LOCUS       NM_001038627            5333 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus SMAD specific E3 ubiquitin protein ligase 1 (Smurf1),
            transcript variant 1, mRNA.
ACCESSION   NM_001038627
VERSION     NM_001038627.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5333)
  AUTHORS   Ahmad,S., Christova,T., Pye,M., Narimatsu,M., Song,S., Wrana,J.L.
            and Attisano,L.
  TITLE     Small Extracellular Vesicles Promote Axon Outgrowth by Engaging the
            Wnt-Planar Cell Polarity Pathway
  JOURNAL   Cells 14 (1), 56 (2025)
   PUBMED   39791757
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5333)
  AUTHORS   Hung,Y.S., Lin,W.M., Wang,Y.C., Kuo,W.C., Chen,Y.Y., Fann,M.J.,
            Yu,J.Y. and Wong,Y.H.
  TITLE     Protogenin facilitates trunk-to-tail HOX code transition via
            modulating GDF11/SMAD2 signaling in mammalian embryos
  JOURNAL   Commun Biol 7 (1), 1669 (2024)
   PUBMED   39702818
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5333)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 5333)
  AUTHORS   Jiang,F., Yang,Y., Ni,Y., Qin,Y., Yuan,F., Ju,R. and Wu,M.
  TITLE     Smurf1 Modulates Smad Signaling Pathway in Fibrotic Cataract
            Formation
  JOURNAL   Invest Ophthalmol Vis Sci 65 (2), 18 (2024)
   PUBMED   38324299
  REMARK    GeneRIF: Smurf1 Modulates Smad Signaling Pathway in Fibrotic
            Cataract Formation.
REFERENCE   5  (bases 1 to 5333)
  AUTHORS   Li,X., Tao,S., Xu,Z., Ren,Y., Xiang,W. and He,X.
  TITLE     SMURF1 activates the cGAS/STING/IFN-1 signal axis by mediating YY1
            ubiquitination to accelerate the progression of lupus nephritis
  JOURNAL   Autoimmunity 56 (1), 2281235 (2023)
   PUBMED   37994046
  REMARK    GeneRIF: SMURF1 activates the cGAS/STING/IFN-1 signal axis by
            mediating YY1 ubiquitination to accelerate the progression of lupus
            nephritis.
REFERENCE   6  (bases 1 to 5333)
  AUTHORS   Zhao,M., Qiao,M., Oyajobi,B.O., Mundy,G.R. and Chen,D.
  TITLE     E3 ubiquitin ligase Smurf1 mediates core-binding factor
            alpha1/Runx2 degradation and plays a specific role in osteoblast
            differentiation
  JOURNAL   J Biol Chem 278 (30), 27939-27944 (2003)
   PUBMED   12738770
  REMARK    GeneRIF: Smurf1 appears to be an important regulatory factor in
            osteoblast differentiation and a potential molecular target for
            identification of bone anabolic agents
REFERENCE   7  (bases 1 to 5333)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Aizawa,H., Yuasa,S.,
            Nakajima,D., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: II. The complete nucleotide sequences of 400 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (1), 35-48 (2003)
   PUBMED   12693553
REFERENCE   8  (bases 1 to 5333)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
REFERENCE   9  (bases 1 to 5333)
  AUTHORS   Jiao,K., Zhou,Y. and Hogan,B.L.
  TITLE     Identification of mZnf8, a mouse Kruppel-like transcriptional
            repressor, as a novel nuclear interaction partner of Smad1
  JOURNAL   Mol Cell Biol 22 (21), 7633-7644 (2002)
   PUBMED   12370310
REFERENCE   10 (bases 1 to 5333)
  AUTHORS   Zhu,H., Kavsak,P., Abdollah,S., Wrana,J.L. and Thomsen,G.H.
  TITLE     A SMAD ubiquitin ligase targets the BMP pathway and affects
            embryonic pattern formation
  JOURNAL   Nature 400 (6745), 687-693 (1999)
   PUBMED   10458166
  REMARK    GeneRIF: Initial identification of Smurf1 as an ubiquitin ligase
            that inhibits BMP signaling by targeting Smad1/5 for proteasomal
            destruction in cultured cells and Xenopus embryos.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC113295.5, CX568994.1, CB524571.1, AK015264.1 and AK034736.1.
