LOCUS       NP_033250                448 aa            linear   ROD 13-FEB-2025
DEFINITION  U1 small nuclear ribonucleoprotein 70 kDa [Mus musculus].
ACCESSION   NP_033250
VERSION     NP_033250.3
DBSOURCE    REFSEQ: accession NM_009224.6
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (residues 1 to 448)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (residues 1 to 448)
  AUTHORS   Feng,S., Li,J., Wen,H., Liu,K., Gui,Y., Wen,Y., Wang,X. and Yuan,S.
  TITLE     hnRNPH1 recruits PTBP2 and SRSF3 to modulate alternative splicing
            in germ cells
  JOURNAL   Nat Commun 13 (1), 3588 (2022)
   PUBMED   35739118
  REMARK    Publication Status: Online-Only
REFERENCE   3  (residues 1 to 448)
  AUTHORS   Nakaya,T.
  TITLE     Dissection of FUS domains involved in regulation of SnRNP70 gene
            expression
  JOURNAL   FEBS Lett 594 (21), 3518-3529 (2020)
   PUBMED   32915994
  REMARK    GeneRIF: Dissection of FUS domains involved in regulation of
            SnRNP70 gene expression.
REFERENCE   4  (residues 1 to 448)
  AUTHORS   Subramania,S., Gagne,L.M., Campagne,S., Fort,V., O'Sullivan,J.,
            Mocaer,K., Feldmuller,M., Masson,J.Y., Allain,F.H.T., Hussein,S.M.
            and Huot,M.E.
  TITLE     SAM68 interaction with U1A modulates U1 snRNP recruitment and
            regulates mTor pre-mRNA splicing
  JOURNAL   Nucleic Acids Res 47 (8), 4181-4197 (2019)
   PUBMED   30767021
  REMARK    GeneRIF: SAM68 interaction with U1A modulates U1 snRNP recruitment
            and regulates mTor pre-mRNA splicing.
REFERENCE   5  (residues 1 to 448)
  AUTHORS   Donadon,I., Pinotti,M., Rajkowska,K., Pianigiani,G., Barbon,E.,
            Morini,E., Motaln,H., Rogelj,B., Mingozzi,F., Slaugenhaupt,S.A. and
            Pagani,F.
  TITLE     Exon-specific U1 snRNAs improve ELP1 exon 20 definition and rescue
            ELP1 protein expression in a familial dysautonomia mouse model
  JOURNAL   Hum Mol Genet 27 (14), 2466-2476 (2018)
   PUBMED   29701768
  REMARK    GeneRIF: This splice-switching class of molecules is the first to
            specifically correct the ELP1 exon 20 splicing defect. Our data
            provide proof of principle of ExSpeU1s-adeno-associated virus
            particles as a novel therapeutic strategy for FD.
            Erratum:[Hum Mol Genet. 2024 Apr 18;33(9):836. doi:
            10.1093/hmg/ddae017.. PMID: 38511726]
REFERENCE   6  (residues 1 to 448)
  AUTHORS   Greidinger,E.L., Zang,Y.J., Jaimes,K., Martinez,L., Nassiri,M. and
            Hoffman,R.W.
  TITLE     CD4+ T cells target epitopes residing within the RNA-binding domain
            of the U1-70-kDa small nuclear ribonucleoprotein autoantigen and
            have restricted TCR diversity in an HLA-DR4-transgenic murine model
            of mixed connective tissue disease
  JOURNAL   J Immunol 180 (12), 8444-8454 (2008)
   PUBMED   18523312
REFERENCE   7  (residues 1 to 448)
  AUTHORS   Howell,V.M., Jones,J.M., Bergren,S.K., Li,L., Billi,A.C.,
            Avenarius,M.R. and Meisler,M.H.
  TITLE     Evidence for a direct role of the disease modifier SCNM1 in
            splicing
  JOURNAL   Hum Mol Genet 16 (20), 2506-2516 (2007)
   PUBMED   17656373
REFERENCE   8  (residues 1 to 448)
  AUTHORS   Pelsue,S. and Agris,P.F.
  TITLE     Gene usage by an anti-U1 snRNP 70K monoclonal autoantibody derived
            from a lupus-prone mouse
  JOURNAL   J Autoimmun 7 (2), 165-177 (1994)
   PUBMED   8037837
REFERENCE   9  (residues 1 to 448)
  AUTHORS   Hornig,H., Fischer,U., Costas,M., Rauh,A. and Luhrmann,R.
  TITLE     Analysis of genomic clones of the murine U1RNA-associated 70-kDa
            protein reveals a high evolutionary conservation of the protein
            between human and mouse
  JOURNAL   Eur J Biochem 182 (1), 45-50 (1989)
   PUBMED   2525092
REFERENCE   10 (residues 1 to 448)
  AUTHORS   Query,C.C. and Keene,J.D.
  TITLE     A human autoimmune protein associated with U1 RNA contains a region
            of homology that is cross-reactive with retroviral p30gag antigen
  JOURNAL   Cell 51 (2), 211-220 (1987)
   PUBMED   2959371
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC151602.4.
            
