LOCUS       NM_176996               3987 bp    mRNA    linear   ROD 11-MAR-2025
DEFINITION  Mus musculus smoothened, frizzled class receptor (Smo), mRNA.
ACCESSION   NM_176996
VERSION     NM_176996.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Zhu,X., Xu,M., Portal,C., Lin,Y., Ferdinand,A., Peng,T.,
            Morrisey,E.E., Dlugosz,A.A., Castellano,J.M., Lee,V., Seykora,J.T.,
            Wong,S.Y., Iomini,C. and Millar,S.E.
  TITLE     Identification of Meibomian gland stem cell populations and
            mechanisms of aging
  JOURNAL   Nat Commun 16 (1), 1663 (2025)
   PUBMED   39955307
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Leybova,L., Biswas,A., Sharan,R., Trejo,B.M., Kim,K.,
            Soto-Muniz,Y., Jones,R.A., Phillips,B.K. and Devenport,D.
  TITLE     Radially patterned morphogenesis of murine hair follicle placodes
            ensures robust epithelial budding
  JOURNAL   Dev Cell 59 (24), 3272-3289 (2024)
   PUBMED   39413781
REFERENCE   3  (bases 1 to 3987)
  AUTHORS   Bian,F., Hansen,V., Feng,H.C., He,J., Chen,Y., Feng,K.,
            Ebrahimi,B., Gray,R.S., Chai,Y., Wu,C.L. and Liu,Z.
  TITLE     The G protein-coupled receptor ADGRG6 maintains mouse growth plate
            homeostasis through IHH signaling
  JOURNAL   J Bone Miner Res 39 (11), 1644-1658 (2024)
   PUBMED   39236220
REFERENCE   4  (bases 1 to 3987)
  AUTHORS   Jun,J.H., Du,K., Dutta,R.K., Maeso-Diaz,R., Oh,S.H., Wang,L.,
            Gao,G., Ferreira,A., Hill,J., Pullen,S.S. and Diehl,A.M.
  TITLE     The senescence-associated secretome of Hedgehog-deficient
            hepatocytes drives MASLD progression
  JOURNAL   J Clin Invest 134 (19), e180310 (2024)
   PUBMED   39190624
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3987)
  AUTHORS   Walker,M.F., Zhang,J., Steiner,W., Ku,P.I., Zhu,J.F.,
            Michaelson,Z., Yen,Y.C., Lee,A., Long,A.B., Casey,M.J., Poddar,A.,
            Nelson,I.B., Arveseth,C.D., Nagel,F., Clough,R., LaPotin,S.,
            Kwan,K.M., Schulz,S., Stewart,R.A., Tesmer,J.J.G., Caspary,T.,
            Subramanian,R., Ge,X. and Myers,B.R.
  TITLE     GRK2 kinases in the primary cilium initiate SMOOTHENED-PKA
            signaling in the Hedgehog cascade
  JOURNAL   PLoS Biol 22 (8), e3002685 (2024)
   PUBMED   39138140
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 3987)
  AUTHORS   St-Jacques,B., Dassule,H.R., Karavanova,I., Botchkarev,V.A., Li,J.,
            Danielian,P.S., McMahon,J.A., Lewis,P.M., Paus,R. and McMahon,A.P.
  TITLE     Sonic hedgehog signaling is essential for hair development
  JOURNAL   Curr Biol 8 (19), 1058-1068 (1998)
   PUBMED   9768360
REFERENCE   7  (bases 1 to 3987)
  AUTHORS   Hardcastle,Z., Mo,R., Hui,C.C. and Sharpe,P.T.
  TITLE     The Shh signalling pathway in tooth development: defects in Gli2
            and Gli3 mutants
  JOURNAL   Development 125 (15), 2803-2811 (1998)
   PUBMED   9655803
REFERENCE   8  (bases 1 to 3987)
  AUTHORS   Kasarskis,A., Manova,K. and Anderson,K.V.
  TITLE     A phenotype-based screen for embryonic lethal mutations in the
            mouse
  JOURNAL   Proc Natl Acad Sci U S A 95 (13), 7485-7490 (1998)
   PUBMED   9636176
REFERENCE   9  (bases 1 to 3987)
  AUTHORS   Akiyama,H., Shigeno,C., Hiraki,Y., Shukunami,C., Kohno,H.,
            Akagi,M., Konishi,J. and Nakamura,T.