            
            Transcript Variant: This variant (1) encodes the longer protein
            (isoform 1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data because no single C57BL/6J strain transcript
            was available for the full length of the gene. The extent of the
            sequence in this record is supported by orthologous human data.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK154491.1, SRR14777535.1927659.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-330               AC113295.5         84682-85011
            331-369             AC113295.5         147934-147972
            370-478             AC113295.5         149227-149335
            479-1081            CX568994.1         79-681
            1082-1646           CB524571.1         160-724
            1647-2286           AK015264.1         1046-1685
            2287-2589           AK034736.1         885-1187
            2590-5333           AC113295.5         171274-174017
FEATURES             Location/Qualifiers
     source          1..5333
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 84.83 cM"
     gene            1..5333
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /note="SMAD specific E3 ubiquitin protein ligase 1"
                     /db_xref="GeneID:75788"
                     /db_xref="MGI:MGI:1923038"
     exon            1..330
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     CDS             276..2471
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /EC_number="2.3.2.26"
                     /note="isoform 1 is encoded by transcript variant 1; Smad
                     ubiquitination regulatory factor 1; E3 ubiquitin-protein
                     ligase SMURF1; HECT-type E3 ubiquitin transferase SMURF1"
                     /codon_start=1
                     /product="E3 ubiquitin-protein ligase SMURF1 isoform 1"
                     /protein_id="NP_001033716.1"
                     /db_xref="CCDS:CCDS39380.1"
                     /db_xref="GeneID:75788"
                     /db_xref="MGI:MGI:1923038"
                     /translation="MSNPGTRRNGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKIVVDG
                     SGQCHSTDTVKNTLDPKWNQHYDLYVGKTDSITISVWNHKKIHKKQGAGFLGCVRLLS
                     NAISRLKDTGYQRLDLCKLNPSDTDAVRGQIVVSLQTRDRIGGGGSVVDCRGLLENEG
                     TVYEDSGPGRPLSCLMEEPAPYTDGTGAAAGGGNCRFVESPSQDQRLLVQRLRNPEVR
                     GPLQTPQNRPHGHQSPELPEGYEQRTTVQGQVYFLHTQTGVSTWHDPRIPRDLNSVNC
                     DELGPLPPGWEVRSTVSGRIYFVDHNNRTTQFTDPRLHHIMNHQCQLKEPSQPLQLPS
                     EGSVEDEELPAQRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMK
                     MRPKDLKKRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQIN
                     PDSSINPDHLSYFHFVGRIMGLAVFHGHYINGGFTVPFYKQLLGKPIQLSDLESVDPE
                     LHKSLVWILENDITPVLDHTFCVEHNAFGRILQHELKPNGRNVPVTEENKKEYVRLYV
                     NWRFMRGIEAQFLALQKGFNELIPQHLLKPFDQKELELIIGGLDKIDLNDWKSNTRLK
                     HCVADSNIVRWFWQAVETFDEERRARLLQFVTGSTRVPLQGFKALQGSTGAAGPRLFT
                     IHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLTAVEETCGFAVE"
     exon            331..369
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            370..478
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            479..612
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            613..678
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            679..754
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            755..996
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            997..1081
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            1082..1228
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1427
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1531
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            1532..1646
