LOCUS       NM_021491               5125 bp    mRNA    linear   ROD 14-JAN-2025
DEFINITION  Mus musculus sphingomyelin phosphodiesterase 3, neutral (Smpd3),
            mRNA.
ACCESSION   NM_021491
VERSION     NM_021491.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5125)
  AUTHORS   Inskeep,K.A., Crase,B., Dayarathna,T. and Stottmann,R.W.
  TITLE     SMPD4-mediated sphingolipid metabolism regulates brain and primary
            cilia development
  JOURNAL   Development 151 (22) (2024)
   PUBMED   39470011
REFERENCE   2  (bases 1 to 5125)
  AUTHORS   Zhu,Z., McClintock,T.S. and Bieberich,E.
  TITLE     Transcriptomics analysis reveals potential regulatory role of
            nSMase2 (Smpd3) in nervous system development and function of
            middle-aged mouse brains
  JOURNAL   Genes Brain Behav 23 (4), e12911 (2024)
   PUBMED   39171374
  REMARK    GeneRIF: Transcriptomics analysis reveals potential regulatory role
            of nSMase2 (Smpd3) in nervous system development and function of
            middle-aged mouse brains.
REFERENCE   3  (bases 1 to 5125)
  AUTHORS   Touahri,Y., Hanna,J., Tachibana,N., Okawa,S., Liu,H., David,L.A.,
            Olender,T., Vasan,L., Pak,A., Mehta,D.N., Chinchalongporn,V.,
            Balakrishnan,A., Cantrup,R., Dixit,R., Mattar,P., Saleh,F.,
            Ilnytskyy,Y., Murshed,M., Mains,P.E., Kovalchuk,I., Lefebvre,J.L.,
            Leong,H.S., Cayouette,M., Wang,C., Del Sol,A., Brand,M., Reese,B.E.
            and Schuurmans,C.
  TITLE     Pten regulates endocytic trafficking of cell adhesion and Wnt
            signaling molecules to pattern the retina
  JOURNAL   Cell Rep 43 (4), 114005 (2024)
   PUBMED   38551961
REFERENCE   4  (bases 1 to 5125)
  AUTHORS   Butera,A., Agostini,M., Cassandri,M., De Nicola,F., Fanciulli,M.,
            D'Ambrosio,L., Falasca,L., Nardacci,R., Wang,L., Piacentini,M.,
            Knight,R.A., Jia,W., Sun,Q., Shi,Y., Wang,Y., Candi,E. and
            Melino,G.
  TITLE     ZFP750 affects the cutaneous barrier through regulating lipid
            metabolism
  JOURNAL   Sci Adv 9 (17), eadg5423 (2023)
   PUBMED   37115925
REFERENCE   5  (bases 1 to 5125)
  AUTHORS   Li,Q., Li,J., Wang,K., Liao,L., Li,Y., Liang,H., Huang,C., Gan,J.,
            Dong,X., Hu,Y., Cheng,J., Ji,H., Liu,C., Zeng,M., Yu,S., Wang,B.,
            Qian,J., Tang,Z., Peng,Y., Tang,S., Li,M., Zhou,J., Yan,J. and
            Li,C.
  TITLE     Activation of Sphingomyelin Phosphodiesterase 3 in Liver
            Regeneration Impedes the Progression of Colorectal Cancer Liver
            Metastasis Via Exosome-Bound Intercellular Transfer of Ceramides
  JOURNAL   Cell Mol Gastroenterol Hepatol 16 (3), 385-410 (2023)
   PUBMED   37245564
  REMARK    GeneRIF: Activation of Sphingomyelin Phosphodiesterase 3 in Liver
            Regeneration Impedes the Progression of Colorectal Cancer Liver
            Metastasis Via Exosome-Bound Intercellular Transfer of Ceramides.
REFERENCE   6  (bases 1 to 5125)
  AUTHORS   Hofmann,K., Tomiuk,S., Wolff,G. and Stoffel,W.
