LOCUS       NM_145533               2190 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus spermine oxidase (Smox), transcript variant 2, mRNA.
ACCESSION   NM_145533
VERSION     NM_145533.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2190)
  AUTHORS   Wang,G., Li,Z., Lin,P., Zhang,H., Wang,Y., Zhang,T., Wang,H.,
            Li,H., Lin,L., Zhao,Y., Jia,L., Chen,Y., Ji,H., Zhao,W., Fu,Z. and
            Zhong,Z.
  TITLE     Knockdown of Smox protects the integrity of the blood-brain barrier
            through antioxidant effect and Nrf2 pathway activation in stroke
  JOURNAL   Int Immunopharmacol 126, 111183 (2024)
   PUBMED   37984250
  REMARK    GeneRIF: Knockdown of Smox protects the integrity of the
            blood-brain barrier through antioxidant effect and Nrf2 pathway
            activation in stroke.
REFERENCE   2  (bases 1 to 2190)
  AUTHORS   Gobert,A.P., Latour,Y.L., Asim,M., Barry,D.P., Allaman,M.M.,
            Finley,J.L., Smith,T.M., McNamara,K.M., Singh,K., Sierra,J.C.,
            Delgado,A.G., Luis,P.B., Schneider,C., Washington,M.K.,
            Piazuelo,M.B., Zhao,S., Coburn,L.A. and Wilson,K.T.
  TITLE     Protective Role of Spermidine in Colitis and Colon Carcinogenesis
  JOURNAL   Gastroenterology 162 (3), 813-827 (2022)
   PUBMED   34767785
  REMARK    GeneRIF: Protective Role of Spermidine in Colitis and Colon
            Carcinogenesis.
            Erratum:[Gastroenterology. 2022 Jun;162(7):2138-2143. PMID:
            35461802]
REFERENCE   3  (bases 1 to 2190)
  AUTHORS   Bedogni,F. and Hevner,R.F.
  TITLE     Cell-Type-Specific Gene Expression in Developing Mouse Neocortex:
            Intermediate Progenitors Implicated in Axon Development
  JOURNAL   Front Mol Neurosci 14, 686034 (2021)
   PUBMED   34321999
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2190)
  AUTHORS   Reinoso-Sanchez,J.F., Baroli,G., Duranti,G., Scaricamazza,S.,
            Sabatini,S., Valle,C., Morlando,M., Casero,R.A. Jr., Bozzoni,I.,
            Mariottini,P., Ceci,R. and Cervelli,M.
  TITLE     Emerging Role for Linear and Circular Spermine Oxidase RNAs in
            Skeletal Muscle Physiopathology
  JOURNAL   Int J Mol Sci 21 (21), 8227 (2020)
   PUBMED   33153123
  REMARK    GeneRIF: Emerging Role for Linear and Circular Spermine Oxidase
            RNAs in Skeletal Muscle Physiopathology.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2190)
  AUTHORS   Zahedi,K., Brooks,M., Barone,S., Rahmati,N., Murray Stewart,T.,
            Dunworth,M., Destefano-Shields,C., Dasgupta,N., Davidson,S.,
            Lindquist,D.M., Fuller,C.E., Smith,R.D., Cleveland,J.L.,
            Casero,R.A. Jr. and Soleimani,M.
  TITLE     Ablation of polyamine catabolic enzymes provokes Purkinje cell
            damage, neuroinflammation, and severe ataxia
  JOURNAL   J Neuroinflammation 17 (1), 301 (2020)
   PUBMED   33054763
  REMARK    GeneRIF: Ablation of polyamine catabolic enzymes provokes Purkinje
            cell damage, neuroinflammation, and severe ataxia.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2190)
  AUTHORS   Bianchi,M., Amendola,R., Federico,R., Polticelli,F. and
            Mariottini,P.
  TITLE     Two short protein domains are responsible for the nuclear
            localization of the mouse spermine oxidase mu isoform
  JOURNAL   FEBS J 272 (12), 3052-3059 (2005)
   PUBMED   15955064
  REMARK    GeneRIF: we identified a second domain that is necessary for
            nuclear localization of the spermine oxidase splice variant
REFERENCE   7  (bases 1 to 2190)
  AUTHORS   Chaturvedi,R., Cheng,Y., Asim,M., Bussiere,F.I., Xu,H.,
            Gobert,A.P., Hacker,A., Casero,R.A. Jr. and Wilson,K.T.
