LOCUS       NM_017407               3825 bp    mRNA    linear   ROD 28-AUG-2024
DEFINITION  Mus musculus sperm associated antigen 5 (Spag5), transcript variant
            1, mRNA.
ACCESSION   NM_017407
VERSION     NM_017407.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Guo,L., Yuan,H., Zhu,H., Zhou,J., Wan,Z. and Zhou,Y.
  TITLE     SPAG5 deficiency activates autophagy to reduce atherosclerotic
            plaque formation in ApoE-/- mice
  JOURNAL   BMC Cardiovasc Disord 24 (1), 275 (2024)
   PUBMED   38807081
  REMARK    GeneRIF: SPAG5 deficiency activates autophagy to reduce
            atherosclerotic plaque formation in ApoE[-/-] mice.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Mora-Bermudez,F., Kanis,P., Macak,D., Peters,J., Naumann,R.,
            Xing,L., Sarov,M., Winkler,S., Oegema,C.E., Haffner,C.,
            Wimberger,P., Riesenberg,S., Maricic,T., Huttner,W.B. and Paabo,S.
  TITLE     Longer metaphase and fewer chromosome segregation errors in modern
            human than Neanderthal brain development
  JOURNAL   Sci Adv 8 (30), eabn7702 (2022)
   PUBMED   35905187
REFERENCE   3  (bases 1 to 3825)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3825)
  AUTHORS   Lau,E.O., Damiani,D., Chehade,G., Ruiz-Reig,N., Saade,R.,
            Jossin,Y., Aittaleb,M., Schakman,O., Tajeddine,N., Gailly,P. and
            Tissir,F.
  TITLE     DIAPH3 deficiency links microtubules to mitotic errors, defective
            neurogenesis, and brain dysfunction
  JOURNAL   Elife 10, e61974 (2021)
   PUBMED   33899739
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3825)
  AUTHORS   Ulmke,P.A., Sakib,M.S., Ditte,P., Sokpor,G., Kerimoglu,C., Pham,L.,
            Xie,Y., Mao,X., Rosenbusch,J., Teichmann,U., Nguyen,H.P.,
            Fischer,A., Eichele,G., Staiger,J.F. and Tuoc,T.
  TITLE     Molecular Profiling Reveals Involvement of ESCO2 in Intermediate
            Progenitor Cell Maintenance in the Developing Mouse Cortex
  JOURNAL   Stem Cell Reports 16 (4), 968-984 (2021)
   PUBMED   33798452
REFERENCE   6  (bases 1 to 3825)
  AUTHORS   Xue,J., Tarnasky,H.A., Rancourt,D.E. and van Der Hoorn,F.A.
  TITLE     Targeted disruption of the testicular SPAG5/deepest protein does
            not affect spermatogenesis or fertility
  JOURNAL   Mol Cell Biol 22 (7), 1993-1997 (2002)
   PUBMED   11884588
REFERENCE   7  (bases 1 to 3825)
  AUTHORS   Mack,G.J. and Compton,D.A.
  TITLE     Analysis of mitotic microtubule-associated proteins using mass
            spectrometry identifies astrin, a spindle-associated protein
  JOURNAL   Proc Natl Acad Sci U S A 98 (25), 14434-14439 (2001)
   PUBMED   11724960
REFERENCE   8  (bases 1 to 3825)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (bases 1 to 3825)
  AUTHORS   Shao,X., Xue,J. and van der Hoorn,F.A.
  TITLE     Testicular protein Spag5 has similarity to mitotic spindle protein
            Deepest and binds outer dense fiber protein Odf1
  JOURNAL   Mol Reprod Dev 59 (4), 410-416 (2001)
   PUBMED   11468777
REFERENCE   10 (bases 1 to 3825)
  AUTHORS   Nehls,M., Luno,K., Schorpp,M., Pfeifer,D., Krause,S.,
            Matysiak-Scholze,U., Dierbach,H. and Boehm,T.
  TITLE     YAC/P1 contigs defining the location of 56 microsatellite markers
            and several genes across a 3.4-cM interval on mouse chromosome 11
  JOURNAL   Mamm Genome 6 (5), 321-331 (1995)
   PUBMED   7626882
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591070.19.
