LOCUS       NP_059103               1165 aa            linear   ROD 27-APR-2025
DEFINITION  sperm-associated antigen 5 [Mus musculus].
ACCESSION   NP_059103
VERSION     NP_059103.1
DBSOURCE    REFSEQ: accession NM_017407.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 1165)
  AUTHORS   Guo,L., Yuan,H., Zhu,H., Zhou,J., Wan,Z. and Zhou,Y.
  TITLE     SPAG5 deficiency activates autophagy to reduce atherosclerotic
            plaque formation in ApoE-/- mice
  JOURNAL   BMC Cardiovasc Disord 24 (1), 275 (2024)
   PUBMED   38807081
  REMARK    GeneRIF: SPAG5 deficiency activates autophagy to reduce
            atherosclerotic plaque formation in ApoE[-/-] mice.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1165)
  AUTHORS   Mora-Bermudez,F., Kanis,P., Macak,D., Peters,J., Naumann,R.,
            Xing,L., Sarov,M., Winkler,S., Oegema,C.E., Haffner,C.,
            Wimberger,P., Riesenberg,S., Maricic,T., Huttner,W.B. and Paabo,S.
  TITLE     Longer metaphase and fewer chromosome segregation errors in modern
            human than Neanderthal brain development
  JOURNAL   Sci Adv 8 (30), eabn7702 (2022)
   PUBMED   35905187
REFERENCE   3  (residues 1 to 1165)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (residues 1 to 1165)
  AUTHORS   Lau,E.O., Damiani,D., Chehade,G., Ruiz-Reig,N., Saade,R.,
            Jossin,Y., Aittaleb,M., Schakman,O., Tajeddine,N., Gailly,P. and
            Tissir,F.
  TITLE     DIAPH3 deficiency links microtubules to mitotic errors, defective
            neurogenesis, and brain dysfunction
  JOURNAL   Elife 10, e61974 (2021)
   PUBMED   33899739
  REMARK    Publication Status: Online-Only
REFERENCE   5  (residues 1 to 1165)
  AUTHORS   Ulmke,P.A., Sakib,M.S., Ditte,P., Sokpor,G., Kerimoglu,C., Pham,L.,
            Xie,Y., Mao,X., Rosenbusch,J., Teichmann,U., Nguyen,H.P.,
            Fischer,A., Eichele,G., Staiger,J.F. and Tuoc,T.
  TITLE     Molecular Profiling Reveals Involvement of ESCO2 in Intermediate
            Progenitor Cell Maintenance in the Developing Mouse Cortex
  JOURNAL   Stem Cell Reports 16 (4), 968-984 (2021)
   PUBMED   33798452
REFERENCE   6  (residues 1 to 1165)
  AUTHORS   Xue,J., Tarnasky,H.A., Rancourt,D.E. and van Der Hoorn,F.A.
  TITLE     Targeted disruption of the testicular SPAG5/deepest protein does
            not affect spermatogenesis or fertility
  JOURNAL   Mol Cell Biol 22 (7), 1993-1997 (2002)
   PUBMED   11884588
REFERENCE   7  (residues 1 to 1165)
  AUTHORS   Mack,G.J. and Compton,D.A.
  TITLE     Analysis of mitotic microtubule-associated proteins using mass
            spectrometry identifies astrin, a spindle-associated protein
  JOURNAL   Proc Natl Acad Sci U S A 98 (25), 14434-14439 (2001)
   PUBMED   11724960
REFERENCE   8  (residues 1 to 1165)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   9  (residues 1 to 1165)
  AUTHORS   Shao,X., Xue,J. and van der Hoorn,F.A.
  TITLE     Testicular protein Spag5 has similarity to mitotic spindle protein
            Deepest and binds outer dense fiber protein Odf1
  JOURNAL   Mol Reprod Dev 59 (4), 410-416 (2001)
   PUBMED   11468777
REFERENCE   10 (residues 1 to 1165)
  AUTHORS   Nehls,M., Luno,K., Schorpp,M., Pfeifer,D., Krause,S.,
            Matysiak-Scholze,U., Dierbach,H. and Boehm,T.