  TITLE     Induction of polyamine oxidase 1 by Helicobacter pylori causes
            macrophage apoptosis by hydrogen peroxide release and mitochondrial
            membrane depolarization
  JOURNAL   J Biol Chem 279 (38), 40161-40173 (2004)
   PUBMED   15247269
REFERENCE   8  (bases 1 to 2190)
  AUTHORS   Cervelli,M., Bellini,A., Bianchi,M., Marcocci,L., Nocera,S.,
            Polticelli,F., Federico,R., Amendola,R. and Mariottini,P.
  TITLE     Mouse spermine oxidase gene splice variants. Nuclear subcellular
            localization of a novel active isoform
  JOURNAL   Eur J Biochem 271 (4), 760-770 (2004)
   PUBMED   14764092
REFERENCE   9  (bases 1 to 2190)
  AUTHORS   Cervelli,M., Polticelli,F., Federico,R. and Mariottini,P.
  TITLE     Heterologous expression and characterization of mouse spermine
            oxidase
  JOURNAL   J Biol Chem 278 (7), 5271-5276 (2003)
   PUBMED   12458219
  REMARK    GeneRIF: expression and characterization of mouse spermine oxidase
REFERENCE   10 (bases 1 to 2190)
  AUTHORS   Vujcic,S., Diegelman,P., Bacchi,C.J., Kramer,D.L. and Porter,C.W.
  TITLE     Identification and characterization of a novel flavin-containing
            spermine oxidase of mammalian cell origin
  JOURNAL   Biochem J 367 (Pt 3), 665-675 (2002)
   PUBMED   12141946
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BQ895563.1, AK143129.1 and BQ174872.1.
            
            [WARNING] On Jul 19, 2024 this sequence was replaced by
            NM_145533.3.
            
            On Apr 29, 2010 this sequence version replaced NM_145533.1.
            
            Transcript Variant: This variant (2) lacks an alternate, in-frame,
            exon, compared to variant 1. The resulting protein (isoform b) is
            shorter when it is compared to isoform a. This transcript and
            protein have also been called 'polyamine oxidase-l'.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK143129.1, BC004831.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-27                BQ895563.1         1-27
            28-2154             AK143129.1         2-2128
            2155-2190           BQ174872.1         1-36                c
FEATURES             Location/Qualifiers
     source          1..2190
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 63.45 cM"
     gene            1..2190
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /note="spermine oxidase"
                     /db_xref="GeneID:228608"
                     /db_xref="MGI:MGI:2445356"
     exon            1..175
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /inference="alignment:Splign:2.1.0"
     exon            176..409
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /inference="alignment:Splign:2.1.0"
     CDS             202..1869
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /EC_number="1.5.3.16"
                     /note="isoform b is encoded by transcript variant 2;
                     polyamine oxidase 1; PAO-1"
                     /codon_start=1
                     /product="spermine oxidase isoform b"
                     /protein_id="NP_663508.1"
                     /db_xref="CCDS:CCDS16763.1"
                     /db_xref="GeneID:228608"
                     /db_xref="MGI:MGI:2445356"
                     /translation="MQSCESSGDSADDPLSRGLRRRGQPRVVVIGAGLAGLAAARALL
                     EQGFTDVTVLEASSHIGGRVQSVRLGDTTFELGATWIHGSHGNPIYQLAEANGLLEET
                     TDGERSVGRISLYSKNGVACYLTNRGCRIPKDVVEEFSDLYNEVYNMTQEFFRHGKPV
                     NAESQNSVGVFTREKVRNRIRDDPDDTEATKRLKLAMIQQYLKVESCESSSHSIDEVS
                     LSAFGEWTEIPGAHHIIPSGFMRVVELLAEGIPPHVIQLGKPVRCIHWDQASAHPRGP
                     EIEPRGEGDHNHDTGEGGQSGENPQQGRWDEDEPWPVVVECEDCEVIPADHVIVTVSL
                     GVLKRQYTSFFRPCLPTEKVAAIHRLGIGTTDKIFLEFEEPFWGPECNSLQFVWEDEA