  TITLE     Cloning and characterization of the mammalian brain-specific,
            Mg2+-dependent neutral sphingomyelinase
  JOURNAL   Proc Natl Acad Sci U S A 97 (11), 5895-5900 (2000)
   PUBMED   10823942
REFERENCE   7  (bases 1 to 5125)
  AUTHORS   Sillence,D.O., Ritchie,H.E., Dibbayawan,T., Eteson,D. and Brown,K.
  TITLE     Fragilitas ossium (fro/fro) in the mouse: a model for a recessively
            inherited type of osteogenesis imperfecta
  JOURNAL   Am J Med Genet 45 (2), 276-283 (1993)
   PUBMED   8456819
REFERENCE   8  (bases 1 to 5125)
  AUTHORS   Muriel,M.P., Bonaventure,J., Stanescu,R., Maroteaux,P., Guenet,J.L.
            and Stanescu,V.
  TITLE     Morphological and biochemical studies of a mouse mutant (fro/fro)
            with bone fragility
  JOURNAL   Bone 12 (4), 241-248 (1991)
   PUBMED   1793673
REFERENCE   9  (bases 1 to 5125)
  AUTHORS   Brown,K.S., Hetzel,S.C. and Harne,L.C.
  TITLE     Craniofacial consequences of connective tissue disorders in mice
  JOURNAL   Birth Defects Orig Artic Ser 20 (3), 113-136 (1984)
   PUBMED   6391574
  REMARK    Review article
REFERENCE   10 (bases 1 to 5125)
  AUTHORS   Guenet,J.L., Stanescu,R., Maroteaux,P. and Stanescu,V.
  TITLE     Fragilitas ossium: a new autosomal recessive mutation in the mouse
  JOURNAL   J Hered 72 (6), 440-441 (1981)
   PUBMED   6801109
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC134615.5 and AC124544.4.
            
            On Feb 7, 2019 this sequence version replaced NM_021491.3.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC043077.1, AK076416.1 [ECO:0000332]
            RNAseq introns              :: partial sample support SAMN00849374,
                                           SAMN00849375 [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-154               AC134615.5         88903-89056
            155-241             AC134615.5         138649-138735
            242-1763            AC124544.4         18817-20338
            1764-1839           AC124544.4         25002-25077
            1840-1995           AC124544.4         25166-25321
            1996-2085           AC124544.4         27266-27355
            2086-2149           AC124544.4         27495-27558
            2150-2312           AC124544.4         29174-29336
            2313-5125           AC124544.4         29581-32393
FEATURES             Location/Qualifiers
     source          1..5125
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="8"
                     /map="8 53.12 cM"
     gene            1..5125
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /note="sphingomyelin phosphodiesterase 3, neutral"
                     /db_xref="GeneID:58994"
                     /db_xref="MGI:MGI:1927578"
     exon            1..154
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /inference="alignment:Splign:2.1.0"
     exon            155..241
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /inference="alignment:Splign:2.1.0"
     exon            242..1763