            
            On May 1, 2018 this sequence version replaced NM_017407.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052672.1, AK076619.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-120               AL591070.19        48280-48399         c
            121-246             AL591070.19        47970-48095         c
            247-1241            AL591070.19        44950-45944         c
            1242-1419           AL591070.19        44670-44847         c
            1420-1494           AL591070.19        36801-36875         c
            1495-1587           AL591070.19        36550-36642         c
            1588-1722           AL591070.19        36343-36477         c
            1723-1842           AL591070.19        36051-36170         c
            1843-1935           AL591070.19        35734-35826         c
            1936-2049           AL591070.19        35534-35647         c
            2050-2143           AL591070.19        35192-35285         c
            2144-2291           AL591070.19        34882-35029         c
            2292-2394           AL591070.19        34700-34802         c
            2395-2528           AL591070.19        34348-34481         c
            2529-2670           AL591070.19        33949-34090         c
            2671-2782           AL591070.19        30412-30523         c
            2783-2869           AL591070.19        29918-30004         c
            2870-2990           AL591070.19        29538-29658         c
            2991-3102           AL591070.19        29318-29429         c
            3103-3183           AL591070.19        28867-28947         c
            3184-3339           AL591070.19        28573-28728         c
            3340-3414           AL591070.19        28418-28492         c
            3415-3495           AL591070.19        28224-28304         c
            3496-3825           AL591070.19        27533-27862         c
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 46.74 cM"
     gene            1..3825
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /note="sperm associated antigen 5"
                     /db_xref="GeneID:54141"
                     /db_xref="MGI:MGI:1927470"
     exon            1..120
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     CDS             70..3567
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /note="astrin; microtubule-associated protein; mitotic
                     spindle-associated protein p126"
                     /codon_start=1
                     /product="sperm-associated antigen 5"
                     /protein_id="NP_059103.1"
                     /db_xref="CCDS:CCDS25098.1"
                     /db_xref="GeneID:54141"
                     /db_xref="MGI:MGI:1927470"
                     /translation="MWRVKTLNLGLSPSPQKGKPAMSTPLRELKLQPEALADSGKGPS
                     MISALTPYLCRLELKERCNNSSPVDFINTENNFLSEQFSHPSTHIEACQRESDPTPES
                     NSLFHTLEEAIETVDDFVVDPRDDSIVESMVLLPFSLGQQQDLMLQAHLDTTAERTKS
                     SLNESLGLEDLVGKEVAPCVEDSLTEIVAIRPEQPTFQDPPLGPSDTEDAPVDLVPSE
                     NVLNFSLARLSPSAVLAQDFSVDHVDPGEETVENRVLQEMETSFPTFPEEAELGDQAP
                     AANAEAVSPLYLTSSLVEMGPREAPGPTVEDASRIPGLESETWMSPLAWLEKGVNTSV
                     MLQNLRQSLSFSSVLQDAAVGNTPLATCSVGTSFTPPAPLEVGTKDSTSETERLLLGC
                     RPPDLATLSRHDLEENLLNSLVLLEVLSHQLQAWKSQLTVPHREARDSSTQTDSSPCG
                     VTKTPKHLQDSKEIRQALLQARNVMQSWGLVSGDLLSLLHLSLTHVQEGRVTVSQESQ
                     RSKTLVSSCSRVLKKLKAKLQSLKTECEEARHSKEMALKGKAAAEAVLEAFRAHASQR
                     ISQLEQGLTSMQEFRGLLQEAQTQLIGLHTEQKELAQQTVSLSSALQQDWTSVQLNYG
                     IWAALLSWSRELTKKLTAKSRQALQERDAAIEEKKQVVKEVEQVSAHLEDCKGQIEQL
