LOCUS       NM_139151               1502 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus sperm associated antigen 4 (Spag4), mRNA.
ACCESSION   NM_139151
VERSION     NM_139151.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1502)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1502)
  AUTHORS   Thoma,H., Grunewald,L., Braune,S., Pasch,E. and Alsheimer,M.
  TITLE     SUN4 is a spermatid type II inner nuclear membrane protein that
            forms heteromeric assemblies with SUN3 and interacts with lamin B3
  JOURNAL   J Cell Sci 136 (7) (2023)
   PUBMED   36825599
REFERENCE   3  (bases 1 to 1502)
  AUTHORS   Yang,K., Adham,I.M., Meinhardt,A. and Hoyer-Fender,S.
  TITLE     Ultra-structure of the sperm head-to-tail linkage complex in the
            absence of the spermatid-specific LINC component SPAG4
  JOURNAL   Histochem Cell Biol 150 (1), 49-59 (2018)
   PUBMED   29663073
  REMARK    GeneRIF: The results thus indicate that SPAG4, albeit not essential
            for the formation of the HTCA per se, is, nevertheless, required
            for tightening the sperm head-to-tail anchorage by provoking the
            correct attachment of the lateral parts of the basal plate to the
            implantation fossa.
REFERENCE   4  (bases 1 to 1502)
  AUTHORS   Sundberg,J.P., Dadras,S.S., Silva,K.A., Kennedy,V.E., Garland,G.,
            Murray,S.A., Sundberg,B.A., Schofield,P.N. and Pratt,C.H.
  TITLE     Systematic screening for skin, hair, and nail abnormalities in a
            large-scale knockout mouse program
  JOURNAL   PLoS One 12 (7), e0180682 (2017)
   PUBMED   28700664
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1502)
  AUTHORS   Pasch,E., Link,J., Beck,C., Scheuerle,S. and Alsheimer,M.
  TITLE     The LINC complex component Sun4 plays a crucial role in sperm head
            formation and fertility
  JOURNAL   Biol Open 4 (12), 1792-1802 (2015)
   PUBMED   26621829
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1502)
  AUTHORS   Calvi,A., Wong,A.S., Wright,G., Wong,E.S., Loo,T.H., Stewart,C.L.
            and Burke,B.
  TITLE     SUN4 is essential for nuclear remodeling during mammalian
            spermiogenesis
  JOURNAL   Dev Biol 407 (2), 321-330 (2015)
   PUBMED   26417726
  REMARK    GeneRIF: SUN4, a spermatid nuclear membrane protein has an
            essential function in coupling the manchette to the nuclear
            periphery.
REFERENCE   7  (bases 1 to 1502)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
REFERENCE   8  (bases 1 to 1502)
  AUTHORS   Shao,X., Tarnasky,H.A., Lee,J.P., Oko,R. and van der Hoorn,F.A.
  TITLE     Spag4, a novel sperm protein, binds outer dense-fiber protein Odf1
            and localizes to microtubules of manchette and axoneme
  JOURNAL   Dev Biol 211 (1), 109-123 (1999)
   PUBMED   10373309
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BY231113.1, AK028080.1, AK043812.1 and BY591699.1.
