LOCUS       NM_178804               9958 bp    mRNA    linear   ROD 24-DEC-2024
DEFINITION  Mus musculus slit guidance ligand 2 (Slit2), transcript variant 3,
            mRNA.
ACCESSION   NM_178804
VERSION     NM_178804.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 9958)
  AUTHORS   Delpech,C., Schaeffer,J., Vilallongue,N., Delaunay,A., Benadjal,A.,
            Blot,B., Excoffier,B., Plissonnier,E., Gascon,E., Albert,F.,
            Paccard,A., Saintpierre,A., Gasnier,C., Zagar,Y., Castellani,V.,
            Belin,S., Chedotal,A. and Nawabi,H.
  TITLE     Axon guidance during mouse central nervous system regeneration is
            required for specific brain innervation
  JOURNAL   Dev Cell 59 (24), 3213-3228 (2024)
   PUBMED   39353435
REFERENCE   2  (bases 1 to 9958)
  AUTHORS   Gomez-Del Arco,P., Isern,J., Jimenez-Carretero,D.,
            Lopez-Maderuelo,D., Pineiro-Sabaris,R., El Abdellaoui-Soussi,F.,
            Torroja,C., Vera-Pedrosa,M.L., Grima-Terren,M., Benguria,A.,
            Simon-Chica,A., Queiro-Palou,A., Dopazo,A., Sanchez-Cabo,F.,
            Jalife,J., de la Pompa,J.L., Filgueiras-Rama,D., Munoz-Canoves,P.
            and Redondo,J.M.
  TITLE     The G4 resolvase Dhx36 modulates cardiomyocyte differentiation and
            ventricular conduction system development
  JOURNAL   Nat Commun 15 (1), 8602 (2024)
   PUBMED   39366945
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 9958)
  AUTHORS   Curran,B.M., Nickerson,K.R., Yung,A.R., Goodrich,L.V., Jaworski,A.,
            Tessier-Lavigne,M. and Ma,L.
  TITLE     Multiple guidance mechanisms control axon growth to generate
            precise T-shaped bifurcation during dorsal funiculus development in
            the spinal cord
  JOURNAL   Elife 13, 94109 (2024)
   PUBMED   39159057
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9958)
  AUTHORS   Rama,N., Dubrac,A., Mathivet,T., Ni Charthaigh,R.A., Genet,G.,
            Cristofaro,B., Pibouin-Fragner,L., Ma,L., Eichmann,A. and
            Chedotal,A.
  TITLE     Slit2 signaling through Robo1 and Robo2 is required for retinal
            neovascularization
  JOURNAL   Nat Med 21 (5), 483-491 (2015)
   PUBMED   25894826
  REMARK    GeneRIF: Slit2 signaling through Robo1 and Robo2 has a role in
            retinal neovascularization
REFERENCE   5  (bases 1 to 9958)
  AUTHORS   Grieshammer,U., Le,Ma., Plump,A.S., Wang,F., Tessier-Lavigne,M. and
            Martin,G.R.
  TITLE     SLIT2-mediated ROBO2 signaling restricts kidney induction to a
            single site
  JOURNAL   Dev Cell 6 (5), 709-717 (2004)
   PUBMED   15130495
  REMARK    GeneRIF: Mutants lacking SLIT2 develop supernumerary ureteric buds
            that remain inappropriately connected to the nephric duct; the
            SLIT2 signal is transduced in the nephrogenic mesenchyme.
REFERENCE   6  (bases 1 to 9958)
  AUTHORS   Bagri,A., Marin,O., Plump,A.S., Mak,J., Pleasure,S.J.,
            Rubenstein,J.L. and Tessier-Lavigne,M.
  TITLE     Slit proteins prevent midline crossing and determine the
            dorsoventral position of major axonal pathways in the mammalian
            forebrain
  JOURNAL   Neuron 33 (2), 233-248 (2002)
   PUBMED   11804571
REFERENCE   7  (bases 1 to 9958)
  AUTHORS   Plump,A.S., Erskine,L., Sabatier,C., Brose,K., Epstein,C.J.,
            Goodman,C.S., Mason,C.A. and Tessier-Lavigne,M.
  TITLE     Slit1 and Slit2 cooperate to prevent premature midline crossing of
            retinal axons in the mouse visual system
  JOURNAL   Neuron 33 (2), 219-232 (2002)
   PUBMED   11804570
REFERENCE   8  (bases 1 to 9958)
  AUTHORS   Holmes,G.P., Negus,K., Burridge,L., Raman,S., Algar,E., Yamada,T.
            and Little,M.H.
  TITLE     Distinct but overlapping expression patterns of two vertebrate slit
            homologs implies functional roles in CNS development and
            organogenesis
  JOURNAL   Mech Dev 79 (1-2), 57-72 (1998)
   PUBMED   10349621
REFERENCE   9  (bases 1 to 9958)
  AUTHORS   Hassoun,H., Hanada,T., Lutchman,M., Sahr,K.E., Palek,J., Hanspal,M.
            and Chishti,A.H.
  TITLE     Complete deficiency of glycophorin A in red blood cells from mice
            with targeted inactivation of the band 3 (AE1) gene
  JOURNAL   Blood 91 (6), 2146-2151 (1998)
   PUBMED   9490702
REFERENCE   10 (bases 1 to 9958)
  AUTHORS   Maekawa,T.L., Takahashi,T.A., Fujihara,M., Urushibara,N.,
            Kadowaki-Kikuchi,E., Nishikawa,M., Ikebuchi,K., Asano,S., Ozawa,K.
            and Sekiguchi,S.
  TITLE     A novel gene (drad-1) expressed in hematopoiesis-supporting stromal
            cell lines, ST2, PA6 and A54 preadipocytes: use of mRNA
            differential display
  JOURNAL   Stem Cells 15 (5), 334-339 (1997)
   PUBMED   9323794
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK053182.1, BC079911.1,
            AK033292.1, AC101004.6, AW556809.1 and BE944330.1.
            
