LOCUS       NM_133716               2923 bp    mRNA    linear   ROD 03-JUN-2024
DEFINITION  Mus musculus small ArfGAP 2 (Smap2), mRNA.
ACCESSION   NM_133716 XM_909722 XM_921411
VERSION     NM_133716.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Sumiyoshi,M., Masuda,N., Tanuma,N., Ogoh,H., Imai,E., Otsuka,M.,
            Hayakawa,N., Ohno,K., Matsui,Y., Hara,K., Gotoh,R., Suzuki,M.,
            Rai,S., Tanaka,H., Matsumura,I., Shima,H. and Watanabe,T.
  TITLE     Mice doubly-deficient in the Arf GAPs SMAP1 and SMAP2 exhibit
            embryonic lethality
  JOURNAL   FEBS Lett 589 (19 Pt B), 2754-2762 (2015)
   PUBMED   26296315
  REMARK    GeneRIF: either SMAP1 or SMAP2, is required for proper
            embryogenesis
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Funaki,T., Kon,S., Tanabe,K., Natsume,W., Sato,S., Shimizu,T.,
            Yoshida,N., Wong,W.F., Ogura,A., Ogawa,T., Inoue,K., Ogonuki,N.,
            Miki,H., Mochida,K., Endoh,K., Yomogida,K., Fukumoto,M., Horai,R.,
            Iwakura,Y., Ito,C., Toshimori,K., Watanabe,T. and Satake,M.
  TITLE     The Arf GAP SMAP2 is necessary for organized vesicle budding from
            the trans-Golgi network and subsequent acrosome formation in
            spermiogenesis
  JOURNAL   Mol Biol Cell 24 (17), 2633-2644 (2013)
   PUBMED   23864717
  REMARK    GeneRIF: study reveals a link between SMAP2 and CALM/syntaxin2 in
            clathrin-coated vesicle formation from the trans-Golgi network and
            subsequent acrosome formation
REFERENCE   3  (bases 1 to 2923)
  AUTHORS   Rorick,N.K., Kinoshita,A., Weirather,J.L., Peyrard-Janvid,M., de
            Lima,R.L., Dunnwald,M., Shanske,A.L., Moretti-Ferreira,D.,
            Koillinen,H., Kere,J., Mansilla,M.A., Murray,J.C., Goudy,S.L. and
            Schutte,B.C.
  TITLE     Genomic strategy identifies a missense mutation in WD-repeat domain
            65 (WDR65) in an individual with Van der Woude syndrome
  JOURNAL   Am J Med Genet A 155A (6), 1314-1321 (2011)
   PUBMED   21574244
REFERENCE   4  (bases 1 to 2923)
  AUTHORS   Funaki,T., Kon,S., Ronn,R.E., Henmi,Y., Kobayashi,Y., Watanabe,T.,
            Nakayama,K., Tanabe,K. and Satake,M.
  TITLE     Localization of SMAP2 to the TGN and its function in the regulation
            of TGN protein transport
  JOURNAL   Cell Struct Funct 36 (1), 83-95 (2011)
   PUBMED   21368446
  REMARK    GeneRIF: the trans-Golgi network (TGN) was verified as an
            additional site of SMAP2 localization based on its co-localization
            with various TGN-marker proteins.
REFERENCE   5  (bases 1 to 2923)
  AUTHORS   Natsume,W., Tanabe,K., Kon,S., Yoshida,N., Watanabe,T., Torii,T.
            and Satake,M.
  TITLE     SMAP2, a novel ARF GTPase-activating protein, interacts with
            clathrin and clathrin assembly protein and functions on the
            AP-1-positive early endosome/trans-Golgi network
  JOURNAL   Mol Biol Cell 17 (6), 2592-2603 (2006)
   PUBMED   16571680
REFERENCE   6  (bases 1 to 2923)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   7  (bases 1 to 2923)
  AUTHORS   Stryke,D., Kawamoto,M., Huang,C.C., Johns,S.J., King,L.A.,
            Harper,C.A., Meng,E.C., Lee,R.E., Yee,A., L'Italien,L.,
            Chuang,P.T., Young,S.G., Skarnes,W.C., Babbitt,P.C. and Ferrin,T.E.
