LOCUS       NM_008541               6585 bp    mRNA    linear   ROD 18-MAR-2025
DEFINITION  Mus musculus SMAD family member 5 (Smad5), transcript variant 1,
            mRNA.
ACCESSION   NM_008541 XM_915786 XM_923936 XM_923937 XM_923938 XM_923940
VERSION     NM_008541.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 6585)
  AUTHORS   Lehr,S., Bruckner,D.B., Minchington,T.G., Greunz-Schindler,M.,
            Merrin,J., Hannezo,E. and Kicheva,A.
  TITLE     Self-organized pattern formation in the developing mouse neural
            tube by a temporal relay of BMP signaling
  JOURNAL   Dev Cell 60 (4), 567-580 (2025)
   PUBMED   39603235
REFERENCE   2  (bases 1 to 6585)
  AUTHORS   Allen,R.S., Biswas,S.K. and Seifert,A.W.
  TITLE     Ear pinna growth and differentiation is conserved in murids and
            requires BMP signaling for chondrocyte proliferation
  JOURNAL   Development 152 (3) (2025)
   PUBMED   39846506
REFERENCE   3  (bases 1 to 6585)
  AUTHORS   Palacio,V., Pancho,A., Morabito,A., Malkmus,J., He,Z., Soussi,G.,
            Zeller,R., Treutlein,B. and Zuniga,A.
  TITLE     Single-cell profiling of penta- and tetradactyl mouse limb buds
            identifies mesenchymal progenitors controlling digit numbers and
            identities
  JOURNAL   Nat Commun 16 (1), 1226 (2025)
   PUBMED   39890843
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6585)
  AUTHORS   Weberling,A., Siriwardena,D., Penfold,C., Christodoulou,N.,
            Boroviak,T.E. and Zernicka-Goetz,M.
  TITLE     Primitive to visceral endoderm maturation is essential for mouse
            epiblast survival beyond implantation
  JOURNAL   iScience 28 (1), 111671 (2024)
   PUBMED   39868030
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6585)
  AUTHORS   Alvarez,S., Gupta,S., Mercado-Ayon,Y., Honeychurch,K.,
            Rodriguez,C., Kawaguchi,R. and Butler,S.J.
  TITLE     Netrin1 patterns the dorsal spinal cord through modulation of Bmp
            signaling
  JOURNAL   Cell Rep 43 (11), 114954 (2024)
   PUBMED   39547237
REFERENCE   6  (bases 1 to 6585)
  AUTHORS   Chang,H., Huylebroeck,D., Verschueren,K., Guo,Q., Matzuk,M.M. and
            Zwijsen,A.
  TITLE     Smad5 knockout mice die at mid-gestation due to multiple embryonic
            and extraembryonic defects
  JOURNAL   Development 126 (8), 1631-1642 (1999)
   PUBMED   10079226
REFERENCE   7  (bases 1 to 6585)
  AUTHORS   Yang,X., Castilla,L.H., Xu,X., Li,C., Gotay,J., Weinstein,M.,
            Liu,P.P. and Deng,C.X.
  TITLE     Angiogenesis defects and mesenchymal apoptosis in mice lacking
            SMAD5
  JOURNAL   Development 126 (8), 1571-1580 (1999)
   PUBMED   10079220
REFERENCE   8  (bases 1 to 6585)
  AUTHORS   Hejlik,D.P., Kottickal,L.V., Liang,H., Fairman,J., Davis,T.,
            Janecki,T., Sexton,D., Perry,W. 3rd, Tavtigian,S.V., Teng,D.H. and
            Nagarajan,L.
  TITLE     Localization of SMAD5 and its evaluation as a candidate myeloid
            tumor suppressor
  JOURNAL   Cancer Res 57 (17), 3779-3783 (1997)
   PUBMED   9288787
REFERENCE   9  (bases 1 to 6585)
  AUTHORS   Meersseman,G., Verschueren,K., Nelles,L., Blumenstock,C., Kraft,H.,
            Wuytens,G., Remacle,J., Kozak,C.A., Tylzanowski,P., Niehrs,C. and
            Huylebroeck,D.