  TITLE     YAC/P1 contigs defining the location of 56 microsatellite markers
            and several genes across a 3.4-cM interval on mouse chromosome 11
  JOURNAL   Mamm Genome 6 (5), 321-331 (1995)
   PUBMED   7626882
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591070.19.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052672.1, AK076619.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1165
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 46.74 cM"
     Protein         1..1165
                     /product="sperm-associated antigen 5"
                     /note="astrin; microtubule-associated protein; mitotic
                     spindle-associated protein p126"
                     /calculated_mol_wt=129862
     Region          1..23
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q7TME2.1)"
     Site            12
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q7TME2.1)"
     Site            14
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q7TME2.1)"
     Site            66
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96R06; propagated from
                     UniProtKB/Swiss-Prot (Q7TME2.1)"
     Site            161
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96R06; propagated from
                     UniProtKB/Swiss-Prot (Q7TME2.1)"
     Site            321
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96R06; propagated from
                     UniProtKB/Swiss-Prot (Q7TME2.1)"
     Site            333
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96R06; propagated from
                     UniProtKB/Swiss-Prot (Q7TME2.1)"
     Site            342
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:Q96R06; propagated from
                     UniProtKB/Swiss-Prot (Q7TME2.1)"
     Region          431..457
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q7TME2.1)"
     Region          453..821
                     /region_name="Interaction with KNSTRN.
                     /evidence=ECO:0000250"
                     /note="propagated from UniProtKB/Swiss-Prot (Q7TME2.1)"
     Region          <521..>1070
                     /region_name="Smc"
                     /note="Chromosome segregation ATPase Smc [Cell cycle
                     control, cell division, chromosome partitioning]; COG1196"
                     /db_xref="CDD:440809"
     Region          875..907
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q7TME2.1)"
     Site            946
                     /site_type="phosphorylation"
                     /note="Phosphoserine, by GSK3-beta.
                     /evidence=ECO:0000250|UniProtKB:Q96R06; propagated from
                     UniProtKB/Swiss-Prot (Q7TME2.1)"
     CDS             1..1165
                     /gene="Spag5"
                     /gene_synonym="D11Bhm180e; Deepest; MAP126; Mastrin; S17"
                     /coded_by="NM_017407.3:70..3567"
                     /db_xref="CCDS:CCDS25098.1"
                     /db_xref="GeneID:54141"
                     /db_xref="MGI:MGI:1927470"
ORIGIN      
        1 mwrvktlnlg lspspqkgkp amstplrelk lqpealadsg kgpsmisalt pylcrlelke
       61 rcnnsspvdf intennflse qfshpsthie acqresdptp esnslfhtle eaietvddfv
      121 vdprddsive smvllpfslg qqqdlmlqah ldttaertks slneslgled lvgkevapcv
      181 edslteivai rpeqptfqdp plgpsdteda pvdlvpsenv lnfslarlsp savlaqdfsv
      241 dhvdpgeetv enrvlqemet sfptfpeeae lgdqapaana eavsplylts slvemgprea
      301 pgptvedasr ipglesetwm splawlekgv ntsvmlqnlr qslsfssvlq daavgntpla
      361 tcsvgtsftp paplevgtkd stseterlll gcrppdlatl srhdleenll nslvllevls
      421 hqlqawksql tvphreards stqtdsspcg vtktpkhlqd skeirqallq arnvmqswgl
      481 vsgdllsllh lslthvqegr vtvsqesqrs ktlvsscsrv lkklkaklqs lkteceearh
      541 skemalkgka aaeavleafr ahasqrisql eqgltsmqef rgllqeaqtq liglhteqke
      601 laqqtvslss alqqdwtsvq lnygiwaall swsreltkkl taksrqalqe rdaaieekkq
      661 vvkeveqvsa hledckgqie qlklensrlt adlsaqlqil tstesqlkev rsqhsrcvqd
      721 lavkdellcq ltqsnkeqat qwqkeemelk hiqaellqqq avlakevqdl retvefidee
      781 sqvahrelgq iesqlkvtle llrerslqce tlrdtvdslr aelasteakh ekqalekthq
      841 hsqelrllae qlqsltlflq aklkenkaes eiilpstgsa paqehplsnd ssiseqtpta
      901 avdevpepap vpllgsvksa ftrvasmasf qptetpdlek slaemstvlq elkslcsllq
      961 eskeeatgvl qreicelhsr lqaqeeehqe alkakeadme klnqalcllr knekellevi
     1021 qkqnekilgq idksgqlinl reevtqltqs lrraetetkv lqealegqld pscqlmatnw
     1081 iqekvflsqe vsklrvmfle mktekeqlmd kylshrhile enlrrsdtel kklddtiqhv
     1141 yetllsipet mksckelqgl lefls
//
