LOCUS       NP_666176                525 aa            linear   ROD 10-FEB-2025
DEFINITION  sterol O-acyltransferase 2 [Mus musculus].
ACCESSION   NP_666176 NP_035563
VERSION     NP_666176.1
DBSOURCE    REFSEQ: accession NM_146064.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 525)
  AUTHORS   Pramfalk,C., Ahmed,O., Pedrelli,M., Minniti,M.E., Luquet,S.,
            Denis,R.G., Olin,M., Hardfeldt,J., Vedin,L.L., Steffensen,K.R.,
            Ryden,M., Hodson,L., Eriksson,M. and Parini,P.
  TITLE     Soat2 ties cholesterol metabolism to beta-oxidation and glucose
            tolerance in male mice
  JOURNAL   J Intern Med 292 (2), 296-307 (2022)
   PUBMED   34982494
  REMARK    GeneRIF: Soat2 ties cholesterol metabolism to beta-oxidation and
            glucose tolerance in male mice.
REFERENCE   2  (residues 1 to 525)
  AUTHORS   Ramirez,C.M., Taylor,A.M., Lopez,A.M., Repa,J.J. and Turley,S.D.
  TITLE     Delineation of metabolic responses of Npc1-/-nih mice lacking the
            cholesterol-esterifying enzyme SOAT2 to acute treatment with
            2-hydroxypropyl-beta-cyclodextrin
  JOURNAL   Steroids 164, 108725 (2020)
   PUBMED   32890578
  REMARK    GeneRIF: Delineation of metabolic responses of Npc1(-/-nih) mice
            lacking the cholesterol-esterifying enzyme SOAT2 to acute treatment
            with 2-hydroxypropyl-beta-cyclodextrin.
REFERENCE   3  (residues 1 to 525)
  AUTHORS   Ahmed,O., Pramfalk,C., Pedrelli,M., Olin,M., Steffensen,K.R.,
            Eriksson,M. and Parini,P.
  TITLE     Genetic depletion of Soat2 diminishes hepatic steatosis via genes
            regulating de novo lipogenesis and by GLUT2 protein in female mice
  JOURNAL   Dig Liver Dis 51 (7), 1016-1022 (2019)
   PUBMED   30630736
  REMARK    GeneRIF: The study found that Soat2-/- mice fed high-fat,
            low-cholesterol diet to have less hepatic steatosis, decreased
            expression of genes involved in de novo lipogenesis and lower
            hepatic GLUT2.
REFERENCE   4  (residues 1 to 525)
  AUTHORS   Lopez,A.M., Jones,R.D., Repa,J.J. and Turley,S.D.
  TITLE     Niemann-Pick C1-deficient mice lacking sterol O-acyltransferase 2
            have less hepatic cholesterol entrapment and improved liver
            function
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 315 (4), G454-G463 (2018)
   PUBMED   29878847
  REMARK    GeneRIF: SOAT2 knockout in NPC1-deficient mice decreases hepatic
            cholesterol accumulation and improves liver function.
REFERENCE   5  (residues 1 to 525)
  AUTHORS   Wang,Y.J., Bian,Y., Luo,J., Lu,M., Xiong,Y., Guo,S.Y., Yin,H.Y.,
            Lin,X., Li,Q., Chang,C.C.Y., Chang,T.Y., Li,B.L. and Song,B.L.
  TITLE     Cholesterol and fatty acids regulate cysteine ubiquitylation of
            ACAT2 through competitive oxidation
  JOURNAL   Nat Cell Biol 19 (7), 808-819 (2017)
   PUBMED   28604676
  REMARK    GeneRIF: lipid-induced stabilization of ACAT2 ameliorates
            lipotoxicity from excessive cholesterol and fatty acid. This
            unconventional cysteine ubiquitylation of ACAT2 constitutes an
            important mechanism for sensing lipid-overload-induced ROS and
            fine-tuning lipid homeostasis.
            Erratum:[Nat Cell Biol. 2017 Nov 29;19(12):1441. doi:
            10.1038/ncb3651.. PMID: 29184177]
REFERENCE   6  (residues 1 to 525)
  AUTHORS   Lammert,F., Carey,M.C. and Paigen,B.
  TITLE     Chromosomal organization of candidate genes involved in cholesterol
            gallstone formation: a murine gallstone map
  JOURNAL   Gastroenterology 120 (1), 221-238 (2001)
   PUBMED   11208732
  REMARK    Review article
REFERENCE   7  (residues 1 to 525)
  AUTHORS   Buhman,K.K., Accad,M., Novak,S., Choi,R.S., Wong,J.S.,
            Hamilton,R.L., Turley,S. and Farese,R.V. Jr.
