LOCUS       NM_009231               8919 bp    mRNA    linear   ROD 04-JUN-2024
DEFINITION  Mus musculus SOS Ras/Rac guanine nucleotide exchange factor 1
            (Sos1), mRNA.
ACCESSION   NM_009231
VERSION     NM_009231.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 8919)
  AUTHORS   Baltanas,F.C., Garcia-Navas,R., Rodriguez-Ramos,P., Calzada,N.,
            Cuesta,C., Borrajo,J., Fuentes-Mateos,R., Olarte-San Juan,A.,
            Vidana,N., Castellano,E. and Santos,E.
  TITLE     Critical requirement of SOS1 for tumor development and
            microenvironment modulation in KRASG12D-driven lung adenocarcinoma
  JOURNAL   Nat Commun 14 (1), 5856 (2023)
   PUBMED   37730692
  REMARK    GeneRIF: Critical requirement of SOS1 for tumor development and
            microenvironment modulation in KRAS[G12D]-driven lung
            adenocarcinoma.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8919)
  AUTHORS   Ni,Q.Z., Zhu,B., Ji,Y., Zheng,Q.W., Liang,X., Ma,N., Jiang,H.,
            Zhang,F.K., Shang,Y.R., Wang,Y.K., Xu,S., Zhang,E.B., Yuan,Y.M.,
            Chen,T.W., Yin,F.F., Cao,H.J., Huang,J.Y., Xia,J., Ding,X.F.,
            Qiu,X.S., Ding,K., Song,C., Zhou,W.T., Wu,M., Wang,K., Lui,R.,
            Lin,Q., Chen,W., Li,Z.G., Cheng,S.Q., Wang,X.F., Xie,D. and Li,J.J.
  TITLE     PPDPF Promotes the Development of Mutant KRAS-Driven Pancreatic
            Ductal Adenocarcinoma by Regulating the GEF Activity of SOS1
  JOURNAL   Adv Sci (Weinh) 10 (2), e2202448 (2023)
   PUBMED   36453576
  REMARK    GeneRIF: PPDPF Promotes the Development of Mutant KRAS-Driven
            Pancreatic Ductal Adenocarcinoma by Regulating the GEF Activity of
            SOS1.
REFERENCE   3  (bases 1 to 8919)
  AUTHORS   Padmanabhan,R.A., Zyju,D.P., Subramaniam,A.G., Nautiyal,J. and
            Laloraya,M.
  TITLE     Son of sevenless 1 (SOS1), the RasGEF, interacts with ERalpha and
            STAT3 during embryo implantation
  JOURNAL   J Mol Endocrinol 70 (1), e220089 (2022)
   PUBMED   36103132
  REMARK    GeneRIF: Son of sevenless 1 (SOS1), the RasGEF, interacts with
            ERalpha and STAT3 during embryo implantation.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8919)
  AUTHORS   Gomez,C., Garcia-Navas,R., Baltanas,F.C., Fuentes-Mateos,R.,
            Fernandez-Medarde,A., Calzada,N. and Santos,E.
  TITLE     Critical Requirement of SOS1 for Development of BCR/ABL-Driven
            Chronic Myelogenous Leukemia
  JOURNAL   Cancers (Basel) 14 (16), 3893 (2022)
   PUBMED   36010887
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 8919)
  AUTHORS   Geng,X., Chen,L., Srinivasan,R.S., Kylat,R.J., Witte,M.H. and
            Erickson,R.J.
  TITLE     Lack of embryonic homozygous or adult heterozygous lymphatic
            phenotypes for a Sos1 mutation and lack of lymphatic embryonic
            phenotypes for a homozygous Cx47 mutation in mice
  JOURNAL   Lymphology 55 (3), 129-134 (2022)
   PUBMED   36446400
  REMARK    GeneRIF: Lack of embryonic homozygous or adult heterozygous
            lymphatic phenotypes for a Sos1 mutation and lack of lymphatic
            embryonic phenotypes for a homozygous Cx47 mutation in mice.