                     ESCTLTYPPELWYRKICGFDVLYPPERYGHVLSGWICGEEALVMERCDDEAVAEICTE
                     MLRQFTGNPNIPKPRRILRSAWGSNPYFRGSYSYTQVGSSGADVEKLAKPLPYTESSK
                     TAPMQVLFSGEATHRKYYSTTHGALLSGQREAARLIEMYRDLFQQGP"
     exon            410..636
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /inference="alignment:Splign:2.1.0"
     exon            637..810
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /inference="alignment:Splign:2.1.0"
     exon            811..1570
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /inference="alignment:Splign:2.1.0"
     exon            1571..1731
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /inference="alignment:Splign:2.1.0"
     exon            1732..2172
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2136..2141
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /note="hexamer: AATAAA"
     polyA_site      2158
                     /gene="Smox"
                     /gene_synonym="B130066H01Rik; PAO; PAOh1; SMO"
                     /note="major polyA site"
ORIGIN      
        1 aggcggggtg ggccggggac gcgaggcgga gcggggcccc cgcacaggcc ccggcggctg
       61 gctcggccct acggtcccgg cggcggctgc aggaggaagc ccagtggctg cggaggaggg
      121 gaggcggaga ggccgaggcc gctcgcgaag acgctcccgg gctccgcaag gaaaggctcc
      181 tagaaggtga gcacggacgg catgcaaagt tgtgaatcca gtggcgacag tgcggatgac
      241 cctctcagtc gtggcctacg gagaagggga cagcctcgtg tggtggtgat cggtgctggc
      301 ttggctggcc tggctgcagc tagagccctt ctggagcagg gcttcacgga tgtcactgtg
      361 cttgaggctt ccagccacat tgggggccgt gtgcagagtg tgaggcttgg agacaccacc
      421 tttgagctgg gagccacctg gatccatgga tcccacggga atcctatcta tcaactagca
      481 gaagccaatg gccttttgga agagacaaca gatggggagc gcagtgtggg ccgcatcagc
      541 ctttactcca agaatggcgt ggcctgctac cttaccaacc gtggctgccg catccccaag
      601 gacgtggttg aggaattcag cgatttatac aacgaggtct ataacatgac ccaggagttc
      661 ttccggcatg gtaaaccagt caatgccgag agtcagaaca gcgtcggggt gttcacccgg
      721 gagaaggtgc ggaatcgcat cagggatgac cctgacgaca cagaggccac caagcgcctg
      781 aagctcgcca tgatccagca gtacctgaag gtggagagct gtgagagcag ctcccacagc
      841 atagatgagg tgtccctgag cgcctttgga gaatggacgg agatcccagg cgcccatcac
      901 atcatcccct cgggcttcat gcgagttgtg gagctgctgg ctgagggcat tcctccacat
      961 gtcatccagt tggggaagcc ggtccgttgc atccactggg accaggcctc ggctcacccc
     1021 cggggtcctg agatcgagcc ccgtggtgag ggtgatcaca atcacgacac tggggagggt
     1081 ggccagagtg gagagaatcc gcagcagggg aggtgggacg aggatgagcc gtggcctgta
     1141 gtcgtggagt gcgaggattg cgaggtgatc ccagcggacc acgtgattgt gaccgtttcg
     1201 ctgggcgtgc tcaagaggca gtacaccagt ttctttaggc catgcctgcc cacggagaag
     1261 gtggccgcca tccaccgcct gggcattggt accactgaca agatcttcct tgaatttgag
     1321 gagccctttt ggggccccga gtgcaacagc ctgcagttcg tgtgggagga tgaggcagag
     1381 agctgtaccc tcacctaccc acctgagctc tggtaccgca agatctgtgg cttcgatgtc
     1441 ctttatccgc cagagcgcta tggccatgtg ctgagtggct ggatctgtgg ggaggaggct
     1501 cttgtcatgg agaggtgcga tgacgaggct gtagctgaga tctgcacaga gatgcttcga
     1561 cagttcacag ggaaccccaa tataccaaaa cctaggcgaa tcctgcgctc agcctggggc
     1621 agcaacccat acttccgggg ttcctattcc tacacacagg tgggctcaag tggggcggat
     1681 gtggagaagc tagccaagcc cctgccctac acagagagct ccaagacagc gcccatgcag
     1741 gtgctcttct ccggggaggc cacacaccgc aagtactact ccaccaccca cggtgctctg
     1801 ctctctggcc agcgcgaggc cgcccggctc atcgagatgt accgagacct cttccagcag
     1861 gggccctgaa aggtgtcctc actgccaaat gtgttccttg aagaaccact aactcgtgac
     1921 tgctggtcct cgtcccttgc ccccgtgctc ttaatttcct aattgtctgt agaatggaat
     1981 tttaccttct gtagtggaca ccgggctgac gtcagacctg gcccgagagc ttttcttttt
     2041 ctccaggctg ggtaatgacc aggtggggtt ctgcttcctg cttttttcct ctctcatgtt
     2101 attgcaagtg ccttcaatac tttgcatttt gggataataa aagaagtgac ccttcccacg
     2161 ctcctttgct tcaaaaaaaa aaaaaaaaaa
//
