LOCUS       NM_001359982            4032 bp    mRNA    linear   ROD 06-JUN-2024
DEFINITION  Mus musculus sperm associated antigen 1 (Spag1), transcript variant
            5, mRNA.
ACCESSION   NM_001359982 XM_017316632
VERSION     NM_001359982.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4032)
  AUTHORS   Huang,C., Wu,D., Khan,F.A., Jiao,X., Guan,K. and Huo,L.
  TITLE     The GTPase SPAG-1 orchestrates meiotic program by dictating meiotic
            resumption and cytoskeleton architecture in mouse oocytes
  JOURNAL   Mol Biol Cell 27 (11), 1776-1785 (2016)
   PUBMED   27053660
  REMARK    GeneRIF: Findings broaden our knowledge of SPAG-1, showing that it
            exerts a role in oocyte meiotic execution via its involvement in
            AMPK and MAPK signaling pathways.
REFERENCE   2  (bases 1 to 4032)
  AUTHORS   Hayashida,K., Omagari,K., Masuda,J. and Kohno,S.
  TITLE     An integrase of endogenous retrovirus is involved in maternal
            mitochondrial DNA inheritance of the mouse
  JOURNAL   Biochem Biophys Res Commun 366 (1), 206-211 (2008)
   PUBMED   18054325
REFERENCE   3  (bases 1 to 4032)
  AUTHORS   Hayashida,K., Omagari,K., Masuda,J., Hazama,H., Kadokawa,Y.,
            Ohba,K. and Kohno,S.
  TITLE     The sperm mitochondria-specific translocator has a key role in
            maternal mitochondrial inheritance
  JOURNAL   Cell Biol Int 29 (6), 472-481 (2005)
   PUBMED   15979907
  REMARK    GeneRIF: The number of PCR positives of sperm mtDNA in two-cell
            embryos was significantly increased following sperm incubation with
            anti-tetratricopeptide repeat-containing protein involved in
            spermatogenesis (tpis).
REFERENCE   4  (bases 1 to 4032)
  AUTHORS   Yamashita,H., Avraham,S., Jiang,S., London,R., Van Veldhoven,P.P.,
            Subramani,S., Rogers,R.A. and Avraham,H.
  TITLE     Characterization of human and murine PMP20 peroxisomal proteins
            that exhibit antioxidant activity in vitro
  JOURNAL   J Biol Chem 274 (42), 29897-29904 (1999)
   PUBMED   10514471
REFERENCE   5  (bases 1 to 4032)
  AUTHORS   Takaishi,M. and Huh,Nh.
  TITLE     A tetratricopeptide repeat-containing protein gene, tpis, whose
            expression is induced with differentiation of spermatogenic cells
  JOURNAL   Biochem Biophys Res Commun 264 (1), 81-85 (1999)
   PUBMED   10527845
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC121577.3.
            
            On Jan 24, 2018 this sequence version replaced XM_017316632.1.
