zhang,M., Zhang,Y. and Zhao,S.
  TITLE     Spag6 Negatively Regulates Neuronal Migration During Mouse Brain
            Development
  JOURNAL   J Mol Neurosci 57 (4), 463-469 (2015)
   PUBMED   26130477
  REMARK    GeneRIF: Spag6 was continuously expressed in the developing
            cerebral cortex.  Overexpression of Spag6 caused a significant
            decrease in neurite number and length of cortical neurons.
REFERENCE   9  (bases 1 to 2732)
  AUTHORS   Li,W., Mukherjee,A., Wu,J., Zhang,L., Teves,M.E., Li,H.,
            Nambiar,S., Henderson,S.C., Horwitz,A.R., Strauss,J.F. III, Fang,X.
            and Zhang,Z.
  TITLE     Sperm Associated Antigen 6 (SPAG6) Regulates Fibroblast Cell
            Growth, Morphology, Migration and Ciliogenesis
  JOURNAL   Sci Rep 5, 16506 (2015)
   PUBMED   26585507
  REMARK    GeneRIF: Data show reduced motility of sperm associated antigen 6
            (Spag6)-deficient mouse embryonic fibroblasts (MEFs).
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 2732)
  AUTHORS   Qiu,H., Golas,A., Grzmil,P. and Wojnowski,L.
  TITLE     Lineage-specific duplications of Muroidea Faim and Spag6 genes and
            atypical accelerated evolution of the parental Spag6 gene
  JOURNAL   J Mol Evol 77 (3), 119-129 (2013)
   PUBMED   24071998
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL928680.5.
            
            On Jul 20, 2022 this sequence version replaced XM_006497502.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR3363658.3062936.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-501               AL928680.5         41119-41619
            502-542             AL928680.5         42275-42315
            543-640             AL928680.5         45621-45718
            641-1009            AL928680.5         45848-46216
            1010-1105           AL928680.5         46290-46385
            1106-1272           AL928680.5         57589-57755
            1273-1456           AL928680.5         62763-62946
            1457-1662           AL928680.5         79062-79267
            1663-1836           AL928680.5         80121-80294
            1837-1989           AL928680.5         81235-81387
            1990-2181           AL928680.5         84325-84516
            2182-2298           AL928680.5         89875-89991
            2299-2444           AL928680.5         92581-92726
            2445-2732           AL928680.5         96585-96872
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 12.9 cM"
     gene            1..2732
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /note="sperm associated antigen 6"
                     /db_xref="GeneID:381350"
                     /db_xref="MGI:MGI:3040687"
     exon            1..501
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            502..542
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            543..640
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            641..1009
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     CDS             985..2514
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /note="isoform 1 is encoded by transcript variant 2; sperm
                     associated antigen 6 like"
                     /codon_start=1
                     /product="sperm-associated antigen 6 isoform 1"
                     /protein_id="NP_001396513.1"
                     /db_xref="GeneID:381350"
                     /db_xref="MGI:MGI:3040687"
                     /translation="MSQRQVLQVFELYQNARTRFVQMVAEQATKPQNIETLQNAGIMS
                     LLRPLLLDVVPTIQQTAALALGRLANYNDDLAEAVVKGDILPQLVYSLAEQNCVYKKA
                     AAFVLRAVGKHSPQLAQATVDCGALDSLVICLEDFDPGVKEAAAWALAYIARHNAELS
                     QAVVDAGAVPLLVLCIQEPETALKRIAASALSDISKHSPELAQTVVDVGAIAHLAQMI
                     LNPDEKLKQQVLSALSHIAKHSVDLAEMVVEAEIFPVVLTCLKDKDDFVKKNACTLIR
                     EIAKHTPELSQLIVNAGGVAAVIDCIGSCKGNIRLPGIMMLGYMAAHSENLAMAVIIS
                     KGVPQLSDCLSEEPEDHIKAAAAWALGQVGRHTPEHARAVAITNTLPVLLALYMSPES
                     SEDLQVKSKKAIKNIIQKCTYLPALEPFLYDAPPNILKYVAGQFSKVLPHDSKARRLF
                     VTSGGLKKIQEIKAEPGSILQEYINNINSCYPEEIVRYYSPGYSDILLQRIDSYQPLI
                     N"
     exon            1010..1105
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            1106..1272
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1456
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            1457..1662
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1836