  TITLE     BayGenomics: a resource of insertional mutations in mouse embryonic
            stem cells
  JOURNAL   Nucleic Acids Res 31 (1), 278-281 (2003)
   PUBMED   12520002
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC052413.1, BM944792.1 and BQ175680.1.
            
            On May 9, 2009 this sequence version replaced NM_133716.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC052413.1, BC031581.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164131, SAMN01164135
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1714              BC052413.1         1-1714
            1715-1716           BM944792.1         263-264
            1717-2898           BC052413.1         1715-2896
            2899-2923           BQ175680.1         1-25                c
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 56.7 cM"
     gene            1..2923
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /note="small ArfGAP 2"
                     /db_xref="GeneID:69780"
                     /db_xref="MGI:MGI:1917030"
     exon            1..499
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     CDS             397..1683
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /note="stromal membrane-associated GTPase-activating
                     protein 2"
                     /codon_start=1
                     /product="stromal membrane-associated protein 2"
                     /protein_id="NP_598477.2"
                     /db_xref="CCDS:CCDS18598.1"
                     /db_xref="GeneID:69780"
                     /db_xref="MGI:MGI:1917030"
                     /translation="MTGKSVKDVDRYQAVLANLLLEEDNKFCADCQSKGPRWASWNIG
                     VFICIRCAGIHRNLGVHISRVKSVNLDQWTQEQIQCMQEMGNGKANRLYEAYLPETFR
                     RPQIDPAVEGFIRDKYEKKKYMDRSLDINVLRKEKDDKWKRGNEPAPEKKMEPVVFEK
                     VKMPQKKEDAQLPRKSSPKSAAPVMDLLGLDAPVACSIANSKTSNALEKDLDLLASVP
                     SPSSVSRKAVGSMPTAGSAGSVPENLNLFPEPGSKSEETGKKQLSKDSILSLYGSQTP
                     QMPAQAMFMAPAQMAYPTAYPSFPGVTPPNSIMGGMVPPPVGMVAQPGASGMLTPMAM
                     PAGYMGGMQASMMGVPNGMMTTQQAGYMASMAAMPQTVYGVQPAQQLQWNLTQMTQQM
                     AGMNFYGANGMMNYGQSMGGGNGQAANQTLSPQMWK"
     exon            500..633
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     exon            634..719
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     exon            720..798
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     exon            799..885
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     exon            886..967
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     exon            968..1074
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1075..1240
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1241..1557
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     exon            1558..2905
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1679..1684
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /note="hexamer: AATAAA"
     polyA_site      1699
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /note="major polyA site"
     regulatory      2880..2885
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /note="hexamer: AATAAA"
     polyA_site      2900
                     /gene="Smap2"
                     /gene_synonym="1810031K02Rik; Smap1l"
                     /note="major polyA site"
ORIGIN      
        1 agagctgccg gaagtaggcg gtggaggtgg cggctgagct ggtcgcagct gccagggaaa
       61 ccgaggcggg aagcacagcg ggcagaccct caacactcgg ggtcaagtgt gcgtcccgca
