LOCUS       NM_001160256            5867 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus SMG7 nonsense mediated mRNA decay factor (Smg7),
            transcript variant 2, mRNA.
ACCESSION   NM_001160256
VERSION     NM_001160256.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5867)
  AUTHORS   Steiner,A.J., Zheng,Y. and Tang,Y.
  TITLE     Characterization of a rhabdomyosarcoma reveals a critical role for
            SMG7 in cancer cell viability and tumor growth
  JOURNAL   Sci Rep 13 (1), 10152 (2023)
   PUBMED   37349371
  REMARK    GeneRIF: Characterization of a rhabdomyosarcoma reveals a critical
            role for SMG7 in cancer cell viability and tumor growth.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5867)
  AUTHORS   Okazaki,N., Kikuno,R., Ohara,R., Inamoto,S., Koseki,H., Hiraoka,S.,
            Saga,Y., Nagase,T., Ohara,O. and Koga,H.
  TITLE     Prediction of the coding sequences of mouse homologues of KIAA
            gene: III. the complete nucleotide sequences of 500 mouse
            KIAA-homologous cDNAs identified by screening of terminal sequences
            of cDNA clones randomly sampled from size-fractionated libraries
  JOURNAL   DNA Res 10 (4), 167-180 (2003)
   PUBMED   14621295
REFERENCE   3  (bases 1 to 5867)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC086651.1, BC082789.1 and AK129101.1.
            
            Transcript Variant: This variant (2) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (2)
            has a shorter and distinct N-terminus compared to isoform 1.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC086651.1, SRR9219381.196902.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMN00849374,
                                           SAMN00849375 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3705              BC086651.1         1-3705
            3706-5234           BC082789.1         3681-5209
            5235-5394           AK129101.1         5225-5384
            5395-5867           BC082789.1         5368-5840
FEATURES             Location/Qualifiers
     source          1..5867
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="1"
                     /map="1 65.07 cM"
     gene            1..5867
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /note="SMG7 nonsense mediated mRNA decay factor"
                     /db_xref="GeneID:226517"
                     /db_xref="MGI:MGI:2682334"
     exon            1..138
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     CDS             110..3526
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     protein SMG7; EST1-like protein C; Smg-7 homolog, nonsense
                     mediated mRNA decay factor; nonsense-mediated mRNA decay
                     factor SMG7"
                     /codon_start=1
                     /product="nonsense-mediated mRNA decay factor SMG7 isoform
                     2"
                     /protein_id="NP_001153728.1"
                     /db_xref="CCDS:CCDS48395.1"
                     /db_xref="GeneID:226517"
                     /db_xref="MGI:MGI:2682334"
                     /translation="MSLQSAQYLRQAEVLKAEMTDSKLGPAEVWTSRQALQDLYQKML
                     VTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFY
                     TQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHSSTIVKPQSSSCSYICQHCLVHLGD
                     IARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFP
                     FPAASTNLQKALSKALESRDELKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKL
