LOCUS       NM_001355255            2095 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus synaptosomal-associated protein 25 (Snap25),
            transcript variant 4, mRNA.
ACCESSION   NM_001355255
VERSION     NM_001355255.2
KEYWORDS    RefSeq.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Alkaslasi,M.R., Lloyd,E.Y.H., Gable,A.S., Silberberg,H.,
            Yarur,H.E., Tsai,V.S., Sohn,M., Margolin,G., Tejeda,H.A. and Le
            Pichon,C.E.
  TITLE     The transcriptional response of cortical neurons to concussion
            reveals divergent fates after injury
  JOURNAL   Nat Commun 16 (1), 1097 (2025)
   PUBMED   39870620
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  AUTHORS   Raveendran,V.A., Serranilla,M., Asgarihafshejani,A., de
            Saint-Rome,M., Cherednychenko,M., Mullany,S., Mitchell,J.A.,
            Pressey,J.C. and Woodin,M.A.
  TITLE     SNARE protein SNAP25 regulates the chloride-transporter KCC2 in
            neurons
  JOURNAL   iScience 27 (11), 111156 (2024)
   PUBMED   39507243
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2095)
  AUTHORS   Mao,Y., Meng,Y., Zou,K., Qin,N., Wang,Y., Yan,J., Chen,P.,
            Cheng,Y., Shi,W., Zhou,C., Chen,H., Sheng,J., Liu,X., Pan,J. and
            Huang,H.
  TITLE     Advanced paternal age exacerbates neuroinflammation in offspring
            via m6A modification-mediated intergenerational inheritance
  JOURNAL   J Neuroinflammation 21 (1), 249 (2024)
   PUBMED   39367406
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2095)
  AUTHORS   Zhang,L., Nagel,M., Olson,W.P., Chesler,A.T. and O'Connor,D.H.
  TITLE     Trigeminal innervation and tactile responses in mouse tongue
  JOURNAL   Cell Rep 43 (9), 114665 (2024)
   PUBMED   39215998
REFERENCE   5  (bases 1 to 2095)
  AUTHORS   Woo,M.S., Bal,L.C., Winschel,I., Manca,E., Walkenhorst,M.,
            Sevgili,B., Sonner,J.K., Di Liberto,G., Mayer,C.,
            Binkle-Ladisch,L., Rothammer,N., Unger,L., Raich,L., Hadjilaou,A.,
            Noli,B., Manai,A.L., Vieira,V., Meurs,N., Wagner,I., Pless,O.,
            Cocco,C., Stephens,S.B., Glatzel,M., Merkler,D. and Friese,M.A.
  TITLE     The NR4A2/VGF pathway fuels inflammation-induced neurodegeneration
            via promoting neuronal glycolysis
  JOURNAL   J Clin Invest 134 (16), e177692 (2024)
   PUBMED   39145444
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2095)
  AUTHORS   McMahon,H.T., Missler,M., Li,C. and Sudhof,T.C.
  TITLE     Complexins: cytosolic proteins that regulate SNAP receptor function
  JOURNAL   Cell 83 (1), 111-119 (1995)
   PUBMED   7553862
REFERENCE   7  (bases 1 to 2095)
  AUTHORS   Grimaldi,J.C., Torres,R., Kozak,C.A., Chang,R., Clark,E.A.,
            Howard,M. and Cockayne,D.A.
  TITLE     Genomic structure and chromosomal mapping of the murine CD40 gene
  JOURNAL   J Immunol 149 (12), 3921-3926 (1992)
   PUBMED   1281194
REFERENCE   8  (bases 1 to 2095)
  AUTHORS   Hess,E.J., Jinnah,H.A., Kozak,C.A. and Wilson,M.C.
