LOCUS       NM_022316               3463 bp    mRNA    linear   ROD 10-FEB-2025
DEFINITION  Mus musculus SPARC related modular calcium binding 1 (Smoc1),
            transcript variant 2, mRNA.
ACCESSION   NM_022316
VERSION     NM_022316.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3463)
  AUTHORS   Ono,M., Nakajima,K., Tomizawa,S.I., Shirakawa,T., Okada,I.,
            Saitsu,H., Matsumoto,N. and Ohbo,K.
  TITLE     Spatial and temporal expression analysis of BMP signal modifiers,
            Smoc1 and Smoc2, from postnatal to adult developmental stages in
            the mouse testis
  JOURNAL   Gene Expr Patterns 54, 119383 (2024)
   PUBMED   39510490
REFERENCE   2  (bases 1 to 3463)
  AUTHORS   Li,X., Kempf,S., Delgado Lagos,F., Ukan,U., Popp,R., Hu,J.,
            Fromel,T., Gunther,S., Weigert,A. and Fleming,I.
  TITLE     A regulatory loop involving the cytochrome P450-soluble epoxide
            hydrolase axis and TGF-beta signaling
  JOURNAL   iScience 27 (10), 110938 (2024)
   PUBMED   39398242
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3463)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   4  (bases 1 to 3463)
  AUTHORS   Matoba,S., Kozuka,C., Miura,K., Inoue,K., Kumon,M., Hayashi,R.,
            Ohhata,T., Ogura,A. and Inoue,A.
  TITLE     Noncanonical imprinting sustains embryonic development and
            restrains placental overgrowth
  JOURNAL   Genes Dev 36 (7-8), 483-494 (2022)
   PUBMED   35483741
REFERENCE   5  (bases 1 to 3463)
  AUTHORS   Gamart,J., Barozzi,I., Laurent,F., Reinhardt,R., Martins,L.R.,
            Oberholzer,T., Visel,A., Zeller,R. and Zuniga,A.
  TITLE     SMAD4 target genes are part of a transcriptional network that
            integrates the response to BMP and SHH signaling during early limb
            bud patterning
  JOURNAL   Development 148 (23) (2021)
   PUBMED   34822715
REFERENCE   6  (bases 1 to 3463)
  AUTHORS   Manabe,R., Tsutsui,K., Yamada,T., Kimura,M., Nakano,I., Shimono,C.,
            Sanzen,N., Furutani,Y., Fukuda,T., Oguri,Y., Shimamoto,K.,
            Kiyozumi,D., Sato,Y., Sado,Y., Senoo,H., Yamashina,S., Fukuda,S.,
            Kawai,J., Sugiura,N., Kimata,K., Hayashizaki,Y. and Sekiguchi,K.
  TITLE     Transcriptome-based systematic identification of extracellular
            matrix proteins
  JOURNAL   Proc Natl Acad Sci U S A 105 (35), 12849-12854 (2008)
   PUBMED   18757743
REFERENCE   7  (bases 1 to 3463)
  AUTHORS   Kudo,L.C., Karsten,S.L., Chen,J., Levitt,P. and Geschwind,D.H.
  TITLE     Genetic analysis of anterior posterior expression gradients in the
            developing mammalian forebrain
  JOURNAL   Cereb Cortex 17 (9), 2108-2122 (2007)
   PUBMED   17150988
REFERENCE   8  (bases 1 to 3463)
  AUTHORS   Gersdorff,N., Muller,M., Schall,A. and Miosge,N.
  TITLE     Secreted modular calcium-binding protein-1 localization during
            mouse embryogenesis
  JOURNAL   Histochem Cell Biol 126 (6), 705-712 (2006)
   PUBMED   16736127
  REMARK    GeneRIF: expression of SMOC-1 examined in the early embryo day 7,
            as well as in following embryonic stages on days 12, 14, 16 & 18;
            broad and organ-specific distribution suggests multifunctional
            roles of SMOC-1 during mouse embryogenesis
REFERENCE   9  (bases 1 to 3463)
  AUTHORS   Keng,V.W., Yae,K., Hayakawa,T., Mizuno,S., Uno,Y., Yusa,K.,
            Kokubu,C., Kinoshita,T., Akagi,K., Jenkins,N.A., Copeland,N.G.,
            Horie,K. and Takeda,J.
  TITLE     Region-specific saturation germline mutagenesis in mice using the
            Sleeping Beauty transposon system
  JOURNAL   Nat Methods 2 (10), 763-769 (2005)
   PUBMED   16179923
REFERENCE   10 (bases 1 to 3463)
  AUTHORS   Vannahme,C., Gosling,S., Paulsson,M., Maurer,P. and Hartmann,U.