REFERENCE   6  (bases 1 to 8919)
  AUTHORS   Oliver,G., Wehr,R., Jenkins,N.A., Copeland,N.G., Cheyette,B.N.,
            Hartenstein,V., Zipursky,S.L. and Gruss,P.
  TITLE     Homeobox genes and connective tissue patterning
  JOURNAL   Development 121 (3), 693-705 (1995)
   PUBMED   7720577
REFERENCE   7  (bases 1 to 8919)
  AUTHORS   Sakaue,M., Bowtell,D. and Kasuga,M.
  TITLE     A dominant-negative mutant of mSOS1 inhibits insulin-induced Ras
            activation and reveals Ras-dependent and -independent insulin
            signaling pathways
  JOURNAL   Mol Cell Biol 15 (1), 379-388 (1995)
   PUBMED   7799946
REFERENCE   8  (bases 1 to 8919)
  AUTHORS   Mathew,P., Morris,S.W., Kane,J.R., Shurtleff,S.A., Pasquini,M.,
            Jenkins,N.A., Gilbert,D.J. and Copeland,N.G.
  TITLE     Localization of the murine homolog of the anaplastic lymphoma
            kinase (AlK) gene on mouse chromosome 17
  JOURNAL   Cytogenet Cell Genet 70 (1-2), 143-144 (1995)
   PUBMED   7736780
REFERENCE   9  (bases 1 to 8919)
  AUTHORS   den Hertog,J., Tracy,S. and Hunter,T.
  TITLE     Phosphorylation of receptor protein-tyrosine phosphatase alpha on
            Tyr789, a binding site for the SH3-SH2-SH3 adaptor protein GRB-2 in
            vivo
  JOURNAL   EMBO J 13 (13), 3020-3032 (1994)
   PUBMED   7518772
REFERENCE   10 (bases 1 to 8919)
  AUTHORS   Bowtell,D., Fu,P., Simon,M. and Senior,P.
  TITLE     Identification of murine homologues of the Drosophila son of
            sevenless gene: potential activators of ras
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6511-6515 (1992)
   PUBMED   1631150
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK140310.1, CB521016.1 and AC161447.6.
            
            On Nov 3, 2006 this sequence version replaced NM_009231.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14995047.1077988.1,
                                           SRR7652917.1248123.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3879              AK140310.1         1-3879
            3880-4522           CB521016.1         44-686
            4523-8919           AC161447.6         114568-118964
FEATURES             Location/Qualifiers
     source          1..8919
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 50.67 cM"
     gene            1..8919
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /note="SOS Ras/Rac guanine nucleotide exchange factor 1"
                     /db_xref="GeneID:20662"
                     /db_xref="MGI:MGI:98354"
     exon            1..617
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     CDS             531..4490
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /note="guanine nucleotide releasing factor;
                     son-of-sevenless 1; ras guanine exchange factor"
                     /codon_start=1
                     /product="son of sevenless homolog 1"
                     /protein_id="NP_033257.2"
                     /db_xref="CCDS:CCDS37704.1"
                     /db_xref="GeneID:20662"
                     /db_xref="MGI:MGI:98354"
