LOCUS       NM_009230               4785 bp    mRNA    linear   ROD 15-JUL-2024
DEFINITION  Mus musculus sterol O-acyltransferase 1 (Soat1), mRNA.
ACCESSION   NM_009230
VERSION     NM_009230.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4785)
  AUTHORS   Bairos,J.A., Njoku,U., Zafar,M., Akl,M.G., Li,L., Parlakgul,G.,
            Arruda,A.P. and Widenmaier,S.B.
  TITLE     Sterol O-acyltransferase (SOAT/ACAT) activity is required to form
            cholesterol crystals in hepatocyte lipid droplets
  JOURNAL   Biochim Biophys Acta Mol Cell Biol Lipids 1869 (6), 159512 (2024)
   PUBMED   38761895
  REMARK    GeneRIF: Sterol O-acyltransferase (SOAT/ACAT) activity is required
            to form cholesterol crystals in hepatocyte lipid droplets.
REFERENCE   2  (bases 1 to 4785)
  AUTHORS   Wilkerson,A., Yuksel,S., Acharya,R. and Butovich,I.A.
  TITLE     Physiological Effects of Soat1 Inactivation on Homeostasis of the
            Mouse Ocular Surface
  JOURNAL   Invest Ophthalmol Vis Sci 65 (8), 2 (2024)
   PUBMED   38953847
  REMARK    GeneRIF: Physiological Effects of Soat1 Inactivation on Homeostasis
            of the Mouse Ocular Surface.
REFERENCE   3  (bases 1 to 4785)
  AUTHORS   Dearden,L., Furigo,I.C., Pantaleao,L.C., Wong,L.W.P.,
            Fernandez-Twinn,D.S., de Almeida-Faria,J., Kentistou,K.A.,
            Carreira,M.V., Bidault,G., Vidal-Puig,A., Ong,K.K., Perry,J.R.B.,
            Donato,J. Jr. and Ozanne,S.E.
  TITLE     Maternal obesity increases hypothalamic miR-505-5p expression in
            mouse offspring leading to altered fatty acid sensing and increased
            intake of high-fat food
  JOURNAL   PLoS Biol 22 (6), e3002641 (2024)
   PUBMED   38833481
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4785)
  AUTHORS   Xu,X., Wilkerson,A., Li,G., Butovich,I.A. and Zuo,Y.Y.
  TITLE     Comparative Biophysical Study of Meibomian Lipids of Wild Type and
            Soat1-Null Mice: Implications to Meibomian Gland Dysfunction and
            Dry Eye Disease
  JOURNAL   Invest Ophthalmol Vis Sci 64 (11), 20 (2023)
   PUBMED   37585190
  REMARK    GeneRIF: Comparative Biophysical Study of Meibomian Lipids of Wild
            Type and Soat1-Null Mice: Implications to Meibomian Gland
            Dysfunction and Dry Eye Disease.
REFERENCE   5  (bases 1 to 4785)
  AUTHORS   Zaidi,S.A.H., Lemtalsi,T., Xu,Z., Santana,I., Sandow,P., Labazi,L.,
            Caldwell,R.W., Caldwell,R.B. and Rojas,M.A.
  TITLE     Role of acyl-coenzyme A: cholesterol transferase 1 (ACAT1) in
            retinal neovascularization
  JOURNAL   J Neuroinflammation 20 (1), 14 (2023)
   PUBMED   36691048
  REMARK    GeneRIF: Role of acyl-coenzyme A: cholesterol transferase 1 (ACAT1)
            in retinal neovascularization.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4785)
  AUTHORS   Gessler,M., Hameister,H., Henry,I., Junien,C., Braun,T. and
            Arnold,H.H.
  TITLE     The human MyoD1 (MYF3) gene maps on the short arm of chromosome 11
            but is not associated with the WAGR locus or the region for the
            Beckwith-Wiedemann syndrome
  JOURNAL   Hum Genet 86 (2), 135-138 (1990)
   PUBMED   2176177
REFERENCE   7  (bases 1 to 4785)
  AUTHORS   Elliott,R.W., Barlow,D. and Hogan,B.L.
  TITLE     Linkage of genes for laminin B1 and B2 subunits on chromosome 1 in
            mouse
  JOURNAL   In Vitro Cell Dev Biol 21 (8), 477-484 (1985)
   PUBMED   2993224
REFERENCE   8  (bases 1 to 4785)
  AUTHORS   Wilcox,F.H., Hirschhorn,L., Taylor,B.A., Womack,J.E. and
            Roderick,T.H.