  TITLE     Cloning of a mouse smoothened cDNA and expression patterns of
            hedgehog signalling molecules during chondrogenesis and cartilage
            differentiation in clonal mouse EC cells, ATDC5
  JOURNAL   Biochem Biophys Res Commun 235 (1), 142-147 (1997)
   PUBMED   9196051
REFERENCE   10 (bases 1 to 3987)
  AUTHORS   Stone,D.M., Hynes,M., Armanini,M., Swanson,T.A., Gu,Q.,
            Johnson,R.L., Scott,M.P., Pennica,D., Goddard,A., Phillips,H.,
            Noll,M., Hooper,J.E., de Sauvage,F. and Rosenthal,A.
  TITLE     The tumour-suppressor gene patched encodes a candidate receptor for
            Sonic hedgehog
  JOURNAL   Nature 384 (6605), 129-134 (1996)
   PUBMED   8906787
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC069469.5.
            
            On Oct 5, 2022 this sequence version replaced NM_176996.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC096028.1, FJ883552.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-873               AC069469.5         109218-110090
            874-1079            AC069469.5         127207-127412
            1080-1289           AC069469.5         128059-128268
            1290-1462           AC069469.5         128429-128601
            1463-1682           AC069469.5         128911-129130
            1683-1806           AC069469.5         129220-129343
            1807-1899           AC069469.5         130518-130610
            1900-2008           AC069469.5         130994-131102
            2009-2194           AC069469.5         132073-132258
            2195-2343           AC069469.5         132603-132751
            2344-2478           AC069469.5         133221-133355
            2479-3987           AC069469.5         133589-135097
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 12.36 cM"
     gene            1..3987
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="smoothened, frizzled class receptor"
                     /db_xref="GeneID:319757"
                     /db_xref="MGI:MGI:108075"
     exon            1..873
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     CDS             531..2912
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="bent body; smoothened homolog; protein smoothened"
                     /codon_start=1
                     /product="protein smoothened precursor"
                     /protein_id="NP_795970.3"
                     /db_xref="CCDS:CCDS19965.1"
                     /db_xref="GeneID:319757"
                     /db_xref="MGI:MGI:108075"
                     /translation="MAAGRPVRGPELAPRRLLQLLLLVLLGGPGRGAALSGNVTGPGP
                     HSASGSSRRDVPVTSPPPPLLSHCGRAAHCEPLRYNVCLGSALPYGATTTLLAGDSDS
                     QEEAHGKLVLWSGLRNAPRCWAVIQPLLCAVYMPKCENDRVELPSRTLCQATRGPCAI
                     VERERGWPDFLRCTPDHFPEGCPNEVQNIKFNSSGQCEAPLVRTDNPKSWYEDVEGCG
                     IQCQNPLFTEAEHQDMHSYIAAFGAVTGLCTLFTLATFVADWRNSNRYPAVILFYVNA
                     CFFVGSIGWLAQFMDGARREIVCRADGTMRFGEPTSSETLSCVIIFVIVYYALMAGVV
                     WFVVLTYAWHTSFKALGTTYQPLSGKTSYFHLLTWSLPFVLTVAILAVAQVDGDSVSG
                     ICFVGYKNYRYRAGFVLAPIGLVLIVGGYFLIRGVMTLFSIKSNHPGLLSEKAASKIN
                     ETMLRLGIFGFLAFGFVLITFSCHFYDFFNQAEWERSFRDYVLCQANVTIGLPTKKPI