  TITLE     Spontaneous locomotor hyperactivity in a mouse mutant with a
            deletion including the Snap gene on chromosome 2
  JOURNAL   J Neurosci 12 (7), 2865-2874 (1992)
   PUBMED   1613559
REFERENCE   9  (bases 1 to 2095)
  AUTHORS   Oyler,G.A., Polli,J.W., Wilson,M.C. and Billingsley,M.L.
  TITLE     Developmental expression of the 25-kDa synaptosomal-associated
            protein (SNAP-25) in rat brain
  JOURNAL   Proc Natl Acad Sci U S A 88 (12), 5247-5251 (1991)
   PUBMED   1711221
REFERENCE   10 (bases 1 to 2095)
  AUTHORS   Oyler,G.A., Higgins,G.A., Hart,R.A., Battenberg,E., Billingsley,M.,
            Bloom,F.E. and Wilson,M.C.
  TITLE     The identification of a novel synaptosomal-associated protein,
            SNAP-25, differentially expressed by neuronal subpopulations
  JOURNAL   J Cell Biol 109 (6 Pt 1), 3039-3052 (1989)
   PUBMED   2592413
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL732447.9.
            
            On Oct 12, 2022 this sequence version replaced NM_001355255.1.
            
            Transcript Variant: This variant (4) uses an alternate in-frame
            splice junction and lacks an in-frame exon but contains a different
            in-frame exon in the central coding region, compared to variant 1.
            The encoded isoform (d) is the same length but contains an
            alternate internal segment, compared to isoform a.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BU604911.1, SRR7652917.953840.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164132
                                           [ECO:0000348]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-147               AL732447.9         39077-39223
            148-281             AL732447.9         81691-81824
            282-323             AL732447.9         83934-83975
            324-372             AL732447.9         89196-89244
            373-490             AL732447.9         95680-95797
            491-604             AL732447.9         99530-99643
            605-749             AL732447.9         102959-103103
            750-2095            AL732447.9         106698-108043
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 67.56 cM"
     gene            1..2095
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /note="synaptosomal-associated protein 25"
                     /db_xref="GeneID:20614"
                     /db_xref="MGI:MGI:98331"
     exon            1..147
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /inference="alignment:Splign:2.1.0"
     exon            148..281
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /inference="alignment:Splign:2.1.0"
     CDS             210..818
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /note="isoform c is encoded by transcript variant 4; super
                     protein; synaptosomal-associated 25 kDa protein; blind
                     drunk; synaptosomal-associated protein, 25 kDa"
                     /codon_start=1
                     /product="synaptosomal-associated protein 25 isoform c"
                     /protein_id="NP_001342184.1"
                     /db_xref="GeneID:20614"
                     /db_xref="MGI:MGI:98331"
                     /translation="MAEDADMRNELEEMQRRADQLADESLESTRRMLQLVEESKDAGI
                     RTLVMLDEQGEQLERIEEGMDQINKDMKEAEKNLTDLGKFCGLCVCPCNNDAYKKAWG
                     NNQDGVVASQPARVVDEREQMAISGGFIRRVTNDARENEMDENLEQVSGIIGNLRHMA
                     LDMGNEIDTQNRQIDRIMEKADSNKTRIDEANQRATKMLGSG"
     exon            282..323
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /inference="alignment:Splign:2.1.0"
     exon            324..372
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /inference="alignment:Splign:2.1.0"