  TITLE     The C-terminal domain of Mad-like signal transducers is sufficient
            for biological activity in the Xenopus embryo and transcriptional
            activation
  JOURNAL   Mech Dev 61 (1-2), 127-140 (1997)
   PUBMED   9076683
REFERENCE   10 (bases 1 to 6585)
  AUTHORS   Yingling,J.M., Das,P., Savage,C., Zhang,M., Padgett,R.W. and
            Wang,X.F.
  TITLE     Mammalian dwarfins are phosphorylated in response to transforming
            growth factor beta and are implicated in control of cell growth
  JOURNAL   Proc Natl Acad Sci U S A 93 (17), 8940-8944 (1996)
   PUBMED   8799132
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            CJ087944.1, BC050001.1 and AW556382.2.
            
            On Aug 11, 2009 this sequence version replaced NM_008541.2.
            
            Transcript Variant: This variant (1) uses an alternate splice site
            in the 5' UTR, compared to variant 2. Variants 1, 2, and 3 all
            encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC050001.1, SRR1660815.279702.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-97                CJ087944.1         2-98
            98-6584             BC050001.1         1-6487
            6585-6585           AW556382.2         1-1                 c
FEATURES             Location/Qualifiers
     source          1..6585
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="13"
                     /map="13 30.12 cM"
     gene            1..6585
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /note="SMAD family member 5"
                     /db_xref="GeneID:17129"
                     /db_xref="MGI:MGI:1328787"
     exon            1..122
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /inference="alignment:Splign:2.1.0"
     exon            123..690
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /inference="alignment:Splign:2.1.0"
     CDS             288..1685
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /note="mSmad5; dwarfin-C; mothers against DPP homolog 5;
                     Smad 5; MAD homolog 5"
                     /codon_start=1
                     /product="mothers against decapentaplegic homolog 5"
                     /protein_id="NP_032567.1"
                     /db_xref="CCDS:CCDS26565.1"
                     /db_xref="GeneID:17129"
                     /db_xref="MGI:MGI:1328787"
                     /translation="MTSMASLFSFTSPAVKRLLGWKQGDEEEKWAEKAVDALVKKLKK
                     KKGAMEELEKALSSPGQPSKCVTIPRSLDGRLQVSHRKGLPHVIYCRVWRWPDLQSHH
                     ELKPLDICEFPFGSKQKEVCINPYHYKRVESPVLPPVLVPRHNEFNPQHSLLVQFRNL
                     SHNEPHMPQNATFPDSFHQPNNAPFPLSPNSPYPPSPASSTYPNSPASSGPGSPFQLP
                     ADTPPPAYMPPDDQMAPDNSQPMDTSSNMIPQTMPSISSRDVQPVAYEEPKHWCSIVY
                     YELNNRVGEAFHASSTSVLVDGFTDPSNNKSRFCLGLLSNVNRNSTIENTRRHIGKGV
                     HLYYVGGEVYAECLSDSSIFVQSRNCNFHHGFHPTTVCKIPSSCSLKIFNNQEFAQLL
                     AQSVNHGFEAVYELTKMCTIRMSFVKGWGAEYHRQDVTSTPCWIEIHLHGPLQWLDKV