            On Jun 16, 2005 this sequence version replaced NP_033250.2.
            
            Summary: This gene encodes a subunit of the U1 snRNP (small nuclear
            ribonucleic particle), one of at least five snRNPs to comprise the
            spliceosome, which functions in processing of pre-mRNAs. The U1
            snRNP has been shown to be important in defining the 5' splice
            site. Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Nov 2014].
            
            Transcript Variant: This variant (1) represents the shortest
            transcript and encodes the protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK146729.1, AK133115.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Mus musculus"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 29.28 cM"
     Protein         1..448
                     /product="U1 small nuclear ribonucleoprotein 70 kDa"
                     /note="U1 small nuclear ribonucleoprotein 70 kDa
                     polypeptide A; U1 snRNP 70 kDa"
                     /calculated_mol_wt=51861
     Site            2
                     /site_type="acetylation"
                     /note="N-acetylthreonine.
                     /evidence=ECO:0000250|UniProtKB:P08621; propagated from
                     UniProtKB/Swiss-Prot (Q62376.2)"
     Region          3..94
                     /region_name="U1snRNP70_N"
                     /note="U1 small nuclear ribonucleoprotein of 70kDa MW N
                     terminal; pfam12220"
                     /db_xref="CDD:432406"
     Region          48..79
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62376.2)"
     Region          92..202
                     /region_name="Required for interaction with U1 RNA.
                     /evidence=ECO:0000250|UniProtKB:P08621"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62376.2)"
     Region          102..187
                     /region_name="RRM_snRNP70"
                     /note="RNA recognition motif (RRM) found in U1 small
                     nuclear ribonucleoprotein 70 kDa (U1-70K) and similar
                     proteins; cd12236"
                     /db_xref="CDD:409682"
     Site            118
                     /site_type="acetylation"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P08621; propagated from
                     UniProtKB/Swiss-Prot (Q62376.2)"
     Site            126
                     /site_type="phosphorylation"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:P08621; propagated from
                     UniProtKB/Swiss-Prot (Q62376.2)"
     Region          187..439
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q62376.2)"
     Site            226
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:21183079; propagated from
                     UniProtKB/Swiss-Prot (Q62376.2)"
     Site            268
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P08621; propagated from
                     UniProtKB/Swiss-Prot (Q62376.2)"
     Site            332
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P08621; propagated from
                     UniProtKB/Swiss-Prot (Q62376.2)"
     Site            408
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:15345747,
                     ECO:0007744|PubMed:17242355, ECO:0007744|PubMed:21183079;
                     propagated from UniProtKB/Swiss-Prot (Q62376.2)"
     Site            419
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17242355; propagated from
                     UniProtKB/Swiss-Prot (Q62376.2)"
     CDS             1..448
                     /gene="Snrnp70"
                     /gene_synonym="2700022N21Rik; 3200002N22Rik; Rnu1p70;
                     Rnulp70; Snrp70; Srnp70; U1-70; U1-70K"
                     /coded_by="NM_009224.6:195..1541"
                     /db_xref="CCDS:CCDS21240.1"
                     /db_xref="GeneID:20637"
                     /db_xref="MGI:MGI:98341"
ORIGIN      
        1 mtqflppnll alfaprdpip ylppleklph ekhhnqpycg iapyirefed prdappptra
       61 etreermerk rrekierrqq evetelkmwd phndpnaqgd afktlfvarv nydttesklr
      121 refevygpik rihmvyskrs gkprgyafie yeherdmhsa ykhadgkkid grrvlvdver
      181 grtvkgwrpr rlggglggtr rggadvnirh sgrddtsryd erpgpsplph rdrdrdrere
      241 rrersrerdk ererrrsrsr drrrrsrsrd kderrrsrer skdkdrdrkr rssrsrerar
      301 rererkeelr gggggggggs ggggggdmae pseagdgapd dgppgelgpe gpdgpeekgr
      361 drdrerrrsh rsererrrdr drdrdrehkr gergsergrd earggggsgq dngleglgsd
      421 grdmymeaeg gdgymapeng ylmeaape
//
