LOCUS       NM_146064               2126 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus sterol O-acyltransferase 2 (Soat2), mRNA.
ACCESSION   NM_146064 NM_011433
VERSION     NM_146064.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Pramfalk,C., Ahmed,O., Pedrelli,M., Minniti,M.E., Luquet,S.,
            Denis,R.G., Olin,M., Hardfeldt,J., Vedin,L.L., Steffensen,K.R.,
            Ryden,M., Hodson,L., Eriksson,M. and Parini,P.
  TITLE     Soat2 ties cholesterol metabolism to beta-oxidation and glucose
            tolerance in male mice
  JOURNAL   J Intern Med 292 (2), 296-307 (2022)
   PUBMED   34982494
  REMARK    GeneRIF: Soat2 ties cholesterol metabolism to beta-oxidation and
            glucose tolerance in male mice.
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Ramirez,C.M., Taylor,A.M., Lopez,A.M., Repa,J.J. and Turley,S.D.
  TITLE     Delineation of metabolic responses of Npc1-/-nih mice lacking the
            cholesterol-esterifying enzyme SOAT2 to acute treatment with
            2-hydroxypropyl-beta-cyclodextrin
  JOURNAL   Steroids 164, 108725 (2020)
   PUBMED   32890578
  REMARK    GeneRIF: Delineation of metabolic responses of Npc1(-/-nih) mice
            lacking the cholesterol-esterifying enzyme SOAT2 to acute treatment
            with 2-hydroxypropyl-beta-cyclodextrin.
REFERENCE   3  (bases 1 to 2126)
  AUTHORS   Ahmed,O., Pramfalk,C., Pedrelli,M., Olin,M., Steffensen,K.R.,
            Eriksson,M. and Parini,P.
  TITLE     Genetic depletion of Soat2 diminishes hepatic steatosis via genes
            regulating de novo lipogenesis and by GLUT2 protein in female mice
  JOURNAL   Dig Liver Dis 51 (7), 1016-1022 (2019)
   PUBMED   30630736
  REMARK    GeneRIF: The study found that Soat2-/- mice fed high-fat,
            low-cholesterol diet to have less hepatic steatosis, decreased
            expression of genes involved in de novo lipogenesis and lower
            hepatic GLUT2.
REFERENCE   4  (bases 1 to 2126)
  AUTHORS   Lopez,A.M., Jones,R.D., Repa,J.J. and Turley,S.D.
  TITLE     Niemann-Pick C1-deficient mice lacking sterol O-acyltransferase 2
            have less hepatic cholesterol entrapment and improved liver
            function
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 315 (4), G454-G463 (2018)
   PUBMED   29878847
  REMARK    GeneRIF: SOAT2 knockout in NPC1-deficient mice decreases hepatic
            cholesterol accumulation and improves liver function.
REFERENCE   5  (bases 1 to 2126)
  AUTHORS   Wang,Y.J., Bian,Y., Luo,J., Lu,M., Xiong,Y., Guo,S.Y., Yin,H.Y.,
            Lin,X., Li,Q., Chang,C.C.Y., Chang,T.Y., Li,B.L. and Song,B.L.
  TITLE     Cholesterol and fatty acids regulate cysteine ubiquitylation of
            ACAT2 through competitive oxidation
  JOURNAL   Nat Cell Biol 19 (7), 808-819 (2017)
   PUBMED   28604676
  REMARK    GeneRIF: lipid-induced stabilization of ACAT2 ameliorates
            lipotoxicity from excessive cholesterol and fatty acid. This
            unconventional cysteine ubiquitylation of ACAT2 constitutes an
            important mechanism for sensing lipid-overload-induced ROS and
            fine-tuning lipid homeostasis.
            Erratum:[Nat Cell Biol. 2017 Nov 29;19(12):1441. doi:
            10.1038/ncb3651.. PMID: 29184177]
REFERENCE   6  (bases 1 to 2126)
  AUTHORS   Lammert,F., Carey,M.C. and Paigen,B.
