LOCUS       NM_029160               2144 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus sperm associated antigen 16 (Spag16), transcript
            variant 1, mRNA.
ACCESSION   NM_029160
VERSION     NM_029160.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2144)
  AUTHORS   Miyata,H., Morohoshi,A. and Ikawa,M.
  TITLE     Analysis of the sperm flagellar axoneme using gene-modified mice
  JOURNAL   Exp Anim 69 (4), 374-381 (2020)
   PUBMED   32554934
  REMARK    Review article
REFERENCE   2  (bases 1 to 2144)
  AUTHORS   Liu,Y., Zhang,L., Li,W., Huang,Q., Yuan,S., Li,Y., Liu,J.,
            Zhang,S., Pin,G., Song,S., Ray,P.F., Arnoult,C., Cho,C.,
            Garcia-Reyes,B., Knippschild,U., Strauss,J.F. and Zhang,Z.
  TITLE     The sperm-associated antigen 6 interactome and its role in
            spermatogenesis
  JOURNAL   Reproduction 158 (2), 181-197 (2019)
   PUBMED   31146259
REFERENCE   3  (bases 1 to 2144)
  AUTHORS   Alciaturi,J., Anesetti,G., Irigoin,F., Skowronek,F. and Sapiro,R.
  TITLE     Distribution of sperm antigen 6 (SPAG6) and 16 (SPAG16) in mouse
            ciliated and non-ciliated tissues
  JOURNAL   J Mol Histol 50 (3), 189-202 (2019)
   PUBMED   30911868
  REMARK    GeneRIF: In this work, we performed a quantitative analysis of the
            expression of Spag6 and Spag16 genes in parallel with the
            immune-localization of the proteins in several tissues of adult
            mice.
REFERENCE   4  (bases 1 to 2144)
  AUTHORS   Zhang,Z., Huang,Q., Wang,Z., Zou,J., Yu,Z., Strauss Iii,J.F. and
            Zhang,Z.
  TITLE     Elongin B is a binding partner of the male germ cell nuclear
            speckle protein sperm-associated antigen 16S (SPAG16S) and is
            regulated post-transcriptionally in the testis
  JOURNAL   Reprod Fertil Dev 31 (5), 962-971 (2019)
   PUBMED   30811962
REFERENCE   5  (bases 1 to 2144)
  AUTHORS   Nagarkatti-Gude,D.R., Jaimez,R., Henderson,S.C., Teves,M.E.,
            Zhang,Z. and Strauss,J.F. 3rd.
  TITLE     Spag16, an axonemal central apparatus gene, encodes a male germ
            cell nuclear speckle protein that regulates SPAG16 mRNA expression
  JOURNAL   PLoS One 6 (5), e20625 (2011)
   PUBMED   21655194
  REMARK    GeneRIF: The distinct nuclear localization of SPAG16S and its
            ability to modulate Spag16L mRNA expression suggest that SPAG16S
            plays an important role in the gene expression machinery of male
            germ cells.
REFERENCE   6  (bases 1 to 2144)
  AUTHORS   Zhang,Z., Zariwala,M.A., Mahadevan,M.M., Caballero-Campo,P.,
            Shen,X., Escudier,E., Duriez,B., Bridoux,A.M., Leigh,M.,
            Gerton,G.L., Kennedy,M., Amselem,S., Knowles,M.R. and Strauss,J.F.
            3rd.
  TITLE     A heterozygous mutation disrupting the SPAG16 gene results in
            biochemical instability of central apparatus components of the
            human sperm axoneme
  JOURNAL   Biol Reprod 77 (5), 864-871 (2007)
   PUBMED   17699735
REFERENCE   7  (bases 1 to 2144)
  AUTHORS   Zhang,Z., Tang,W., Zhou,R., Shen,X., Wei,Z., Patel,A.M.,
            Povlishock,J.T., Bennett,J. and Strauss,J.F. 3rd.
  TITLE     Accelerated mortality from hydrocephalus and pneumonia in mice with
            a combined deficiency of SPAG6 and SPAG16L reveals a functional
            interrelationship between the two central apparatus proteins
  JOURNAL   Cell Motil Cytoskeleton 64 (5), 360-376 (2007)
   PUBMED   17323374
REFERENCE   8  (bases 1 to 2144)
  AUTHORS   Zhang,Z., Kostetskii,I., Tang,W., Haig-Ladewig,L., Sapiro,R.,
            Wei,Z., Patel,A.M., Bennett,J., Gerton,G.L., Moss,S.B., Radice,G.L.
            and Strauss,J.F. 3rd.
  TITLE     Deficiency of SPAG16L causes male infertility associated with
            impaired sperm motility
  JOURNAL   Biol Reprod 74 (4), 751-759 (2006)
   PUBMED   16382026
  REMARK    GeneRIF: In sperm, SPAG16L is found in the central apparatus of the
            axoneme.  We conclude that SPAG16L is essential for sperm flagellar
            function.
