LOCUS       NM_026293               1073 bp    mRNA    linear   ROD 19-SEP-2024
DEFINITION  Mus musculus sperm acrosome associated 1 (Spaca1), transcript
            variant 1, mRNA.
ACCESSION   NM_026293
VERSION     NM_026293.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Jin,H.J., Fan,Y., Yang,X., Dong,Y., Zhang,X.Z., Geng,X.Y., Yan,Z.,
            Wu,L., Ma,M., Li,B., Lyu,Q., Pan,Y., Liu,M., Kuang,Y. and Chen,S.R.
  TITLE     Disruption in CYLC1 leads to acrosome detachment, sperm head
            deformity, and male in/subfertility in humans and mice
  JOURNAL   Elife 13, 95054 (2024)
   PUBMED   38573307
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   Dali,O., D'Cruz,S., Legoff,L., Diba Lahmidi,M., Heitz,C.,
            Merret,P.E., Kernanec,P.Y., Pakdel,F. and Smagulova,F.
  TITLE     Transgenerational epigenetic effects imposed by neonicotinoid
            thiacloprid exposure
  JOURNAL   Life Sci Alliance 7 (2), e202302237 (2023)
   PUBMED   37973188
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1073)
  AUTHORS   Yamatoya,K., Kousaka,M., Ito,C., Nakata,K., Hatano,M., Araki,Y. and
            Toshimori,K.
  TITLE     Cleavage of SPACA1 regulates assembly of sperm-egg membrane fusion
            machinery in mature spermatozoadagger
  JOURNAL   Biol Reprod 102 (3), 750-757 (2020)
   PUBMED   31836887
  REMARK    GeneRIF: Cleavage of SPACA1 regulates assembly of sperm-egg
            membrane fusion machinery in mature spermatozoadagger.
REFERENCE   4  (bases 1 to 1073)
  AUTHORS   Fujihara,Y., Oji,A., Larasati,T., Kojima-Kita,K. and Ikawa,M.
  TITLE     Human Globozoospermia-Related Gene Spata16 Is Required for Sperm
            Formation Revealed by CRISPR/Cas9-Mediated Mouse Models
  JOURNAL   Int J Mol Sci 18 (10), 2208 (2017)
   PUBMED   29065458
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1073)
  AUTHORS   Fujihara,Y., Satouh,Y., Inoue,N., Isotani,A., Ikawa,M. and Okabe,M.
  TITLE     SPACA1-deficient male mice are infertile with abnormally shaped
            sperm heads reminiscent of globozoospermia
  JOURNAL   Development 139 (19), 3583-3589 (2012)
   PUBMED   22949614
  REMARK    GeneRIF: SPACA1-deficient male mice are infertile with abnormally
            shaped sperm heads reminiscent of globozoospermia.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732568.8.
            
            On May 1, 2007 this sequence version replaced NM_026293.2.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK006187.1, BC147079.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-309               AL732568.8         32953-33261         c
            310-366             AL732568.8         27392-27448         c
            367-468             AL732568.8         25132-25233         c
            469-575             AL732568.8         24002-24108         c
            576-711             AL732568.8         22440-22575         c
            712-832             AL732568.8         12259-12379         c
            833-1073            AL732568.8         11508-11748         c
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 16.63 cM"
     gene            1..1073
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /note="sperm acrosome associated 1"
                     /db_xref="GeneID:67652"
                     /db_xref="MGI:MGI:1914902"
     exon            1..309
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..989
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; sperm acrosome membrane-associated protein 1"
                     /codon_start=1
                     /product="sperm acrosome membrane-associated protein 1
                     isoform 1 precursor"
                     /protein_id="NP_080569.1"
                     /db_xref="CCDS:CCDS18026.1"
                     /db_xref="GeneID:67652"
                     /db_xref="MGI:MGI:1914902"
                     /translation="MRARGAGCPAGLLAVGWLILVGLQASQASNVTSSGGGVQEPAVA
                     REGESESESESEEEAENEGEVPESETTAEADAEEEVQNRTIVKEVEFGMCTVTCGVGI
                     REVILTNGCPGGESKCVVRVEECRGPVDCGWGKPISENLDSARLSCVHISPENRFKYV
                     WKLLKPDQQPVILTNDSAVLEITREIRPLAFECDTLDNNEMVASVKFTVYTTNELQMR
                     RSSRPDTDAVLVFVLTIGVIICIFVIFVLIFIIINWAAVKSFWGSKTSATEIQSELSS
                     MRYKDSTSLDQSPTDIPVHEDDALSEWNE"
     exon            310..366
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            367..468
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            469..575
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            576..711
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            712..832
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            833..1073
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1051..1056
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /note="hexamer: AATAAA"
     polyA_site      1073
                     /gene="Spaca1"
                     /gene_synonym="1700124L11Rik; 4930540L03Rik"
                     /note="major polyA site"
ORIGIN      
        1 ctgttcgaag cagcctctcc tccagagccg gggctgcgac tgtgctttgg gcggccgtga
       61 ggccgagaag catgcgcgcc aggggcgcag gctgccccgc tgggctgctg gccgtcggat
      121 ggctgatcct ggtgggcctg caggcctcgc aggcatcgaa tgtcacctcc agcggcggag
      181 gcgtccagga acccgctgtg gcccgcgagg gcgagagcga gagcgagagc gagagtgagg
      241 aggaggctga gaacgaaggc gaagtgcccg agtccgaaac cacagccgag gccgatgccg
      301 aggaagaggt tcagaacaga acaatagtca aagaagtaga attcggaatg tgcacagtca
      361 cgtgtggtgt tggtatcaga gaagtaattt taaccaatgg atgccctgga ggtgaatcca
      421 agtgtgttgt tcgtgtggaa gaatgccgtg gacctgttga ttgtggctgg ggtaaaccaa
      481 tttctgaaaa tcttgatagt gctagactat catgtgttca tatatctcct gaaaatcgtt
      541 tcaaatatgt atggaagctt ctaaagccag accaacaacc tgttatactg acaaatgact
      601 cagcagtctt ggaaataacg agagaaattc gtcctctggc ttttgaatgt gacaccttgg
      661 ataataatga aatggtggca tctgttaaat tcaccgtcta tacaacaaat gaactgcaga
      721 tgagaagatc cagcagacca gacactgatg cagtcttagt tttcgtgctg accataggag
      781 tcattatctg catatttgtg atcttcgtat tgattttcat catcataaat tgggcggcag
      841 tgaaatcctt ctgggggtca aaaacttcag caactgagat acagtccgag ctgagctcta
      901 tgaggtataa agattcgacg tctctcgacc agtcaccaac agatatacct gtacatgaag
      961 atgacgcttt aagtgaatgg aatgaatgat gtatggatga catatgagaa accagagacc
     1021 attagagcat atcagattca ttattatcaa aataaaatat atattggaaa ttt
//