  TITLE     Characterization of SMOC-2, a modular extracellular calcium-binding
            protein
  JOURNAL   Biochem J 373 (Pt 3), 805-814 (2003)
   PUBMED   12741954
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK040931.1 and AC124384.4.
            
            On Apr 2, 2009 this sequence version replaced NM_022316.1.
            
            Transcript Variant: This variant (2) uses an alternate in-frame
            splice site in the coding region compared to variant 1. This
            results in a shorter protein (isoform 2) compared to isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC031804.1, AF070470.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-778               AK040931.1         2-779
            779-1759            AK040931.1         813-1793
            1760-3463           AC124384.4         17129-18832
FEATURES             Location/Qualifiers
     source          1..3463
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="12"
                     /map="12 37.28 cM"
     gene            1..3463
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /note="SPARC related modular calcium binding 1"
                     /db_xref="GeneID:64075"
                     /db_xref="MGI:MGI:1929878"
     exon            1..351
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     CDS             256..1614
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; secreted modular calcium binding protein 1;
                     SPARC-related protein; SPARC-related modular
                     calcium-binding protein 1; SMOC-1; SPARC-related gene
                     protein"
                     /codon_start=1
                     /product="SPARC-related modular calcium-binding protein 1
                     isoform 2 precursor"
                     /protein_id="NP_071711.2"
                     /db_xref="CCDS:CCDS26017.1"
                     /db_xref="GeneID:64075"
                     /db_xref="MGI:MGI:1929878"
                     /translation="MLPARVRLLTPHLLLVLVQLSPAGGHRTTGPRFLISDRDPPCNP
                     HCPRTQPKPICASDGRSYESMCEYQRAKCRDPALAVVHRGRCKDAGQSKCRLERAQAL
                     EQAKKPQEAVFVPECGEDGSFTQVQCHTYTGYCWCVTPDGKPISGSSVQNKTPVCSGP
                     VTDKPLSQGNSGRKDDGSKPTPTMETQPVFDGDEITAPTLWIKHLVIKDSKLNNTNVR
                     NSEKVHSCDQERQSALEEARQNPREGIVIPECAPGGLYKPVQCHQSTGYCWCVLVDTG
                     RPLPGTSTRYVMPSCESDARAKSIEADDPFKDRELPGCPEGKKMEFITSLLDALTTDM
                     VQAINSAAPTGGGRFSEPDPSHTLEERVAHWYFSQLDSNSSDDINKREMKPFKRYVKK
                     KAKPKKCARRFTDYCDLNKDKVISLPELKGCLGVSKEGGSLGSFPQGKRAGTNPFIGR
                     LV"
     exon            352..517
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            518..630
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            631..730
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            731..778
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            779..835
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            836..916
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            917..1109
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            1110..1192
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            1193..1298
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            1299..1543
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            1544..1597
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     exon            1598..3463
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3440..3445
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /note="hexamer: AATAAA"
     polyA_site      3460
                     /gene="Smoc1"
                     /gene_synonym="2600002F22Rik; SRG"
                     /note="major polyA site"
ORIGIN      
        1 cccgcatcca acctgctgtc gccgcggcca cgccgagcgc agcaggcgcc acgcgcagag
       61 cacacgctcg cactccagct ccgggcctcc gccgttcatg accgtgaccc cgggcgcagg
      121 ctcctccagc ccctgccgct ggctcttcgg ccactcccag ctgctgcaag ggtccccacc
      181 tccccaagtg ctttgaagct agcctgagcc cccctgcccg ctgcccgctc gtggctgccc