REFERENCE   9  (bases 1 to 2144)
  AUTHORS   Zhang,Z., Kostetskii,I., Moss,S.B., Jones,B.H., Ho,C., Wang,H.,
            Kishida,T., Gerton,G.L., Radice,G.L. and Strauss,J.F. 3rd.
  TITLE     Haploinsufficiency for the murine orthologue of Chlamydomonas PF20
            disrupts spermatogenesis
  JOURNAL   Proc Natl Acad Sci U S A 101 (35), 12946-12951 (2004)
   PUBMED   15328412
REFERENCE   10 (bases 1 to 2144)
  AUTHORS   Zhang,Z., Sapiro,R., Kapfhamer,D., Bucan,M., Bray,J.,
            Chennathukuzhi,V., McNamara,P., Curtis,A., Zhang,M.,
            Blanchette-Mackie,E.J. and Strauss,J.F. 3rd.
  TITLE     A sperm-associated WD repeat protein orthologous to Chlamydomonas
            PF20 associates with Spag6, the mammalian orthologue of
            Chlamydomonas PF16
  JOURNAL   Mol Cell Biol 22 (22), 7993-8004 (2002)
   PUBMED   12391165
  REMARK    GeneRIF: Data describe the cloning of human and mouse Pf20 proteins
            that interact with Spag6 protein.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167331.3, AC101802.9, AC102666.13, AC164411.4, AC102458.8 and
            AC129933.13.
            
            On Oct 11, 2012 this sequence version replaced NM_029160.2.
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1; also known as Spag16L).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF490391.1, SRR7345562.664116.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN01164139
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-152               AC167331.3         58718-58869         c
            153-199             AC167331.3         42824-42870         c
            200-295             AC167331.3         41465-41560         c
            296-414             AC167331.3         32398-32516         c
            415-552             AC167331.3         27229-27366         c
            553-660             AC167331.3         15468-15575         c
            661-778             AC167331.3         12588-12705         c
            779-845             AC101802.9         155356-155422       c
            846-890             AC101802.9         147643-147687       c
            891-1000            AC101802.9         146333-146442       c
            1001-1128           AC101802.9         46006-46133         c
            1129-1272           AC102666.13        108709-108852
            1273-1458           AC102666.13        143386-143571
            1459-1585           AC164411.4         68447-68573         c
            1586-1651           AC102458.8         40400-40465         c
            1652-1778           AC102458.8         7624-7750           c
            1779-2144           AC129933.13        122778-123143       c
FEATURES             Location/Qualifiers
     source          1..2144
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 35.04 cM"
     gene            1..2144
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /note="sperm associated antigen 16"
                     /db_xref="GeneID:66722"
                     /db_xref="MGI:MGI:1913972"
     exon            1..152
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     CDS             35..1954
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sperm-associated antigen 16 protein; sperm-associated WD
                     repeat protein; pf20 protein homolog"
                     /codon_start=1
                     /product="sperm-associated antigen 16 protein isoform 1"
                     /protein_id="NP_083436.2"
                     /db_xref="CCDS:CCDS15027.1"
                     /db_xref="GeneID:66722"
                     /db_xref="MGI:MGI:1913972"
                     /translation="MAAPSGVPPLRVLEELGIGLSPTGEVTEAVTSEGAYYLEQVTIT
                     ETSEDECEYEEIPDDNFSIPEGEEDLEKAIHIIGEQARDIHILEQQTILPARNVMQEA
                     IEDFLCNFLIKMGMTRTLDCFQAEWYELIQKKGSDFKGLGNVPDVYSQVMLLETENKN
                     LKKELKHFKQAAEKAKEDLLRTQKERDFHRMHHKRIVQEKNKLIADLKGLKLHYASYE
                     PTIRVLHEKHHALLKEKMLTSLERDRAVGKISGLQATLKNIDMGHIQVPVIKGSYESA
                     SITRESGDRAGHSCEKENSSEGPTQKSLREAREEVGYKSKLKNEKKDSEFPVDMQPDP
                     NVTSCTENVSAAKFDYKLNNIFRLHELPVSCIVMHPCRDYLISCSEDRLWKMVGLPQG
                     NVLLTGSGHTDWLSGCCFHPSGSKLATSSGDSTIKLWDLNKGECTLTLEGHNHAVWSC
                     TWHSCGDFVASASLDMTSKIWDVNSERCRYTLYGHTDSVNSIEFFPFSNILLTASADK