                     LTQMGSPLNPISSVS"
     exon            691..942
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1062
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1284
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1541
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /inference="alignment:Splign:2.1.0"
     exon            1542..6585
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      6563..6568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /note="hexamer: AATAAA"
     polyA_site      6581
                     /gene="Smad5"
                     /gene_synonym="1110051M15Rik; Dwf-C; Madh5; MusMLP"
                     /note="major polyA site"
ORIGIN      
        1 atcatccggg tccccggcga gcgggcgccg agcgcttgtc ccggggccga gctgctaata
       61 aagttgcggc gcgtgcacag cgcggcgacg gcgtgaggag agcgcgcctg ggcggcgggg
      121 aggacttgca ctaagaagaa gcctatggca cctgtcaagt taaatgtcac tccccgcctc
      181 cacttggact ttctgcttaa gacctgcatg tgacttttca cctgcgagcc acgcttttgg
      241 tatctactga ctttgattac aggaaagtgt ctgaagattt gtatcaaatg acgtcaatgg
      301 ccagcttgtt ttctttcact agtccagccg tgaagcgatt gttgggctgg aaacaaggtg
      361 acgaggaaga gaaatgggca gaaaaggcag tggatgcttt agtgaaaaag ctgaagaaga
      421 agaagggtgc tatggaggag ctggagaaag ccttgagcag cccaggacag ccaagcaagt
      481 gtgtcacgat ccccaggtcc ttggatggac gtctgcaagt ttctcacagg aaaggcttgc
      541 cccatgttat atattgccgt gtttggcgct ggccagattt gcagagccat cacgagctaa
      601 aaccattgga tatttgtgaa tttccttttg gatctaagca aaaggaagtt tgtatcaatc
      661 cataccacta taagagagtg gagagtccag tcttacctcc agtattagtg cctcgtcaca
      721 atgaattcaa tccacaacac agccttctgg ttcagttcag gaacctgagc cacaatgaac
      781 cgcacatgcc acaaaacgcc acgtttcccg attctttcca ccaacccaac aacgctcctt
      841 tccccttatc tcctaacagc ccctatcctc cttcccctgc tagcagcaca tatcccaact
      901 ccccagcaag ctctggacct ggaagtccat ttcaactccc agctgacacc cctccccctg
      961 cctatatgcc acctgatgat cagatggccc cagataattc ccagcctatg gatacaagca
     1021 gtaacatgat tcctcagacc atgcccagca tatccagcag agatgttcag cctgtcgcct
     1081 atgaggagcc caaacactgg tgttcgattg tctactatga attaaacaat cgtgttgggg
     1141 aagcttttca tgcatcttct actagtgtgt tagtagatgg atttacagat ccttcaaata
     1201 acaaaagtag attctgcctg ggattgttgt caaatgttaa tcgtaattca actattgaaa
     1261 acactaggcg gcatattgga aaaggtgttc atctatacta cgttggtggg gaggtgtacg
     1321 ctgagtgtct tagtgacagc agcatctttg ttcagagtag gaactgcaac tttcaccatg
     1381 gcttccatcc caccaccgtc tgtaagatcc ccagcagctg cagcctcaag atttttaaca
     1441 atcaggagtt tgctcagctt ctggctcagt cagtcaacca tggattcgag gctgtgtatg
     1501 agctcaccaa gatgtgtacc attcgaatga gctttgtcaa gggctgggga gcagagtacc
     1561 accgacagga cgtcaccagt actccctgct ggattgagat tcacctccac gggcctctgc
     1621 agtggctgga taaagtcctt actcagatgg gctctccgct gaaccccatt tcttctgttt
     1681 catagtgcag aagtattctt tcaactatat ttttagtgga cttgttttaa ttttagagga
     1741 atttccagta cagatgctgt gagctgacat ggaaaacaga tattattttt tctacgtaat