                     /translation="MQAQQLPYEFFSEENAPKWRGLLVPALKKVQGQVHPTLESNDDA
                     LQYVEELILQLLNMLCQAQPRSASDVEERVQKSFPHPIDKWAIADAQSAIEKRKRRNP
                     LSLPAERIHHLLREVLGYKIDHQVSVYIVAVLEYISADILKLVGNYVRNIRHYEITKQ
                     DIKVAMCADKVLMDMFHQDVEDINILSLTDEEPSTSGEQTYYDLVKAFMAEIRQYIRE
                     LNLIIKVFREPFVSNSKLFSSNDVENIFSRIVDIHELSVKLLGHIEDTVEMTDEGSPH
                     PLVGSCFEDLAEELAFDPYESYARDILRPGFHGHFLSQLSKPGAALYLQSIGEGFKEA
                     VQYVLPRLLLAPVYHCLHYFELLKQLEEKSEDQEDKECMKQAITALLNVQSGMEKICS
                     KSLAKRRLSESACRFYSQQMKGKQLAIKKMNEIQKNIDGWEGKDIGQCCNEFIMEGTL
                     TRVGAKHERHIFLFDGLMICCKSNHGQPRLPGASSAEYRLKEKFFMRKVQINDKDDTS
                     EYKHAFEIILKDGNSVIFSAKSAEEKNNWMAALISLQYRSTLERMLDVTVLQEEKEEQ
                     MRLPSAEVYRFAEPDSEENILFEENVQPKAGIPIIKAGTVLKLIERLTYHMYADPNFV
                     RTFLTTYRSFCRPQELLSLLIERFEIPEPEPTEADRIAIENGDQPLSAELKRFRKEYI
                     QPVQLRVLNVCRHWVEHHFYDFERDADLLQRMEEFIGTVRGKAMKKWVESITKIIQRK
                     KIARDNGPGHNITFQSSPPTVEWHISRPGHIETFDLLTLHPIEIARQLTLLESDLYRA
                     VQPSELVGSVWTKEDKEINSPNLLKMIRHTTNLTLWFEKCIVETENLEERVAVVSRII
                     EILQVFQELNNFNGVLEVVSAMNSSPVYRLDHTFEQIPSRQKKILEEAHELSEDHYKK
                     YLAKLRSINPPCVPFFGIYLTNILKTEEGNPEVLRRHGKELINFSKRRRVAEITGEIQ
                     QYQNQPYCLRVEPDIKRFFENLNPMGNSMEKEFTDYLFNKSLEIEPRHPKPLPRFPKK
                     YSYPLKSPGVRPSNPRPGTMRHPTPLQQEPRKISYSRIPESETESTASAPNSPRTPLT
                     PPPASGTSSNTDVCSVFDSDHSASPFHSRSASVSSISLSKGTDEVPVPPPVPPRRRPE
                     SAPAESSPSKIMSKHLDSPPAIPPRQPTSKAYSPRYSISDRTSISDPPESPPLLPPRE
                     PVRTPDVFSSSPLHLQPPPLGKKSDHGNAFFPNSPSPFTPPPPQTPSPHGTRRHLPSP
                     PLTQEMDLHSIAGPPVPPRQSTSQLIPKLPPKTYKREHTHPSMHRDGPPLLENAHSS"
     exon            618..743
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            744..875
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            876..1040
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1250
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1251..1394
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1395..1505
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1506..1604
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1605..1732
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            1733..2388
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            2389..2470
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            2471..2593
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            2594..2697
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            2698..2920
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            2921..3040
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            3041..3203
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            3204..3321
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            3322..3494
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            3495..3611
