LOCUS       NM_029367                941 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus sperm acrosome associated 3 (Spaca3), transcript
            variant 2, mRNA.
ACCESSION   NM_029367 XM_354632 XM_902822 XM_913064 XM_994623
VERSION     NM_029367.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Miyata,H., Castaneda,J.M., Fujihara,Y., Yu,Z., Archambeault,D.R.,
            Isotani,A., Kiyozumi,D., Kriseman,M.L., Mashiko,D., Matsumura,T.,
            Matzuk,R.M., Mori,M., Noda,T., Oji,A., Okabe,M.,
            Prunskaite-Hyyrylainen,R., Ramirez-Solis,R., Satouh,Y., Zhang,Q.,
            Ikawa,M. and Matzuk,M.M.
  TITLE     Genome engineering uncovers 54 evolutionarily conserved and
            testis-enriched genes that are not required for male fertility in
            mice
  JOURNAL   Proc Natl Acad Sci U S A 113 (28), 7704-7710 (2016)
   PUBMED   27357688
REFERENCE   2  (bases 1 to 941)
  AUTHORS   Zheng,H., Mandal,A., Shumilin,I.A., Chordia,M.D., Panneerdoss,S.,
            Herr,J.C. and Minor,W.
  TITLE     Sperm Lysozyme-Like Protein 1 (SLLP1), an intra-acrosomal
            oolemmal-binding sperm protein, reveals filamentous organization in
            protein crystal form
  JOURNAL   Andrology 3 (4), 756-771 (2015)
   PUBMED   26198801
  REMARK    GeneRIF: distinct from c-lysozyme by substituting two essential
            catalytic residues (E35T/D52N), and by forming helical filaments
            approximately 75 A in diameter with a 25 A central pore comprised
            of six monomers per helix turn repeating every 33 A
REFERENCE   3  (bases 1 to 941)
  AUTHORS   Wagner,A., Holland,O.J., Tong,M., Shelling,A.N. and Chamley,L.W.
  TITLE     The role of SPRASA in female fertility
  JOURNAL   Reprod Sci 22 (4), 452-461 (2015)
   PUBMED   25038051
  REMARK    GeneRIF: Female mice immunized with SPRASA had profound infertility
            following timed matings and those mice that did become pregnant had
            reduced fetal viability.
REFERENCE   4  (bases 1 to 941)
  AUTHORS   Sachdev,M., Mandal,A., Mulders,S., Digilio,L.C., Panneerdoss,S.,
            Suryavathi,V., Pires,E., Klotz,K.L., Hermens,L., Herrero,M.B.,
            Flickinger,C.J., van Duin,M. and Herr,J.C.
  TITLE     Oocyte specific oolemmal SAS1B involved in sperm binding through
            intra-acrosomal SLLP1 during fertilization
  JOURNAL   Dev Biol 363 (1), 40-51 (2012)
   PUBMED   22206759
  REMARK    GeneRIF: Data show that SAS1B/SAS1R (Sperm Acrosomal SLLP1
            Binding/Sperm Acrosomal SLLP1 receptor) bound to sperm acrosomal
            protein SlLP1 with high affinity.
REFERENCE   5  (bases 1 to 941)
  AUTHORS   Zhang,K., Gao,R., Zhang,H., Cai,X., Shen,C., Wu,C., Zhao,S. and
            Yu,L.
  TITLE     Molecular cloning and characterization of three novel lysozyme-like
            genes, predominantly expressed in the male reproductive system of
            humans, belonging to the c-type lysozyme/alpha-lactalbumin family
  JOURNAL   Biol Reprod 73 (5), 1064-1071 (2005)
   PUBMED   16014814
REFERENCE   6  (bases 1 to 941)
  AUTHORS   Herrero,M.B., Mandal,A., Digilio,L.C., Coonrod,S.A., Maier,B. and
            Herr,J.C.
  TITLE     Mouse SLLP1, a sperm lysozyme-like protein involved in sperm-egg
            binding and fertilization
  JOURNAL   Dev Biol 284 (1), 126-142 (2005)
   PUBMED   15982649
  REMARK    GeneRIF: This study demonstrates the retention of mouse sperm
            lysozyme-like protein (mSLLP1) in the equatorial segment of
            spermatozoa following the acrosome reaction and a role for mSLLP1
            in sperm-egg binding and fertilization.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC100503.1.
