LOCUS       NM_001162909            1102 bp    mRNA    linear   ROD 06-AUG-2024
DEFINITION  Mus musculus sperm acrosome associated 6 (Spaca6), mRNA.
ACCESSION   NM_001162909
VERSION     NM_001162909.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Kaurani,L., Islam,M.R., Heilbronner,U., Kruger,D.M., Zhou,J.,
            Methi,A., Strauss,J., Pradhan,R., Schroder,S., Burkhardt,S.,
            Schuetz,A.L., Pena,T., Erlebach,L., Buhler,A., Budde,M., Senner,F.,
            Kohshour,M.O., Schulte,E.C., Schmauss,M., Reininghaus,E.Z.,
            Juckel,G., Kronenberg-Versteeg,D., Delalle,I., Odoardi,F.,
            Flugel,A., Schulze,T.G., Falkai,P., Sananbenesi,F. and Fischer,A.
  TITLE     Regulation of Zbp1 by miR-99b-5p in microglia controls the
            development of schizophrenia-like symptoms in mice
  JOURNAL   EMBO J 43 (8), 1420-1444 (2024)
   PUBMED   38528182
  REMARK    GeneRIF: Regulation of Zbp1 by miR-99b-5p in microglia controls the
            development of schizophrenia-like symptoms in mice.
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   Matsumura,T., Noda,T., Satouh,Y., Morohoshi,A., Yuri,S., Ogawa,M.,
            Lu,Y., Isotani,A. and Ikawa,M.
  TITLE     Sperm IZUMO1 Is Required for Binding Preceding Fusion With Oolemma
            in Mice and Rats
  JOURNAL   Front Cell Dev Biol 9, 810118 (2022)
   PUBMED   35096839
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1102)
  AUTHORS   Bhatlekar,S., Jacob,S., Manne,B.K., Guo,L., Denorme,F.,
            Tugolukova,E.A., Cody,M.J., Kosaka,Y., Rigoutsos,I., Campbell,R.A.,
            Rowley,J.W., O'Connell,R.M. and Bray,P.F.
  TITLE     Megakaryocyte-specific knockout of the Mir-99b/let7e/125a cluster
            lowers platelet count without altering platelet function
  JOURNAL   Blood Cells Mol Dis 92, 102624 (2021)
   PUBMED   34775219
  REMARK    GeneRIF: Megakaryocyte-specific knockout of the Mir-99b/let7e/125a
            cluster lowers platelet count without altering platelet function.
REFERENCE   4  (bases 1 to 1102)
  AUTHORS   Inoue,N., Hagihara,Y. and Wada,I.
  TITLE     Evolutionarily conserved sperm factors, DCST1 and DCST2, are
            required for gamete fusion
  JOURNAL   Elife 10, e66313 (2021)
   PUBMED   33871360
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1102)
  AUTHORS   Xu,Y., Chen,X., Zhao,C., Wang,X., Cheng,Y., Xi,F., Yao,X.,
            Zhang,L., Yang,G. and Yu,T.
  TITLE     MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and Lpin1 in
            3T3-L1 Cells
  JOURNAL   J Agric Food Chem 69 (8), 2564-2575 (2021)
   PUBMED   33599498
  REMARK    GeneRIF: MiR-99b-5p Attenuates Adipogenesis by Targeting SCD1 and
            Lpin1 in 3T3-L1 Cells.
REFERENCE   6  (bases 1 to 1102)
  AUTHORS   Barbaux,S., Ialy-Radio,C., Chalbi,M., Dybal,E., Homps-Legrand,M.,
            Do Cruzeiro,M., Vaiman,D., Wolf,J.P. and Ziyyat,A.
  TITLE     Sperm SPACA6 protein is required for mammalian Sperm-Egg
            Adhesion/Fusion
  JOURNAL   Sci Rep 10 (1), 5335 (2020)
   PUBMED   32210282
  REMARK    GeneRIF: Sperm SPACA6 protein is required for mammalian Sperm-Egg
            Adhesion/Fusion.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 1102)
  AUTHORS   Grossman,H., Har-Paz,E., Gindi,N., Levi,M., Miller,I., Nevo,N.,
            Galiani,D., Dekel,N. and Shalgi,R.
  TITLE     Regulation of GVBD in mouse oocytes by miR-125a-3p and Fyn kinase
            through modulation of actin filaments
  JOURNAL   Sci Rep 7 (1), 2238 (2017)
   PUBMED   28533542
  REMARK    GeneRIF: Our findings suggest a mechanism, by which a decrease in
            miR-125a-3p during oocyte maturation facilitates GVBD by allowing
            Fyn up-regulation and the resulting stabilization of the
            interaction between actin and A-type lamins.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 1102)
  AUTHORS   Che,P., Liu,J., Shan,Z., Wu,R., Yao,C., Cui,J., Zhu,X., Wang,J.,
            Burnett,M.S., Wang,S. and Wang,J.
  TITLE     miR-125a-5p impairs endothelial cell angiogenesis in aging mice via
            RTEF-1 downregulation
  JOURNAL   Aging Cell 13 (5), 926-934 (2014)
   PUBMED   25059272
  REMARK    GeneRIF: Dual-luciferase reporter gene analysis showed that RTEF-1
            is a direct target of mir-125a-5p, which regulates angiogenesis by
            repressing RTEF-1 expression and modulating eNOS and VEGF
            expression.
REFERENCE   9  (bases 1 to 1102)
  AUTHORS   Lorenzetti,D., Poirier,C., Zhao,M., Overbeek,P.A., Harrison,W. and
            Bishop,C.E.