            
            On Jul 6, 2007 this sequence version replaced NM_139151.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK028080.1, SRR7974084.194.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                BY231113.1         3-33
            32-164              AK028080.1         2-134
            165-260             AK043812.1         152-247
            261-1216            AK028080.1         230-1185
            1217-1232           AK043812.1         1503-1518
            1233-1255           AK028080.1         1202-1224
            1256-1320           AK043812.1         2140-2204
            1321-1500           AK028080.1         1290-1469
            1501-1502           BY591699.1         489-490
FEATURES             Location/Qualifiers
     source          1..1502
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 77.34 cM"
     gene            1..1502
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /note="sperm associated antigen 4"
                     /db_xref="GeneID:245865"
                     /db_xref="MGI:MGI:2444120"
     exon            1..459
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..1481
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /note="sperm antigen 4; outer dense fiber-associated
                     protein SPAG4; SUN domain-containing protein 4"
                     /codon_start=1
                     /product="sperm-associated antigen 4 protein"
                     /protein_id="NP_631890.3"
                     /db_xref="CCDS:CCDS50777.1"
                     /db_xref="GeneID:245865"
                     /db_xref="MGI:MGI:2444120"
                     /translation="MRRSPRSGSAASSHNHTPNFYSENSNSSHSATSGDSNGRRSAGP
                     ELGEPEGRRARGSSCGEPALSPGMPGGDTWAGSSRPKLAPRSHNGQTACGAATVRGGA
                     SEPSGSSVVLEEQLNLLPILDLRQEMPTPRVSKSFLSLLFQVLSMVLSLAVDGLVCVC
                     REICSIRFLFTAVSLLSIFLAALWWGLLYLIPPLENEPTEMLTLSQYHHRVHSQGQQL
                     QQLQAELNKLHKEVSSVRAAHSERVAKLVFQRLNEDFVRKPDYALSSVGASIDLEKTS
                     SDYEDQNTAYFWNRLSFWNYARPPSVILEPDVFPGNCWAFEGDKGQVVIRLPGHVQLS
                     DITLQHPPPTVAHTGGASSAPRDFAVYGLQADDETEVFLGKFIFDVQKSEIQTFHLQN
                     DPPSAFPKVKIQILSNWGHPRFTCLYRVRAHGVRTSEWADDNATGVTGGPH"
     exon            460..564
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            565..631
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            632..693
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            694..737
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            738..763
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            764..872
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            873..948
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            949..1064
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1232
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            1233..1322
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
     exon            1323..1502
                     /gene="Spag4"
                     /gene_synonym="1700041K21Rik; mKIAA4118; MNCb-0953; Sun4"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 acggtgacaa cagcacagca gcgggagggt cggccgagcc gccgcggggc tcacgcaggg
       61 tgctaccctg cggcgcgagg cagctgagga agggccgagg gctttggctg cggcgacttc
      121 ggcttcccag aggggtagcc caggtcagga tgcggcggag cccccgctca ggctcggccg
      181 catcctctca taatcacaca cccaacttct acagcgagaa cagcaatagt tcccacagcg
      241 cgacttcggg ggacagcaat gggcgccggt ccgctgggcc ggagctcggg gagcccgagg
      301 gcagaagggc ccggggctcg agctgtggtg agcccgcctt aagcccagga atgcccggag
      361 gagacacatg ggcaggaagc tctcggccga agctggcgcc tcggagccac aatgggcaga
      421 ccgcctgtgg cgcggcaacc gtgaggggcg gggcctcgga accgtctgga tcttctgtag
      481 ttttggagga acaactcaac cttctcccga tcctggatct gaggcaggag atgcctaccc
      541 cgagggtgtc caagagcttc ctgagcctcc tctttcaggt gctgagcatg gtgttatccc
      601 tggcagtcga tgggctggtc tgtgtgtgca gggaaatctg ctccatccgc ttcctgttca
      661 ctgctgtgtc gctcctgagc atctttctgg cagcactctg gtgggggctc ctgtacctga
      721 tccctccttt ggagaacgaa cctacggaga tgctgactct aagccaatac caccaccgcg
      781 tgcattccca gggccagcaa ctgcagcagc tccaggcaga actgaataaa ctccacaagg
      841 aggtgtccag cgttcgtgca gcccacagtg agagagtggc caagctcgtg ttccagaggc
      901 tgaatgaaga cttcgtgcgc aaacctgact atgcactgag ctctgtggga gcctccatcg
      961 acctggagaa gacgtccagt gactacgagg accagaacac tgcctacttc tggaaccgct
     1021 tgagcttctg gaactatgcc cgcccgcctt ctgtcatatt ggagccagat gtgttccctg
     1081 gaaattgctg ggcttttgaa ggtgataaag gccaggtggt gatccgactg ccaggtcatg
     1141 tgcagttaag cgacatcacc ctgcagcatc ctccacccac tgtggcacac accggagggg
     1201 ccagcagcgc gcctcgggac tttgcagtct atgggctcca agctgatgac gagactgaag
     1261 tgttcttggg gaaattcatc tttgatgtgc agaaatctga aattcagact ttccacctcc
     1321 agaatgatcc tccttctgcc ttccccaagg tgaagattca gattctaagc aactggggcc
     1381 atccccgctt cacgtgctta tatcgcgtcc gtgctcatgg tgtgcggacc tcagagtggg
     1441 cagacgataa tgccacaggg gtcacagggg gaccccatta aaaatgccga tggttggagt
     1501 ag
//
