LOCUS       NM_001330757             739 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus small integral membrane protein 8 (Smim8), transcript
            variant 4, mRNA.
ACCESSION   NM_001330757 XM_006538161
VERSION     NM_001330757.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 739)
  AUTHORS   Oyama,Y., Miyata,H., Shimada,K., Fujihara,Y., Tokuhiro,K.,
            Garcia,T.X., Matzuk,M.M. and Ikawa,M.
  TITLE     CRISPR/Cas9-mediated genome editing reveals 12 testis-enriched
            genes dispensable for male fertility in mice
  JOURNAL   Asian J Androl 24 (3), 266-272 (2022)
   PUBMED   34290169
REFERENCE   3  (bases 1 to 739)
  AUTHORS   Koscielny,G., Yaikhom,G., Iyer,V., Meehan,T.F., Morgan,H.,
            Atienza-Herrero,J., Blake,A., Chen,C.K., Easty,R., Di Fenza,A.,
            Fiegel,T., Grifiths,M., Horne,A., Karp,N.A., Kurbatova,N.,
            Mason,J.C., Matthews,P., Oakley,D.J., Qazi,A., Regnart,J.,
            Retha,A., Santos,L.A., Sneddon,D.J., Warren,J., Westerberg,H.,
            Wilson,R.J., Melvin,D.G., Smedley,D., Brown,S.D., Flicek,P.,
            Skarnes,W.C., Mallon,A.M. and Parkinson,H.
  TITLE     The International Mouse Phenotyping Consortium Web Portal, a
            unified point of access for knockout mice and related phenotyping
            data
  JOURNAL   Nucleic Acids Res 42 (Database issue), D802-D809 (2014)
   PUBMED   24194600
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK021236.1, AK007660.1 and CO040081.1.
            
            On Sep 3, 2016 this sequence version replaced XM_006538161.2.
            
            Transcript Variant: This variant (4) uses an alternate splice site
            and lacks an alternate exon in the 5' UTR, compared to variant 1.
            Variants 1, 2, 3, and 4 encode the same protein.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC027508.1, AK007660.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849382, SAMN00849390
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-17                AK021236.1         2-18
            18-720              AK007660.1         2-704
            721-739             CO040081.1         1-19                c
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 16.86 cM"
     gene            1..739
                     /gene="Smim8"
                     /gene_synonym="1810030N24Rik; 2810406B13Rik"
                     /note="small integral membrane protein 8"
                     /db_xref="GeneID:66291"
                     /db_xref="MGI:MGI:1913541"
     exon            1..88
                     /gene="Smim8"
                     /gene_synonym="1810030N24Rik; 2810406B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            89..245
                     /gene="Smim8"
                     /gene_synonym="1810030N24Rik; 2810406B13Rik"
                     /inference="alignment:Splign:2.1.0"
     exon            246..725
                     /gene="Smim8"
                     /gene_synonym="1810030N24Rik; 2810406B13Rik"
                     /inference="alignment:Splign:2.1.0"
     regulatory      707..712
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smim8"
                     /gene_synonym="1810030N24Rik; 2810406B13Rik"
                     /note="hexamer: ATTAAA"
     polyA_site      725
                     /gene="Smim8"
                     /gene_synonym="1810030N24Rik; 2810406B13Rik"
                     /note="major polyA site"
ORIGIN      
        1 cagcagcggg cgttcgcacg ggacgccgga agtctctgag cctggcggcg tccgagctga
       61 agcttccccg gcttcccaat tgcccaaggt aataatcttc agtagccaag atgtcttcag
      121 cacctgatcc tccaacagtt aaaaaagaac cattaaaaga gaaaaacttt gaaaacccag
      181 gcctccgagg ggcgcacaca accaccttat ttcgagctgt gaatcccgag ctcttcatta
      241 aacccaacaa acctgtgatg gcttttggat tggtaaccct ttcactttgt gtggcttaca
      301 ttggttatct gcatgcaact caagagaaca gaaaggacct ctatgaagcc attgacagtg
      361 aagggcatcg gtacatgagg agaaaaacat ctaagtggga ctaactgctg cttccttcag
      421 gtggagtgtt attataggct ccgacacctt tgaaagaaag actgttagtg cacagaattg
      481 tttcttgttc cataatatgt taacaaggga gaatataaaa ttgaaagcag tccactgtgg
      541 tgagtttagt ctcattacag ctgaaggcat taaattctgt ataataaaag tacccagtac
      601 tcttccattt gcatggagtt tctaacgttt tagagtggat tgtgcctttg cagcaatgct
      661 ttactgttta ggagagaaga caaccccttc agttactaaa atcataatta aatgaaagaa
      721 taaaaaaaaa aaaaaaaaa
//
