LOCUS       NM_024449               2066 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sclerostin (Sost), mRNA.
ACCESSION   NM_024449
VERSION     NM_024449.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Pina,J.O., Raju,R., Roth,D.M., Winchester,E.W., Padilla,C.,
            Iben,J., Faucz,F.R., Cotney,J.L. and D'Souza,R.N.
  TITLE     Spatial Multi-omics Reveals the Role of the Wnt Modulator, Dkk2, in
            Palatogenesis'
  JOURNAL   J Dent Res 103 (13), 1412-1420 (2024)
   PUBMED   38910391
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Baroi,S., Czernik,P.J., Khan,M.P., Letson,J., Crowe,E.,
            Chougule,A., Griffin,P.R., Rosen,C.J. and Lecka-Czernik,B.
  TITLE     PPARG in osteocytes controls cell bioenergetics and systemic energy
            metabolism independently of sclerostin levels in circulation
  JOURNAL   Mol Metab 88, 102000 (2024)
   PUBMED   39074536
  REMARK    GeneRIF: PPARG in osteocytes controls cell bioenergetics and
            systemic energy metabolism independently of sclerostin levels in
            circulation.
REFERENCE   3  (bases 1 to 2066)
  AUTHORS   Morfin,C., Sebastian,A., Wilson,S.P., Amiri,B., Murugesh,D.K.,
            Hum,N.R., Christiansen,B.A. and Loots,G.G.
  TITLE     Mef2c regulates bone mass through Sost-dependent and -independent
            mechanisms
  JOURNAL   Bone 179, 116976 (2024)
   PUBMED   38042445
  REMARK    GeneRIF: Mef2c regulates bone mass through Sost-dependent and
            -independent mechanisms.
REFERENCE   4  (bases 1 to 2066)
  AUTHORS   Raju,R., Pina,J.O., Roth,D.M., Chattaraj,P., Kidwai,F.K.,
            Faucz,F.R., Iben,J., Fridell,G., Dale,R.K. and D'Souza,R.N.
  TITLE     Profiles of Wnt pathway gene expression during tooth morphogenesis
  JOURNAL   Front Physiol 14, 1316635 (2024)
   PUBMED   38274045
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2066)
  AUTHORS   Pina,J.O., Raju,R., Roth,D.M., Winchester,E.W., Chattaraj,P.,
            Kidwai,F., Faucz,F.R., Iben,J., Mitra,A., Campbell,K., Fridell,G.,
            Esnault,C., Cotney,J.L., Dale,R.K. and D'Souza,R.N.
  TITLE     Multimodal spatiotemporal transcriptomic resolution of embryonic
            palate osteogenesis
  JOURNAL   Nat Commun 14 (1), 5687 (2023)
   PUBMED   37709732
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2066)
  AUTHORS   Winkler,D.G., Yu,C., Geoghegan,J.C., Ojala,E.W., Skonier,J.E.,
            Shpektor,D., Sutherland,M.K. and Latham,J.A.
  TITLE     Noggin and sclerostin bone morphogenetic protein antagonists form a
            mutually inhibitory complex
  JOURNAL   J Biol Chem 279 (35), 36293-36298 (2004)
   PUBMED   15199066
  REMARK    GeneRIF: noggin and sclerostin form a mutually inhibitory complex
            that fine-tunes of BMP activity in bone homeostasis
REFERENCE   7  (bases 1 to 2066)
  AUTHORS   Winkler,D.G., Sutherland,M.K., Geoghegan,J.C., Yu,C., Hayes,T.,
            Skonier,J.E., Shpektor,D., Jonas,M., Kovacevich,B.R.,
            Staehling-Hampton,K., Appleby,M., Brunkow,M.E. and Latham,J.A.