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            3612..3879
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            3880..3998
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     exon            3999..8919
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5517..5522
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /note="hexamer: AATAAA"
     polyA_site      5547
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /note="major polyA site"
     regulatory      5551..5556
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /note="hexamer: ATTAAA"
     polyA_site      5573
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /note="major polyA site"
     regulatory      8628..8633
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /note="hexamer: AATAAA"
     polyA_site      8655
                     /gene="Sos1"
                     /gene_synonym="4430401P03Rik; 9630010N06"
                     /note="major polyA site"
ORIGIN      
        1 agtttgctag taggaagctc cggcgctaca ccggcggcgg cagcggcagc ggtagcagca
       61 gcggcggcga cggccaagcg gccggattcc ccggcagcga cctcgggccc tccccgttcc
      121 ccaccgaccc ctgctcccgc ttcgtcccgt tctgggcctc tcgcttgagt tccccgctgg
      181 cgagcgggcg ccaccggccg gagcgtccat ccacgcgccg tctctcggcg ggccaggccg
      241 tgccggggag cgcagccccg gcggagggcg gcccggggcg gagcgggggg cctgccccgg
      301 ggcggtggtg cgggatgccg ccgccgccgc cgccgggttg cgttggctct cggcgagcgg
      361 cgcgcgtgtc gggtgggtgt ccggccgcgg gcctcgcccc ccagactcct cgccagcgct
      421 agccgggccg cgcggcgccc cgagggggcc gggccgtgca cgggctgtgg cccttctccg
      481 cgccgcgagc cgccgcccct ccgcccgccc cgaggcgccc cgcgggcacc atgcaggcgc
      541 agcagctgcc ttacgagttt ttcagcgagg agaacgcgcc caagtggcgg gggctgctgg
      601 tgcctgcgct gaaaaaggtt caggggcaag ttcaccctac tcttgagtct aatgatgatg
      661 ctcttcagta tgttgaagaa ttaattttgc aattactaaa tatgctatgc caagctcagc
      721 cccggagtgc ttcagatgtg gaggaacgtg ttcaaaagag ttttcctcat ccaattgata
      781 agtgggcaat agctgatgcc caatcagcca ttgaaaagag gaagagacga aatcctttat
      841 cgctgccagc agaaagaatt catcatttat taagggaggt cctcggttat aaaattgacc
      901 accaggtttc tgtttacata gtagcagtat tagaatacat ttctgcagat attttaaagc
      961 tcgtggggaa ttatgtaaga aatatacggc attatgaaat tacaaaacaa gacattaaag
     1021 tggcaatgtg tgctgataag gtattgatgg atatgtttca tcaagatgta gaagatataa
     1081 atatcttatc tttaactgat gaagagcctt ccacctcagg agaacaaact tattatgatt
     1141 tggtaaaagc attcatggca gaaattcgac agtatataag agaattaaat ctaattataa
     1201 aagtttttcg agagcccttt gtctctaatt ccaaattgtt ttcatctaat gatgtagaaa
     1261 acatattcag tcgtatagta gatatacatg aacttagtgt aaagttactg ggccatatag
     1321 aagatactgt agaaatgaca gatgaaggca gtccccaccc attagtagga agctgttttg
     1381 aagacttagc agaagaactg gcatttgacc cgtatgagtc atatgctcgg gatattttac
     1441 gacccggatt ccatggccat tttcttagtc agttatcaaa gcctggggca gcactttatt
     1501 tgcagtccat aggcgaaggc ttcaaagaag ctgtccagta cgtcctgccc cggctgctgc
     1561 ttgcccctgt gtaccactgt ctgcattact ttgaacttct gaagcagtta gaagaaaaga