                     EEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHSYSQDEQLCWTQLLAL
                     FMSFLGILCKCPLQNDSQESNNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWL
                     ISLLNSFHPREDDLSNTNATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQ
                     RRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQET
                     SVVESLATDGSPGLKSVLSTGRNPSNSCDSGEKPVVTFKENIKPREVNQGRSFPPKEV
                     KSQTELRKTPVSEARKTPVTQTPSQTSNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPP
                     PVAFSMGSGYTFPAGVSVPGTFLQSTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQ
                     GPQQSQPPSQPPLTSLPAQPTAQSTSQLQVQALAQQQQSPTKVIPALGKSPPHHSGFQ
                     QYQQADASKQLWNPPQVQSPLGKIMPVKQSYYLQTQDPIKLFEPSLQPPVIQQQPLEK
                     KMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRAVMAQQPNMDRRSKRSPGVFRPEQ
                     DPVPRMPFEDPKSSPLLPPDLLKSLAALEEEEELIFSNPPDLYPALLGPLASLPGRSL
                     FKSLLEKPSELMSHSSSFLSLTGFSVNQERYPNSSMFNEVYGKNLTTSSKAELNPSVA
                     SQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSAPFSNF
                     GPIGTPDNRDRRPADRWKTDKPAMGGFGVDYLSATSSSESSWHQASTPSGTWTGHGPS
                     MEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGAMNPPH"
     exon            139..170
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            171..288
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            289..421
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            422..593
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            594..665
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            666..816
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            817..952
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            953..1115
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            1116..1269
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1340
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1401
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            1402..1521
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            1522..1804
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            1805..1949
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            1950..2332
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            2333..2704
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            2705..2854
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            2855..2935
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            2936..3095
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            3096..3266
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            3267..3412
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     exon            3413..5803
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5780..5785
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /note="hexamer: ATTAAA"
     polyA_site      5803
                     /gene="Smg7"
                     /gene_synonym="9430023P16Rik; mKIAA0250"
                     /note="major polyA site"
ORIGIN      
        1 cggaggagag gaagatggcg gcgaccgcca gcacccgcgg ggccgcgggg ccgctccgag
       61 gagcctgaga gacccacgga ggcttcgcgg cgagacgcgg cggcggagga tgagcctgca
      121 gagcgcgcag tatctccggc aggcagaagt cctgaaggct gaaatgacag attctaagct
      181 gggtccggct gaggtctgga catccaggca agctctgcag gacttgtacc agaaaatgct