  TITLE     Osteocyte control of bone formation via sclerostin, a novel BMP
            antagonist
  JOURNAL   EMBO J 22 (23), 6267-6276 (2003)
   PUBMED   14633986
REFERENCE   8  (bases 1 to 2066)
  AUTHORS   Kusu,N., Laurikkala,J., Imanishi,M., Usui,H., Konishi,M.,
            Miyake,A., Thesleff,I. and Itoh,N.
  TITLE     Sclerostin is a novel secreted osteoclast-derived bone
            morphogenetic protein antagonist with unique ligand specificity
  JOURNAL   J Biol Chem 278 (26), 24113-24117 (2003)
   PUBMED   12702725
  REMARK    GeneRIF: effects of sclerostin on the activity of BMP2, BMP4, BMP6,
            and BMP7 and role in bone remodeling
REFERENCE   9  (bases 1 to 2066)
  AUTHORS   Heller,R.S., Dichmann,D.S., Jensen,J., Miller,C., Wong,G.,
            Madsen,O.D. and Serup,P.
  TITLE     Expression patterns of Wnts, Frizzleds, sFRPs, and misexpression in
            transgenic mice suggesting a role for Wnts in pancreas and foregut
            pattern formation
  JOURNAL   Dev Dyn 225 (3), 260-270 (2002)
   PUBMED   12412008
REFERENCE   10 (bases 1 to 2066)
  AUTHORS   Brunkow,M.E., Gardner,J.C., Van Ness,J., Paeper,B.W.,
            Kovacevich,B.R., Proll,S., Skonier,J.E., Zhao,L., Sabo,P.J., Fu,Y.,
            Alisch,R.S., Gillett,L., Colbert,T., Tacconi,P., Galas,D.,
            Hamersma,H., Beighton,P. and Mulligan,J.
  TITLE     Bone dysplasia sclerosteosis results from loss of the SOST gene
            product, a novel cystine knot-containing protein
  JOURNAL   Am J Hum Genet 68 (3), 577-589 (2001)
   PUBMED   11179006
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK017295.1, AK041382.1 and BY593587.1.
            
            On Dec 18, 2013 this sequence version replaced NM_024449.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK041382.1, AK017295.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1804              AK017295.1         2-1805
            1805-1892           AK041382.1         1808-1895
            1893-2062           AK041382.1         1898-2067
            2063-2066           BY593587.1         199-202
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 65.48 cM"
     gene            1..2066
                     /gene="Sost"
                     /gene_synonym="5430411E23Rik"
                     /note="sclerostin"
                     /db_xref="GeneID:74499"
                     /db_xref="MGI:MGI:1921749"
     exon            1..256
                     /gene="Sost"
                     /gene_synonym="5430411E23Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..678
                     /gene="Sost"
                     /gene_synonym="5430411E23Rik"
                     /codon_start=1
                     /product="sclerostin precursor"
                     /protein_id="NP_077769.4"
                     /db_xref="CCDS:CCDS25481.1"
                     /db_xref="GeneID:74499"
                     /db_xref="MGI:MGI:1921749"
                     /translation="MQPSLAPCLICLLVHAAFCAVEGQGWQAFRNDATEVIPGLGEYP
                     EPPPENNQTMNRAENGGRPPHHPYDAKDVSEYSCRELHYTRFLTDGPCRSAKPVTELV
                     CSGQCGPARLLPNAIGRVKWWRPNGPDFRCIPDRYRAQRVQLLCPGGAAPRSRKVRLV