  TITLE     Genetic variation in alkaline phosphatase of the house mouse (Mus
            musculus) with emphasis on a manganese-requiring isozyme
  JOURNAL   Biochem Genet 17 (11-12), 1093-1107 (1979)
   PUBMED   540002
REFERENCE   9  (bases 1 to 4785)
  AUTHORS   Wilson,C.M., Erdos,E.G., Wilson,J.D. and Taylor,B.A.
  TITLE     Location on chromosome 1 of Rnr, a gene that regulates renin in the
            submaxillary gland of the mouse
  JOURNAL   Proc Natl Acad Sci U S A 75 (11), 5623-5626 (1978)
   PUBMED   281712
REFERENCE   10 (bases 1 to 4785)
  AUTHORS   Taylor,B.A. and Meier,H.
  TITLE     Mapping the adrenal lipid depletion gene of the AKR/J mouse strain
  JOURNAL   Genet Res 26 (3), 307-312 (1975)
   PUBMED   1225763
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ164331.1, BC083092.1 and AC161221.7.
            
            On Aug 16, 2006 this sequence version replaced NM_009230.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK142486.1, DQ903181.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164134, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-198               CJ164331.1         4-201
            199-2299            BC083092.1         13-2113
            2300-4785           AC161221.7         46799-49284
FEATURES             Location/Qualifiers
     source          1..4785
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 67.71 cM"
     gene            1..4785
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /note="sterol O-acyltransferase 1"
                     /db_xref="GeneID:20652"
                     /db_xref="MGI:MGI:104665"
     exon            1..181
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            182..299
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     CDS             212..1834
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /EC_number="2.3.1.26"
                     /note="cholesterol acyltransferase 1; acyl-coenzyme
                     A:cholesterol acyltransferase 1; adrenocortical lipid
                     depletion"
                     /codon_start=1
                     /product="sterol O-acyltransferase 1"
                     /protein_id="NP_033256.2"
                     /db_xref="CCDS:CCDS35743.1"
                     /db_xref="GeneID:20652"
                     /db_xref="MGI:MGI:104665"
                     /translation="MSLRNRLSKSGENPEQDEAQKNFMDTYRNGHITMKQLIAKKRLL
                     AAEAEELKPLFMKEVGCHFDDFVTNLIEKSASLDNGGCALTTFSILEEMKKNHRAKDL
                     RAPPEQGKIFISRQSLLDELFEVDHIRTIYHMFIALLILFVLSTIVVDYIDEGRLVLE
                     FNLLAYAFGKFPTVIWTWWAMFLSTLSIPYFLFQRWAHGYSKSSHPLIYSLVHGLLFL
                     VFQLGVLGFVPTYVVLAYTLPPASRFILILEQIRLIMKAHSFVRENIPRVLNAAKEKS
                     SKDPLPTVNQYLYFLFAPTLIYRDNYPRTPTVRWGYVAMQFLQVFGCLFYVYYIFERL
                     CAPLFRNIKQEPFSARVLVLCVFNSILPGVLILFLSFFAFLHCWLNAFAEMLRFGDRM
                     FYKDWWNSTSYSNYYRTWNVVVHDWLYYYVYKDLLWFFSKRFKSAAMLAVFALSAVVH
                     EYALAICLSYFYPVLFVLFMFFGMAFNFIVNDSRKRPIWNIMVWASLFLGYGLILCFY
                     SQEWYARQHCPLKNPTFLDYVRPRTWTCRYVF"
     exon            300..358
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            359..510
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            511..570
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            571..678
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            679..961
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            962..1040
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            1041..1122
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            1123..1168