                     KLENSRLTADLSAQLQILTSTESQLKEVRSQHSRCVQDLAVKDELLCQLTQSNKEQAT
                     QWQKEEMELKHIQAELLQQQAVLAKEVQDLRETVEFIDEESQVAHRELGQIESQLKVT
                     LELLRERSLQCETLRDTVDSLRAELASTEAKHEKQALEKTHQHSQELRLLAEQLQSLT
                     LFLQAKLKENKAESEIILPSTGSAPAQEHPLSNDSSISEQTPTAAVDEVPEPAPVPLL
                     GSVKSAFTRVASMASFQPTETPDLEKSLAEMSTVLQELKSLCSLLQESKEEATGVLQR
                     EICELHSRLQAQEEEHQEALKAKEADMEKLNQALCLLRKNEKELLEVIQKQNEKILGQ
                     IDKSGQLINLREEVTQLTQSLRRAETETKVLQEALEGQLDPSCQLMATNWIQEKVFLS
                     QEVSKLRVMFLEMKTEKEQLMDKYLSHRHILEENLRRSDTELKKLDDTIQHVYETLLS
                     IPETMKSCKELQGLLEFLS"
     exon            121..246
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            247..1241
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            1242..1419
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            1420..1494
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1587
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1722
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            1723..1842
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            1843..1935
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..2049
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            2050..2143
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            2144..2291
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            2292..2394
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            2395..2528
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            2529..2670
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            2671..2782
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            2783..2869
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            2870..2990
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            2991..3102
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            3103..3183
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3339
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            3340..3414
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            3415..3495
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     exon            3496..3825
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3803..3808
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /note="hexamer: AATAAA"
     polyA_site      3822
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /note="major polyA site"
ORIGIN      
        1 gggttcaaat agaggcggcg ggtgaaagtg gccatttcga cttggtctgg ttaagtgatc
       61 gagttgaaaa tgtggagggt gaaaacactg aatctcggtt tgtcgccttc gccccaaaag
      121 gggaagccag ctatgagtac tcctctccga gagcttaaac tacagcccga ggccctcgcc
      181 gattcaggga aaggtccctc tatgatctcc gcgctgaccc catacctgtg caggctggag
      241 ctgaaggaaa gatgcaacaa ctcatctcca gtggatttta tcaatactga gaacaacttc
      301 ctttcagaac agttcagcca tccttcaacg cacatagaag cttgccagcg tgaatccgat
      361 ccaactcctg aaagcaactc tcttttccat actttggagg aagcaataga aacagtggat
      421 gactttgtcg tggacccaag agatgatagt atagtggagt ccatggttct tctgcccttt
      481 tcattagggc agcagcaaga cctgatgctc caggctcact tagataccac agcagagaga
      541 actaaaagct ctctaaatga atctttgggg ctagaagatc tggtggggaa ggaggtggca
      601 ccttgtgtgg aagacagcct aacagaaatt gttgctatta ggcccgagca acctacattt
      661 caggaccctc cgttaggacc cagtgacact gaagatgcac ctgtggactt agttccttct
      721 gaaaatgtcc tgaatttctc tctggctcgt ctttctcctt cagctgtcct ggcccaggat
      781 ttctctgtcg atcatgtcga tccaggggag gaaaccgtag agaacagagt cctacaggaa
      841 atggaaacga gcttccccac attccctgag gaggccgaat tgggagacca agcacctgct