                     PDCEIKNRPSLLVEKINLFAMFGTGIAMSTWVWTKATLLIWRRTWCRLTGHSDDEPKR
                     IKKSKMIAKAFSKRRELLQNPGQELSFSMHTVSHDGPVAGLAFDLNEPSADVSSAWAQ
                     HVTKMVARRGAILPQDVSVTPVATPVPPEEQANMWLVEAEISPELEKRLGRKKKRRKR
                     KKEVCPLRPAPELHHSAPVPATSAVPRLPQLPRQKCLVAANAWGTGESCRQGAWTLVS
                     NPFCPEPSPHQDPFLPGASAPRVWAQGRLQGLGSIHSRTNLMEAEILDADSDF"
     exon            874..1079
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1289
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            1290..1462
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..1682
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            1683..1806
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            1807..1899
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2008
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            2009..2194
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            2195..2343
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            2344..2478
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     exon            2479..3987
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3968..3973
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="hexamer: AATAAA"
     polyA_site      3987
                     /gene="Smo"
                     /gene_synonym="bnb; E130215L21Rik; Smoh; smoothened"
                     /note="major polyA site"
ORIGIN      
        1 attccagaga gcagggccag ctagagcaac aaaggagccc ggtagtcggc agggagagct
       61 cagggggctg cggcgcgctc gcgcggaggt ggctgctggg ccgcgggctg gcgtgggggc
      121 ggagccgggg acccactccc gcaccccccc cccccccccg gccggcgcct ggcctccatc
      181 gaggggctgg gagtcggttt taatggtggg agagggaatg gggctggaga tcggggcccc
      241 ggagggttcc cagggttgaa gacagcttcg atctccaggc cagggagtcc ggggtctgtg
      301 catcctggcc cgggcctgcg ctgctcaaca tggggcccgg gttccaaagt ttgcaaagtt
      361 gggagtcgag gggcccggac gcgcgcggcg cctggcgaaa gctggcccca gactttcggg
      421 gcgcaccggt cgcctaagta gcctccgcgg cccccggggt cgtgtgtgtg gccaggggac
      481 tccggggagc tccggggcgc ctcagctttt gctgagttgg ctgtttggcc atggccgctg
      541 gccgccccgt gcgtgggccc gagctggcgc cccggaggct gctgcagttg ctgctgctgg
      601 tactgctggg gggcccgggc cggggggcgg ccttgagcgg gaacgtgacc gggcctgggc
      661 ctcacagcgc cagcgggagc tcgaggaggg acgtgccggt gaccagccct ccgccgccgc
      721 tgctgagcca ctgcggccgg gccgcccact gcgagccttt gcgctacaac gtgtgcctgg
      781 gctcggcgct gccctacgga gccaccacca cgctgctggc tggggactcg gactcgcagg
      841 aggaagcgca cggcaagctc gtgctctggt ccggcctccg gaatgccccc cgctgctggg
      901 cagtgatcca gcccctgctg tgtgctgtct acatgcccaa gtgtgagaat gaccgagtgg
      961 agttgcccag ccgtaccctc tgccaggcca cccgaggccc ctgtgccatt gtggagcggg
     1021 agcgagggtg gcctgacttt ctgcgttgca caccggacca cttccctgaa ggctgcccaa
     1081 acgaggtaca aaacatcaag ttcaacagct caggccaatg tgaagcaccc ttggtgcgaa
     1141 cagacaaccc caagagctgg tatgaggacg tggagggctg tgggattcag tgtcagaacc
     1201 cgctgttcac cgaggccgag caccaggaca tgcacagcta catcgcagcc ttcggggcgg
     1261 tcactggtct ctgcacgctc ttcacactgg ccacctttgt ggctgactgg cggaactcca
     1321 atcgctaccc tgcggttatt ctcttctatg tcaatgcgtg tttcttcgtg ggcagcattg
     1381 gctggctggc ccagttcatg gatggtgccc gccgagagat tgtttgccga gcagatggca