            
            Transcript Variant: This variant (5) differs in the 5' UTR compared
            to variant 1. Variants 1, 5 and 6 encode the same protein.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR6116558.109245.1,
                                           SRR1660819.75097.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-448               AC121577.3         83284-83731         c
            449-601             AC121577.3         81882-82034         c
            602-749             AC121577.3         78642-78789         c
            750-909             AC121577.3         76669-76828         c
            910-1035            AC121577.3         73753-73878         c
            1036-1100           AC121577.3         69949-70013         c
            1101-1210           AC121577.3         69250-69359         c
            1211-1316           AC121577.3         68376-68481         c
            1317-1447           AC121577.3         64979-65109         c
            1448-1554           AC121577.3         62641-62747         c
            1555-1714           AC121577.3         60124-60283         c
            1715-1993           AC121577.3         49664-49942         c
            1994-2093           AC121577.3         49286-49385         c
            2094-2246           AC121577.3         44166-44318         c
            2247-2407           AC121577.3         38919-39079         c
            2408-2540           AC121577.3         36244-36376         c
            2541-2667           AC121577.3         33223-33349         c
            2668-2808           AC121577.3         27142-27282         c
            2809-3177           AC121577.3         25816-26184         c
            3178-4032           AC121577.3         24864-25718         c
FEATURES             Location/Qualifiers
     source          1..4032
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 14.46 cM"
     gene            1..4032
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /note="sperm associated antigen 1"
                     /db_xref="GeneID:26942"
                     /db_xref="MGI:MGI:1349387"
     exon            1..448
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            449..601
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     CDS             568..3309
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /note="isoform 1 is encoded by transcript variant 5;
                     infertility-related sperm protein Spag-1; TPR-containing
                     protein involved in spermatogenesis"
                     /codon_start=1
                     /product="sperm-associated antigen 1 isoform 1"
                     /protein_id="NP_001346911.1"
                     /db_xref="GeneID:26942"
                     /db_xref="MGI:MGI:1349387"
                     /translation="MQFSILQHETLTMTAKAKDCPSLWGFGTTKTFKIPIEHLDFKYI
                     ENCSDVKHLEKILYVLRSGEEGYYPELTEFCEKCLTNLAPKSRALRKDKPAETASSFS
                     AEEWEKIDSDLKSWVSEIKREENTCHFHDPENHPGVEDPLPPVRGSTCCPHSGKETYS
                     KSKTAKKRIPRDYAEWDKFDVEKECSKIDEDYKEKTVINNKAHLSKIETKIETAGLTE
                     KEKSFLANREKGKGNEAFYSGDYEEAVMYYTRSLSALPTAIAYNNRAQAEIKLQRWSS
                     ALEDCEKALELDPGNVKALLRRATTYKHQNKLQEAVDDLRKVLQVEPDNDLAKKTLSE
                     VERDLKNSEPVSELQTKGKRMVIEEVENSGDEGGKGSADEREDGGSDEAAMGNIQKKL
                     MVRRSEGGRRSRRGRTPGPRAEQQGGLRETATASTGDSHYPEEPRAADNPSGLKRRGN
                     ELFRGGQFAEAAAQYSVAIAQLEPTGSANADELSILYSNRAACYLKEGNCRDCIQDCN
                     RALELHPFSVKPLLRRAMAYETLEQYRNAYVDYKTVLQIDCGIQLASDSANRIARILT
                     ELDGSKWRERLPPIPAVPTSEPLRVWLPAAETPDQDPCPNNCMPSITDEKMFQALKEE
                     GNQLVKDKNYKDAISKYNECLKINSKACAIYTNRALCYLKLGQFEEAKLDCEQALQID