  TITLE     Chromosomal organization of candidate genes involved in cholesterol
            gallstone formation: a murine gallstone map
  JOURNAL   Gastroenterology 120 (1), 221-238 (2001)
   PUBMED   11208732
  REMARK    Review article
REFERENCE   7  (bases 1 to 2126)
  AUTHORS   Buhman,K.K., Accad,M., Novak,S., Choi,R.S., Wong,J.S.,
            Hamilton,R.L., Turley,S. and Farese,R.V. Jr.
  TITLE     Resistance to diet-induced hypercholesterolemia and gallstone
            formation in ACAT2-deficient mice
  JOURNAL   Nat Med 6 (12), 1341-1347 (2000)
   PUBMED   11100118
REFERENCE   8  (bases 1 to 2126)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
REFERENCE   9  (bases 1 to 2126)
  AUTHORS   Cases,S., Novak,S., Zheng,Y.W., Myers,H.M., Lear,S.R., Sande,E.,
            Welch,C.B., Lusis,A.J., Spencer,T.A., Krause,B.R., Erickson,S.K.
            and Farese,R.V. Jr.
  TITLE     ACAT-2, a second mammalian acyl-CoA:cholesterol acyltransferase.
            Its cloning, expression, and characterization
  JOURNAL   J Biol Chem 273 (41), 26755-26764 (1998)
   PUBMED   9756919
REFERENCE   10 (bases 1 to 2126)
  AUTHORS   Zhong,J., Edelhoff,S., Disteche,C. and Braun,R.E.
  TITLE     The gene encoding PRBP, the mouse homolog of human TRBP, maps to
            distal chromosome 15
  JOURNAL   Mamm Genome 9 (5), 413-414 (1998)
   PUBMED   9545508
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC025931.1.
            
            On Mar 16, 2004 this sequence version replaced NM_011433.1.
            
            Sequence Note: This sequence has been modified as follows: removed
            44 bp suspected to be vector contamination from the 3' end.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025931.1, AF078751.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2126              BC025931.1         1-2126
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="FVB/N"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 57.33 cM"
     gene            1..2126
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /note="sterol O-acyltransferase 2"
                     /db_xref="GeneID:223920"
                     /db_xref="MGI:MGI:1332226"
     exon            1..138
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     CDS             60..1637
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /EC_number="2.3.1.26"
                     /note="cholesterol acyltransferase 2; acyl-CoA:cholesterol
                     acyltransferase; ACAT-2; acyl-coenzyme A:cholesterol
                     acyltransferase 2"
                     /codon_start=1
                     /product="sterol O-acyltransferase 2"
                     /protein_id="NP_666176.1"
                     /db_xref="CCDS:CCDS27872.1"
                     /db_xref="GeneID:223920"
                     /db_xref="MGI:MGI:1332226"
                     /translation="MQPKVPQLRRREGLGEEQEKGARGGEGNARTHGTPDLVQWTRHM
                     EAVKTQFLEQAQRELAELLDRALWEAMQAYPKQDRPLPSAAPDSTSKTQELHPGKRKV
                     FITRKSLIDELMEVQHFRTIYHMFIAGLCVLIISTLAIDFIDEGRLMLEFDLLLFSFG
                     QLPLALMTWVPMFLSTLLVPYQTLWLWARPRAGGAWMLGASLGCVLLAAHAVVLCVLP
                     VHVSVRHELPPASRCVLVFEQVRLLMKSYSFLRETVPGIFCVRGGKGISPPSFSSYLY
                     FLFCPTLIYRETYPRTPSIRWNYVAKNFAQVLGCLLYACFILGRLCVPVFANMSREPF
                     STRALLLSILHATGPGIFMLLLIFFAFLHCWLNAFAEMLRFGDRMFYRDWWNSTSFSN
                     YYRTWNVVVHDWLYSYVYQDGLWLLGRRARGVAMLGVFLVSAVVHEYIFCFVLGFFYP
                     VMLMLFLVFGGLLNFTMNDRHTGPAWNILMWTFLFMGQGIQVSLYCQEWYARRHCPLP
                     QTTFWGMVTPRSWSCHP"
     exon            139..194
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            195..331
