LOCUS       NM_009224               1684 bp    mRNA    linear   ROD 13-FEB-2025
DEFINITION  Mus musculus small nuclear ribonucleoprotein 70 (U1) (Snrnp70),
            transcript variant 1, mRNA.
ACCESSION   NM_009224
VERSION     NM_009224.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 1684)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 1684)
  AUTHORS   Feng,S., Li,J., Wen,H., Liu,K., Gui,Y., Wen,Y., Wang,X. and Yuan,S.
  TITLE     hnRNPH1 recruits PTBP2 and SRSF3 to modulate alternative splicing
            in germ cells
  JOURNAL   Nat Commun 13 (1), 3588 (2022)
   PUBMED   35739118
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 1684)
  AUTHORS   Nakaya,T.
  TITLE     Dissection of FUS domains involved in regulation of SnRNP70 gene
            expression
  JOURNAL   FEBS Lett 594 (21), 3518-3529 (2020)
   PUBMED   32915994
  REMARK    GeneRIF: Dissection of FUS domains involved in regulation of
            SnRNP70 gene expression.
REFERENCE   4  (bases 1 to 1684)
  AUTHORS   Subramania,S., Gagne,L.M., Campagne,S., Fort,V., O'Sullivan,J.,
            Mocaer,K., Feldmuller,M., Masson,J.Y., Allain,F.H.T., Hussein,S.M.
            and Huot,M.E.
  TITLE     SAM68 interaction with U1A modulates U1 snRNP recruitment and
            regulates mTor pre-mRNA splicing
  JOURNAL   Nucleic Acids Res 47 (8), 4181-4197 (2019)
   PUBMED   30767021
  REMARK    GeneRIF: SAM68 interaction with U1A modulates U1 snRNP recruitment
            and regulates mTor pre-mRNA splicing.
REFERENCE   5  (bases 1 to 1684)
  AUTHORS   Donadon,I., Pinotti,M., Rajkowska,K., Pianigiani,G., Barbon,E.,
            Morini,E., Motaln,H., Rogelj,B., Mingozzi,F., Slaugenhaupt,S.A. and
            Pagani,F.
  TITLE     Exon-specific U1 snRNAs improve ELP1 exon 20 definition and rescue
            ELP1 protein expression in a familial dysautonomia mouse model
  JOURNAL   Hum Mol Genet 27 (14), 2466-2476 (2018)
   PUBMED   29701768
  REMARK    GeneRIF: This splice-switching class of molecules is the first to
            specifically correct the ELP1 exon 20 splicing defect. Our data
            provide proof of principle of ExSpeU1s-adeno-associated virus
            particles as a novel therapeutic strategy for FD.
            Erratum:[Hum Mol Genet. 2024 Apr 18;33(9):836. doi:
            10.1093/hmg/ddae017.. PMID: 38511726]
REFERENCE   6  (bases 1 to 1684)
  AUTHORS   Greidinger,E.L., Zang,Y.J., Jaimes,K., Martinez,L., Nassiri,M. and
            Hoffman,R.W.
  TITLE     CD4+ T cells target epitopes residing within the RNA-binding domain
            of the U1-70-kDa small nuclear ribonucleoprotein autoantigen and
            have restricted TCR diversity in an HLA-DR4-transgenic murine model
            of mixed connective tissue disease
  JOURNAL   J Immunol 180 (12), 8444-8454 (2008)
   PUBMED   18523312
REFERENCE   7  (bases 1 to 1684)
  AUTHORS   Howell,V.M., Jones,J.M., Bergren,S.K., Li,L., Billi,A.C.,
            Avenarius,M.R. and Meisler,M.H.
  TITLE     Evidence for a direct role of the disease modifier SCNM1 in
            splicing
  JOURNAL   Hum Mol Genet 16 (20), 2506-2516 (2007)
   PUBMED   17656373
REFERENCE   8  (bases 1 to 1684)
  AUTHORS   Pelsue,S. and Agris,P.F.
  TITLE     Gene usage by an anti-U1 snRNP 70K monoclonal autoantibody derived
            from a lupus-prone mouse
  JOURNAL   J Autoimmun 7 (2), 165-177 (1994)
   PUBMED   8037837
REFERENCE   9  (bases 1 to 1684)
  AUTHORS   Hornig,H., Fischer,U., Costas,M., Rauh,A. and Luhrmann,R.
  TITLE     Analysis of genomic clones of the murine U1RNA-associated 70-kDa
            protein reveals a high evolutionary conservation of the protein
            between human and mouse
  JOURNAL   Eur J Biochem 182 (1), 45-50 (1989)
   PUBMED   2525092
REFERENCE   10 (bases 1 to 1684)
  AUTHORS   Query,C.C. and Keene,J.D.
  TITLE     A human autoimmune protein associated with U1 RNA contains a region
            of homology that is cross-reactive with retroviral p30gag antigen
  JOURNAL   Cell 51 (2), 211-220 (1987)
   PUBMED   2959371
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151602.4.
            
            On Jan 12, 2022 this sequence version replaced NM_009224.5.
            
            Summary: This gene encodes a subunit of the U1 snRNP (small nuclear
            ribonucleic particle), one of at least five snRNPs to comprise the
            spliceosome, which functions in processing of pre-mRNAs. The U1
            snRNP has been shown to be important in defining the 5' splice
            site. Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Nov 2014].
            
