LOCUS       NM_025481               5334 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus SMAD specific E3 ubiquitin protein ligase 2 (Smurf2),
            transcript variant 1, mRNA.
ACCESSION   NM_025481 XM_126673
VERSION     NM_025481.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5334)
  AUTHORS   Ahmad,S., Christova,T., Pye,M., Narimatsu,M., Song,S., Wrana,J.L.
            and Attisano,L.
  TITLE     Small Extracellular Vesicles Promote Axon Outgrowth by Engaging the
            Wnt-Planar Cell Polarity Pathway
  JOURNAL   Cells 14 (1), 56 (2025)
   PUBMED   39791757
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5334)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 5334)
  AUTHORS   Wang,L., Zha,H., Huang,J. and Shi,L.
  TITLE     Flavin containing monooxygenase 2 regulates renal tubular cell
            fibrosis and paracrine secretion via SMURF2 in AKI-CKD
            transformation
  JOURNAL   Int J Mol Med 52 (5) (2023)
   PUBMED   37800598
  REMARK    GeneRIF: Flavin containing monooxygenase 2 regulates renal tubular
            cell fibrosis and paracrine secretion via SMURF2 in AKI-CKD
            transformation.
REFERENCE   4  (bases 1 to 5334)
  AUTHORS   Stuelten,C.H., Melis,N., Subramanian,B., Tang,Y., Kimicata,M.,
            Fisher,J.P., Weigert,R. and Zhang,Y.E.
  TITLE     Smurf2 Regulates Inflammation and Collagen Processing in Cutaneous
            Wound Healing through Transforming Growth Factor-beta/Smad3
            Signaling
  JOURNAL   Am J Pathol 192 (12), 1699-1711 (2022)
   PUBMED   36063900
  REMARK    GeneRIF: Smurf2 Regulates Inflammation and Collagen Processing in
            Cutaneous Wound Healing through Transforming Growth
            Factor-beta/Smad3 Signaling.
REFERENCE   5  (bases 1 to 5334)
  AUTHORS   Kim,D., Nam,G.Y., Seo,E. and Jun,H.S.
  TITLE     Inhibition of ChREBP ubiquitination via the ROS/Akt-dependent
            downregulation of Smurf2 contributes to lysophosphatidic
            acid-induced fibrosis in renal mesangial cells
  JOURNAL   J Biomed Sci 29 (1), 31 (2022)
   PUBMED   35538534
  REMARK    GeneRIF: Inhibition of ChREBP ubiquitination via the
            ROS/Akt-dependent downregulation of Smurf2 contributes to
            lysophosphatidic acid-induced fibrosis in renal mesangial cells.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5334)
  AUTHORS   Lee,Y.S., Han,J.M., Son,S.H., Choi,J.W., Jeon,E.J., Bae,S.C.,
            Park,Y.I. and Kim,S.
  TITLE     AIMP1/p43 downregulates TGF-beta signaling via stabilization of
            smurf2
  JOURNAL   Biochem Biophys Res Commun 371 (3), 395-400 (2008)
   PUBMED   18448069
  REMARK    GeneRIF: AIMP1 negatively regulates TGF-beta signaling via
            stabilization of Smurf2.
REFERENCE   7  (bases 1 to 5334)
  AUTHORS   Wu,Q., Chen,D., Zuscik,M.J., O'Keefe,R.J. and Rosier,R.N.
  TITLE     Overexpression of Smurf2 stimulates endochondral ossification
            through upregulation of beta-catenin
  JOURNAL   J Bone Miner Res 23 (4), 552-563 (2008)
   PUBMED   18052755
REFERENCE   8  (bases 1 to 5334)
  AUTHORS   Han,G., Li,A.G., Liang,Y.Y., Owens,P., He,W., Lu,S., Yoshimatsu,Y.,
            Wang,D., Ten Dijke,P., Lin,X. and Wang,X.J.
  TITLE     Smad7-induced beta-catenin degradation alters epidermal appendage
            development
  JOURNAL   Dev Cell 11 (3), 301-312 (2006)
   PUBMED   16950122
REFERENCE   9  (bases 1 to 5334)
  AUTHORS   Kaneki,H., Guo,R., Chen,D., Yao,Z., Schwarz,E.M., Zhang,Y.E.,
            Boyce,B.F. and Xing,L.
  TITLE     Tumor necrosis factor promotes Runx2 degradation through
            up-regulation of Smurf1 and Smurf2 in osteoblasts
  JOURNAL   J Biol Chem 281 (7), 4326-4333 (2006)
   PUBMED   16373342
  REMARK    GeneRIF: Tumor necrosis factor promotes Runx2 degradation through
            up-regulation of Smurf1 and Smurf2 in osteoblasts.