      241 ggctccgctg gcaccatgct gcccgcgcgc gtccgtctgc tcacgcccca cttgctgctc
      301 gtgttggtgc agctgtcccc ggccggcggc caccgcacca ccggccccag gttcctaata
      361 agtgaccgtg accctccgtg caacccacac tgtcccagga ctcagcccaa gcccatctgc
      421 gcctctgacg gcaggtccta cgagtccatg tgtgagtacc agagagccaa gtgccgagac
      481 ccagccctgg ccgtggtcca tcgaggtcga tgcaaagatg ctggccagag caagtgtcgc
      541 ctggagaggg ctcaggcctt ggaacaggcc aagaagcctc aggaggctgt gtttgtccca
      601 gagtgtggcg aggatggttc cttcacacag gtgcagtgcc atacgtacac agggtactgc
      661 tggtgtgtca ccccagatgg caagcccatc agtggttctt ccgtgcagaa taaaactcct
      721 gtatgttcag gtccagttac cgacaagccc ttgagccagg gtaattcagg aagaaaagat
      781 gatggctcta aacccacgcc cacgatggag acccaacctg tgttcgatgg agatgaaatc
      841 acagccccca ccttatggat taagcacttg gtaatcaaag actccaagtt gaataacacc
      901 aacgtaagaa attcagagaa agtccattcc tgtgaccagg aaagacagag tgccctggaa
      961 gaggcgcggc agaatccccg agagggcatt gtgatccccg agtgtgctcc tggtgggctc
     1021 tataagccgg tgcaatgcca ccaatccaca ggctactgtt ggtgcgtcct agtagacaca
     1081 gggcgcccat tgccggggac ttccacacgc tatgtgatgc caagttgcga gagtgacgcc
     1141 agagccaaga gcatagaggc cgatgaccct ttcaaggaca gggagttgcc aggttgtcct
     1201 gaagggaaga agatggaatt tattaccagc ctgctggatg ccctcaccac agacatggtt
     1261 caggccatta actcagcagc gcccactgga ggtgggaggt tctcagagcc agaccccagc
     1321 cacaccctgg aggagcgagt ggcacactgg tacttcagcc agctggatag caacagcagc
     1381 gatgacatta acaagcggga gatgaagccg ttcaagcgct atgtaaagaa gaaagccaag
     1441 cccaagaaat gtgcccggcg cttcaccgac tactgcgacc tgaacaagga taaggtcatc
     1501 tcactgcctg agctgaaggg ctgcctgggt gttagcaaag aaggtggtag ccttggcagc
     1561 ttccctcagg gaaaacgagc aggcacaaac ccgttcatcg gacgccttgt ctaaagagtg
     1621 gaaaaagcaa aaggcccatg gaaactccga ggagacagga ctgaccgtca gacacctaac
     1681 cctcagcgct gcccgtggcc cagccacagt ctgtgtaaca taagtggtgc ccgccatgtt
     1741 tgcactttta ataactctta cgtgtgtggt ttgtttctgg ttccatttgt aaacaccaat
     1801 tatctaatac catagcagga acaggaaagg gaagaaaagc tgtttattct ctcttttatt
     1861 gttaaggttt tttggatctg ctactgacat cttttaggtt ggggggggcg agggacgggg
     1921 tcgagaagaa agagatttat atactgtata taaatatata tgtaaattgt atagatcttt
     1981 tgtacaggca tcgacatcgc tgtttgtccc tcccttctac ttcctctgga ctcccagtcc
     2041 aggtctctcc ttagcttccc caagtttcac tgtatatgga ctctgagaga atagatggga
     2101 gccctgtgct tgtgctgtgg atagcatgtt ttcttctggt gggaagaggg tactggtcca
     2161 ttagctgtca aagtccagtc acttggccat tggctgggta ggaggggagc ccagttcctg
     2221 tgctggccag catggatttc agatctccgg tggcccacat gtccatgcta tccagatccc
     2281 ttggcagtct acagtgaata atggagcaat ctctacctga aggggctgga tttggctgct
     2341 ttaaacatct ctacccatgt ttagcatgtc ccccgtgtag gacatgagag gtgagacccc
     2401 aagcatgtga ggaaaaggac acccagcacc agcagggttc tcctgcagca gcaggtgttg
     2461 ggcatctgag gagatggtga agaggactga agacctgaac cagcccttct gcaaacccac
     2521 aagagagcct ccacaagcat gtagctccca acactagagt ccaactaggt tttactttcg
     2581 tgctctccct ccatctcttg gatcctcacc gattgggtca gccaccttgt tggccaatcc
     2641 cggagttgct cttattcctc tggcttttct catggacttc cagaattgtt gaattgacgt
     2701 ggcgtccctt acttctgctt cctcagagga ataggagcga tgatccagct atagggaact
     2761 ctaggctttt gcacagagtg tcaccggaat tgcaccaggg cagatgagca actttttctg
     2821 tctaggtgac atgctgattc agaggtcctt cagtcagagc agcaagtgat acttagcagc
     2881 tcttccatgg tcagccatga tggcagtgag ggaggccagg actggttctt ttgctggctt
     2941 gctctcctca acctgccttc cctgggtttc catctcagta tcctgtaaat gcatccaccc
     3001 tggtaaaccc tgctctattt tgggtctgga aggcatggac cacataatgc tgcccacatg
     3061 tggggaacat agccttctcg gagcttgatt cgatgaagtc tcagctccag aaggttcccg
     3121 ctgtcctggt ggaagtcaca catgctgtcc catgatgacc agtaaccctg tcctgcatgg
     3181 taaggtggga agaacacctt ctcttggagc cttgaacaga aacaccgcac gcagcaggct
     3241 ggagctggat ggccctccca gcagactcca gtcccatttc ctggccagaa ggaaatgtgc
     3301 acagagaagc cacctttatg taggagttta gaatctgacc agccaacttc tctctcacag
     3361 atgggcgtag gctgtgctgt gtggttttcc cttggggagg cgggagcaag aaaaagtatt
     3421 tgtagcttgt tttataaaaa ataaaaatgg gtaaacttgg gca
//