            Transcript Variant: This variant (1) represents the shortest
            transcript and encodes the protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146729.1, AK133115.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-184               AC151602.4         194772-194955
            185-341             AC151602.4         195649-195805
            342-404             AC151602.4         198139-198201
            405-459             AC151602.4         198331-198385
            460-524             AC151602.4         203011-203075
            525-587             AC151602.4         203159-203221
            588-669             AC151602.4         206579-206660
            670-771             AC151602.4         209646-209747
            772-859             AC151602.4         213021-213108
            860-1684            AC151602.4         213186-214010
FEATURES             Location/Qualifiers
     source          1..1684
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.28 cM"
     gene            1..1684
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /note="small nuclear ribonucleoprotein 70 (U1)"
                     /db_xref="GeneID:20637"
                     /db_xref="MGI:MGI:98341"
     exon            1..184
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     exon            185..341
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     CDS             195..1541
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /note="U1 small nuclear ribonucleoprotein 70 kDa
                     polypeptide A; U1 snRNP 70 kDa"
                     /codon_start=1
                     /product="U1 small nuclear ribonucleoprotein 70 kDa"
                     /protein_id="NP_033250.3"
                     /db_xref="CCDS:CCDS21240.1"
                     /db_xref="GeneID:20637"
                     /db_xref="MGI:MGI:98341"
                     /translation="MTQFLPPNLLALFAPRDPIPYLPPLEKLPHEKHHNQPYCGIAPY
                     IREFEDPRDAPPPTRAETREERMERKRREKIERRQQEVETELKMWDPHNDPNAQGDAF
                     KTLFVARVNYDTTESKLRREFEVYGPIKRIHMVYSKRSGKPRGYAFIEYEHERDMHSA
                     YKHADGKKIDGRRVLVDVERGRTVKGWRPRRLGGGLGGTRRGGADVNIRHSGRDDTSR
                     YDERPGPSPLPHRDRDRDRERERRERSRERDKERERRRSRSRDRRRRSRSRDKDERRR
                     SRERSKDKDRDRKRRSSRSRERARRERERKEELRGGGGGGGGGSGGGGGGDMAEPSEA
                     GDGAPDDGPPGELGPEGPDGPEEKGRDRDRERRRSHRSERERRRDRDRDRDREHKRGE
                     RGSERGRDEARGGGGSGQDNGLEGLGSDGRDMYMEAEGGDGYMAPENGYLMEAAPE"
     exon            342..404
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     exon            405..459
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     exon            460..524
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     exon            525..587
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     exon            588..669
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     exon            670..771
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     exon            772..859
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     exon            860..1684
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1656..1661
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /note="hexamer: AATAAA"
     polyA_site      1684
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /note="major polyA site"
ORIGIN      
        1 agttctggtc gcgagctgag gccgcgcggg ttgtgaggag aggtctggtt tgcgcgcgct
       61 gcgcggacca ccgaagctac ctgacgcggg cgccgccgaa gtggaagtcg gagcgggagt
      121 ttttgtttgt gaagggcggc cgcgaggcct gaggctaacg tcaggctgag gagagcgccg
      181 cgcgatttgg caagatgaca cagtttctgc ctcccaacct tctggccctt tttgcacccc
      241 gagatcccat cccatacctg ccacccctgg aaaagctgcc acatgaaaaa caccataacc
      301 aaccttactg tggcattgca ccatacatcc gagagtttga ggaccctcga gatgctcctc
      361 ctccaacaag agcagaaacc cgggaagaac gcatggagag aaagagacgg gaaaaaattg
      421 aacggcgaca gcaggaagtg gagacagagc tgaaaatgtg ggaccctcac aatgatccca
      481 atgctcaggg cgatgccttc aagactctgt tcgtggctag agtgaactat gacacaacag
      541 aatctaagct tcggagagag tttgaggtgt atggacccat caaaagaatc cacatggtat
      601 acagtaaacg ctctggaaaa ccccgtggtt atgccttcat cgagtatgag catgaacgag
      661 acatgcactc cgcttacaag cacgcagatg gcaagaagat agatggcagg agggtcctcg
      721 tggatgtgga gaggggccgg actgtgaagg gctggaggcc ccggcgccta ggaggtggcc
      781 ttggtggtac cagaagaggt ggggcagatg tgaatatcag gcattcgggc cgagatgaca
      841 cttcccgata tgatgagagg ccgggcccct ccccgcttcc acacagagac cgggaccggg
      901 accgcgagcg ggagcgcaga gaaagaagcc gagaacgaga caaggagaga gaaaggagac
      961 gctcccgctc tcgggaccgg aggaggcgct cccggagtcg agacaaggat gagcggcgac
     1021 ggtccaggga gcgtagcaag gacaaggacc gagaccgaaa gcgacgaagc agtcggagcc
     1081 gggagcgagc acgcagggaa cgggagcgaa aagaggagct tcgaggtgga ggaggtggtg
     1141 gcggtggtgg cagtggtggt ggtggcggcg gcgacatggc tgaaccctct gaagctggtg
     1201 atggagctcc tgatgatggg cctcctggag agcttggccc tgaaggccct gatggcccag
     1261 aggagaaggg ccgggatcga gaccgggaga ggcgacgtag tcaccggagt gagagggaac
     1321 gtcgtcggga ccgagaccgt gatcgggatc gagagcacaa gcgtggggag cggggcagtg
     1381 agcggggcag ggatgaggcc cggggtgggg gtggcagcgg tcaggacaat gggctggaag
     1441 ggctaggtag tgatggtcga gacatgtaca tggaggcaga gggtggtgat gggtacatgg
     1501 ccccagagaa tggatatctg atggaggcag cgcccgaatg aagaggcctg tagtggacat
     1561 gctcctaatc ttgctgtctg ccttcctagc cttttccacc ttggcttgcc tctgagggta
     1621 gatttttttt tttaatccgg tcccttggat ttaaaaataa aattaatttt ctgttgatag
     1681 tggg
//