                     GENVKASHRLALAQKGLENCRESGVDPSQVLLSPDSSEAARHLDTKNDTAPPSKGRER
                     RRIQVQEVDGSSDEEPERPAEASATSAPARDGVEDGGSAEPAEKLDVSKPTNAYEFGQ
                     VLSTISARKDEEACAHLLAITAPKDLPLLLSNKLEGDTFLLLIQSLKSHLVAKDPSLV
                     YEHLLYLSKAERFKTMLTLINKGQKEQMAQLFDGLSDTQSDGLTAEDVQALRRQYEL"
     exon            602..749
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            750..909
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            910..1035
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            1036..1100
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            1101..1210
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            1211..1316
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            1317..1447
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            1448..1554
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            1555..1714
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            1715..1993
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            1994..2093
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2246
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            2247..2407
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2540
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            2541..2667
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            2668..2808
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            2809..3177
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     exon            3178..4032
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3570..3575
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /note="hexamer: ATTAAA"
     polyA_site      3588
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /note="major polyA site"
     regulatory      3854..3859
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /note="hexamer: AATAAA"
     polyA_site      3874
                     /gene="Spag1"
                     /gene_synonym="tpis"
     regulatory      4003..4008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /note="hexamer: AATAAA"
     polyA_site      4032
                     /gene="Spag1"
                     /gene_synonym="tpis"
                     /note="major polyA site"
ORIGIN      
        1 tggtggtttt tgatgctcga tgaaatgtgg gagttcagaa gtatcttcat tcatacattt
       61 gcctcattga tgtttctctt taatgtatag catttggctt agtttactta atgaaaacaa
      121 ctgtatgcct atgatttcat ttagaaatac aattgtattt taatactttg tataaaggaa
      181 attttggttc aatatatggc ttaatttatt gcttttgtaa tccgtcacac agtgaagatt
      241 aaaagtgttt cctcaaacca tttctattca atgtcttgtt agaaagccat caagccagat
      301 gacttgcttc aggccctggg atccacgtag tggaagagga atggactcct gaaatgggtc
      361 tgtcctctga cctagacttg ggcatgtgca tgtagaacac ccacagagaa gaagaactaa
      421 aatagaacat tgaaaaaggt ctgaagaaga aatcagtgct tcggaagttc aggtgtttgc
      481 ttgagactcg ctttgctgtc tggatgctgt tcacataagt gagacagctt aaggattatc
      541 cgaaagattg agtcttcatc tataaatatg cagtttagca ttctccaaca tgaaacccta
      601 accatgactg ctaaggctaa ggactgtcca tcattgtggg gctttggaac aacaaaaaca
      661 tttaaaattc ccatcgaaca tttggatttc aagtatattg aaaactgttc agatgtgaaa
      721 cacctggaaa aaattcttta tgtgctgagg tctggtgaag aaggctacta tcctgaactc
      781 acggaatttt gcgagaagtg cctcaccaac ttggctccta aaagtcgagc tctgaggaag
      841 gacaagcctg cagagacagc atccagcttt tcagcggaag agtgggaaaa aatcgacagt
      901 gatctaaaga gttgggtgtc agaaatcaaa agagaggaaa atacatgtca cttccatgac
      961 cctgaaaacc atcccggagt ggaagatcct ctgcctcctg tccgcggctc cacctgctgc
     1021 cctcacagtg gcaaggaaac atattctaaa agtaagacag ctaagaagag gattccaagg