     exon            373..490
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /inference="alignment:Splign:2.1.0"
     exon            491..604
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /inference="alignment:Splign:2.1.0"
     exon            605..749
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /inference="alignment:Splign:2.1.0"
     exon            750..2095
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1933..1938
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /note="hexamer: AAGAAA"
     polyA_site      1958
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /note="major polyA site"
     regulatory      2008..2013
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /note="hexamer: ATTAAA"
     polyA_site      2029
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
     regulatory      2076..2081
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
                     /note="hexamer: ATTAAA"
     polyA_site      2095
                     /gene="Snap25"
                     /gene_synonym="Bdr; GENA70; SNAP-25; sp; SUP"
ORIGIN      
        1 atctttgatg agggcagagc tcacgttgca ttgaagaaga aacctcgggg aggtctggag
       61 ctgtctttcc ttccctccct acccggcggc tcctccactc ttgctacctg cagggatcag
      121 cggacagcat cctccggaga agacaaggtt ctttaacgaa gcaccactga cttgctggcc
      181 caagcgccca gcaacccccc accagtacca tggccgaaga cgcagacatg cgcaatgagc
      241 tggaggagat gcagaggagg gctgaccagc tggctgatga gtccctggaa agcacccgtc
      301 gcatgctgca gctggttgaa gagagtaaag atgctggcat caggactttg gttatgttgg
      361 atgagcaagg cgaacaactg gaacgcattg aggaagggat ggaccaaatc aataaggaca
      421 tgaaagaagc agaaaagaat ttgacggacc taggaaaatt ctgcgggctt tgtgtgtgtc
      481 cctgtaacaa tgatgcttac aaaaaagcct ggggcaataa tcaggatgga gtagtggcca
      541 gccagcctgc ccgtgtggtg gatgaacggg agcagatggc catcagtggt ggcttcatcc
      601 gcagggtaac aaatgatgcc cgggaaaatg agatggatga gaacctggag caggtgagcg
      661 gcatcatcgg aaacctccgc catatggctc tagacatggg caatgagatt gacacccaga
      721 atcgccagat cgacaggatc atggagaagg ctgattccaa caaaaccaga attgatgaag
      781 ccaaccaacg tgcaacaaag atgctgggaa gtggttaaat ttgcccttct gctgtgctct
      841 cctccaaatg ttgttggaca agagagctcc ttcatgcttc tctcatggta ttacctagta
      901 ggtcttgcac atacacacac acacacacac acacacacac acacacacac acacacacac
      961 acagagtcag tcacccccat tgtaaatgtc ctgtgtgctc tgtcagcttc ccaatgatac
     1021 catgtgtttt ttgtttttct ctggctctct ttctttccaa aggctgtaca tagtggtcat
     1081 ctggtggctc taattcctaa cttaagatgt cttgggtctc gttttttttt ttttcttttc
     1141 tcagtggcgt ttgctgaatg acaacaattt aggaatgctc aatgtgctgt tgattttctc
     1201 aatacacagt attgttcttg taaaactgtg acattccaca gagctactgc cacaatcctt
     1261 tcttagggtg tcaggctctg aatctctcca aatgtgctgt ctttggttcc tcatggctat
     1321 tctttgtctt tatgatttca taattagaca atgtgaaatt acataacagg cattgcacta
     1381 aaagtgatgt gatttatgca tttatgcatg agaactaaat agacttttag attcctactt
     1441 aaacaaaaac tttccatgac agtagcatac tgacaagaaa acacacaaca gcaacaataa
     1501 caaagcaaca actacgcatg ctcagtattg ggacactgtc aagattaagt cataccagca
     1561 aaacctgcag ctgtgtcacc ttctttctgt caacctacag acttactgat tataatcatc
     1621 ccattttctt tttttcacat atacatacac acacacacac acacacaaag tggatttaac
     1681 acaccattaa tcatttctag caaaatatgt gtttggctga aactatgtga aatggatgta
     1741 atatagggtt tgtcgaatgc ttttgaaagc tatgttttgg agacaatact cttgctatgt
     1801 ggaaaacgtg aagatcttct acgtctggct cttgttaatc accatctccc cgtggtttgt
     1861 catcagtaca attctctgtt gcttaatcta gagctatgca caccaaattg ctgagatgtt
     1921 tagtagctga taaagaaacc tttaaaaaat tatataaatg aatgaaatat agataaactg
     1981 tgagataaat atcattacag catgtatatt aaatccctcc tgtctcctct gttggtttgt
     2041 gaagtgattt gacattttgt agctagttta aaattattaa aaattataga tcaca
//