  TITLE     Resistance to diet-induced hypercholesterolemia and gallstone
            formation in ACAT2-deficient mice
  JOURNAL   Nat Med 6 (12), 1341-1347 (2000)
   PUBMED   11100118
REFERENCE   8  (residues 1 to 525)
  AUTHORS   Ko,M.S., Threat,T.A., Wang,X., Horton,J.H., Cui,Y., Wang,X.,
            Pryor,E., Paris,J., Wells-Smith,J., Kitchen,J.R., Rowe,L.B.,
            Eppig,J., Satoh,T., Brant,L., Fujiwara,H., Yotsumoto,S. and
            Nakashima,H.
  TITLE     Genome-wide mapping of unselected transcripts from extraembryonic
            tissue of 7.5-day mouse embryos reveals enrichment in the t-complex
            and under-representation on the X chromosome
  JOURNAL   Hum Mol Genet 7 (12), 1967-1978 (1998)
   PUBMED   9811942
REFERENCE   9  (residues 1 to 525)
  AUTHORS   Cases,S., Novak,S., Zheng,Y.W., Myers,H.M., Lear,S.R., Sande,E.,
            Welch,C.B., Lusis,A.J., Spencer,T.A., Krause,B.R., Erickson,S.K.
            and Farese,R.V. Jr.
  TITLE     ACAT-2, a second mammalian acyl-CoA:cholesterol acyltransferase.
            Its cloning, expression, and characterization
  JOURNAL   J Biol Chem 273 (41), 26755-26764 (1998)
   PUBMED   9756919
REFERENCE   10 (residues 1 to 525)
  AUTHORS   Zhong,J., Edelhoff,S., Disteche,C. and Braun,R.E.
  TITLE     The gene encoding PRBP, the mouse homolog of human TRBP, maps to
            distal chromosome 15
  JOURNAL   Mamm Genome 9 (5), 413-414 (1998)
   PUBMED   9545508
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from BC025931.1.
            
            On Mar 16, 2004 this sequence version replaced NP_035563.1.
            
            Sequence Note: This sequence has been modified as follows: removed
            44 bp suspected to be vector contamination from the 3' end.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC025931.1, AF078751.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..525
                     /organism="Mus musculus"
                     /strain="FVB/N"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 57.33 cM"
     Protein         1..525
                     /product="sterol O-acyltransferase 2"
                     /EC_number="2.3.1.26"
                     /note="cholesterol acyltransferase 2; acyl-CoA:cholesterol
                     acyltransferase; ACAT-2; acyl-coenzyme A:cholesterol
                     acyltransferase 2"
                     /calculated_mol_wt=60466
     Region          1..34
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Region          77..97
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Site            120..141
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Site            162..187
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Site            200..223
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Site            232..255
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Region          <263..497
                     /region_name="MBOAT"
                     /note="membrane-bound O-acyltransferase family; pfam03062"
                     /db_xref="CDD:427117"
     Site            297..329
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Site            347..372
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Region          380..386
                     /region_name="FYXDWWN motif.
                     /evidence=ECO:0000250|UniProtKB:P35610"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Site            421..445
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Site            452..467
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     Site            474..505
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (O88908.2)"
     CDS             1..525
                     /gene="Soat2"
                     /gene_synonym="ACAT2; D15Wsu97e"
                     /coded_by="NM_146064.1:60..1637"
                     /db_xref="CCDS:CCDS27872.1"
                     /db_xref="GeneID:223920"
                     /db_xref="MGI:MGI:1332226"
ORIGIN      
        1 mqpkvpqlrr reglgeeqek garggegnar thgtpdlvqw trhmeavktq fleqaqrela
       61 elldralwea mqaypkqdrp lpsaapdsts ktqelhpgkr kvfitrksli delmevqhfr
      121 tiyhmfiagl cvliistlai dfidegrlml efdlllfsfg qlplalmtwv pmflstllvp
      181 yqtlwlwarp raggawmlga slgcvllaah avvlcvlpvh vsvrhelppa srcvlvfeqv
      241 rllmksysfl retvpgifcv rggkgispps fssylyflfc ptliyretyp rtpsirwnyv
      301 aknfaqvlgc llyacfilgr lcvpvfanms repfstrall lsilhatgpg ifmllliffa
      361 flhcwlnafa emlrfgdrmf yrdwwnstsf snyyrtwnvv vhdwlysyvy qdglwllgrr
      421 argvamlgvf lvsavvheyi fcfvlgffyp vmlmlflvfg gllnftmndr htgpawnilm
      481 wtflfmgqgi qvslycqewy arrhcplpqt tfwgmvtprs wschp
//