  TITLE     A transgenic insertion on mouse chromosome 17 inactivates a novel
            immunoglobulin superfamily gene potentially involved in sperm-egg
            fusion
  JOURNAL   Mamm Genome 25 (3-4), 141-148 (2014)
   PUBMED   24275887
  REMARK    GeneRIF: The Spaca6 gene product may, together with Izumo1, mediate
            sperm fusion by binding an as yet unidentified egg membrane
            receptor.
REFERENCE   10 (bases 1 to 1102)
  AUTHORS   Kozomara,A. and Griffiths-Jones,S.
  TITLE     miRBase: integrating microRNA annotation and deep-sequencing data
  JOURNAL   Nucleic Acids Res 39 (Database issue), D152-D157 (2011)
   PUBMED   21037258
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC165361.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: single sample supports all introns SAMN00849381,
                              SAMN00849382 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-288               AC165361.3         72773-73060         c
            289-369             AC165361.3         71896-71976         c
            370-438             AC165361.3         67882-67950         c
            439-462             AC165361.3         67291-67314         c
            463-540             AC165361.3         66449-66526         c
            541-647             AC165361.3         66264-66370         c
            648-804             AC165361.3         65792-65948         c
            805-1015            AC165361.3         65509-65719         c
            1016-1102           AC165361.3         64964-65050         c
FEATURES             Location/Qualifiers
     source          1..1102
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 10.5 cM"
     gene            1..1102
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /note="sperm acrosome associated 6"
                     /db_xref="GeneID:75202"
                     /db_xref="MGI:MGI:1922452"
     exon            1..288
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /inference="alignment:Splign:2.1.0"
     CDS             30..1049
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /note="immunoglobulin domain-containing protein; sperm
                     acrosome membrane-associated protein 6"
                     /codon_start=1
                     /product="sperm acrosome membrane-associated protein 6
                     precursor"
                     /protein_id="NP_001156381.1"
                     /db_xref="CCDS:CCDS49967.1"
                     /db_xref="GeneID:75202"
                     /db_xref="MGI:MGI:1922452"
                     /translation="MTSQRSLSSPQTRRPSVMGLISLVGSIVLLFLLIFRASTWACLF
                     CFTTYEERLRVCQLFVGREETKINLCRNELEGAFEDLKDMKINYDERSYLHDEFTQMT
                     VSLQEKAARRREPFWLAFKDAAAKLKRTIEHLKKAPACIPPCGLQEVARLFHCSGCFS
                     KLCDLPLDCPVQDMLVNRGDQALFSCIVAFELPESEITYSWKFVGGVRTKDVTYFRDM
                     PGAHGYLARIRPVQPKHGGTFSCVILHDQRPLARLYFYLNVTGPPPPEDTELQVTFRE
                     VMNRTPAEPEMIQPWSPSLGELLTNPQALTLGNLFLLAATAALGSASVTLLVWLFFRW
                     YLSGN"
     exon            289..369
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /inference="alignment:Splign:2.1.0"
     exon            370..438
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /inference="alignment:Splign:2.1.0"
     exon            439..462
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /inference="alignment:Splign:2.1.0"
     exon            463..540
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /inference="alignment:Splign:2.1.0"
     exon            541..647
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /inference="alignment:Splign:2.1.0"
     exon            648..804
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /inference="alignment:Splign:2.1.0"
     exon            805..1015
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /inference="alignment:Splign:2.1.0"
     exon            1016..1102
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1078..1083
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /note="hexamer: AATAAA"
     polyA_site      1102
                     /gene="Spaca6"
                     /gene_synonym="4930546H06Rik; 9530079D04Rik;
                     B230206P06Rik; let-7e; mir-125a; mir-99b; Ncrna00085;
                     SPACA6A"
                     /note="major polyA site"
ORIGIN      
        1 ctctgatcac aggctccccc tcccccacaa tgacctcaca aaggtcacta tcttctcctc
       61 agacccggag accctctgtt atgggtctta tttccttagt agggagcatt gtcctgttgt
      121 tcctgctgat cttcagggca tccacgtggg cctgtctctt ctgcttcact acctatgagg
      181 aacgcctccg tgtctgccag ctgtttgtgg gcagagagga aaccaagata aacctgtgca
      241 gaaatgagct tgaaggggct tttgaagatc tcaaagacat gaaaatcaac tatgatgaga
      301 ggagctacct gcatgatgaa tttacacaga tgacagtttc tcttcaggag aaggctgcta
      361 gacgacggga gcccttttgg cttgccttca aagatgctgc tgcaaaattg aagaggacca
      421 ttgaacacct taaaaaagcc ccggcctgca tccctccttg tggtctccag gaggtggcta
      481 ggcttttcca ctgctcgggc tgcttttcta aactctgcga tctgcctctc gattgtccag
      541 ttcaggacat gctggtgaac cgaggggacc aggctttgtt ttcttgtatc gtggctttcg
      601 agttgccaga gtcggagatc acttattcct ggaaatttgt gggaggtgtc cgaactaagg
      661 acgttacgta cttccgtgat atgccggggg ctcacgggta cctggcacga atacggccag
      721 tgcaacccaa acacggtggg accttttcct gcgtgatcct gcacgaccag cgccccctgg
      781 cgcggctcta cttttatctg aacgtgacgg gccctcctcc gcctgaggac actgagctcc
      841 aggtcacgtt ccgggaagtg atgaatcgga cgccggcgga gccggaaatg atccagccct
      901 ggagccccag cctaggcgaa ctacttacca acccccaggc tctgacgctt ggcaacctat
      961 tcctgctcgc ggccaccgct gcacttgggt ctgccagtgt tactctgcta gtgtggctgt
     1021 tttttcgatg gtacttaagt ggcaactaac aaatcctgat cctacatctg tcctggaaat
     1081 aaagtatata ttttagttcg tt
//