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            1647..1825
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1963
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            1964..2084
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            2085..2286
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            2287..2371
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     exon            2372..5333
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2697..2702
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /note="hexamer: AATAAA"
     polyA_site      2724
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
     regulatory      5309..5314
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /note="hexamer: AATAAA"
     polyA_site      5331
                     /gene="Smurf1"
                     /gene_synonym="4930431E10Rik; mKIAA1625"
                     /note="major polyA site"
ORIGIN      
        1 ggaagcagcg agggcggcgg gcgtccggct gcgagcgcgg cggctccggg ctgggccagc
       61 caggggcggc tcctggcggc ggcgcggccg agcgctcggt tctacggttc cagcccgtgg
      121 tctccggccc aacgcctgcg ccgcgcactc ggcgtcccgg ctcggacccc ggcgcccagc
      181 ccgtggccgt aaccttgagg cggcggcggg gccgggccgg gccgggctgg ggggcggcgg
      241 cactggatcc gaggccggcc ggccgctggc gggagatgtc gaaccccggg acccgtagga
      301 acggctccag catcaagatc cgtctgacag tattgtgtgc caagaacctt gcaaagaaag
      361 acttcttcag actccccgac ccctttgcca agattgttgt ggacggctct gggcagtgcc
      421 actcaaccga cactgtgaaa aacaccctgg acccaaagtg gaaccagcac tatgacctgt
      481 atgttgggaa aacggactcg ataaccatca gtgtgtggaa ccacaagaag atccacaaga
      541 agcagggggc tggcttcctg ggctgtgtgc ggctgctctc caatgccatc agcagattga
      601 aagacactgg ctaccagcgt ttggatctat gcaaactaaa tccctcagat actgatgcag
      661 ttcgtggcca aatagtggtc agtttacaga cccgagacag aataggcggt ggagggtcag
      721 tggtggactg cagagggctg ctggagaacg aaggaacagt gtatgaagac tcaggccctg
      781 gaaggccgct cagctgcctc atggaggaac ctgccccata tacagatggt actggtgcag
      841 cagcaggagg cgggaactgc aggtttgtgg agtctccaag ccaagatcag agactcctgg
      901 tacagcgact ccgaaatcct gaggttcgag ggcccttaca gacaccccag aaccgaccac
      961 atggccacca gtcgccagag ctgcctgaag gctatgagca aaggacaaca gtgcagggac
     1021 aagtttactt tttgcacacg cagactggag tcagtacatg gcatgacccc aggatcccca
     1081 gagaccttaa cagtgtgaac tgcgatgaac ttgggccact gcctccaggc tgggaagtcc
     1141 gaagcacagt gtcgggaaga atctattttg tagatcacaa taataggaca acccagttta
     1201 cagatccacg gcttcaccac atcatgaatc accagtgcca actcaaggag cccagccagc
     1261 cgctgcagct gcccagtgag ggctccgtgg aggacgagga gcttcctgcc cagagatatg
     1321 agagggactt agtccagaag ctcaaagttc tcaggcacga gctctctctt cagcagcccc
     1381 aggctggtca ctgtcgaata gaagtctcca gagaagagat atttgaggag tcgtatcgcc
     1441 agatcatgaa gatgcggcca aaagacctaa agaagcgcct gatggtgaag ttccgagggg
     1501 aggaaggttt ggactatggt ggagtggctc gggagtggct gtatttgttg tgccatgaaa
     1561 tgttgaaccc gtactatgga ctcttccagt attccacgga caatatttac acactgcaga
     1621 tcaacccaga ttcttctatc aaccctgacc atctgtcata cttccacttt gtgggtcgca
     1681 tcatgggtct ggctgtgttc cacggacact acataaatgg gggtttcaca gttccgttct
     1741 acaagcagct cctggggaag ccaatccagc tgtcggacct ggagtccgtg gacccagaac
     1801 tgcataagag cttggtgtgg attctagaga atgacatcac gcctgtgttg gatcatacct
     1861 tctgcgtgga gcacaacgct ttcgggcgga ttctccagca tgaactgaaa cccaatggca
     1921 gaaatgtgcc tgtcactgag gagaacaaga aggaatacgt ccggctgtat gtgaactgga
     1981 ggtttatgag aggaatcgaa gcccagttct tagcacttca gaaggggttt aacgaactca
     2041 ttccccaaca cttgctgaag ccctttgacc agaaggaact agagctgata ataggtgggc
     2101 tggataagat agacctgaac gactggaagt ccaacacccg gctgaaacac tgtgtggcag
     2161 acagcaacat cgtcaggtgg ttctggcagg cggtggagac cttcgatgag gagaggagag
     2221 ccagactcct gcagtttgtg acaggatcca caagagttcc tctccaaggc ttcaaggctc
