LOCUS       NM_027885               3611 bp    mRNA    linear   ROD 02-JUN-2024
DEFINITION  Mus musculus single-strand selective monofunctional uracil DNA
            glycosylase (Smug1), mRNA.
ACCESSION   NM_027885 XM_001004064
VERSION     NM_027885.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Carracedo,S., Lirussi,L., Alsoe,L., Segers,F., Wang,C.,
            Bartosova,Z., Bohov,P., Tekin,N.B., Kong,X.Y., Esbensen,Q.Y.,
            Chen,L., Wennerstrom,A., Kroustallaki,P., Ceolotto,D., Tonjes,A.,
            Berge,R.K., Bruheim,P., Wong,G., Bottcher,Y., Halvorsen,B. and
            Nilsen,H.
  TITLE     SMUG1 regulates fat homeostasis leading to a fatty liver phenotype
            in mice
  JOURNAL   DNA Repair (Amst) 120, 103410 (2022)
   PUBMED   36244177
  REMARK    GeneRIF: SMUG1 regulates fat homeostasis leading to a fatty liver
            phenotype in mice.
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Kroustallaki,P., Lirussi,L., Carracedo,S., You,P., Esbensen,Q.Y.,
            Gotz,A., Jobert,L., Alsoe,L., Saetrom,P., Gagos,S. and Nilsen,H.
  TITLE     SMUG1 Promotes Telomere Maintenance through Telomerase RNA
            Processing
  JOURNAL   Cell Rep 28 (7), 1690-1702 (2019)
   PUBMED   31412240
REFERENCE   3  (bases 1 to 3611)
  AUTHORS   Alsoe,L., Sarno,A., Carracedo,S., Domanska,D., Dingler,F.,
            Lirussi,L., SenGupta,T., Tekin,N.B., Jobert,L., Alexandrov,L.B.,
            Galashevskaya,A., Rada,C., Sandve,G.K., Rognes,T., Krokan,H.E. and
            Nilsen,H.
  TITLE     Uracil Accumulation and Mutagenesis Dominated by Cytosine
            Deamination in CpG Dinucleotides in Mice Lacking UNG and SMUG1
  JOURNAL   Sci Rep 7 (1), 7199 (2017)
   PUBMED   28775312
  REMARK    GeneRIF: we showed that SMUG1 efficiently prevent genomic uracil
            accumulation, even in the presence of UNG, and identified
            mutational signatures associated with combined UNG and SMUG1
            deficiency.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3611)
  AUTHORS   Lewandowski,J.P., Du,F., Zhang,S., Powell,M.B., Falkenstein,K.N.,
            Ji,H. and Vokes,S.A.
  TITLE     Spatiotemporal regulation of GLI target genes in the mammalian limb
            bud
  JOURNAL   Dev Biol 406 (1), 92-103 (2015)
   PUBMED   26238476
REFERENCE   5  (bases 1 to 3611)
  AUTHORS   Dingler,F.A., Kemmerich,K., Neuberger,M.S. and Rada,C.
  TITLE     Uracil excision by endogenous SMUG1 glycosylase promotes efficient
            Ig class switching and impacts on A:T substitutions during somatic
            mutation
  JOURNAL   Eur J Immunol 44 (7), 1925-1935 (2014)
   PUBMED   24771041
  REMARK    GeneRIF: Nonetheless, using a highly MSH2-dependent mechanism,
            Ung(-/-) Smug1(-/-) mice can still produce detectable levels of
            switched isotypes, especially IgG1
REFERENCE   6  (bases 1 to 3611)
  AUTHORS   An,Q., Robins,P., Lindahl,T. and Barnes,D.E.
  TITLE     C --> T mutagenesis and gamma-radiation sensitivity due to
            deficiency in the Smug1 and Ung DNA glycosylases
  JOURNAL   EMBO J 24 (12), 2205-2213 (2005)
   PUBMED   15902269
REFERENCE   7  (bases 1 to 3611)
  AUTHORS   Blackshaw,S., Harpavat,S., Trimarchi,J., Cai,L., Huang,H.,
            Kuo,W.P., Weber,G., Lee,K., Fraioli,R.E., Cho,S.H., Yung,R.,
            Asch,E., Ohno-Machado,L., Wong,W.H. and Cepko,C.L.
  TITLE     Genomic analysis of mouse retinal development
  JOURNAL   PLoS Biol 2 (9), E247 (2004)
   PUBMED   15226823
REFERENCE   8  (bases 1 to 3611)
  AUTHORS   Wibley,J.E., Waters,T.R., Haushalter,K., Verdine,G.L. and
            Pearl,L.H.
  TITLE     Structure and specificity of the vertebrate anti-mutator uracil-DNA
            glycosylase SMUG1
  JOURNAL   Mol Cell 11 (6), 1647-1659 (2003)
   PUBMED   12820976
  REMARK    GeneRIF: The structure and specificity of SMUG1 have been solved.