                     TLSVWDARTGKCEQSLYGHMHSVNDATFTPRGHIIASCDARGVTKLWDFRKLIPIVSI
                     DVGPSSGNEVNFDQSGRVLAQASANGIIHLLDLKSGQIHKLVGHESEVHSVVFSHLGE
                     NLYSGGSDGTIRLWI"
     exon            153..199
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            200..295
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            296..414
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            415..552
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            553..660
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            661..778
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            779..845
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            846..890
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            891..1000
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1128
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1272
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            1273..1458
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            1459..1585
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            1586..1651
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1778
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..2144
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2120..2125
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /note="hexamer: AATAAA"
     polyA_site      2144
                     /gene="Spag16"
                     /gene_synonym="4921511D23Rik; 4930524F24Rik;
                     4930585K05Rik; Pf20; Wdr29"
                     /note="major polyA site"
ORIGIN      
        1 agtcggaggg gcaccgaggg gcgcagttgc aagcatggct gctccgtctg gggtcccgcc
       61 gctgcgggtg ctggaggagc tgggcattgg cttgtcaccc accggcgagg tgacagaggc
      121 ggtgacctcc gagggcgcct actacctgga gcaggtcacc ataacggaaa catcggaaga
      181 tgaatgcgaa tatgaagaga taccagatga caatttcagc attcctgaag gagaagaaga
      241 tctggaaaaa gcaattcaca tcattggaga gcaagccaga gacatccata ttttggaaca
      301 acaaaccatt cttcctgcaa ggaatgtaat gcaggaagcc atagaagatt ttctctgcaa
      361 tttcctgatc aaaatgggga tgaccagaac tctggactgc ttccaggccg agtggtatga
      421 gttaatacag aagaaaggaa gtgactttaa agggctcggt aatgttcccg atgtctacag
      481 ccaggtgatg cttttagaga ctgagaacaa aaacttgaag aaggaattga agcacttcaa
      541 acaggcagct gaaaaggcca aagaagatct actgagaact cagaaggaac gggacttcca
      601 tcgcatgcat cataagcgca tagtgcaaga aaagaacaag ctcattgctg acctcaaagg
      661 gctgaaattg cattatgcat catacgaacc aactataagg gtgttacatg agaagcatca
      721 tgctttattg aaggagaaaa tgctgacctc tttggaaaga gatcgagctg ttgggaagat
      781 ttctgggctt caagcaacac tgaagaacat agacatggga catattcagg ttcctgtaat
      841 taaaggatct tatgagagtg cttctatcac aagagagtca ggagacagag ctggtcacag
      901 ttgtgagaaa gaaaacagtt cagagggccc aactcagaaa agtcttcgtg aagccaggga
      961 agaagttgga tataaatcaa agttgaagaa tgagaaaaag gattcagaat ttcctgtaga
     1021 tatgcaacca gatccaaacg tgacttcatg tacagaaaac gtttctgcag caaaatttga
     1081 ctacaagcta aacaatattt tcagacttca tgaactccca gtgagctgca tcgtcatgca
     1141 tccctgcaga gactatctca tctcctgtag tgaagatcgt ctctggaaga tggtgggcct
     1201 cccacagggc aatgtacttc ttacaggatc tggccacact gattggttgt ctggctgctg
     1261 cttccatccg agtggcagca agttggctac ctcaagtggt gacagcacaa tcaaactatg
     1321 ggacctcaac aaaggtgaat gcaccttgac ccttgaagga cacaaccatg ccgtatggtc
     1381 ctgcacctgg cactcctgtg gtgacttcgt ggcttctgcc tcactggata tgacgagtaa
     1441 aatctgggat gtcaacagtg aacggtgcag atacactttg tatggacaca cagactctgt
     1501 gaacagcatt gagtttttcc ctttctccaa cattcttctc accgcttctg cagacaagac
     1561 cttgtctgtg tgggatgcac gaacaggtaa atgtgagcag tctctgtatg gtcacatgca
     1621 ttctgtcaat gatgccacct tcacgcctag gggtcacata atagcatcct gtgatgcccg
     1681 tggagttaca aagctgtggg acttccggaa actcattcca atcgtgtcca ttgatgtggg
     1741 tccaagttcg ggaaatgagg tgaactttga ccagtcaggt cgagttttag ctcaggcgag
     1801 tgccaacggg attatccatt tgctggatct taaatcgggg caaattcaca aactggtggg
     1861 ccacgagagt gaagtgcaca gtgtggtgtt ttcccacctg ggggaaaatc tgtattctgg
     1921 aggctctgac ggcaccattc ggttgtggat ctgatcagca atagcacacc ctgatgcaaa
     1981 gggcattccc tctaaggctt aaaaatgttt catatagtct gctcaaatgc cagcaagtaa
     2041 ctggttaaaa tgaccaataa ataaaatatt tgttttaaaa caagctttgg gtgaataata
     2101 tatggaccat actatctcaa ataaaaatta taactttttg ttca
//