     1621 gtgaagatca agaagacaag gagtgtatga agcaagcaat aacagccctg cttaatgtcc
     1681 aaagtggcat ggaaaaaatt tgctccaaaa gtcttgcaaa acgaagacta agtgagtctg
     1741 catgtcggtt ttacagccag cagatgaagg ggaaacagct agccatcaag aagatgaacg
     1801 agatccagaa gaacattgat ggctgggagg ggaaggacat tggacagtgt tgcaatgagt
     1861 tcataatgga aggaactctt acacgtgtag gagccaaaca cgagagacac atatttctct
     1921 tcgatggctt aatgatttgc tgtaaatcaa accatgggca gccaagactc cctggtgcta
     1981 gcagtgcaga ataccggctt aaagaaaagt tttttatgcg aaaggtacag attaatgata
     2041 aagatgacac cagtgagtac aagcatgctt ttgaaatcat tctgaaagat ggcaatagtg
     2101 ttatattttc tgccaagtca gctgaagaga aaaacaactg gatggcagca ctgatctctt
     2161 tgcagtaccg cagcaccctg gagaggatgc tggacgtaac ggtgctgcag gaggagaagg
     2221 aggagcagat gaggctgccc agtgctgaag tgtacaggtt tgcagaacct gactccgagg
     2281 agaatattct attcgaagag aatgtgcagc ccaaagctgg gatccccatt atcaaggcag
     2341 ggacagtgct taagctcatt gagaggctta cctaccacat gtacgcagat ccaaattttg
     2401 ttcggacgtt tcttacaaca tacaggtcct tttgcagacc tcaagaacta ctgagtcttc
     2461 tgatagaaag atttgaaatt ccagagcctg agccaacaga agctgatcgc atagctatag
     2521 agaatggaga tcagcccctg agtgcagagc tgaagaggtt tagaaaggaa tatattcagc
     2581 ctgtgcagtt gagggtgtta aatgtgtgtc ggcactgggt ggagcaccat ttctatgact
     2641 ttgaaagaga tgcagacctt ttacagagaa tggaggaatt tattggaaca gtaagaggta
     2701 aagcaatgaa aaaatgggtc gaatccatca ctaagataat ccaaaggaaa aaaattgcaa
     2761 gagacaatgg cccaggtcat aacattacat ttcagagctc acctcccaca gttgagtggc
     2821 acataagcag acctgggcac atagagactt ttgacttgct caccttacac ccaatagaaa
     2881 ttgctcggca actcacttta cttgaatcag atctataccg ggctgtgcag ccatcagaat
     2941 tagttggaag tgtgtggaca aaagaagata aagaaattaa ttctcccaac cttctgaaga
     3001 tgattcggca caccactaac ctcactttgt ggtttgagaa atgtattgta gaaacagaaa
     3061 acttagaaga aagagtagct gtagtaagtc ggataattga gattctacaa gtctttcaag
     3121 agctgaacaa cttcaatggt gtcctggaag ttgtcagtgc tatgaactcg tcacctgttt
     3181 acagactaga ccacacattt gagcaaatac caagcagaca aaagaaaatt ttagaagaag
     3241 ctcatgaatt gagtgaagat cactataaga aatatttggc aaaactcagg tctattaatc
     3301 caccgtgtgt gcctttcttt ggaatttatc tcacaaatat cctgaagaca gaagagggca
     3361 accctgaggt cctgaggaga cacgggaaag agcttattaa cttcagcaag aggaggagag
     3421 tggccgagat cacaggcgag atccagcagt accagaacca gccctactgc ttacgggtgg
     3481 agccggacat caagaggttc tttgaaaact tgaatccaat gggaaacagc atggagaaag
     3541 aatttacaga ctatctgttc aacaaatccc tagaaataga accccggcac cctaagcctc
     3601 ttccgagatt cccaaaaaaa tacagctatc ccctaaaatc tcctggtgtt cgtccatcaa
     3661 atccaagacc aggaaccatg agacatccca cacctctgca gcaggagcca agaaaaatta
     3721 gctacagtcg gattcctgaa agtgagacgg aaagcacagc atctgcacca aactcccctc
     3781 ggaccccact gacgccgccc cctgcatctg gcacctccag caacacagat gtttgcagcg
     3841 tgttcgattc tgaccactcg gcaagccctt ttcattcaag atctgcttca gtctcatcta
     3901 taagtttatc caagggcact gatgaagtgc ctgtcccccc tcctgtaccc cctcgaagac
     3961 gtccagagtc tgccccagct gaatcctccc catccaagat tatgtctaag cacttggaca
     4021 gccccccagc tattcctcct aggcaaccca catccaaagc ctattcacca cgctattcaa