REFERENCE   9  (bases 1 to 3611)
  AUTHORS   Masaoka,A., Matsubara,M., Hasegawa,R., Tanaka,T., Kurisu,S.,
            Terato,H., Ohyama,Y., Karino,N., Matsuda,A. and Ide,H.
  TITLE     Mammalian 5-formyluracil-DNA glycosylase. 2. Role of SMUG1
            uracil-DNA glycosylase in repair of 5-formyluracil and other
            oxidized and deaminated base lesions
  JOURNAL   Biochemistry 42 (17), 5003-5012 (2003)
   PUBMED   12718543
REFERENCE   10 (bases 1 to 3611)
  AUTHORS   Nilsen,H., Haushalter,K.A., Robins,P., Barnes,D.E., Verdine,G.L.
            and Lindahl,T.
  TITLE     Excision of deaminated cytosine from the vertebrate genome: role of
            the SMUG1 uracil-DNA glycosylase
  JOURNAL   EMBO J 20 (15), 4278-4286 (2001)
   PUBMED   11483530
COMMENT     PROVISIONAL REFSEQ: This record has not yet been subject to final
            NCBI review. The reference sequence was derived from AC162693.5.
            
            On Mar 21, 2007 this sequence version replaced NM_027885.2.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC062960.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164133 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-13                AC162693.5         30533-30545
            14-185              AC162693.5         31843-32014
            186-326             AC162693.5         32592-32732
            327-397             AC162693.5         35590-35660
            398-699             AC162693.5         35899-36200
            700-3611            AC162693.5         37616-40527
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 58.2 cM"
     gene            1..3611
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /note="single-strand selective monofunctional uracil DNA
                     glycosylase"
                     /db_xref="GeneID:71726"
                     /db_xref="MGI:MGI:1918976"
     exon            1..13
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            14..185
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            186..326
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            327..397
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            398..699
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /inference="alignment:Splign:2.1.0"
     CDS             409..1248
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /codon_start=1
                     /product="single-strand selective monofunctional uracil
                     DNA glycosylase"
                     /protein_id="NP_082161.2"
                     /db_xref="CCDS:CCDS27898.1"
                     /db_xref="GeneID:71726"
                     /db_xref="MGI:MGI:1918976"
                     /translation="MAASQTFPLGPTHEPASALMEPLPCTRSLAEGFLEEELRLNAEL
                     SQLQFPEPVGVIYNPVDYAWEPHRNYVTRYCQGPKEVLFLGMNPGPFGMAQTGVPFGE
                     VNVVRDWLGVGGPVLTPPQEHPKRPVLGLECPQSEVSGARFWGFFRTLCGQPQVFFRH
                     CFVHNLCPLLFLAPSGRNLTPAELPAKQREQLLSICDAALCRQVQLLGVRLVVGVGRL
                     AEQRARRALAGLTPEVQVEGLLHPSPRSAQANKGWEAAARERLQELGLLPLLTDEGSA
                     RPT"
     exon            700..3611
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3574..3579
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /note="hexamer: AATAAA"
     polyA_site      3611
                     /gene="Smug1"
                     /gene_synonym="1200013B09Rik; A930006H09Rik"
                     /note="major polyA site"
ORIGIN      
        1 ggagcttgtg cagggacttg caaagcaaag gtcccagaaa acccagttcg ccacaccgtc
       61 ctcagctttc cagaatgttc tgggagcctg tgtggacaaa ccagatttct agcctgctat
      121 ggtgacacat ccaggtaatc ccagcactcg ggagactgag gcaggaagaa ggccactggt
      181 tggagccaaa atgccaggca gttcattgtt ctgagaaaaa cagcatgaga gaggaaagct
      241 agaacctagg tcacagattc aggggttcca gtccctggct gtttggcctc gtggcttgtg
      301 ctgaggcaga acatcatggt ggaaagccac gacccaccgt gatgaactgt actctctgag
      361 agatgagata aatcctccct tcaatttgcc tttgcaggtg acagcagcat ggctgcgtcc