            
            On or before Jul 24, 2006 this sequence version replaced
            XM_354632.4, XM_902822.2, XM_913064.2, XM_994623.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR,
            compared to variant 1. Variants 1 and 2 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC100503.1, CA465759.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 48.4 cM"
     gene            1..941
                     /gene="Spaca3"
                     /gene_synonym="1700025M08Rik; ALLP17; Lyc3; mSLLP1; SLLP1"
                     /note="sperm acrosome associated 3"
                     /db_xref="GeneID:75622"
                     /db_xref="MGI:MGI:1922872"
     exon            1..133
                     /gene="Spaca3"
                     /gene_synonym="1700025M08Rik; ALLP17; Lyc3; mSLLP1; SLLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            134..399
                     /gene="Spaca3"
                     /gene_synonym="1700025M08Rik; ALLP17; Lyc3; mSLLP1; SLLP1"
                     /inference="alignment:Splign:2.1.0"
     CDS             213..704
                     /gene="Spaca3"
                     /gene_synonym="1700025M08Rik; ALLP17; Lyc3; mSLLP1; SLLP1"
                     /note="sperm c-type lysozyme-like protein; sperm acrosome
                     membrane-associated protein 3; lysozyme-like protein 3;
                     acrosomal sperm protein; sperm lysozyme-like protein 1
                     precusor"
                     /codon_start=1
                     /product="sperm acrosome membrane-associated protein 3
                     precursor"
                     /protein_id="NP_083643.1"
                     /db_xref="CCDS:CCDS25136.1"
                     /db_xref="GeneID:75622"
                     /db_xref="MGI:MGI:1922872"
                     /translation="MEARSRAPRRQLCPPGITWLALAYLLSCLLASSKAKVFSRCELA
                     KEMHDFGLDGYRGYNLADWVCLAYYTSGFNTNAVDHEADGSTNNGIFQISSRRWCRTL
                     ASNGPNLCRIYCTDLLNNDLKDSIVCAMKIVQEPLGLGYWEAWRHHCQGRDLSDWVDG
                     CDF"
     exon            400..558
                     /gene="Spaca3"
                     /gene_synonym="1700025M08Rik; ALLP17; Lyc3; mSLLP1; SLLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            559..637
                     /gene="Spaca3"
                     /gene_synonym="1700025M08Rik; ALLP17; Lyc3; mSLLP1; SLLP1"
                     /inference="alignment:Splign:2.1.0"
     exon            638..814
                     /gene="Spaca3"
                     /gene_synonym="1700025M08Rik; ALLP17; Lyc3; mSLLP1; SLLP1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      792..797
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spaca3"
                     /gene_synonym="1700025M08Rik; ALLP17; Lyc3; mSLLP1; SLLP1"
                     /note="hexamer: AATAAA"
     polyA_site      814
                     /gene="Spaca3"
                     /gene_synonym="1700025M08Rik; ALLP17; Lyc3; mSLLP1; SLLP1"
                     /note="major polyA site"
ORIGIN      
        1 gtgtctgatt tctcccgtgt tctctcaatg cctcgtgctg ggacactggc acgattacaa
       61 agggtgtcct gaaccgcatc aggacttgga gccacagcca ccgctgtcat cgtcccccac
      121 cggttcagct ctggggcagc tgtctgaacc cttgtgccgg gctggtgaac tgtctgccac
      181 cccacagttc agccctctac ctgtgccatc gcatggaagc taggagccgg gctcccagaa
      241 gacagctgtg cccgcctggg atcacttggc tggccctggc ctatctgctc agctgcctgc
      301 ttgcctccag caaggccaag gtcttcagtc gctgtgagct ggccaaagag atgcatgact
      361 tcggtctgga tggctaccgg ggttataacc tggctgactg ggtctgcctt gcttactaca
      421 caagtggctt caacacaaat gctgtggatc atgaagctga tggaagcacc aacaatggca
      481 tcttccagat cagcagccgg aggtggtgca gaaccctcgc ctcgaatggc cccaatcttt
      541 gcaggatata ctgcactgat ttgttgaaca atgatctcaa agattctatc gtctgtgcca
      601 tgaagatagt tcaagaaccc ctgggtctgg gctattggga agcctggagg caccactgcc
      661 agggcaggga cctcagtgac tgggtggatg gctgtgactt ctaggaccct ctctggatgg
      721 accaagccgc atacagctgg ctgggaagtg tggacttgtt cctgacccag gcttgggaag
      781 acaagccaag taataaaagt ctaatttgac agaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      841 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      901 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa a
//