     4081 tatcagatcg gacctctata tcagatcctc ctgaaagccc tcccttgtta ccaccacggg
     4141 aacctgtgag gacacctgat gttttctcaa gctcaccatt acatctccaa cctcctcctt
     4201 tgggcaaaaa gagtgatcat ggcaacgcct tcttcccaaa cagcccatcc ccttttacac
     4261 cgccaccccc ccaaaccccc tctcctcatg gcacgagaag gcatctgcca tcaccaccac
     4321 tgacacagga gatggacctc cattccattg ctgggcctcc tgttcctcca cgacaaagca
     4381 cttctcaact tatccccaaa ctccctccaa aaacttacaa aagggagcac acacacccat
     4441 ccatgcatag agatggacca ccactgctgg agaatgccca ttcttcctga gttcctctaa
     4501 gctgggatag tttcctagcc cccagatcca ttgctggcaa tggatgcact gaacatgcca
     4561 gcactgggga gttcaaatga gaactccaaa cactaacgac tctacttcac gatgtagtat
     4621 aagacaatga gttttaacct acatggaatt atggaataaa atggtattcc agcttagaat
     4681 gtggaaactg attgcacctg gaaatcacgt gaagggactt ttctggccat tgggcaggag
     4741 tcctcatatt gtgaagtgat ctttatcatt aaagggatgg aaaacagtct aatgtccaac
     4801 aagcccatat gttgacagtt tttgtaattc aaaatattat gcacttttaa aaaatcttaa
     4861 acagggatct cctcctttgt tttcctttgc tttactcttc tactttagaa tattttcgta
     4921 aaagttattc agaggactgt gagaaaaggc tgtggtacct gaccttgttg aaatcaaggc
     4981 ccagcactgt actacagtcc tgtttacaga ttattacagt gatctgaatg ggtaccgagg
     5041 cttcaccaaa gaggtacttt ttgttattgt tattgtttta agaataatta tgccaatttt
     5101 aagaacatcc cctacccacc cccactcaca aacaaaatgt ggtggtgttg cctttaaaca
     5161 aaaaatgtca atgtcattaa catgatggaa gaagaacatt ttaaaacgta actgtcaagt
     5221 atacgattta cgcttaggag actgttcatc tatgctagac tgtcatcccc cagccccttt
     5281 gtctcacaga agtttactgg taatgtatgt cctggctcgt ggctgtccct ctaaccgtac
     5341 acagaaacac aggcacccaa tgtgaacgag agcactctct gggcctccct gacacctttc
     5401 atgttttact aatagttgag tagttagcat acctagaatt accttgcatt gcctaaatca
     5461 tgtgtatata gtgaatgtta tataatatac cttgcaggtc tgttattatt taattgaata
     5521 aagataaact actttgtttg gctggcatat attaaattat tatatggaga aaatggttga
     5581 ttcttatttc tttgggttga aaacatacaa acactaagca taaaagcaca tgtggtggta
     5641 cctgggtatc tagttcttac ggaataatct caagttggag tttggcgtga agggaaatac
     5701 tggcttaaag tgggataggt atcttttaag cagaaccttg catcttttta aaatttcttg
     5761 agcaaccata catatttgtg aagatttata ttgaacattc tactctgctt aagatctttg
     5821 gttccttcag agcttctcct tagagtatct acctgcatcc ttaataccac gtgatgattc
     5881 atggagcctt gtactgctct tctgagtcta acaaatactg tgactgaaat gcatgcaact
     5941 tgaagagaaa acgttttctt attctccact gtgtctgtga actgtgtagt attccattct
     6001 ttgacagcca tcagtatgtg catgtgtcct cagtaatgac gcagcctttc agtaaccctg
     6061 tgactgccgc atcatagtgg caactttgta tttattagcg agttaaatgt gcaccttggt
     6121 agtgcagtat atagtcttct aagaacagaa gaacattaat tgtatcatca tgaggcagtc
     6181 tagtttgtta gagttggttc attttcactc agctatgtta gccatttttt tctttgtaat
     6241 aaaaagtctc ctttttaaaa agagtcctca agttaatttc aaatcccttc ttggatgcaa
     6301 agtgtgcatt aggccattca ttgaacaaat gactattcgt tgaacaaaat tagctaagca
     6361 tgcttactaa tgctgctccg tatgactgta atgcacatgc ccaaatctcc caggactttt
     6421 tagaccagcc taactgtccc taaaacttcc catggcttat tgatcgctgt ttgatcatcc
