LOCUS       NM_027055                710 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus sperm acrosome associated 4 (Spaca4), mRNA.
ACCESSION   NM_027055
VERSION     NM_027055.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Fujihara,Y., Herberg,S., Blaha,A., Panser,K., Kobayashi,K.,
            Larasati,T., Novatchkova,M., Theussl,H.C., Olszanska,O., Ikawa,M.
            and Pauli,A.
  TITLE     The conserved fertility factor SPACA4/Bouncer has divergent modes
            of action in vertebrate fertilization
  JOURNAL   Proc Natl Acad Sci U S A 118 (39) (2021)
   PUBMED   34556579
  REMARK    GeneRIF: The conserved fertility factor SPACA4/Bouncer has
            divergent modes of action in vertebrate fertilization.
REFERENCE   2  (bases 1 to 710)
  AUTHORS   Loughner,C.L., Bruford,E.A., McAndrews,M.S., Delp,E.E.,
            Swamynathan,S. and Swamynathan,S.K.
  TITLE     Organization, evolution and functions of the human and mouse
            Ly6/uPAR family genes
  JOURNAL   Hum Genomics 10, 10 (2016)
   PUBMED   27098205
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 710)
  AUTHORS   Tang,T., Li,L., Tang,J., Li,Y., Lin,W.Y., Martin,F., Grant,D.,
            Solloway,M., Parker,L., Ye,W., Forrest,W., Ghilardi,N., Oravecz,T.,
            Platt,K.A., Rice,D.S., Hansen,G.M., Abuin,A., Eberhart,D.E.,
            Godowski,P., Holt,K.H., Peterson,A., Zambrowicz,B.P. and de
            Sauvage,F.J.
  TITLE     A mouse knockout library for secreted and transmembrane proteins
  JOURNAL   Nat Biotechnol 28 (7), 749-755 (2010)
   PUBMED   20562862
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC167242.4.
            
            On Apr 30, 2009 this sequence version replaced NM_027055.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript is intronless :: BC048608.1, BU961480.1 [ECO:0000345]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-710               AC167242.4         125767-126476       c
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.46 cM"
     gene            1..710
                     /gene="Spaca4"
                     /gene_synonym="1700008E09Rik; Samp14"
                     /note="sperm acrosome associated 4"
                     /db_xref="GeneID:69363"
                     /db_xref="MGI:MGI:1916613"
     exon            1..710
                     /gene="Spaca4"
                     /gene_synonym="1700008E09Rik; Samp14"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..624
                     /gene="Spaca4"
                     /gene_synonym="1700008E09Rik; Samp14"
                     /note="sperm acrosomal membrane 14; sperm acrosome
                     membrane-associated protein 4"
                     /codon_start=1
                     /product="sperm acrosome membrane-associated protein 4
                     precursor"
                     /protein_id="NP_081331.2"
                     /db_xref="CCDS:CCDS21262.1"
                     /db_xref="GeneID:69363"
                     /db_xref="MGI:MGI:1916613"
                     /translation="MVLGWPLLLVLVLCPGVTGIKDCVFCELTDSARCPGTHMRCGDD
                     EDCFTGHGVAQGVGPIINKGCVHSTSCGREEPISYMGLTYSLTTTCCSGHLCNKGTGL
                     STGATSLSLGLQLLLGLLLLLQYWL"
     regulatory      686..691
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spaca4"
                     /gene_synonym="1700008E09Rik; Samp14"
                     /note="hexamer: ATTAAA"
     polyA_site      710
                     /gene="Spaca4"
                     /gene_synonym="1700008E09Rik; Samp14"
                     /note="major polyA site"
ORIGIN      
        1 gaggtcactg agttccatgg cccaaaggct tgtgagcagg ggtgctggcc catctccaac
       61 ccgaggactg aagagcttct gaaaaggaca cgttgtatcg ggaacccgaa acactccaac
      121 tgctgacccc caaggccagg acgaggggca ggaggtgttc caggaggtca cactcggcca
      181 tccaggaggt caaggaagcc cccggcctgg cttgggtgct atccacgccg ccaggccacc
      241 atggtccttg gctggccact gcttctggtg ttggttcttt gcccaggtgt gacaggcatc
      301 aaggactgcg tcttctgtga gctgactgac tctgctcggt gccctggcac acacatgcgc
      361 tgtggggatg acgaagattg cttcacaggc cacggagtag cccagggtgt ggggcccatc
      421 atcaacaaag gctgcgtgca ctccaccagc tgtggccgcg aggaacccat cagctacatg
      481 ggcctcacat acagtctcac caccacctgc tgttctggcc acctttgcaa taagggcact
      541 ggcctttcca caggggctac cagcctgtca ctgggtctgc agctgctcct gggcctgttg
      601 ctgctgcttc aatactggct gtgacccaca agaactgcag ggcctagggc tgtgccccgg
      661 gcttccgcct tcatcccttt cccccattaa atggccagag gtgcagtcta
//