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /inference="alignment:Splign:2.1.0"
     CDS             447..2414
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /EC_number="3.1.4.12"
                     /note="nSMase-2; neutral sphingomyelinase 2; neutral
                     sphingomyelinase II"
                     /codon_start=1
                     /product="sphingomyelin phosphodiesterase 3"
                     /protein_id="NP_067466.1"
                     /db_xref="CCDS:CCDS22634.1"
                     /db_xref="GeneID:58994"
                     /db_xref="MGI:MGI:1927578"
                     /translation="MVLYTTPFPNSCLSALHAVSWALIFPCYWLVDRLLASFIPTTYE
                     KRQRADDPCCLQLFCTVLFTPVYLALLVAALPFAFLGFIFWSPLQSARRPYSYSRLED
                     KNPAGGAALLSEWKGTGAGKSFCFATANVCLLPDSLARLNNVFNTQARAKEIGQRIRN
                     GAARPQIKIYIDSPTNTSISAASFSSLVSPQGGDGSRAVPGSIKRTASVEYKGDGGRH
                     PSDEAANGPASGEQADGSLEDSCIVRIGGEEGGRPQEADDPAAGSQARNGAGGTPKGQ
                     TPNHNQRDGDSGSLGSPSASRESLVKARAGQDSGGSGEPGANSKLLYKTSVVKKAAAR
                     RRRHPDEAFDHEVSAFFPANLDFLCLQEVFDKRAAAKLKEQLHGYFEYILYDVGVYGC
                     HGCCNFKCLNSGLFFASRYPVMDVAYHCYPNGCSFDALASKGALFLKVQVGSTPQDQR
                     IVGYIACTHLHAPPEDSAVRCEQLDLLQDWLADFRKSTSSTSTANPEELVVFDVICGD
                     LNFDNCSSDDKLEQQHSLFTRYKDPCRLGPGEEKPWAIGTLLDTNGLYDEDVCTPDNL
                     QKVLESEEGRREYLAFPTSKSPGAGQKGRKDLLKGNGRRIDYMLHAEEGLCPDWKAEV
                     EEFSFITQLSGLTDHLPVAMRLMVSAGEEEA"
     exon            1764..1839
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /inference="alignment:Splign:2.1.0"
     exon            1840..1995
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /inference="alignment:Splign:2.1.0"
     exon            1996..2085
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /inference="alignment:Splign:2.1.0"
     exon            2086..2149
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..2312
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /inference="alignment:Splign:2.1.0"
     exon            2313..5125
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2685..2690
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /note="hexamer: AATACA"
     polyA_site      2715
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /note="major polyA site"
     regulatory      5103..5108
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /note="hexamer: AATAAA"
     polyA_site      5125
                     /gene="Smpd3"
                     /gene_synonym="4631433G07Rik; fro; Nsm2; nSMase2"
                     /note="major polyA site"
ORIGIN      
        1 gtgctgagtc cgagggaggc tccggatgcc agagccgcgg tcgctgtaac catcgccgcc
       61 gcgagagccg tctctaggaa gccccggtcg cgaggagctg ggaggcgccg cgtgcgctgc
      121 cccgcaccga gcgccctcct gtgcgccacc tgagaaaaga aaagaagaga agagaagaga
      181 agagaagaga agagaagaga agagaagaga agagaagaga aaaaaacaca acagttaaaa
      241 ggttttcagc agactccggt gtcccagcat ggccccttga agcgaaccat gactgtctgt
