LOCUS       NM_153115                435 bp    mRNA    linear   ROD 10-DEC-2024
DEFINITION  Mus musculus sperm associated antigen 11A (Spag11a), mRNA.
ACCESSION   NM_153115
VERSION     NM_153115.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Sangeeta,K., Aisha,J. and Yenugu,S.
  TITLE     The Sperm-Associated Antigen 11A (Spag11a) Knockout Mice Display
            Sub-Fertility and Perturbations in the Sperm Proteome
  JOURNAL   Cell Biochem Funct 42 (7), e70003 (2024)
   PUBMED   39425448
REFERENCE   2  (bases 1 to 435)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 435)
  AUTHORS   Pujianto,D.A., Loanda,E., Sari,P., Midoen,Y.H. and Soeharso,P.
  TITLE     Sperm-associated antigen 11A is expressed exclusively in the
            principal cells of the mouse caput epididymis in an
            androgen-dependent manner
  JOURNAL   Reprod Biol Endocrinol 11, 59 (2013)
   PUBMED   23815807
  REMARK    GeneRIF: Data suggest that Spag11a has a specific role in
            epididymal sperm maturation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 435)
  AUTHORS   Fei,Z., Hu,S., Xiao,L., Zhou,J., Diao,H., Yu,H., Fang,S., Wang,Y.,
            Wan,Y., Wang,W., He,Y., Wang,C., Xu,G., Wang,Z., Zhang,Y. and
            Fei,J.
  TITLE     mBin1b transgenic mice show enhanced resistance to epididymal
            infection by bacteria challenge
  JOURNAL   Genes Immun 13 (6), 445-451 (2012)
   PUBMED   22535201
  REMARK    GeneRIF: Model was developed in which Bin1b was exclusively
            overexpressed by up to 50-fold in the caput epididymis. The
            transgenic mice are healthy with normal reproductive activity, but
            are more resistant to epididymal infection from E coli than normal
            animals.
REFERENCE   5  (bases 1 to 435)
  AUTHORS   Biswas,B. and Yenugu,S.
  TITLE     Antimicrobial responses in the male reproductive tract of
            lipopolysaccharide challenged rats
  JOURNAL   Am J Reprod Immunol 65 (6), 557-568 (2011)
   PUBMED   21199063
  REMARK    GeneRIF: increased expression in response to lipopolysaccharide
            administration
REFERENCE   6  (bases 1 to 435)
  AUTHORS   Amid,C., Rehaume,L.M., Brown,K.L., Gilbert,J.G., Dougan,G.,
            Hancock,R.E. and Harrow,J.L.
  TITLE     Manual annotation and analysis of the defensin gene cluster in the
            C57BL/6J mouse reference genome
  JOURNAL   BMC Genomics 10, 606 (2009)
   PUBMED   20003482
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 435)
  AUTHORS   Patil,A.A., Cai,Y., Sang,Y., Blecha,F. and Zhang,G.
  TITLE     Cross-species analysis of the mammalian beta-defensin gene family:
            presence of syntenic gene clusters and preferential expression in
            the male reproductive tract
  JOURNAL   Physiol Genomics 23 (1), 5-17 (2005)
   PUBMED   16033865
REFERENCE   8  (bases 1 to 435)
  AUTHORS   Yamaguchi,Y., Nagase,T., Makita,R., Fukuhara,S., Tomita,T.,
            Tominaga,T., Kurihara,H. and Ouchi,Y.
  TITLE     Identification of multiple novel epididymis-specific beta-defensin
            isoforms in humans and mice
  JOURNAL   J Immunol 169 (5), 2516-2523 (2002)
   PUBMED   12193721
  REMARK    GeneRIF: The epididymis-specific expression pattern of the
            beta-defensin isoform EP2e has been characterized.
REFERENCE   9  (bases 1 to 435)
  AUTHORS   Ibrahim,N.M., Young,L.G. and Frohlich,O.
  TITLE     Epididymal specificity and androgen regulation of rat EP2
  JOURNAL   Biol Reprod 65 (2), 575-580 (2001)
   PUBMED   11466228
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AB089183.1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB089183.1, AK020333.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849376, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 10.35 cM"
     gene            1..435
                     /gene="Spag11a"
                     /gene_synonym="9230111C08Rik; Bin1b; EP2e; EP2Q; Spag11"
                     /note="sperm associated antigen 11A"
                     /db_xref="GeneID:78128"
                     /db_xref="MGI:MGI:1925378"
     exon            1..113
                     /gene="Spag11a"
                     /gene_synonym="9230111C08Rik; Bin1b; EP2e; EP2Q; Spag11"
                     /inference="alignment:Splign:2.1.0"
     CDS             59..268
                     /gene="Spag11a"
                     /gene_synonym="9230111C08Rik; Bin1b; EP2e; EP2Q; Spag11"
                     /note="sperm-associated antigen 11; beta defensin EP2e;
                     antimicrobial peptide Bin1b; EP2 protein;
                     antimicrobial-like protein Bin-1b"
                     /codon_start=1
                     /product="sperm-associated antigen 11A precursor"
                     /protein_id="NP_694755.1"
                     /db_xref="CCDS:CCDS40252.1"
                     /db_xref="GeneID:78128"
                     /db_xref="MGI:MGI:1925378"
                     /translation="MKVLLLFAVFFCFVQGNSGDIPPGIRNTVCLMQQGHCRLFMCRS
                     GERKGDICSDPWNRCCVPYSVKDRR"
     exon            114..435
                     /gene="Spag11a"
                     /gene_synonym="9230111C08Rik; Bin1b; EP2e; EP2Q; Spag11"
                     /inference="alignment:Splign:2.1.0"
     regulatory      375..380
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag11a"
                     /gene_synonym="9230111C08Rik; Bin1b; EP2e; EP2Q; Spag11"
                     /note="hexamer: AATAAA"
     polyA_site      399
                     /gene="Spag11a"
                     /gene_synonym="9230111C08Rik; Bin1b; EP2e; EP2Q; Spag11"
                     /note="major polyA site"
ORIGIN      
        1 ggattaactc agtcatcagt cacacctgct ttcctgcaca gagagcgagc cgtaaaacat
       61 gaaagtcttg ttactctttg ctgtcttctt ctgctttgtc caaggaaact caggggacat
      121 cccacccgga atcagaaaca ccgtgtgcct catgcaacag ggccactgca ggctcttcat
      181 gtgccgttct ggtgagagaa aaggggatat ctgctccgac ccctggaaca gatgctgcgt
      241 cccctattct gttaaagaca gaagatagaa gactgaggtt gggagatctg gaatgtgggg
      301 cgcaagcaac tgaagggtgg agacttggaa atgccccaaa ggctttgagt atgtgtggct
      361 gacggtgcat actcaataaa catttgctaa ataaatctat caccgaggca aaggtccggt
      421 gaaaggattt tatgg
//