      421 cagaccttcc cactggggcc tacccatgag cctgcaagcg ccctgatgga gcctctgcct
      481 tgtacacgaa gcttggctga gggcttcctg gaggaggagc ttcggctcaa tgccgagctg
      541 agccagctgc agttcccgga gcccgtgggt gtcatctaca acccggtgga ttatgcttgg
      601 gagccacacc gtaactatgt gactcgctac tgccaaggcc ccaaggaagt gctgttcttg
      661 ggcatgaacc caggaccttt tggcatggcc caaacagggg taccctttgg ggaagtgaat
      721 gtggtccggg actggttggg cgttgggggc cctgtgctga cccctccaca agagcacccc
      781 aagcgaccag tgttgggact ggagtgccca cagtcagagg tgagcggagc ccgattctgg
      841 ggctttttcc ggaccctctg cggacagcct caagtcttct tccggcactg ctttgtccac
      901 aatctgtgtc ctctactctt cttggctccc agtggacgaa accttacccc agctgagctg
      961 cccgccaagc agcgggagca gctgctgtcg atctgcgacg cagccctctg ccggcaggtg
     1021 cagctgctag gggtgcgtct ggtagtggga gtggggcggc tggcagagca gcgagcccga
     1081 agagctctag cagggctgac cccggaggtg caggtggagg ggctcctgca tccatctcct
     1141 cgaagcgcac aggccaacaa aggctgggag gcggcagcca gggaaagact ccaggagttg
     1201 gggctgctgc ctctgctaac ggatgagggt tcagccagac ctacatagta gagaccccag
     1261 ccagtgttgc attcagcctg ctggactgtc ctctaatggc ccagggtggg cagctctcct
     1321 gctgtcttcc ccttcccttg agcctctcac ccttttgtct ccagtgtctg gtcatgcacg
     1381 gcatcagcag agggttgggc ctgtgcactc cacctaccac atccttgcct tgggagtctc
     1441 tgtgtgcagt gcagagtcct aggtcgggat gtctgaagtc atattcgcag ttttggagga
     1501 aagatggttt ttgttttaat ctttcctgtt ttgcttttta ttggcatcat gtttggcatc
     1561 gtgtcatgta ctctagaaat gttctctaat gctgatctac ctcagccttt tttgacacta
     1621 actacatact agtggggttc agtgtgaggt ttccacacag gcattcagcg tacaccgatg
     1681 gagtccaacc cccttttatc tgtcctctcc acacttcctc ttccccagtc agtggacaat
     1741 ctttccagga tccctgctca tccttacaga tcatctggca gactcctgta atcccataac
     1801 tcagaggcag gaagatcagg agtccgaggc cgccttcagc tacatattga gttcaaggcc
     1861 agcttggaac acattagtct ctgtctcaaa aataagtaag taaataagta ataaaaaagt
     1921 gaacttcttc agggagccca gagctgtctg agaagctgta tctgagggag acttctgtgt
     1981 gtctagacta ggaaggatct agattttcat ctctcccttt ttttgctccc tataaggcct
     2041 ttgaacttaa aaaaaaatgt atctatttat tttggttttt cgaggcaggg tttctctgtg
     2101 ttgcccttac tgtcctagaa ctcactttgt agaccgggtt ggcctcaaac tcagagaact
     2161 tatattttta aaaaaaatcc ctctgttttg tttctgttca cgttgtctat ctgtttttaa
     2221 atttcgaaac aaggtctcat tgtgttggcc tgtaactcat tattatagac cagctccgct
     2281 ttgacctcac aagaaatctg cctacctctg cctcctgagt gctgggatta aaaggatgtg
     2341 ccaccctgcc tgacctgctt atgaacaacc tctctaagca ctaagatcct ttaaaaaaaa
     2401 gagggtgtgc tgcctcctag cactggtgtg aggtgtgagg atcgactgag ataacactgc
     2461 agagtgcttt gcaaaaggct gacatatggt cctcactaca cactctttct gctcacctcc
     2521 cttcgaggtg gggttatatc aatgcactac aagggaattt cccgtctgct ctgaagacct
     2581 ttgcctataa ccctgctcgc ttgctggaga atgaccaatg ttacgagaaa cttagcctct
     2641 gacttttcag actaagcatt aaagactcct aagccttgtg ttgggtctct cctctcttcc
     2701 tacacttcat tggtgggaga atgattttag gttatctcct cccttttctt acctattcac
     2761 tgtctgaaaa aacttttctc aggtagcttg aggagggggg acagagacct ctgattgccc
     2821 tagccaagct gcaggggttt tggtaggggc ctgccctgaa gttcatcagg tactagggca
     2881 actgttggga acaatgagga gctggtgtct catctgtctc tttaatggtt ggttggatga
     2941 atctgtggta agggctggga agtgagccac agtgagagca gcaggtctta gggccatctt
     3001 aatgaagata catggtctct ctatctcctg ggatacaagt catctccagc attctaaaaa
     3061 ttagtatatt tctatctcag ctccagagag ggagactaaa cctttgagga ttccccagtg
     3121 atagaagtgt tgtaattatt catgttgggg gtctcagagc attccagaat ttgttgaact
     3181 ttgactcagg atggaggcca accctccaga aagaccagcc gtatgtattg agggttgggg
     3241 cttagcctga atgccagaat gggacaagga tagagattaa gttcagccac atggcccatg
     3301 atctcgacca gttatacctc cacagtgacc ctagccagct ctcttggaca cttgagtttc
     3361 cactaagctt gctggctggt gcacacatgt atatctgtgt ttctttttgg gtatttggtg
     3421 ctggtttgtg tattcattca ttcattcatt cattcatgtg ctctgtgtgt cattttggag
     3481 ttctgggcat caagcccaga attttgtgaa tgggaagcat gcactcttct atcactgagc
     3541 tacatccctt gatttgtatt tgtgataaat tgtaataaat aaataaacaa acacagtgtt
     3601 ttcccccctg a
//