REFERENCE   10 (bases 1 to 5334)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK012060.1, BU058366.1, AK013082.1, BU614468.1, AK036718.1,
            BE986450.1 and AL593847.36.
            
            On May 3, 2018 this sequence version replaced NM_025481.2.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY685230.1, SRR1660811.163042.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-535               AK012060.1         2-536
            536-1112            BU058366.1         64-640
            1113-2206           AK012060.1         1076-2169
            2207-3215           AK013082.1         254-1262
            3216-3944           BU614468.1         27-755
            3945-5103           AK036718.1         911-2069
            5104-5331           BE986450.1         16-243              c
            5332-5334           AL593847.36        19526-19528         c
FEATURES             Location/Qualifiers
     source          1..5334
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="11"
                     /map="11 70.03 cM"
     gene            1..5334
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /note="SMAD specific E3 ubiquitin protein ligase 2"
                     /db_xref="GeneID:66313"
                     /db_xref="MGI:MGI:1913563"
     exon            1..535
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             484..2730
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /EC_number="2.3.2.26"
                     /note="isoform 1 is encoded by transcript variant 1; E3
                     ubiquitin-protein ligase SMURF2; SMAD ubiquitination
                     regulatory factor 2; HECT-type E3 ubiquitin transferase
                     SMURF2"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase SMURF2 isoform 1"
                     /protein_id="NP_079757.2"
                     /db_xref="CCDS:CCDS25564.1"
                     /db_xref="GeneID:66313"
                     /db_xref="MGI:MGI:1913563"
                     /translation="MSNPGGRRNGPVKLRLTVLCAKNLVKKDFFRLPDPFAKVVVDGS
                     GQCHSTDTVKNTLDPKWNQHYDLYIGKSDSVTISVWNHKKIHKKQGAGFLGCVRLLSN
                     AINRLKDTGYQRLDLCKLGPNDNDTVRGQIVVSLQSRDRIGTGGQVVDCSRLFDNDLP
                     DGWEERRTASGRIQYLNHITRTTQWERPTRPASEYSSPGRPLSCFVDENTPITGTNGA
                     TCGHSSDPRLAERRVRSQRHRNYMSRTHLHTPPDLPEGYEQRTTQQGQVYFLHTQTGV
                     STWHDPRVPRDLSNINCEELGPLPPGWEIRNTATGRVYFVDHNNRTTQFTDPRLSANL
                     HLVLNRQNQLKDQQQQQVVPLCPDDTECLTVPRYKRDLVQKLKILRQELSQQQPQAGH
                     CRIEVSREEIFEESYRQVMKMRPKDLWKRLMIKFRGEEGLDYGGVAREWLYLLSHEML
                     NPYYGLFQYSRDDIYTLQINPDSAVNPEHLSYFHFVGRIMGMAVFHGHYIDGGFTLPF
                     YKQLLGKSITLDDMELVDPDLHNSLVWILENDITGVLDHTFCVEHNAYGEIIQHELKP
                     NGKSIPVTEENKKEYVRLYVNWRFLRGIEAQFLALQKGFNEVIPQHLLKTFDEKELEL
                     IICGLGKIDVSDWKVNTRLKHCTPDSNVVKWFWKAVEFFDEERRARLLQFVTGSSRVP
                     LQGFKALQGAAGPRLFTIHQIDACTNNLPKAHTCFNRIDIPPYESYEKLYEKLLTAIE
                     ETCGFAVE"
     exon            536..574
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            575..683
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            684..817
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            818..883
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            884..968
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            969..1052
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1255
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1256..1340
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1499
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1500..1695
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1696..1799
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1800..1914