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            332..391
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            392..499
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            500..776
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            777..846
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            847..931
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            932..977
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1107
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            1108..1205
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            1206..1304
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            1305..1440
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1586
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
     exon            1587..2126
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /inference="alignment:Splign:2.1.0"
ORIGIN      
        1 aaagaaagaa aggaagaaga cagacaacag ctgtgtgctg tccgctctac actggcacca
       61 tgcagccaaa ggtgccccag cttcggagga gagaagggct gggagaggag caggagaagg
      121 gagcccgtgg aggagaaggg aacgcaagga cacacggaac cccagacttg gtgcaatgga
      181 ctcgacatat ggaggctgtg aagacacagt ttctggagca agcacagaga gagttggcag
      241 agctgttgga tcgggcccta tgggaggcta tgcaagctta ccccaaacaa gacagacctc
      301 ttccctccgc tgccccagat tctacaagca agacccaaga gttacaccct ggaaaacgga
      361 aagttttcat cacccgcaag tcactgatcg atgagctaat ggaggtgcaa catttccgaa
      421 ccatctacca catgttcata gcgggcctat gcgtcctgat catcagcacc ctggccatcg
      481 acttcattga tgagggcagg ttgatgctgg agtttgactt actcctcttc agcttcggac
      541 agctgccctt ggcgctgatg acctgggttc ccatgttcct gtctacgctc ctagtgccct
      601 accagaccct gtggctgtgg gccaggccgc gcgctggggg tgcctggatg ctgggggcca
      661 gcctgggctg cgttctgctg gctgcccacg ctgtggtgct ctgcgtcctg ccggtgcacg
      721 tgtcagtgag gcatgagctt ccgcccgcct cgcgctgcgt gctggtcttt gagcaggtca
      781 gattgctgat gaaaagctac tccttcctga gagagactgt gcctgggatc ttttgtgtca
      841 gaggaggaaa gggcatcagc cccccaagtt tctccagcta cctctacttc ctcttctgcc
      901 ctacacttat ctacagagag acatacccca ggacacccag catcaggtgg aactatgtgg
      961 ccaagaactt tgcccaggtc ctgggctgtt tgctctatgc ctgcttcatc ctgggccgcc
     1021 tctgtgtccc tgtctttgcc aacatgagcc gggaaccctt cagcacccgg gctctgctgc
     1081 tctccatctt gcatgccacg gggccaggca tcttcatgct gctcctcatc ttcttcgcct
     1141 tcctgcactg ctggctcaac gccttcgccg agatgctgcg gtttggagac aggatgttct
     1201 accgggactg gtggaactcg acttccttct ccaactacta ccgcacctgg aacgtcgtgg
     1261 tccatgactg gctgtacagc tatgtgtatc aagatgggct gtggctctta ggcaggcggg
     1321 ctcgcggggt ggccatgctg ggagtgttcc tggtgtctgc ggtggttcat gagtatatct
     1381 tctgcttcgt cctggggttc ttctacccgg tcatgctgat gctattcctt gttttcgggg
     1441 ggctgctgaa tttcaccatg aacgacaggc acacaggtcc agcctggaac atcctgatgt
     1501 ggacctttct cttcatgggc cagggcatcc aggtcagcct atactgccag gagtggtatg
     1561 ctcgtcgaca ctgtcccctg ccccagacaa cattctgggg gatggtgaca ccccgctctt
     1621 ggtcctgcca tccctagaag tcaaggcttc atgctacaca gatgacagca tcaagctctt
     1681 ctctgcctgc aaagcccaga gccagggttc ctcactacct catttggagt cagggacttg
     1741 cacataggac cttccagggg gccatgggga ctcaggtacc cacctctgtg gtcagctgac
     1801 cacaggctca gtatggtgtg actcgagcac ctaccctctg tggactgggc acaggttccc
     1861 ttcatgtctc tccaagtgtg cggagactca gaggaacagg gcacaccaaa gtctgacacg
     1921 tttggttctt tctgcttctc ccaatgcaac aataaaaatc ttagtctcgg actggagaga
     1981 tggctcagtg gttaagagca ctgagtgctc ttccagaggt cctgagttca attcccagca
     2041 accacatggt ggctcacagc catctgaaat gggatctgat gccctcttct ggtgtgtctg
     2101 aagacagcga cagtgtactc acatac
//