     1801 tgtgaccaac acatttgtat tttatgatga tattacattt gtttgtattc gtgttcattg
     1861 tgattaactt tcaaaagtat tgtaaacgat gtagagtatt ttgcccctgt tgaaatgttt
     1921 agcattgatc ttaaactgga acgtactttt tcttattgtc ccaacgtttt ttaatttgtt
     1981 aaattttttt tacaaagtag ttcatcacat aatgaaattt tatcctataa gagaacatat
     2041 attgtggaaa gcagtagatg atatttctct gggaatttct ttgccttacc acctttgaaa
     2101 aagcatacat tgtttgcaaa acctcaaagt agggcttgct taaaggaaac tgttgaatct
     2161 tgtttgaagg acactgcagt cctaacgtgt tcagtgaaag caaggtggta gatttctgga
     2221 cgtcatacat ttacatttaa tataggtaat attcatcagt gtaatgtgac ttcatgccat
     2281 atatattttg taaaacaatt cctttttaaa aacttcaagt atttctcatt tactcaaatt
     2341 tgttgtaagt cctacttaac agttagttac tatgtgctct gtggccttgt tcagcattgt
     2401 ttgctgcttt gggccaacaa ttcaagaact ctaattttcc tgtgcattaa tcttttcatt
     2461 ttgcactttt atgggtgact gtcttagtgt agcctctggt aaaatactat taggtggcct
     2521 ggttttagag ctcctcctcg ctgccttggc actcctttgt gcaacacgac cacttagaga
     2581 tgacagctgt gagctgtgct gctttttcta gcctttaatt tccaatgtag tttataatgt
     2641 tgttcttcta tagctccagc taaggtgcct gttagtcccc tacaatgtta tgagcattat
     2701 tgacattgaa aggttatgta tgtatgaata cctttgctcc ttaccagact tgtcatacaa
     2761 ggactcgtgc agtgtagcca gtagaggctc tttggttggc ccaagaatga ggctgttggt
     2821 gtaagtgaat cacaataggg attgggatag ttcatgtcat atgtcatata gcaagacaat
     2881 gtagagtgta ggcttgtctc tctgcatcaa cgctctgcct ctttcttttt atccttttag
     2941 aacctacatg gacgctaatc tccacaacac tgttggatgt gaacactctt aagacactca
     3001 tctagttcac tgtgccttgt ccttaggact cttaaccact ttctagggag cagttatggc
     3061 ctgagatgga cagtcatggc ctgagaatga agacactact ttgataaaga aaaaggcctc
     3121 atttgcctat cagagtgaga aaggtttttt tctggtgcct tttgaaaata tacagagcca
     3181 cttggttctt ctgctgaaaa tgtaattttg gtttgacttt ttagagtgcc cttcctgcct
     3241 ttatgaggaa aacagctatt tttttttttg ggggggggga ttccttttgt tttctgtgcc
     3301 attatttatt gcctttcaga gtgcaaccat tgggtggctt tgctccttca gagagggctc
     3361 cttgatagcc ttcagtagct tgagctgtag acataagtat tccatagcaa gagtgtgtca
     3421 gctccatgag agagatgtct gctttatagc cgaggcagaa accgttcatg ttcctttact
     3481 tggcagcctt caggaacagg tttgtaagaa cgtgtcttga gttgagtgag tgtatgtctg
     3541 tgagctctgc tgaagtctgg acacaagggc cttgcctgct ccttttttca gcagtgggtt
     3601 acatgttgtc tctccacagt cttcatgtca taggtctcgg acttgcagag tcctatgtgg
     3661 cctgccatct gtacagtggc aggactgaag ctctgagctg ttctgaggtt catggagaaa
     3721 tcccaaccta ttctgtggtc agtaaatgga gactgtgtag tctacctgct cctgtactgt
     3781 ccttactgta tgtaaggata tacagacgcc tgtgggtagg cagtactcac agtgagatga
     3841 agacagcaag tgtgcactga accacagagg gcagggagta gggcctctga agaagccacc
     3901 agaccagacc agtgccggta cagtctttgt cagagatggc tctgatgggg cccagactga
     3961 ccctgaccat gctgagttgc tgagggtagc cttcagttct ctaccctctg aagtgctagg
     4021 atgacagaca tccgccatca tacccagctt ccgtggtgct aaggatcagc ctcagtcttc
     4081 aggcgtgcta ggcatgtact ttgccaagta tttagtatac aaaatacatt agtatctgcc
     4141 agggaaaaaa gatttgcaaa taataaagat tgccatcagt ttgataaatg ttgtaaatgg