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            1915..2093
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2094..2231
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2352
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2554
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2555..2630
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            2631..5334
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5286..5291
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /note="hexamer: AATAAA"
     regulatory      5291..5296
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /note="hexamer: AATGAA"
     polyA_site      5313
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /note="major polyA site"
     polyA_site      5334
                     /gene="Smurf2"
                     /gene_synonym="2810411E22Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggctgcggcc ccaggaccgg gttctctcag acaagagggc gggcggagga ggaagcggcc
       61 gagcacccgc ccgcctcagc agacttcgca ccgtccgctc ccaggcctcg cggcaccgag
      121 aaggggagac cgaggcggac cgcgaggaca agaggcggcg ggggcgcggg gcggaggcgg
      181 cgcggccggc cgcggctgcc gctttgttgt gtggcccggg ccgaggaagc agaagtggga
      241 ggagggggga gctctgcgtc ccgctccctc cgcggctcat ggcgacggcg ctcggcacat
      301 ccgggaccct ccggccctgg cggccgcggc gcgggctgtt cggaccccgg ccccggccgc
      361 tgtggtgact cctctgcgcc tcgccgtcgg cccagcccag cccagtccgc ccgcccgccc
      421 gccggcccgc cggccgcgag cgccgccgcc gccgccgccg ccacctcccc cgccgctggg
      481 gacatgtcta accccggagg ccggaggaac gggcccgtca agctgcgcct gacagtactc
      541 tgtgcaaaaa acttggtgaa aaaggatttt ttccgtcttc ctgatccatt tgctaaggtg
      601 gtagttgatg gttctgggca gtgccattct acagatactg tgaagaacac acttgatcca
      661 aagtggaatc agcattatga cctgtatatc ggaaagtctg attcagttac gatcagcgta
      721 tggaatcata agaagatcca taagaagcaa ggtgctggat ttcttggttg tgttcgtctt
      781 ctttccaatg ccatcaaccg cctcaaggac actggttatc agaggttgga tttatgcaaa
      841 ctggggccaa atgacaatga tacagttcga ggacaaattg tagtaagtct tcagtccaga
      901 gaccgaatag gcacgggagg acaagttgtg gactgtagtc gtttgtttga caatgatttg
      961 ccagatggtt gggaagaaag gcggactgcc tcgggaagaa tccagtatct aaaccacatc
     1021 acaagaacta cacagtggga acgcccaaca cgaccggcgt cagaatattc tagtcctggc
     1081 agacccctca gctgctttgt ggatgagaat actccaatta ctggaacaaa tggtgcaacg
     1141 tgtggacatt cttcagaccc cagactagca gagagaagag tcaggtccca gcgacataga
     1201 aattacatga gcaggacaca cttacacact cctccagacc taccggaagg ctatgaacaa
     1261 aggacaacgc agcagggtca ggtatatttc ttacatactc agactggtgt gagcacatgg
     1321 catgatccac gagtgcctag ggatcttagc aacatcaatt gtgaagagct cggtcctttg
     1381 cctcctggat gggagatccg caataccgca acaggaagag tttatttcgt tgaccataac
     1441 aacagaacaa cacaatttac agatccccgg ctctctgcta acttgcattt agttttaaat
     1501 cgtcagaacc agttgaaaga ccaacagcaa cagcaagtgg tgccattgtg tcccgatgac
     1561 actgagtgtc tgacagtgcc aagatacaag cgagatttgg ttcaaaaact aaaaatcttg
     1621 cggcaagaac tttcccaaca gcagcctcaa gctggccact gccgtattga ggtgtctagg
     1681 gaagagattt ttgaggaatc atatagacag gtcatgaaaa tgaggccaaa agatctatgg
     1741 aagcgattaa tgataaaatt tcgtggagaa gaaggtcttg actatggagg agttgccagg
     1801 gaatggttgt atctcctgtc acatgaaatg ttgaatcctt actatggtct cttccagtat
     1861 tctagagatg atatctacac attgcagatc aatcctgatt ctgcagttaa tccggaacat
     1921 ttatcctatt tccactttgt tggacggata atgggaatgg ctgtattcca tggacattat
     1981 attgacggtg gcttcacatt acctttttat aaacagttgc ttgggaagtc aattactttg
     2041 gatgacatgg agctagttga cccagacctt cataatagtt tagtgtggat acttgaaaac
     2101 gatatcacag gtgttctgga ccataccttc tgtgttgagc ataatgcata tggtgaaata
     2161 atccagcatg aactcaaacc aaatggcaaa agtatccctg ttactgaaga aaataaaaaa
     2221 gaatatgtca ggctttatgt caactggaga tttctccgag gcatcgaagc tcagttcctg
     2281 gcgctgcaga agggctttaa tgaagtcatt ccccagcacc tgctgaagac atttgatgag