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            1169..1298
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1396
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            1397..1495
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            1496..1631
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            1632..1777
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     exon            1778..4785
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3076..3081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /note="hexamer: AATAAA"
     polyA_site      3102
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /note="major polyA site"
     regulatory      4390..4395
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /note="hexamer: AATAAA"
     polyA_site      4411
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
     regulatory      4574..4579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
                     /note="hexamer: AAGAAA"
     polyA_site      4598
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
     polyA_site      4782
                     /gene="Soat1"
                     /gene_synonym="8430426K15Rik; Acact; ACAT-1; ald; hid"
ORIGIN      
        1 gcgtgacgca gccgcgggct gggaggttgg ggcggagaac gggggtgggg gcctgcgctt
       61 tatcttcctc cgtagcccgg ctggcggcgt agcgggccgg cgaagggaca tcctagtctc
      121 cgaccgtcct gcggccgccc agcctaccgc ctagaccgcc gcagctgccc tccgctcgca
      181 ggaagcttga ttgatagtgg gagaagagaa gatgtcacta agaaaccggc tgtcaaaatc
      241 tggggagaat cctgagcaag atgaagccca gaaaaatttc atggacacat acagaaatgg
      301 tcacattacc atgaaacaat tgatagccaa gaagaggctg ttggcagcag aggcggagga
      361 gctgaagcca ctatttatga aggaagttgg gtgccacttc gatgactttg tgaccaacct
      421 cattgaaaag tccgcatcgc tggacaatgg tgggtgtgca ctcacaacct tctccattct
      481 tgaagaaatg aaaaagaacc acagagccaa agatctgaga gcacctccag aacaagggaa
      541 gatttttatt tcaaggcagt ctcttttaga cgagctgttt gaagtggacc acatcagaac
      601 aatttaccac atgttcatcg cactcctcat cctattcgtc ctcagcacga tcgtcgtgga
      661 ctacatcgat gaaggaaggc tggtacttga gttcaatctc ctggcctatg cttttggcaa
      721 atttcctact gttatttgga catggtgggc catgttcctg tctacactgt caatccccta
      781 tttcctgttc cagcgatggg cccacggtta cagcaagagt tctcacccat tgatctattc
      841 ccttgtccat ggcttgctct tcttagtctt tcaacttgga gttctaggtt ttgtgccaac
      901 atacgttgtc ttagcataca cactgccacc agcctcccgg ttcattctga tactggaaca
      961 gattcgtttg ataatgaagg ctcactcatt tgtcagagag aatatcccac gagtactaaa
     1021 tgcagccaag gagaaatcaa gcaaagatcc actacccaca gtcaaccagt acctgtactt
     1081 cctgtttgcg cctacactta tttaccgaga caactaccca aggactccta ctgtaagatg
     1141 gggttatgtc gctatgcagt ttttacaggt gtttggctgc ctgttttatg tgtactacat
     1201 ctttgagaga ctctgtgccc cactattccg gaatatcaaa caggagccct tcagtgctcg
     1261 tgtcctggtc ctgtgtgtgt ttaactccat cttgccaggt gtcttgatac tgttcctttc
     1321 gttctttgcc tttttgcact gctggctcaa tgcctttgct gagatgttac gctttggtga
     1381 caggatgttt tataaggact ggtggaactc tacatcatac tccaactact acaggacctg
     1441 gaacgtggtg gtgcacgact ggctctacta ctatgtttac aaagacctgc tctggttttt
     1501 ctcgaagagg ttcaaatctg ccgccatgct ggccgtcttc gccctgtcgg ctgtggtgca
     1561 cgagtatgcc ctcgccatct gcctgagtta cttctacccg gtgctcttcg tgctcttcat
     1621 gttctttgga atggctttta acttcattgt taacgacagt cggaaaaggc caatctggaa
     1681 catcatggtt tgggcttctc tgttcctggg ctatggactc attctgtgct tttattctca
     1741 agagtggtat gcccgccagc actgtcctct gaagaaccct acatttctgg attatgtccg
     1801 gccgcggacc tggacttgtc ggtacgtgtt ttagaagccc tggctctgcc tccttgcttg
     1861 ccattgaaga ttggatattc ttcctgattc cgagccagcc gtcttcagcg gttactgaat