     4201 aagaatcaaa atctcagcga tggattacag caacaagatg ctgcctagga aaagcaggac
     4261 caagaggtac atttgactag tataccttca gcgtagcgtg atgacctcac tgatgtcacc
     4321 caactgaact taagggctgt aagtaggcgt gctgtgggcc ttccagaact agagaaaatt
     4381 ataggaggaa gtcagttcta aagtatcaaa agctgggtaa tggtggcaca tgcctttgat
     4441 tctagcactc gggaagcagg ggctagccta gtctacagag caacttctac acagagaaac
     4501 tgtcttggag gaaaataaaa aaagaaaagt caaagagcaa acaaatagaa cagagtagga
     4561 atccgtgtcc ccttttttct atgtttcacg gttgcaggtg taagaaaagt agtcatagat
     4621 gtggctgagt ttctaagatg aaaccagtag taagattgct aaatataaca cttcaaccaa
     4681 gttaaacacc ctttgggggt atgaatgaaa gtaacactgc aatatgaaat gaaccgtgca
     4741 agtaacactt ggggttacct cacagtctcc ctatgcctga gaggactgtg ggaaacattt
     4801 ccatcccctg ccagtatcgc cattgggagg acagagtaga tgaagaagtg aagtcttact
     4861 ggtccagggc acgcctgtca gcaatgccat ttgtgcttct gccacagaga gcaccgagag
     4921 gcttggctca gtatcctcga accttctctg gtcacttccc tggcagcact tgggtccctg
     4981 tcactcactg gtctcttaaa agtcccgtct ctttgcttcc taaagattct ctaaaaaaat
     5041 tactattttt tatttctttt ttaaaagtct ttgttatttt gttttgggat acagtctctt
     5101 tgtacagtcc tggctggcct ggaaattact atgtaggcca gcctcaaact tgaagtaatt
     5161 ctcttgcctc tgcctctgga gttctgggat tacaggcatg cactgcagag tacagtgagc
     5221 tctgatggct tttaaaattc agcccctttg agggtttggt tttagatcca ttagctttgt
     5281 ctgaacccat ctttgtccgg ccgagtaaat cctctgctat ccggggtctc ggtagaaatg
     5341 tgttctcagt atacatacga ctaaacattg gttgtttata ggtagcctca gatatttggt
     5401 agagcatctt ttttgaaagt aatctccagc taggtgggta tttccctcac agcagtagga
     5461 ttttcccttt aggagatacc agttcttcat ctttcttgtg aaaataatgc ctttatgggg
     5521 agtgaagatt aaggagttgt ttctacacta acagaattct atttgatgga caacttggac
     5581 agttctgtgg acttgggtgg gttctagtgt gctaagaagg ataacagtat ttaatagtgt
     5641 ctgtcatcag gccttgctca tctccctgtc tagggctgta ggtcagtgct cgagcactta
     5701 gcaggcatcg agtctagtgt tcagtgccca gcattgcaca gaactcagaa tatatctgta
     5761 ctgaaactga agtgaccacc tacaaccagg tggtatgcca gaaccacaga aaggagattc
     5821 acggtgatgt gtttaaagca ttgggctggt gacggttgct gtgtagtaat gacctcttcc
     5881 tcagcaaaga gagtcctgga gcaggctgtc ctcagaagag ggaagggact ggtgtgctcc
     5941 ttgtgcagat aacttagtgt ataaatcggc atgagtagct atcctttaag gatttgtttg
     6001 aagttactct ttgtaaaaag ttgagaattt tgtgtgcagt tgggcacatg cttgcccttc
     6061 ccccacccgc catagtcctg cctctcttgc tgtgaactgg tgtcagctac aacactccag
     6121 ctaggtctga gctcttttga gagaaggtct cgtagagcac cattctcaga gagaagctaa
     6181 agcatgggga gccttaggac ggtcaggcaa tgcactcttt accacggctg gctaaggctg
     6241 cagcttgacc gtccttacct aaatcaggta agaatgtgat tacagagcga gtgcttgtgt
     6301 tccccggcct gccttctccg aggaagatgc ttcatccgag gatgatgcag agcagacgat
     6361 ggctgcactg taggtctgcc tccttctgtg tatgggttct gctgctgctt acggcatagg
     6421 aaagtacact agcagcgtgc ttcaattctg ccatcttttg atacttataa aaatgtatta
     6481 ggttttactg tattgtgctc tcaaagccat aactcttaag aaatttggtt tttttgcata
     6541 ttgtttgcta atactttgtt ttaataaacc tcaaaatctg cttac
//