      901 gcaaatgcag aagccgtctc acccttgtac ctgacatcaa gtctagtgga gatggggccc
      961 cgggaagccc caggcccaac agtagaagac gccagtagga ttcctggcct tgagtcagag
     1021 acttggatgt ccccattggc ctggctagaa aagggtgtga atacatcggt catgctacaa
     1081 aacctccgcc agagcttatc cttttcttct gtgcttcagg atgctgccgt tggcaacaca
     1141 cccctcgcca cgtgttctgt gggcacttcc tttactcctc cagcaccact ggaggtaggc
     1201 accaaggaca gtacttcaga gacagagcgc ctcctcttgg gctgccgtcc tccagatctg
     1261 gctaccttgt cccgacacga cttggaagag aacctgttga actctcttgt cttgctggaa
     1321 gttctttccc accagctaca agcctggaag agccagttga ctgtccctca ccgggaagct
     1381 cgagacagta gtacacagac tgacagctct ccttgtgggg tcactaagac acctaaacat
     1441 cttcaggaca gcaaggagat tagacaggct ctactgcaag ccaggaatgt catgcaatca
     1501 tggggtctag tctctggaga cctgttgtcc ttgcttcacc tgtccctaac acacgtgcaa
     1561 gaaggcagag tgacagtgag tcaggagtct cagcggtcaa aaaccctggt ttcctcctgt
     1621 tctcgtgtgc tgaagaaatt gaaggcaaag ctgcagagcc tcaaaacaga atgtgaggag
     1681 gcaaggcaca gcaaggaaat ggcccttaaa ggcaaggctg cggcagaggc agtactggag
     1741 gctttccgtg cacatgccag ccagcgcatc agccaactgg aacagggtct cacatctatg
     1801 caggaattca gaggccttct acaggaggca cagacccaac tgatagggct tcacacagaa
     1861 caaaaggagc tggctcagca gacagtgagt ctgagttctg ccttgcagca ggactggaca
     1921 tcagtccaat tgaattatgg aatatgggca gctttgctga gttggtctcg agaactcacc
     1981 aagaaactca cagccaagag ccggcaggcc cttcaggaac gtgatgctgc aattgaagaa
     2041 aagaagcagg ttgtgaagga agtggaacaa gtctctgccc atttagagga ctgtaaaggt
     2101 caaatcgaac aactgaagtt ggaaaatagt cgccttactg cagatctctc ggctcagctg
     2161 cagattctga ctagcacaga gagtcagcta aaggaagtac ggagtcagca ttcccgctgt
     2221 gtccaggacc tggccgtgaa ggatgaattg ctgtgtcaac ttacccagag caacaaggag
     2281 caggctactc aatggcaaaa ggaagaaatg gaactgaaac acatacaagc agaactgcta
     2341 cagcagcagg ctgtgttggc taaggaggtc caggacctga gggagactgt ggagtttata
     2401 gatgaagaaa gtcaagttgc tcaccgggag ctgggccaga ttgagagtca gttgaaagtc
     2461 acgctagagt tactgcggga gcgcagcctg cagtgtgaga ccctcaggga cactgtggac
     2521 agcctgaggg ctgagctggc cagcactgaa gcaaagcatg agaaacaggc cctagagaag
     2581 acacaccagc attctcaaga gctgcggcta ctggctgagc agctgcagag tctcaccctc
     2641 ttcttacagg caaaactcaa ggagaacaag gctgaatcag agatcattct gcccagcaca
     2701 ggctctgctc cagcccagga acaccctctg tccaatgaca gcagcatctc agaacagacc
     2761 ccgacagcag cagtagatga agtgccagaa ccagctcctg tgccattgct tggaagtgtt
     2821 aagagtgctt tcacccgagt agcctcaatg gcttcctttc agcctacaga gaccccagac
     2881 ttggagaaga gcctggcaga aatgagtact gtgttacaag agcttaagag cctgtgttcc
     2941 ctgctgcaag agtctaaaga ggaggccact ggggtcctgc agagggaaat ctgtgaacta
     3001 cactcgagac tacaggccca agaagaagag catcaggaag ccctgaaggc aaaggaagca
     3061 gacatggaga agctgaacca ggccttgtgc ttgctccgca agaatgagaa ggagctcctg
     3121 gaagtgatac agaagcagaa cgagaagatc ctggggcaaa tagacaagag cggccagctc
     3181 ataaacctca gagaggaggt gacccagctc acacagtcac ttcggcgtgc agagacagag
     3241 actaaagtgc tccaggaagc cctggaaggc cagctagatc ccagctgcca gctgatggct
     3301 actaactgga tccaggaaaa agtgtttctc tcacaggagg tgagcaagct gagggttatg
     3361 ttcctggaga tgaaaactga gaaggaacag ctgatggaca agtatctgag ccataggcac
     3421 atcctggagg agaatcttcg gcgctctgac acagagttaa agaaactcga tgacacaatt
     3481 cagcatgtct atgagactct gttgtctatc ccagagacta tgaagagttg caaggagtta
     3541 caaggattgc tagaatttct gagctaagaa actgaaaata ttggaatgca gtggtgtgca
     3601 cctgtagttc cagctgctcc agacgccaaa gttggagtgc tgccaaatcc caggaatttg
     3661 ggaacctaac acagacaaca cagcaaaaac cctgaaaaat gaattccact ccactccagt
     3721 ttccttcagg gctctttatc ccaaccctta tcccaataat aagaccaatt ggcatagagc
     3781 cgaccatgtt ttatgttatt taaataaagt gtatttaatg tagtc
//