     6481 cgtccttatt tcaaatgaga atctaagaaa agcaacatta gaaacccatt cagtatctgt
     6541 ggcaagtgct gccttcaaca aggtgatggc ccctttcagt ctgcctgagc caacaaggga
     6601 aggagagctc agatctgaag tgaattatat ttctcccaca gggagccatt gtctatccca
     6661 ctgttctaag agaccacata accctgcagt cattttatat gcatcatgga tagaggatat
     6721 ttttattctc caatacaaca tcttggcaaa aatattttct gcagcagtaa catttcaaaa
     6781 attctaattg gatgctctga gaagatgaga aagctgtatc cttgtgtgag ccattatctg
     6841 gctggcaggt ttatccttag tcataaatgt cctccttctg ggataagaag tggcagaaga
     6901 caaatgcacc attaagaact gcatctgaat tcgaaattac aaggcagcca gtgcagcaaa
     6961 acttgcttag ctatgctccc agatatgggg catttcttcc agaaatccaa gtgtcagcta
     7021 atgttttctt atgcttggct cacaaaggga gttcctcttg gtggctcatt accaatataa
     7081 tagaaatgct ttttcacagc taagctcttt gaaaaacagg gacagaaaag tagtgtttct
     7141 taatttatta agacaagata tttactcttt tactccttca aatgtgtttg aagactacaa
     7201 atgacaaata ttgaaaatct gcctaattta ttttattttt ttttttttgc ttaaataaag
     7261 ctgttgggca tcaaattgtg agtttagtat gccactattc tctaactcga tgttttagtg
     7321 gatctgagtt gaaccaagaa taccaaataa acacaaccag cagaacagtc tactaaagga
     7381 ctactaaggc tgaaggcaat gctacgattt caaattcttc cttaatgcaa accaaaagtg
     7441 gtatttatca taatgataat ttaagttttg ctcacattat ttcactgtct taatggctta
     7501 aattagggat atttgatagt tctggaaaat aaatggggat gttgtcccag ttagagtaca
     7561 tacagaagaa aacccttcct atcctctctg ccttttcaaa ccaaggcaac catttattcc
     7621 tggttgtctg ctgctgggtg gttttgcatg ctcttcccat ctctgctgta tgcctcttgt
     7681 acaggtgcag gttctgtgta ttccgtgtta atctttacca gtaaatacac ctcacaaggt
     7741 tactttcagt ttggctgaag gagaactgtg cagtaaaatg cgaaggaaag caatgacttt
     7801 attcaatggt tttttttttc atttgaaaca ctattaactt ttaaaataat ttcatagtcc
     7861 cttatacata agccaatgaa atattctgag ttctagttag aaaaaaaaaa gcatgaattc
     7921 tacatgataa atctaaacaa caaaaacact tgtaagttct tttatatgtt tcatgcctta
     7981 ttttccattt ttgacaccat aaagtgcatt ttctgtcaac tgtgagcaaa tttccctttg
     8041 cctttaataa acatagtgca ttaagtagcc agttgctgaa aagttacaaa gcaagttagc
     8101 taaaggtggg tcatactgga ctacattcaa agaaccaaga tacacaaaag atgcaagttg
     8161 tatgtgtgtg gcttagagtg cacctgtgca gatgcatgcc gccttcagta gctgccaaat
     8221 gtgcctttaa ttaaaaacat ctctaatttt ttttttcttc aaatcaagtc agcatttggg
     8281 gacgtgatta gggcaaggga cttgggggta caaatatctg gtgaaatcac cagagaattt
     8341 gtttgtcaaa gtgacatgtg taattttagt tgagatatgt ctgtgtatat atgcgtattt
     8401 gcctttgttt aggagcacgg ttatttgctg aaataattgt taaaaaatgc tgtcagtctt
     8461 actttgatgt aaagaacaaa gtgtggtctg tacatctaat aatgaccttt gtacctaacc
     8521 atgtttgttg gtgatctttg tattctgtgt gcagcaatat ttggtttgtg ttaaagtgaa
     8581 aataatggct gttatttttc tggcattact aagataaatt gaaaaataat aaagaaaaat
     8641 accttatata gcctataagt gtgttatttt gcaagcatta ttttcgaggg cagtacttgg
     8701 aagttgttag tgattctagt ttagcttata tttgaccagg taggttgatc tttgggtata
     8761 cacaggatga cgtttcagag gcagtcacat ttccaaaaca atgagcttag gcggtgcacc
     8821 cagctggggt cgtttgacat gaaagagctt aaggttgtat ctaaagaatt ttgtaaatac
     8881 agtcactcaa aatgtatcat aaaataaata tattaaatg
//