     2281 tgcaaggctc tacaggcgcg gcagggcccc ggctgttcac cattcacctg atagacgcca
     2341 atacagacaa cctgcccaag gcccatacct gctttaatcg gatcgacatc ccaccctatg
     2401 agtcctatga gaagctctat gagaagctgc tgacagcggt ggaggaaacc tgtggctttg
     2461 cagtggagtg aagagcagcc agtggccaca gagtcttgct cacaaccacc agacccaaag
     2521 catatggcgt ctgcacgcct cccgcgtggc gggctgaggc ctgagattcc agaaaccgag
     2581 ggaaaaggct cgtctccctc ctcctttgga gagggcaggc caggggactt tcctaggtgg
     2641 ctcccaccca tttattctcc tttattatag tttgcccacc cctccatcac ccatccaata
     2701 aaacgcagcc aggtttcgcc ctcagcctcc ttggcacggt gggaaggctg tggtgtctcc
     2761 gcgagcatcg acccatggtc aggagccgct gggccgctga gggttgcgtg gtctctgtgc
     2821 tagctcccct tgactggagc ctcaaagccc cctttcaagt tcagcagctg ccgacagctt
     2881 actgagctca agttccagag gcgtccatca caggctcaca gcctgttcag gtagcaggtg
     2941 gatttctaag cagcagaagg taaatgcaca gtgtatgctt gctggaactc caccgtggca
     3001 ggacactcga ggcaggatgt gttttcccct ttgataggta agggaattgc agcggccgtc
     3061 actggaagcc caggtacttc cttttcgcag tatcctggct aaaaagaatg gttttgcaca
     3121 ggggaaaaaa aaaatcttta aaagtacaat ttgttgtgtt ctctacccag tttgaaatta
     3181 tcacgtcatg gagctaagca aagaataacc aaaaccagcc tcagaccccc gggtccagct
     3241 ctgctcctgt gcctggggat ccggaggctc ctggctgtct tgaaatggac ttttatatgg
     3301 ggattgctaa gagctaaact aacagaagag ccttgcacca cagccactgg gcgggacagc
     3361 aggagaggcc gctgacctgg gcccctgatg cagctcccca gggtcggggc tgtgcacaaa
     3421 gcaggtgtga tggagcgtgt ggcagcaggg tggggtgggg ctttacctcc tacgtggccc
     3481 tcgtccccag tggcaacgtg atgctaacag ttctcctcag cttgcttgct tccttgtctt
     3541 ctgaggagca aggccaaagc cagcatctct aacacatctc taaccccacg tccaggttgc
     3601 acaggcttca cagcgctgac tctcacagca gagcggcagc catgcctccg tttgtggttt
     3661 tgttgttttt gtttgtggtg ggggcgagaa cagggtaatt cattagcaag acatttcatt
     3721 ggtaccgggt tcaggttgtc tggggaataa tgggaggtcc tgcgggccac cagcttttgt
     3781 gttctttctg ttgcttaccc tcaggctggg gatagggaag tgtcttccct tctgttcctg
     3841 ctgcttctgt ccagtgtggt ctggaagcag ggctgaggtg atggggagtg gattcatagg
     3901 tcacatacgc ccccaaccca agaggtttgt ccagcatagg agtcagctgc gctctgggtt
     3961 gccaggtctg gggacagaaa ggatgccagg tagacctcac cgcacaagga ccgaacccgc
     4021 cccacctccc aggccagact ggggccttac tagtctctca attctgcctt gccccagtag
     4081 ggcttctgta cttcatctac cttcatgctt ccgagggtgt tctaaaaccc agctccctcg
     4141 tgactctcgg attcaaaagg cgccaggaag cctgtgccct aagtctcagc cagtccacag
     4201 gaagcatcca gaccccgcag agtgacacgc ttccggagac tggacacact gcctgttgcc
     4261 tttgcacact ggcccagtct gcagggtcct gtgctctgag gatgcactgc aggaatgtca
     4321 agtcacactg tttgtgccac ggaagtgatt gggagacaag ccttaggagt cctgtgtcag
     4381 atgccctctg aggaagatcc agagcttagg ccctcagggg ctgactgatt ggcgcacata
     4441 ccacccactg aggcttctac cagccgttgt gcctgcgtcg cctgtgggtc ccctcattgc
     4501 cagttctgag tgtcacaacc tcccaggtct gttcatgtgc agcactgtcc tctgttggta
     4561 gcgctgcact tccttgtctg tctaccatgg ttaggccctt ctgacgcccc tcaaagaaca
     4621 ctgcgtggcc agcccacggc acagctcctg gttgccctgc ttgggtctgg gtgaagccct
     4681 ttcacgtaag atctcgatcg tcagccctcc tggtggcccc acatcaccca gatgcttcag
     4741 gagcctggct ccgcaggctc tcctcaccag cggccggcac ccacactcag ggacgcagca
     4801 gacaccactc tactccgcag tgcttcagag aggacgcgcc aggctgcagc agtgctgtgg
     4861 cagcaggtcg cagtctcatg cttggttagg aggatctgcc acaaggtgga ttactgaccc
     4921 tgcactggct tggccacatg gccaggttca ggtcaggtgg gcgctggact gaccttctgc
     4981 agagaacacg ccccacccta ctgcagcctg tgggccacat ggcagccttc tgacggtgtg
     5041 gatttttaaa ctgtgtttat aatgtattct aagttatttt gtaaggtcct gtttgaagtg
     5101 ctgctgctag ccctagttgc tctcccagtt tttattgtcc attttcctgt gacagttgta
     5161 cactaatgtt ctgttttatg tcagaatcga aagtatttaa agaaacgcta gttctattta
     5221 atgcagttgt ggaaccagct ggaaacacga agcgttgact gtagagcagg ctggacccgg
     5281 aaggtcaggt tcattttgtt acatatgcaa taaactcaca actttacatt ttc
//