      241 agttactgat ctggaatacg ctttagacaa gaaagtagaa caggatctct ggaatcatgc
      301 ctttaagaat cagatcacaa cactgcaagg ccaagccaag aatcgagcaa acccaaatcg
      361 aagtgaagtc caggcaaacc tttctctgtt cctagaggca gctagcggct tctatactca
      421 gttattacag gaactgtgta cggtgtttaa tgtagatttg ccatgccgtg tgaagtcttc
      481 ccagttggga attattagca ataaacagac gcacagcagc accatagtga agccacagtc
      541 tagctcctgc tcctacattt gccaacactg cctcgtccac cttggagaca ttgctcgata
      601 cagaaatcag actagccagg cagagtccta ctataggcat gcagctcagc ttgtcccctc
      661 caatggtcag ccttacaatc agttggctat cttagcttct tccaaaggag accacctgac
      721 cacaattttc tactactgca gaagcattgc tgtgaaattc cctttcccag ctgcctccac
      781 taatctacaa aaagcacttt ctaaagcact ggaaagccgg gatgagttga aaaccaagtg
      841 gggtgtttct gacttcatca aggcctttat taaattccac ggtcatgtat acctgagtaa
      901 gagtttggaa aagctgagcc ctcttcgaga gaagttggaa gaacagttta agagactgct
      961 gttccaaaag gctttcaact ctcaacagtt agttcatgtc actgtcatta acctgtttca
     1021 acttcatcat cttcgtgact ttagcaatga aacagagcaa cacagttaca gccaagatga
     1081 acagctgtgt tggacacagt tgctggccct ctttatgtct tttctcggca tcctgtgcaa
     1141 atgtcctctc cagaatgatt ctcaggagtc caacaatgcc tacccccttc ctgcagtcaa
     1201 ggtctccatg gactggctaa gactccgacc tagagtcttt caagaagcag tggtggatga
     1261 aagacagtac atttggccct ggctaatttc tcttctaaat agtttccatc cccgtgaaga
     1321 tgatctctca aatactaatg ccacaccact tccagaggag tttgaattac aaggattctt
     1381 ggctttaaga ccttctttca ggaacttgga tttttccaaa ggccatcagg gtattacagg
     1441 agacaaagag ggtcaacaac gacgaatacg acagcagcgt ttgatctcca tagggaaatg
     1501 gattgctgat aaccagccac ggctgattca gtgtgaaaat gaggtaggga aattgttatt
     1561 tatcacagaa atcccagaat taatactaga agaccccagt gaagccaaag agaacctcat
     1621 cctacaagaa acatctgtgg tagagtcact ggctacggat gggagcccag gactgaaatc
     1681 agtgctgtct acaggccgaa atccaagcaa cagctgtgac tcaggagaga aaccagtggt
     1741 cacctttaaa gagaacatta agccacgaga agtgaaccaa ggaagaagct ttcctcccaa
     1801 agaggtaaaa tcccagacag aactaagaaa gactccagtg tctgaagcca ggaaaacgcc
     1861 tgtcactcaa actccaagtc aaacgagtaa ttctcagttc atccccatcc atcaccctgg
     1921 agccttccct cctcttccca gccgaccagg attcccgccc ccaacatatg ttatcccccc
     1981 tcctgtggca ttttctatgg gctcaggtta caccttccca gctggtgttt ctgtcccagg
     2041 aacctttctt cagtctacag ctcactctcc agcaggaaac caggtgcaag ctgggaaaca
     2101 gtcccacatt ccttacagcc agcaacggcc ctctggacca gggccaatga accagggacc
     2161 tcaacagtca cagccacctt cccagccacc ccttacatct ttaccagctc agccaacagc
     2221 acagtctaca agccaattgc aggttcaagc tctagctcag caacagcaat cccctacaaa
     2281 agtcatacca gctttgggga aaagcccgcc tcaccactct ggattccagc agtatcaaca
     2341 ggcagatgcc tccaaacagc tgtggaatcc ccctcaggtt caaagcccac tagggaaaat
     2401 tatgcctgtg aaacagtcct actaccttca gacccaagac cctataaaac tgtttgagcc
     2461 gtcattgcaa cctcctgtaa tacagcagca gcctctagag aaaaaaatga agcctttccc
     2521 catggagcca tataaccata atccctcaga agtcaaggtc ccagagttct actgggattc
     2581 ttcctacagc atggccgata acagagcagt aatggctcag caaccaaata tggaccgcag
     2641 gagcaaacgg tcacctggag tcttccgtcc agaacaggat cctgtgccca ggatgccatt
     2701 tgaggacccc aagagctccc ctctgcttcc tccggacctg ttaaagagtc tggctgcctt
     2761 ggaggaagag gaagagctga tcttttctaa ccctcctgat ctttacccag ctctgctggg
     2821 tcctctcgcc tctcttcctg gacgaagcct ctttaaatcc ttattggaga agccttctga
     2881 gctcatgtca cattcatctt ctttcctgtc cctcacgggg ttctctgtca atcaggaaag
     2941 atatccaaac agcagtatgt tcaatgaagt gtatggaaaa aacctgacaa ccagctccaa
     3001 ggcagaactg aacccctcag tcgcctccca ggaaacatca ctgtactccc tttttgaagg