      301 gggacactcg ccactgatca tccagagtca catctcacca ggaggtgacc tctcctccat
      361 cctccacctg ccccctgcct gggacagtgc tgcggctgcc acggacacca tgtagtagag
      421 ccggctgcgg cgcccagtga gctgcaatgg ttttgtacac gacccccttt cctaatagct
      481 gtctgtctgc cctccacgcc gtgtcctggg ccctcatctt cccatgttac tggctggtgg
      541 accggctgtt ggcctctttc atacccacca cctatgagaa gcgccagagg gcagatgacc
      601 cctgctgcct gcagctgttc tgcacggtcc tcttcacgcc agtctacctg gctctgcttg
      661 tggctgcact gccctttgcc tttctcgggt tcatcttctg gtctccactg cagtctgccc
      721 gccggcccta ctcctactcc cggctagaag acaagaaccc agctggtggg gcagccctgc
      781 ttagtgaatg gaagggcacg ggggcaggca aaagcttttg cttcgccacg gccaatgtct
      841 gcctcctccc cgactcactc gcgaggctca ataatgtgtt caacactcaa gctcgggcca
      901 aagagattgg gcagagaatc cgcaatgggg ccgccaggcc tcagatcaag atctacatcg
      961 attctcccac caacacctcc atcagtgcag ccagcttcag cagcctggtg tcgccacagg
     1021 gtggtgatgg atccagagct gttcctggga gcattaagag gacagcctct gtggaataca
     1081 agggggatgg tgggcgtcac cccagcgacg aggctgccaa cggcccagcc tctggagaac
     1141 aggctgacgg aagccttgag gacagctgca ttgtgcgaat tggtggcgag gaaggaggtc
     1201 ggccacaaga agctgatgac cctgctgccg ggagccaagc caggaatggg gctggtggga
     1261 ccccaaaggg ccagacaccc aaccacaatc agcgagatgg ggattctggg agcctgggca
     1321 gcccctcagc ctccagggag tccttggtga aggcacgggc tgggcaagac agtggtggca
     1381 gcggagagcc aggggctaac agcaagctgc tgtataagac ctctgtggtg aagaaggcag
     1441 cagcccgcag gaggcgccac cctgatgaag cctttgacca cgaggtctca gccttcttcc
     1501 cggccaatct ggacttcctg tgcctgcagg aggtgtttga caagcgtgcg gctgctaagt
     1561 tgaaagagca gctacacggc tacttcgagt acatcctgta tgatgttggg gtctacggtt
     1621 gtcatggttg ctgcaatttc aaatgtctca acagcggtct cttcttcgcc agccgctacc
     1681 ctgtcatgga cgtggcctat cactgttacc ccaatgggtg cagcttcgat gccctggcct
     1741 ctaagggagc tctgtttctc aaggtgcagg tgggaagcac acctcaggac caaagaattg
     1801 ttgggtacat cgcctgcaca cacctgcatg ccccaccaga ggacagtgcc gtccggtgtg
     1861 agcagctgga cctgcttcag gactggctgg ctgatttccg aaaatctacc tcctcgacca
     1921 gcacagccaa ccccgaggag ctggtggtgt ttgacgtcat ctgtggagat ttgaactttg
     1981 ataactgctc ttctgatgac aaactggagc agcagcactc gctctttact cgctacaaag
     2041 acccctgccg ccttgggccc ggggaggaga agccttgggc cattggcacc ctgctggaca
     2101 caaacggtct ctatgatgag gatgtgtgta ccccggacaa ccttcaaaag gtgttggaga
     2161 gcgaggaagg ccgcagggag tatctggcct tccccaccag caagagccca ggtgctgggc
     2221 agaagggccg gaaggatctg ctgaagggca atggccgccg catcgactac atgctgcacg
     2281 ccgaggaggg gctgtgccct gactggaagg ctgaggtaga agaattcagt tttatcaccc
     2341 agctgtccgg cctgaccgac cacctccccg tggccatgcg gctgatggtg tctgcagggg
     2401 aagaggaggc atagaccagc catgccacgg tctcgagcag tagggcctct gccggccctt
     2461 caagccacag cccgtgcctg ggctgtgtgc cctccattga gcacccagtg ctgggggaag
     2521 gagggcaggg gacagggaag gacgagccca tcaatcccct gcaagcctgg gaaccaggct
     2581 gctctgtgcc tctgggccaa ctctgcggac agaggacttg ggccagcctg ggagcccccg
     2641 gctgcctgac cagtgccatt ctttcagtca cgattttcta cagaaataca cagcacaacc
     2701 tagtttgtaa cccaagggtt agtacgagga ccgggtttct gtactctttg tatctcttct