     1921 ccccacactg cggtgtttgg ggctctctat cctttctgat gattcacctg gagttgggct
     1981 gtgggaagcc cacttcgtga tttttttgtt ttgttttgtt ttgttccctt tgggggagaa
     2041 aggagactga cagttgagat aatggcagat ttttgcaggg ttatctcagg tcaagccttg
     2101 gtagttttat ctgtttattt ccttgattcc atccaaccag agactaaaca tatcttgacc
     2161 atgagttagc caaaacactt aggaagacat catttctgta tctctgagca tagaagtttt
     2221 gttcatgcac attatcaaga ggtatgctag tgcaattgag aagaaagaaa ttttgttatt
     2281 tttggtagag aaaaaaaaaa ccctattttc caaagataat cttgttatgc attctgctcg
     2341 tctcttttgt tgctattctg ctttgctttg cttctgtgct aggagtggaa cccagggtct
     2401 cgctcaggga cagcaccctg agccacagcc caggccttcc actgtatttt tctgaaagtt
     2461 ctggtctact gttttgtttt ccattttctg tataagttgt gattctttta aatgattagt
     2521 gttccccttg aactcaatgc tagtacaaat tcaagggtca gaagtagaaa gggaaaaaag
     2581 acatttcctt ttgaaatttc aaaagtcatt ttaatacaga ctcactgagc tgcccatgtg
     2641 tttttaattg tgctaccata gaagttctgt ggaagatttt attattgttg gtttgatttt
     2701 ggttttttag aggtggggtc ttgctatgta gcccaggctg gtcttgaact tgctatgtag
     2761 cctgactggt cttgaacccc tgacctcctg tgtcccaagt gctaggatca cagaagacat
     2821 ctccatgcct ggccaagatt tttttttttt tttttttaac ttttaggtta taacttcttt
     2881 gaagttactt agaagtatcc tttggtctaa ttgctttatt gtctaatgcg tagtgacttt
     2941 ccttgttata gactagcgag tccatagtgg aagtattaat ctttctctac taataatccc
     3001 aaaatggctc ctaaggctcc agaagctggc ttctgaaaca tctgctcagt cattatactt
     3061 tatattttta gatgaaataa atgactatgt gatcatttgt aaggacatgt actcatatgt
     3121 gacatttgca aattgtgagg cgctcattcc tcacctggtg tgtcagaagg acctgctgtg
     3181 cttgtgtgca agggtgagcc tatgtgtgcg aggactgtgc atcagaaagg taccacggac
     3241 cacagagttc aaaccttctg cccaaagaaa tgagccgtac aagttgctca cacacaaaat
     3301 cccatggcac agtcactttg gtggtttcct ggcaaccatc cgtacagttt tgggaagtct
     3361 tttcagagtg gctttaaaaa tcacttttga gtctcacgag atctggaggg ctcttttata
     3421 atcactctcc gctaggacca caggtctcca ggttccttac ctacctattg accactgctg
     3481 gggatgaata gtggctgttt ctgtcagttc tttgttccca aacaatagtg ttctgtgttg
     3541 tcacagaacc atagcccctt gataagtctg taaaggcttg tgtgtgttgt tttgttttgt
     3601 tttgtttttt tgtttttcga gacggtttct ctgtgtagcc ctgtctgtcc tggaactcac
     3661 tttgtagacc aggctggcct cgaactcaga aatccacctg cctctgcctc ccaagtgctg
     3721 ggattaaagg cgtacgccac caccgccctg ctaaggctgt gacttaaaga ttaaaacaag
     3781 ctggttactt ttgtgtgtaa tggcatttta aatataatga gggaacagac cggccaggga
     3841 cttggttata agccaggctt ctgaagcaag gggatgggat ttcagaggtg ggggtggggg
     3901 taagggtggg gatgggggtg tccctttctt tgaagtacta gatatgcatt tttaggaaag
     3961 ggcattactt tgtagcagtt ctccattcca atgttggact gatcatggcc acccttcccc
     4021 ctcacccact cttttttgag acaaggtctt tctctgcaac caagctagtg tggacctcac
     4081 atcctcctgc ctcagcctcc tcaggtgcca gagtttataa tataagcatg tgctgcctca
     4141 gaaggccttt cattaggaat tcttttcctg tcaagatttt agatgataat aatgtgtgta
     4201 agactgcaaa ctacttagca ttcaaagagc gtcttaggac aacctcaaaa atacaaaacc
     4261 ccaaacgagg tggccacttc tgtgaaagaa gattgggttg gtaaccatga ccacgttggc
     4321 cgcattcgca ggagtctttt tttttttttt tttttttttt caaaccatgt ctgaactctt
     4381 taaagagaaa ataaaaccaa acctcttgct agcagtgtgt tccaagctgg tttgtttatt
     4441 gtttattatt ccgtgttgtg ccaatgggag aaagctgttt tctcttgagg atacttctta
     4501 tttgtctaca aagtaagggg tttagtgttt tctataagaa gtattaatgc tgataactga
     4561 ttttaagtga cccaagaaaa ctactgtctc tacagccatt tcacgttgta tttggatgtc
     4621 tgcttcggcc ttgtgcgact tatgtgttgg acatttttat tctcagtctc agataagagg
     4681 gagagggagc aagtttaggg tttttttttg ttgatggatg aatgtattaa aattacctgg
     4741 gtaacattgc ttgcctcggg ttagacttta ttagaacact aagac
//