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1989
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            1990..2181
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            2182..2298
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2444
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     exon            2445..2732
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2591..2596
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /note="hexamer: AACAAG"
     polyA_site      2623
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /note="major polyA site"
     regulatory      2709..2714
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /note="hexamer: AATAAA"
     regulatory      2713..2718
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /note="hexamer: AAGAAA"
     polyA_site      2729
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
     polyA_site      2732
                     /gene="Spag6"
                     /gene_synonym="Spag6l"
                     /note="major polyA site"
ORIGIN      
        1 acccttctcc tcctctcccc tcctaaataa gctactgtgt ctggtccctt cctctgggac
       61 ccggtgtacg ccccgggtga actgagctgg ctgcggggga cctcgcaggg ttctcacaca
      121 gtcctggcaa agggacctgg agcacttccc aaccctgccc gccgcttggg agcgtccccg
      181 gaggagaccc gcccggcagg cccagcctag cggccgcggg gcgtttcttg gttgctccca
      241 ggctgcggcc tggcctctcg gttctgggcc gagtcggcgg aggcctcgcc cgcgtcctgg
      301 ccgcgctctt cccaggcgca ccgccggaaa ggttgggggg aggcggcgac tcctgccgag
      361 actcctagcg acttgcaaag gaggcctggc cacctaaccc aaagggaagg agaggcctct
      421 cttcggtcag ccccgcaggc tccaaggccc gagagcggcc ggaggccaga gcggggaccg
      481 gaggccgtgg aggcaggcgg gccatgctgg ctttggagca agttttaata aagaaagctc
      541 tcactctcta agtgtcctta attgttgtta atatatcgta attcagtgta tggacacgat
      601 tgctgtgact ctccaggaat tcggaaatca gaagtgccag cccctagccc gctcgcgctg
      661 cagtcgcgct gcagtcggga aaaccctgcc tcgtggatgg gtcggcatag accaagtcct
      721 cccgggagac tctggcgccc tgtgcaccgc ctacagggaa gtgacaaccg acggactgtc
      781 tcgagcaaca ctggagtccc agcttatcag gtcgtccctc caccactcca tcgaccattg
      841 tgatgtcatg cctgggactt aactgttgcg acaggacggc tgcgttccga ttccgactgg
      901 ttgcccagga gtgtgggtac acagagcagt cccgagggac gttgaggggt cgcggcgagc
      961 ggacccgaca cgcgccgcgg caccatgagc cagcggcagg tgctgcaagt gttcgagctg
     1021 taccagaacg cccggacccg gtttgtgcag atggtggcgg agcaggcgac caaaccccag
     1081 aacatcgaga cactgcagaa tgcgggtata atgtctttgc tgaggcctct tcttctggac
     1141 gtggtcccaa ccattcagca gactgctgcc ttggctctgg ggaggctggc caactacaac
     1201 gatgacttag cagaagcagt cgtgaagggt gacattcttc cacagcttgt gtattcattg
     1261 gcagagcaga attgtgtcta caagaaggca gctgcgtttg tgctgcgagc ggttggtaaa
     1321 cactctcctc agctggcgca ggccacagtt gactgtggtg ccctggactc actggtgatc
     1381 tgcttggaag attttgaccc tggagtcaag gaggctgcag cctgggcact tgcatatatt
     1441 gcaaggcata atgcagaact gtcgcaagct gtggtggatg cgggagctgt tcctctttta
     1501 gtcctctgta tccaggagcc cgagactgct ttgaaaagga tcgctgcctc ggccctcagt
     1561 gatatttcaa agcactcccc agagttagca caaacggtgg tggatgtagg agctattgct
     1621 cacttagccc agatgatcct caaccccgat gagaaactga agcaacaggt cctctcagct
     1681 ctgagtcaca tcgcgaagca ctcggtggac ctggcagaga tggttgtgga ggcagagatt
     1741 ttcccagttg ttctcacctg tctgaaggac aaagatgact tcgtgaagaa gaatgcttgt
     1801 actctgatta gagagatcgc aaaacataca ccagagctct cgcagctgat tgttaatgcc
     1861 ggaggtgtgg ctgccgtgat cgactgcatc gggtcctgca aagggaacat acggctgcct
     1921 ggcatcatga tgctgggtta catggctgct cattctgaga acctggccat ggcggtgatc
     1981 atctccaagg gtgtacccca gttgtcagac tgcctgtcag aagaaccgga agatcatatt
     2041 aaggctgcgg ctgcctgggc tctagggcag gttgggaggc acactccaga acatgctcgg
     2101 gctgtcgcca tcacaaacac gttgcccgtg ctgctggctt tgtacatgtc cccagagagc
     2161 tctgaggacc tgcaggtgaa aagtaaaaag gccataaaga atatcatcca aaaatgcacc
     2221 tacctcccgg cgcttgagcc gtttctctat gatgcgcctc ccaatatcct gaagtatgtc
     2281 gctggacagt tcagtaaggt gctgccacat gacagcaaag ctcgtcggct ttttgtaaca
     2341 agtggtggac ttaaaaagat ccaagagatc aaagcagaac ctggttccat cctccaagag
     2401 tatatcaaca acatcaacag ctgctaccca gaggaaatag taaggtacta ttctcctgga
     2461 tactcagaca tacttctgca gaggatcgac agctatcagc ctcttattaa ctgaacgaag
     2521 tatattataa tactcagatt cacaggagag taaggataac aattgttaaa gatctttcta
     2581 aaatgtctga aacaagtaca ccctagcttt cttcgatgtg aaatgtttta atgttctcta
     2641 tatttatgta atcctttaga aattattagc tgcaatgtat gaaaagacta gttataatca
     2701 tttgcatgaa taaagaaaaa cttaaaagtc aa
//