      121 cgcctgaagc cgggagctcg ctccacctag gccacttctg gggtcccgca gcctccgcga
      181 gtccctgcct tgcccgaagc ccgccagggg gcgccgcgct gaggggctga ggggctgcgg
      241 ggcgggggcg ctgacggctc tgaccccggg gctgctccgg gccgggcgag aggaccgcct
      301 gggcggggac cccaaggcct cagccccgga cagaggcagg ggttcttggg gggtgcaggg
      361 ggcgcgtcgc cctctgcccc cgccggcacc ctggccatga caggcaagtc ggtgaaggac
      421 gtggatcggt accaggcggt cctggccaac ctgctgctgg aggaagataa caagttctgt
      481 gctgactgcc agtccaaagg gccgagatgg gcctcctgga acatcggggt gttcatctgc
      541 attcggtgtg ctgggatcca caggaatttg ggggtgcata tatccagggt aaaatcagtg
      601 aacctcgacc agtggactca agaacagatt cagtgcatgc aagagatggg gaatggaaaa
      661 gcaaaccgac tctatgaagc ctaccttccc gagacctttc ggcgacctca gatagaccca
      721 gctgttgaag gatttattcg agataaatat gagaagaaga aatatatgga ccgaagtctg
      781 gacatcaatg tccttaggaa agagaaggat gataagtgga aacgagggaa tgaacccgct
      841 ccggagaaaa agatggaacc tgttgttttt gagaaagtaa aaatgccaca gaaaaaagaa
      901 gacgcacagc tacctcggaa aagctctccg aaatctgcag cccctgtcat ggacttgttg
      961 ggccttgatg ctcctgtggc ctgctcaatt gcaaacagta agaccagcaa tgccctagaa
     1021 aaggatctag atcttttggc ctctgttccg tccccttctt cagtttccag aaaggctgta
     1081 ggttccatgc caactgcagg gagtgctggt tctgtccctg aaaacctgaa cctatttcca
     1141 gagccaggga gcaaatcaga agaaacaggc aagaaacagc tctccaagga ctccatccta
     1201 tcactgtatg gatcccagac gcctcaaatg cctgcccaag caatgttcat ggctcctgct
     1261 cagatggcat accccacagc ttaccccagt ttccccgggg ttacaccacc taacagcatc
     1321 atggggggca tggtgcctcc gccagtaggc atggtagctc aaccgggagc ctctggaatg
     1381 ctaaccccta tggccatgcc tgcaggctat atggggggca tgcaggcgtc catgatgggt
     1441 gtgccgaacg ggatgatgac cactcagcag gccggctaca tggcaagcat ggcagctatg
     1501 ccgcagactg tgtatggagt tcagccagct cagcaactgc aatggaacct tactcagatg
     1561 acccagcaaa tggctgggat gaacttctac ggagccaacg gcatgatgaa ctatggacag
     1621 tcaatgggcg gtggaaatgg ccaggcagcc aatcagactc tcagtcctca gatgtggaaa
     1681 taaaagcaaa gcacctgtat ggctgccact ctttttgacc cttgctttcc tccctcctct
     1741 ttttccccca gccttcccct ttcctttttc ctacctctct ctgttcggtt tagaaactga
     1801 tcataagtca tatgttgttt ggcattctgc ctaacccagt ctcccttccc agagtcatgg
     1861 acctctctgt tgctttatgt tggtcatgtc cctgactgtc cagatttcct cccaccccac
     1921 cccaccctct cccagcacca gcaccttaga aactattggc agaagagggc acatgagctg
     1981 cgggagagtg tatacactga tcagtacctc ccttgaggct gagcttcctc gcagacccac
     2041 agaaaggtct ataaaggaca agtgcttggc agaggggaaa gtagaaaggg tctttattgg
     2101 ctataagctg tttctaagtg ctgagagttt tgcttgaaga gggagcgagc catcaagttc
     2161 ccagcttttc aggaatgaaa gaagttcagc agtcagataa tccttgtgtc ttgggtcagt
     2221 ttggccctgg ccctagagtg aggcaagccc ttcctatgaa gatgagcaaa aatattattc
     2281 cacttcactc tgagtgcttt caggatctgg ggcttcaaga ctcactgctt cagtcagcct
     2341 tcattagcac caaagactcc atgaaggtcg tcccccacac aaacataccc cttgcgcacc
     2401 ttctgcctac tttgagccgg atctggcctg tgactagaag accaacccct acgaggaaat
     2461 gtggagctca acgtgtgcac tgcttgtatg cgtgtggatg tgtggatccc acctgccgcc
     2521 tccccatctg ctctgggtgt atttttgttt tttatagttc taggtttaca acagagagga
     2581 gttaatttat caacagccta aaactgttgc caggtcatct tacagtttaa aacatgccat
     2641 ctcatcggta gtccctgctc ttttccctct cccatctgcc agtctctctg tgccctcgtg
     2701 tctgagtgct gtttgcccac cttttccagg tgtaagaggc agagagcctg gacagctttc
     2761 tctctcagcc attgttcacc ccacttgaaa attcaaacaa gaaaactttg cttcaaagat
     2821 ctcatgtgtg ggaaccacag cgcctggttg cctttctcct gtgtatgtgt aaattcctta
     2881 ataaatattg cagggaaaga cccttaaaaa aaaaaaaaaa aaa
//
