LOCUS       NM_024279                778 bp    mRNA    linear   ROD 12-JUN-2024
DEFINITION  Mus musculus sperm acrosome associated 7 (Spaca7), transcript
            variant 1, mRNA.
ACCESSION   NM_024279
VERSION     NM_024279.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Mrowiec,K., Chadalski,M., Paszek,A., Vydra,N., Janus,P.,
            Pietrowska,M., Toma-Jonik,A., Gramatyka,M. and Widlak,W.
  TITLE     The periphilin 1-like BFAR isoform 3 is highly expressed in
            transcriptionally silent oocytes and involved in RNA metabolism
  JOURNAL   Biochim Biophys Acta Mol Cell Res 1868 (10), 119086 (2021)
   PUBMED   34175335
REFERENCE   2  (bases 1 to 778)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   3  (bases 1 to 778)
  AUTHORS   Nguyen,E.B., Westmuckett,A.D. and Moore,K.L.
  TITLE     SPACA7 is a novel male germ cell-specific protein localized to the
            sperm acrosome that is involved in fertilization in mice
  JOURNAL   Biol Reprod 90 (1), 16 (2014)
   PUBMED   24307706
  REMARK    GeneRIF: Release of SPACA7 from the acrosome accelerates cumulus
            dispersal and facilitates fertilization via unknown mechanisms.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 778)
  AUTHORS   Korfanty,J., Toma,A., Wojtas,A., Rusin,A., Vydra,N. and Widlak,W.
  TITLE     Identification of a new mouse sperm acrosome-associated protein
  JOURNAL   Reproduction 143 (6), 749-757 (2012)
   PUBMED   22495889
  REMARK    GeneRIF: Confocal microscopy studies revealed an intra-acrosomal
            not membrane-bound localization of SPACA7/EGFP, which suggests that
            the protein can be released during acrosome reaction and involved
            in fertilization.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC161505.4.
            
            On Jan 5, 2022 this sequence version replaced NM_024279.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK018926.1, BY707127.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-162               AC161505.4         86047-86208         c
            163-219             AC161505.4         78246-78302         c
            220-327             AC161505.4         73502-73609         c
            328-432             AC161505.4         72707-72811         c
            433-504             AC161505.4         69970-70041         c
            505-603             AC161505.4         60204-60302         c
            604-778             AC161505.4         58502-58676         c
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 5.73 cM"
     gene            1..778
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /note="sperm acrosome associated 7"
                     /db_xref="GeneID:78634"
                     /db_xref="MGI:MGI:1925884"
     exon            1..162
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             69..617
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; protein SPACA7; sperm acrosome-associated
                     protein 7"
                     /codon_start=1
                     /product="sperm acrosome-associated protein 7 isoform 1
                     precursor"
                     /protein_id="NP_077241.1"
                     /db_xref="CCDS:CCDS40222.1"
                     /db_xref="GeneID:78634"
                     /db_xref="MGI:MGI:1925884"
                     /translation="MAANRGSRTFLSVFLLCCWQGAELQPIKTTSGPITEGSLNSTTE
                     NIPEALDEILAQEILEPKTSAVSETSPRPRSSILTTVQTKEINAGIDENYQEEAFENY
                     HEVLENIEHLPTKEESGKNDRSTVANLHDHSSQTKHEPPSSPEGKGSSNDDVYGKLSV
                     LDKILENIGQSEGSLELTESIF"
     exon            163..219
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            220..327
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            328..432
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            433..504
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            505..603
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            604..778
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      755..760
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /note="hexamer: AAGAAA"
     polyA_site      778
                     /gene="Spaca7"
                     /gene_synonym="1700094C09Rik"
                     /note="major polyA site"
ORIGIN      
        1 agtccaaggc tggggaagct gaagacctac cttccaggga agggcctcct cctgtaactg
       61 gagggaccat ggcagcgaat agaggatcga ggaccttctt atctgtcttc ctgctgtgct
      121 gctggcaagg cgctgagctg cagccgatca agacaacttc aggtccaatt actgaaggtt
      181 cactcaactc aacaacagaa aacatacccg aagcattaga tgaaattcta gcccaagaga
      241 ttttggagcc caaaacatca gcagtgtctg aaacatcacc acgaccaaga tcatcgatac
      301 tgacaacagt acaaaccaag gaaataaatg ctggtattga tgaaaactac caagaggaag
      361 cctttgagaa ctaccatgag gtcctggaga acatagaaca cttgcctaca aaggaggaaa
      421 gtggcaaaaa tgacagaagc acggttgcaa accttcatga ccacagctct cagactaagc
      481 atgagccacc ctcgtctcct gaaggaaagg ggagttcaaa cgatgatgtg tatgggaaac
      541 tgtctgttct ggacaaaatc cttgaaaaca taggacaatc tgaaggcagc ctggagctaa
      601 cagaaagcat cttttaggag gagaagaaga ggaccaagcc caggaggaca gcgagacaca
      661 ggcgctgctg agcttagaaa ggcaaggagc caccggagag ctccatgcca cccatcttag
      721 ggaatgcaca cactcactgt tactggttct taaaaagaaa attgtttttt ccatgtta
//
