LOCUS       NM_027283                901 bp    mRNA    linear   ROD 20-SEP-2024
DEFINITION  Mus musculus sperm acrosome associated 9 (Spaca9), mRNA.
ACCESSION   NM_027283 XM_130129
VERSION     NM_027283.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 901)
  AUTHORS   Tai,L., Yin,G., Huang,X., Sun,F. and Zhu,Y.
  TITLE     In-cell structural insight into the stability of sperm microtubule
            doublet
  JOURNAL   Cell Discov 9 (1), 116 (2023)
   PUBMED   37989994
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 901)
  AUTHORS   Chen,Z., Shiozaki,M., Haas,K.M., Skinner,W.M., Zhao,S., Guo,C.,
            Polacco,B.J., Yu,Z., Krogan,N.J., Lishko,P.V., Kaake,R.M.,
            Vale,R.D. and Agard,D.A.
  TITLE     De novo protein identification in mammalian sperm using in situ
            cryoelectron tomography and AlphaFold2 docking
  JOURNAL   Cell 186 (23), 5041-5053 (2023)
   PUBMED   37865089
REFERENCE   4  (bases 1 to 901)
  AUTHORS   Castaneda,J.M., Shimada,K., Satouh,Y., Yu,Z., Devlin,D.J., Ikawa,M.
            and Matzuk,M.M.
  TITLE     FAM209 associates with DPY19L2, and is required for sperm acrosome
            biogenesis and fertility in mice
  JOURNAL   J Cell Sci 134 (21) (2021)
   PUBMED   34471926
REFERENCE   5  (bases 1 to 901)
  AUTHORS   Stauber,M., Weidemann,M., Dittrich-Breiholz,O., Lobschat,K.,
            Alten,L., Mai,M., Beckers,A., Kracht,M. and Gossler,A.
  TITLE     Identification of FOXJ1 effectors during ciliogenesis in the foetal
            respiratory epithelium and embryonic left-right organiser of the
            mouse
  JOURNAL   Dev Biol 423 (2), 170-188 (2017)
   PUBMED   27914912
REFERENCE   6  (bases 1 to 901)
  AUTHORS   Bhattacharya,R., Devi,M.S., Dhople,V.M. and Jesudasan,R.A.
  TITLE     A mouse protein that localizes to acrosome and sperm tail is
            regulated by Y-chromosome
  JOURNAL   BMC Cell Biol 14, 50 (2013)
   PUBMED   24256100
  REMARK    GeneRIF: identified a mouse acrosomal protein, MAST, that is
            expressed testis specifically. MAST does not contain any known
            motifs for protein interactions; yet it complexes with
            calcium-binding proteins localizing to the acrosome.
            Publication Status: Online-Only
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC055108.1.
            
            On Jan 24, 2005 this sequence version replaced XM_130129.4.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC055108.1, CN842740.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384, SAMN00849388
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 19.38 cM"
     gene            1..901
                     /gene="Spaca9"
                     /gene_synonym="1700026L06Rik; MAST"
                     /note="sperm acrosome associated 9"
                     /db_xref="GeneID:69987"
                     /db_xref="MGI:MGI:1917237"
     exon            1..70
                     /gene="Spaca9"
                     /gene_synonym="1700026L06Rik; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            71..247
                     /gene="Spaca9"
                     /gene_synonym="1700026L06Rik; MAST"
                     /inference="alignment:Splign:2.1.0"
     CDS             104..610
                     /gene="Spaca9"
                     /gene_synonym="1700026L06Rik; MAST"
                     /note="acrosome and sperm tail protein"
                     /codon_start=1
                     /product="sperm acrosome-associated protein 9"
                     /protein_id="NP_081559.1"
                     /db_xref="CCDS:CCDS15845.1"
                     /db_xref="GeneID:69987"
                     /db_xref="MGI:MGI:1917237"
                     /translation="MNEVKESLRSIEQKYKLFQQQQFTFIAALEHCRENAHDKIRPIS
                     SIEQVQSYMEHYCNNSTHRRILIMFMDICSELSKLCQHFEALHSGTPVTNSLLEKCKT
                     LVSQSNDLSSLRAKYPHEVVNHLSCDEARNHYGGVVSLIPIVLDFMKEWIAHSEKLPR
                     KVLQHGTT"
     exon            248..450
                     /gene="Spaca9"
                     /gene_synonym="1700026L06Rik; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            451..598
                     /gene="Spaca9"
                     /gene_synonym="1700026L06Rik; MAST"
                     /inference="alignment:Splign:2.1.0"
     exon            599..671
                     /gene="Spaca9"
                     /gene_synonym="1700026L06Rik; MAST"
                     /inference="alignment:Splign:2.1.0"
     regulatory      649..654
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spaca9"
                     /gene_synonym="1700026L06Rik; MAST"
                     /note="hexamer: AATAAA"
     polyA_site      671
                     /gene="Spaca9"
                     /gene_synonym="1700026L06Rik; MAST"
                     /note="major polyA site"
ORIGIN      
        1 gcacacccac ggaggcactg caagcttctg ggcactctgt gaggcgaagg ccagtccaag
       61 tcaaaccagg accctttctg tcctgtgaga cctaagagca acaatgaatg aggtgaaaga
      121 gtccctccgc agcatcgagc agaagtataa gctcttccag cagcaacagt tcaccttcat
      181 tgcggctctg gagcactgcc gggagaatgc ccacgacaaa atccggccca tctccagtat
      241 cgagcaggtg cagagctaca tggaacacta ctgcaataac tccacccacc ggcggattct
      301 gatcatgttc atggacatct gctccgagct cagcaagctc tgtcagcact ttgaggccct
      361 gcactctggc acccctgtca ccaacagcct tctcgagaaa tgtaaaaccc tggtcagcca
      421 aagcaatgac ttgagcagcc tcagagccaa gtatccgcac gaagtggtta accacctcag
      481 ctgcgatgag gccaggaacc actatggagg cgtggtcagc ctcatcccta tagtcctgga
      541 ctttatgaaa gagtggattg ctcattctga gaagctgcct cgcaaagtgc tgcagcatgg
      601 gacgacatag cttctgtact gttgcttcct cagggggaga cattcaagaa taaagtgttc
      661 taccacttct caaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      721 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      781 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      841 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      901 a
//