     3061 gaccccatgg tctccatcac ttcctgccag ttcagatcat tcgacaccag ccagccagtc
     3121 tcctcattcc tctaacccaa gcagcctgcc cagttctcct ccaacgcata accataattc
     3181 tgctccattc tctaattttg gacccattgg gactccagat aacagggata ggcggcctgc
     3241 agataggtgg aaaactgata aaccagccat gggtggtttt ggtgttgatt atctctcagc
     3301 aacgtcatca tctgagagca gttggcatca ggctagcact ccgagtggca cctggacagg
     3361 ccacggcccc tccatggagg attcctctgc tgtcctcatg gaaagcctaa agtctatctg
     3421 gtccagttcc atgatgcatc ctgggccctc cgctctggag cagttgttaa tgcagcagaa
     3481 gcagaaacag cagcggggac aaggtgccat gaaccctcca cactgaggcc acaggagcaa
     3541 cccagggaat gaaggctcca taaaccatgg catgttgggt ttgcaggact gacccacaca
     3601 gtctctgcag gtggcagctc tcttgtctgt ttcttgctgt cgagagggtg taagtgttcc
     3661 accagcccgc tgagtgtgca cgaaatgttc gcagtgcaac aaaaagaaaa aaccatcagg
     3721 aactctcgtt cccccggggc tttccggagg gagagaggag ctgctgtttg tcccactcgg
     3781 ttactgatag cacgcctctc accttctcca ccgcgcatgt ccccaacacg tgggaagtgg
     3841 aagctgagaa gggaaggcag atgggagaaa ccaataggaa cttctcagtc cttttttcct
     3901 ctttggggaa taaaatagga atccattatg agtgctttgc tgactgagaa tgtagttgaa
     3961 attattctta aacatctttt attattactc tcagtagcaa aatatcacac tgaattcttc
     4021 catacacagg cgtatttcta gtcagtgtgg agcaaagaca gtcccgtttg ctgctcctgt
     4081 gagaggtcag tggtgacagg acagggaact gacctcttaa gccagtggga gtgtttcata
     4141 gggagggtac aaggcctctt agaaggttct gggagaggcc ttcctccagc ctggaaactc
     4201 cagcaggaaa tgcccttcac tctgtaggtg ctcgagcccc aatagaggat gcagtgtggg
     4261 tagtgctgct ggcctagacc agcagatggc tgctgtacga tttcaaattc atgtggtgga
     4321 ttcctacact ttacagtagc gtagcaagca gtctcagaag gcaggctagt tcattcacgg
     4381 cctggaaccc attttaatag gtggatgctt tcagtgtggt tctttttgtt gagttggttt
     4441 ttgtgtcccc atcctgcttc ccagcctctt gcatcagtct ccacccttta gccatatgct
     4501 gtaccgtagt cattgttttc ttcctaccgt gtacaacatc tcaccacgaa acagcagcat
     4561 ggggccagca cttggggccc aaaagcctga agtctgaaga ggaaggaagc ggcagtgcaa
     4621 gcgtcactgc agaggagccg ggctatgccc agtcacagct cctggccttc acctcgagga
     4681 tgaagttgaa ggccacagag acagatgcac actgtgcaca gaagaaaaca cagcagatgc
     4741 cactttggag agggcaagag aaaggaataa actctatttg ataatttata ttaggaggaa
     4801 agaggactga agatgttctg tgtaggaaca gaagaacgga cagcatttct gttagtcatt
     4861 tcctggaaaa gtaatatttt aatgggaaat tatggaaaca atctaaatgt ccaattgctg
     4921 tgctagggta gggattattt tctgggaggt gtgtgtgtgt gtgcgcgcgt gtgtgtccca
     4981 cacatggctt tctactctcc cagagggcaa gggctaagtg tgggaaatag tgtggagctt
     5041 agctgaagga cagctgtaga gcaaagcaca tccaggagcc ccaggtgtca ctggggtctg
     5101 ggcagccccg aaatgagatg gggtaaggta ttgctcattg ctcttcagaa agagtgcttg
     5161 aagccccagg cttactctat tgctctttta gtttgacatg gtatttggat tttttttctt
     5221 ttttcttttt ttttttgttt ttttgttttt ttgttttgtt ttttgggttt tggtttttgt
     5281 tttgttttgt ttttgaaagg tctgaaagtg aaacccttca ctaaatggca aaagaaactg
     5341 tctgtgctgc tccagtccct ccctgtgtcc atctttgtcc tctccctgtc ccttccctgc
     5401 taccttcacc cagtttgtgt atgtaagctc tgcattcaga cagctgcagc attccgaggt
     5461 tggaaatgtc actgattctt gcaccttaga ccagccaaca ggtttaccag ttccctccct
     5521 gcagtaccca cttcccagct atagccccag tctgcatgag aatttggtgt ttggaatgtt
     5581 tatgactctc tcggcggggt tcctcgcctt gccatcctca ctgtggggta atgaagaagg
     5641 ggaggagaat cttcatcaac tggttttgtg taataaactt tcgtgttttg ttttgatttg
     5701 atttgatttg gggttgtttt tcccccctgt ctgtctgtct gtgcaagatc tgcagctgct
     5761 gaaatcagct ttgcctttaa ttaaaccgtg ttctctccaa gcaaaaaaaa aaaaaaaaaa
     5821 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaa
//