     2341 aaggagctgg agctcattat ttgtggactt ggcaaaattg atgtgagtga ctggaaggtc
     2401 aacacccggt taaaacactg taccccagac agcaatgtcg tcaagtggtt ctggaaagcg
     2461 gtggagtttt ttgatgaaga gagacgagca cggttgctcc agtttgtgac agggtcctct
     2521 cgagtgcctc tgcagggctt caaagcacta caaggtgctg caggcccacg gctctttacc
     2581 atacaccaga ttgatgcctg cacgaacaac ttgccaaaag cccacacttg cttcaatcga
     2641 atagacattc caccctatga aagctatgag aagctctatg aaaagctgct aacggccatc
     2701 gaggagacat gtggctttgc tgtggaatga tgggcaccaa ggacttacct acagctctta
     2761 tttattccct ggccaacagc ctccttcagc agcgcttcag agaacgctgg gatacaggac
     2821 agcctcctcc cctgttcctt agattctagg acactgggag gggaaaggac aattttgaaa
     2881 ttccttttca aggaaaagta aaggtcttta tgctttgcca tgaggacaca ctcagctgct
     2941 atttaaaaat taataccttg aacctaaaga atgctgaccc cctgcgtctc cagagttagg
     3001 cagtattcta cacttaaaag actactacta tttttataca aggttatctc cccaaattgc
     3061 ttgtcagact catgtctctc ccgcatagca agacaactcc agcagtcggt acaagtcaca
     3121 cttcattttg attgagtaca tgatttttga acaactccta tacctgctct ttgtaagaaa
     3181 ttaaataaag ttattttgga ttgttccacc tttgtaaaca attggctaaa gagggatcat
     3241 taccttgatg aaattaagcc atttacatgt ttagttgtgt ttttctatag cagagtgtta
     3301 tattttgcat tatgtgggtc accgtagctt aagtggggtt ttatattttt caagtgtgac
     3361 tttcttggaa attcttaatg cagccattta aacagtttat ttgctaccct tcctgttttt
     3421 aagatgactt aaaaaaaaaa aaaagacctc atgttcttga atcaagaaga tcaggtattt
     3481 tggatttact ttcatcttaa attgaaatac tgcattttta tagcttctca gtatgtgatg
     3541 gggtggattt tcattctttg ctgggtcagc ttgtctttta tatgctattt ctataaacca
     3601 aagtctctaa taagtacatc taacttatat tttaattaag tttctaaaag gaaaattttt
     3661 gctctaaaaa tcgtttaaca agtttgagag ccctgctttt acctcattgg ttgagctgga
     3721 ggtggtagtg ggcatggtgg gccaagaatg tcatacatgc atgacgtcag ctgggctgac
     3781 catgcccgtg atcctggctc ctgggaagct aaggcagaag ggccagttca agtttgaggc
     3841 cagcctgagc gacacagcag accttgcctc acataagagt gactctaagg cagtcgtata
     3901 atttactcta gaactaaaga gttcatgaag tgagggactg gaggccaggc tgccacactg
     3961 ctgacttcaa gtgtggaggt tacgctttca aggtgtgtgg cccatcctgt gatctagaag
     4021 gtaacaaaaa ttctggggtg aggggttgca accatttaaa aaaaaatcac attggatttt
     4081 aaaattcaac catcagaaca atgcactatt atttttaaac atggatgtga aagaagaata
     4141 gcagattttg ctgagcttgg cacagggcca tgaggagcac ttgacaggaa gtgtaactca
     4201 gcctggtggc cgacacccgt gatcctgtca ctggggaggc agaggcagaa gcgtcagcat
     4261 gccaaggcca gtctcaccta cacagtggat tgaggcttcc ttgagaccct gtctcagagg
     4321 acagggatgc agtgctaagg agacgctgct ttcagtggtt ttccatgcag tgtaacacag
     4381 gcaaatccca ctgctttctg agtgcctttt aactttatta cagtgacttg acagagcttt
     4441 agaggaaact gccctatgta acggtcttca atagcacaga catcaaaaac atggtttaga
     4501 tttcgatttc aaaattacat tacctgatta cttccaaata ggtgctaagc aaacaaaaat
     4561 ccccagtgtc acagaacctg gacttgcctt gtgtttaatg tgaatattta tctgtcccca
     4621 cgtcgcctgc ctccaggcgc tctgttccat caggaaagct tcattctgac caatggttcc
     4681 ctgtgctcac atgcagtaag aggccactat tttctggcac aaagttttaa agcagtgctg
     4741 tgtatcctgt acatttgttt tgcagtttgt tgctctgtat tttacagtgc tctccccacc
     4801 ccactcacat ttacaggcca gagcacaaat ttctcttctc ctaaatccac tataatttgc
     4861 tttcctagac aacactaaat ctgccctgag cttgcagtgc atcggacaga tggcactggt
     4921 gggtgttagc cgcttccagt gcctgcaggg tcagcagcac agcctcgccc ttcagctgca
     4981 gcttacggtc ggggccacgt gtggtggtta atgttctcac actaggatga tgattgtacc
     5041 aaatgctgag ttgctgattc tagatgtaat tagttcactt tatgtaatat tctccttttc
     5101 tctaaactga ctttaaaagc aaagttttat catttccatt gtatttatta cagaatgttt
     5161 tagccttgat attctttgta atttcactca tttgttacat gagctttatc aataacatct
     5221 gtataaatgg tttttaaaaa ttaactatgt acgtatgtat gtatgtgccc atgccgaagc
     5281 tgagcaataa aatgaatgct gtttgcactg tcacagcagc gaggtttgta agaa
//