     1441 ccatgagatt tggggagccc acctccagtg agaccctgtc ctgtgtcatc atctttgtca
     1501 ttgtgtacta tgccttgatg gctggagtag tctggttcgt ggtcctcacc tatgcctggc
     1561 acacctcctt caaagccctg ggcaccacct accagcctct ctcgggcaag acatcctatt
     1621 tccacctgct cacgtggtca ctcccctttg tcctcacggt ggcaatcctg gctgtggctc
     1681 aggtagatgg agactccgtg agtggcatct gttttgtagg ctacaagaac tatcggtacc
     1741 gtgctggctt tgtcctggcc ccaattggcc tggtgcttat tgtgggaggc tacttcctca
     1801 tcagaggggt catgactctg ttctccatca agagcaacca ccctgggctt ctgagtgaga
     1861 aggcagccag caagatcaac gagaccatgc tgcgcctggg catttttggc ttcctggcct
     1921 ttggctttgt gctcatcacc ttcagctgcc acttctatga cttcttcaac caggctgagt
     1981 gggagcgtag cttccgggac tatgtgctat gccaagccaa cgtgaccatc gggctgccta
     2041 ccaagaagcc cattcctgac tgtgagatca agaatcggcc cagcctcctg gtggagaaga
     2101 tcaatctatt tgccatgttt ggcactggca ttgccatgag cacctgggtc tggaccaagg
     2161 ccaccctgct catctggagg cgcacctggt gcaggttgac tgggcacagt gatgatgagc
     2221 ccaagagaat caagaagagc aagatgatcg ccaaggcctt ctctaagcgg cgtgagctgc
     2281 tgcagaaccc gggccaggag ctctccttca gcatgcacac tgtctcccat gatggacctg
     2341 ttgcgggttt ggcttttgac ctcaatgaac cctcagctga cgtctcctct gcctgggctc
     2401 agcatgtcac caagatggtg gctcggagag gagccatatt gccccaggat gtgtccgtta
     2461 cccctgtggc aactccagtg ccaccagaag aacaagccaa catgtggctg gttgaggcag
     2521 agatctcccc agagttagag aagcgtttgg gccggaagaa aaagcggagg aagaggaaga
     2581 aggaggtgtg ccccttgagg ccagcccctg agcttcacca ctctgcccct gttcctgcca
     2641 ccagtgcagt tcctcggctg cctcagctgc ctcggcagaa gtgcctggta gctgcaaacg
     2701 cctggggaac aggggagtcc tgccgacagg gagcctggac tctagtctcc aaccccttct
     2761 gcccagagcc tagtccccat caagatccat ttctccctgg tgcctcagcc ccccgggtct
     2821 gggctcaggg ccgcctccag gggctgggat ccattcattc ccgcactaac ctaatggagg
     2881 ctgagatctt ggatgcagac tcggacttct gagcctgcag ggcaggtcct aggatgggaa
     2941 agacaaatgt acacctttct atggctcttc ctgagagcac acctctgggt ctcatctgac
     3001 agagtctgtg ggccaagtgt ctgcctagaa gagctgtgta tgtctggcta gaagcagcca
     3061 ggccatggaa acaagttgaa tacagcgatt ggtaggcctc atgtcagaat caggaccctg
     3121 cacttcagga cccttgcttc tgcccaccaa tcagagtctg actggcagtg ttagtctccg
     3181 acagagcttg tactagggca ggaatggcag agacagggat gatggtaccc agagtgggct
     3241 gtggtggtct gtgaggtaac caagcccatg tctggcagat gagggctgtt tgcccttttc
     3301 tgtgccaatg agtgcccttt tctggcactc tcagaccaaa agtgtttatt gtgtcatttg
     3361 tcctttgtct aggagaggac aggactctct ttttcctctt cctggtagtt gtaatgacca
     3421 ctcccataag ggctataact gttcttcaca ggtggccctg ctcaaaatac atcctctctt
     3481 ttcccgttct atccctacat tcacatctca gttccactag gccaacctct tcctggttag
     3541 caccttaaaa ctgcagtgag cacacacaga cacacacaca catacactct cacacacaca
     3601 gacaggcatg cacacacaca cacacacaca cacacacaca cacacccctt acttctgagc
     3661 tctgtcttaa gagactactg gttcagctcc aggcctctga aagacatgtt atttcttcct
     3721 cacatccatc cagtggggag gaccctctga cttaagggac caccttggga agcttctgta
     3781 gcttcagcca ggcaagaaag cttcttccaa cttctgtttc tggtgggagc ggggggactc
     3841 ccacttttta caatgtctag tcattttcat agtgccccac attcaagaac cagacaacag
     3901 gatgccttag aagctggctg ggttttgggt caggggctca gtatgagaag aagaaatatg
     3961 aacagcaaat aaaacatttt tgtataa
//