                     ASCKCKRLTRFHNQSELKDFGPETARPQKGRKPRPGARGAKANQAELENAY"
     exon            257..2066
                     /gene="Sost"
                     /gene_synonym="5430411E23Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2043..2048
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sost"
                     /gene_synonym="5430411E23Rik"
                     /note="hexamer: AATAAA"
     polyA_site      2063
                     /gene="Sost"
                     /gene_synonym="5430411E23Rik"
                     /note="major polyA site"
ORIGIN      
        1 actggagcct gtgctaccga gtgccctcct ccacctggca gcatgcagcc ctcactagcc
       61 ccgtgcctca tctgcctact tgtgcacgct gccttctgtg ctgtggaggg ccaggggtgg
      121 caagccttca ggaatgatgc cacagaggtc atcccagggc ttggagagta ccccgagcct
      181 cctcctgaga acaaccagac catgaaccgg gcggagaatg gaggcagacc tccccaccat
      241 ccctatgacg ccaaagatgt gtccgagtac agctgccgcg agctgcacta cacccgcttc
      301 ctgacagacg gcccatgccg cagcgccaag ccggtcaccg agttggtgtg ctccggccag
      361 tgcggccccg cgcggctgct gcccaacgcc atcgggcgcg tgaagtggtg gcgcccgaac
      421 ggaccggatt tccgctgcat cccggatcgc taccgcgcgc agcgggtgca gctgctgtgc
      481 cccgggggcg cggcgccgcg ctcgcgcaag gtgcgtctgg tggcctcgtg caagtgcaag
      541 cgcctcaccc gcttccacaa ccagtcggag ctcaaggact tcgggccgga gaccgcgcgg
      601 ccgcagaagg gtcgcaagcc gcggcccggc gcccggggag ccaaagccaa ccaggcggag
      661 ctggagaacg cctactagag cgagcccgcg cctatgcagc ccccgcgcga tccgattcgt
      721 tttcagtgta aagcctgcag cccaggccag gggtgccaaa ctttccagac cgtgtggagt
      781 tcccagccca gtagagaccg caggtccttc tgcccgctgc gggggatggg gagggggtgg
      841 ggttcccgcg ggccaggaga ggaagcttga gtcccagact ctgcctagcc ccgggtggga
      901 tgggggtctt tctaccctcg ccggacctat acaggacaag gcagtgtttc caccttaaag
      961 ggaagggagt gtggaacgaa agacctggga ctggttatgg acgtacagta agatctactc
     1021 cttccaccca aatgtaaagc ctgcgtgggc tagatagggt ttctgaccct gacctggcca
     1081 ctgagtgtga tgttgggcta cgtggttctc ttttggtacg gtcttctttg taaaataggg
     1141 accggaactc tgctgagatt ccaaggattg gggtaccccg tgtagactgg tgagagagag
     1201 gagaacaggg gaggggttag gggagagatt gtggtgggca accgcctaga agaagctgtt
     1261 tgttggctcc cagcctcgcc gcctcagagg tttggcttcc cccactcctt cctctcaaat
     1321 ctgccttcaa atccatatct gggataggga aggccagggt ccgagagatg gtggaagggc
     1381 cagaaatcac actcctggcc ccccgaagag cagtgtcccg cccccaactg ccttgtcata
     1441 ttgtaaaggg attttctaca caacagttta aggtcgttgg aggaaactgg gcttgccagt
     1501 cacctcccat ccttgtccct tgccaggaca ccacctcctg cctgccaccc acggacacat
     1561 ttctgtctag aaacagagcg tcgtcgtgct gtcctctgag acagcatatc ttacattaaa
     1621 aagaataata cggggggggg ggcggagggc gcaagtgtta tacatatgct gagaagctgt
     1681 caggcgccac agcaccaccc acaatctttt tgtaaatcat ttccagacac ctcttacttt
     1741 ctgtgtagat tttaattgtt aaaaggggag gagagagagc gtttgtaaca gaagcacatg
     1801 gagggggggg taggggggtt ggggctggtg agtttggcga actttccatg tgagactcat
     1861 ccacaaagac tgaaagccgc gttttttttt ttaagagttc agtgacatat ttattttctc
     1921 atttaagtta tttatgccaa catttttttc ttgtagagaa aggcagtgtt aatatcgctt
     1981 tgtgaagcac aagtgtgtgt ggttttttgt tttttgtttt ttccccgacc agaggcattg
     2041 ttaataaaga caatgaatct cgagca
//