     2761 caagctttgt ccagggttca ttacttgtgt ctgctgccaa tgttgtctcg catgcctgca
     2821 ccctcgcatg cccactgccc gcatgccatg tgccacactg tagcacccac accccttgct
     2881 cgggcctcaa aggccaaact caaaacacaa aaaacacaat cagaaccagc caaagaagca
     2941 cttcctgggc acagctgcca gctctccccc ctccagcctg gtggggagtg attgacggct
     3001 gtcattacct gccagcccag ggctcttccc agggtcttgc attcaagggc gattacattt
     3061 taagaagaaa acagaaaaaa atgttacaca caaaatcacc cttcccttag ggtctgtaag
     3121 tcactgggta gggctgtgct ctttcccagc ccccacagca gggtccagct ggctctggcc
     3181 accccagcag ctcctatagg agactcatac cagctctggg gccctaacag gaaatccaag
     3241 agggcatcac ttttgtacac gaaggggacc cagaatataa gggggtgggg gttctgattt
     3301 tgaaactgac aggttcgtag ctccttctct cctacccaac atgtcctctt ctgtggggcc
     3361 ctttgggtta gcctcttcct gaggacttcc ctcatccatg tatggctgag ctcaggaacc
     3421 acttgcctgc ccccaagtca ggctgcgcct ctggcctccc aggcccaccc ttccacaatg
     3481 gctaccctgg ataaagtggc aggaaaccaa gatagaatgg cttctttacc actgcaagct
     3541 caggtggcca gccaacagcc actgcttgtc tagagcaggg ctgactccag acagcatcca
     3601 gaagccactc ttgccaacgt gtgactagag ccagtcccaa gctcagcaca gtaaggctgc
     3661 tagaagccca ctgctctcca cctggtcctg ctcaggtgcc caagagcact gatcctgccg
     3721 acctcccttc agagaacgga ggctgatccc tgaccacaca ggaagggacg acgtctgcct
     3781 caggctgctc agctactgat ttgagggact atttgggtat tcccaacggt ctcttcagag
     3841 tgctctggtc ttgtatctgg cctggcgatg atggagggag catcaggccc ctgtggttgg
     3901 gacctctaaa atgttggtgc cctgaagctg tgccttgtca cccaccaagt agcaagaggg
     3961 atccagaggg gcacacattt gtcagacgcc aggctgtgct gagcagtgac cttcagagta
     4021 tgggcagttt agtattaggg catggatagc tggagacact ggtctcttaa cctcttggcc
     4081 tagagaaccc tgcccatgct ccagtgtcca gagctaggac agccttgctg ggttctctgc
     4141 tgtgcccctc ttctgccagg gcctggcctc ccaggaatct gggaagccct catggagtaa
     4201 aaggccccta actcctggaa acaaccagga tcctatccat tcaaggtacc atggggcagg
     4261 tccaatcttc aaaccaatgg cactgtgcac aaggagagta gaggaggccg aggccaccgt
     4321 cagggtccct aaacctgggt tagggtaaaa tgtttggagt aagagccgca tccttctctc
     4381 cttcccaact cccagggcag ccttaggcaa ggtgtactgc cctgtgcctc tgttatcaca
     4441 aggtcacctc tacacggtcc ggttcttgaa tccagaggag ggacagcctg cctgccttca
     4501 ggtcccctac acatctcagc actgaggcca tgcccgtctg ccacacactc cgctgtggtt
     4561 tgcctgtaga ctcagggttc ctgtactgcc aggcctttct atttcaactt ttaaaacaga
     4621 agaggcgttg ctaggatccc agactctctg acatctaagg ccgacacagg gatcacctga
     4681 ctcctaaccg gctctgcctg cccaggacct ctcaagggaa gcatagcgaa gtgtactagc
     4741 ttagcaagga ccattcaatt catctttatt tgtccaacac cacctggagt tttaagtatg
     4801 aatattaacc ttgatgcttt ttaactattg tattaacttg ctcagattta gaaatactgt
     4861 tttaaaagac aaagaagaaa cttttttaat ttctgtattt tttttctgta ttgtatccta
     4921 tgggacacta ggggttttat atggtaagac acccaaggtt ttggtaaaac attatcaagt
     4981 atatatccag acaattcttc cctagaagaa aaaacaatct ctatgcctga tttaaaaaaa
     5041 aagtcgaaaa gaggtggatt ttccctttat ggtgctgaaa ggaagacgga gactgaggag
     5101 aaaataaacc cgtgaggagg ctcaa
//