     1081 gattatgcag aatgggataa atttgatgtg gaaaaagaat gttcaaagat tgatgaggat
     1141 tacaaagaaa agactgtcat aaacaacaag gctcatctgt ctaaaattga gacaaaaata
     1201 gaaacagcag gtctgactga gaaggaaaag agttttcttg ccaatcgtga aaagggaaaa
     1261 gggaacgagg ctttctactc tggggactat gaagaggccg tgatgtatta caccaggagc
     1321 ttatcagccc ttcccaccgc cattgcctat aacaatcgag ctcaggccga aatcaagttg
     1381 cagagatgga gcagtgctct tgaagactgt gagaaggcct tggagctaga tcccggaaac
     1441 gtaaaagctc tcctgagacg agccactaca tataaacatc aaaacaagct ccaggaagct
     1501 gtggacgacc tgagaaaggt gctgcaggtg gagccggaca acgacttggc caagaagacc
     1561 ttatcagagg ttgaaagaga tctgaagaat tcagagcctg tctctgagct ccaaaccaaa
     1621 gggaagagga tggttattga agaagttgaa aactcgggag atgaaggtgg aaagggaagc
     1681 gcagatgagc gtgaagatgg tggctcggat gaggcggcca tgggcaacat tcagaagaag
     1741 ctgatggtcc gccggagcga gggtggcagg cggtcgcggc ggggccggac accggggccc
     1801 cgagctgagc agcagggagg cctgcgagag acagcgacgg ccagcaccgg cgacagtcac
     1861 tacccggagg agccgcgagc cgctgacaac cccagcggcc tcaagagacg gggcaacgag
     1921 ctgttccgcg gcgggcaatt cgcagaggca gccgcgcaat actcggtggc gatagcgcag
     1981 ctggaaccca caggaagtgc aaatgcggat gagctaagca tcttgtattc aaatagagcg
     2041 gcatgctacc tcaaagaagg gaactgcagg gactgcatcc aggattgtaa cagggctctg
     2101 gaacttcatc cgttctcagt gaagcctctt ctgcggcgag caatggccta tgagacctta
     2161 gagcaataca ggaacgcata tgtggactat aaaacagtgc tgcagataga ttgtgggatc
     2221 cagctggcga gcgacagtgc taacagaata gcacgaatct taacagagct agatggatca
     2281 aaatggcggg agagactgcc acccatccca gctgtaccca catctgagcc actgcgagtt
     2341 tggctccctg ctgcagagac cccagaccaa gatccctgtc ccaacaactg catgccgagc
     2401 atcacagatg aaaaaatgtt tcaagccctc aaggaagaag gaaatcaact tgtaaaggac
     2461 aaaaattata aagatgccat tagtaaatac aatgaatgct taaagattaa cagcaaggca
     2521 tgtgccatct atacaaacag ggctctctgt tacctgaagc tgggccagtt tgaagaggca
     2581 aagctggact gtgagcaggc gcttcagata gacggcgaga atgtgaaagc cagccacaga
     2641 ctagcgctcg cccagaaagg actcgagaac tgccgggaaa gcggggtgga ccccagtcaa
     2701 gtcctcctaa gcccagattc cagtgaagcc gcccggcacc tggacactaa gaatgacaca
     2761 gcacctccca gcaaaggaag ggagaggagg agaatccaag tccaagaggt ggatggcagc
     2821 agtgatgagg agcctgagag acctgcagag gcctctgcta cctccgcacc tgccagagat
     2881 ggtgtggaag atggagggtc tgcagaacca gcagaaaaac tcgacgtctc caagcctact
     2941 aatgcctatg agtttggaca ggtcctaagc actatcagtg ccaggaaaga tgaagaggcc
     3001 tgtgcacatc ttttagccat cactgcaccc aaagacctgc cactgttgct aagtaacaaa
     3061 cttgaggggg acacgttcct cctcctcatt cagtcactta aaagtcacct tgtggctaaa
     3121 gacccctccc tggtgtatga gcatctcctg tatctgagca aagcagagag gtttaagacg
     3181 atgctgacac taattaacaa aggccaaaag gagcagatgg cgcagctgtt tgatggtctg
     3241 tcagacacac agtccgacgg tttgacggca gaggatgtac aggccctaag aaggcagtat
     3301 gagctttaac ttggggttgc tgagttcaag acagcaaaca ttgtgatgca gttgcatggg
     3361 taaagccaca cccagaagga tccctgtttg gacatcagat gttgtactta tttttatatc
     3421 tagaacatgt atattctata atctgatttt tatttattgt aaatattctc ttctgtacta
     3481 gagccaacac ctttatattt aataactata ttcagaactt ggcaaattat actctagctt
     3541 ataatacatt ttcttttaat gacctaccca ttaaaaattt aaaatacatt ctttgtggtg
     3601 gcagttcttt caggtttgac agcaatcagt gactttagtc ctttaaaagc aaatgttagc
     3661 agttgcgact ccatcacttt ctgtcccact caaatgcaca aaatccactt tagagaagtg
     3721 gcttactgtc acttgagatg aaattcaaca cttaatgtct atgagatcat tattagttta
     3781 ttaatttaga gccctaaagg agttactttt tctaatattt agtgtgactt tgtcttctac
     3841 attgcttgta agcaataaaa ctgtatgaaa caaattcacc ctgcttttgt atttgaattg
     3901 tcccccaccc caactttttg ttttctgctt tatttcttta ctctaacaag tggaaaaagt
     3961 cactggatac tcctttttct gtgttaaagg tattttctac acaataaaca ttcaagcctt
     4021 tatttactgt ga
//