            On Sep 11, 2015 this sequence version replaced NM_178804.4.
            
            Summary: The protein encoded by this gene is a member of the Slit
            family of secreted glycoproteins, which function as ligands for the
            Robo family of immunoglobulin receptors. Slit proteins play highly
            conserved roles in axon guidance and neuronal migration and may
            also have functions during other cell migration processes including
            leukocyte migration. In mammals, members of the slit family are
            characterized by an N-terminal signal peptide, four leucine-rich
            repeats, nine epidermal growth factor repeats, and a C-terminal
            cysteine knot. Mice deficient for this gene exhibit abnormal axonal
            projections in the embryonic forebrain and develop supernumerary
            uretic buds that maintain improper connections to the nephric duct.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Sep 2015].
            
            Transcript Variant: This variant (3) lacks three alternate in-frame
            exons compared to variant 1. The encoded isoform (c) is shorter
            than isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC079911.1, AF144628.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849381, SAMN00849385
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1875              AK053182.1         2-1876
            1876-3401           BC079911.1         1-1526
            3402-3474           AK033292.1         892-964
            3475-7721           BC079911.1         1600-5846
            7722-9293           AC101004.6         141482-143053       c
            9294-9577           AW556809.1         367-650             c
            9578-9958           BE944330.1         1-381               c
FEATURES             Location/Qualifiers
     source          1..9958
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 26.05 cM"
     gene            1..9958
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /note="slit guidance ligand 2"
                     /db_xref="GeneID:20563"
                     /db_xref="MGI:MGI:1315205"
     exon            1..2064
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     CDS             1886..6451
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /note="isoform c precursor is encoded by transcript
                     variant 3; slit homolog 2 protein; neurogenic
                     extracellular slit protein; downregulated during adipocyte
                     differentiation-1"
                     /codon_start=1
                     /product="slit homolog 2 protein isoform c precursor"
                     /protein_id="NP_848919.3"
                     /db_xref="CCDS:CCDS19280.1"
                     /db_xref="GeneID:20563"
                     /db_xref="MGI:MGI:1315205"
                     /translation="MSGIGWQTLSLSLGLVLSILNKVAPQACPAQCSCSGSTVDCHGL
                     ALRSVPRNIPRNTERLDLNGNNITRITKIDFAGLRHLRVLQLMENRISTIERGAFQDL
                     KELERLRLNRNNLQLFPELLFLGTAKLYRLDLSENQIQAIPRKAFRGAVDIKNLQLDY
                     NQISCIEDGAFRALRDLEVLTLNNNNITRLSVASFNHMPKLRTFRLHSNNLYCDCHLA
                     WLSDWLRQRPRVGLYTQCMGPSHLRGHNVAEVQKREFVCSGHQSFMAPSCSVLHCPAA
                     CTCSNNIVDCRGKGLTEIPTNLPETITEIRLEQNSIRVIPPGAFSPYKKLRRLDLSNN
                     QISELAPDAFQGLRSLNSLVLYGNKITELPKSLFEGLFSLQLLLLNANKINCLRVDAF
                     QDLHNLNLLSLYDNKLQTVAKGTFSALRAIQTMHLAQNPFICDCHLKWLADYLHTNPI
                     ETSGARCTSPRRLANKRIGQIKSKKFRCSGTEDYRSKLSGDCFADLACPEKCRCEGTT
                     VDCSNQRLNKIPDHIPQYTAELRLNNNEFTVLEATGIFKKLPQLRKINFSNNKITDIE
                     EGAFEGASGVNEILLTSNRLENVQHKMFKGLESLKTLMLRSNRISCVGNDSFIGLGSV
                     RLLSLYDNQITTVAPGAFDSLHSLSTLNLLANPFNCNCHLAWLGEWLRRKRIVTGNPR
                     CQKPYFLKEIPIQDVAIQDFTCDDGNDDNSCSPLSRCPSECTCLDTVVRCSNKGLKVL
                     PKGIPKDVTELYLDGNQFTLVPKELSNYKHLTLIDLSNNRISTLSNQSFSNMTQLLTL
                     ILSYNRLRCIPPRTFDGLKSLRLLSLHGNDISVVPEGAFNDLSALSHLAIGANPLYCD
                     CNMQWLSDWVKSEYKEPGIARCAGPGEMADKLLLTTPSKKFTCQGPVDITIQAKCNPC
                     LSNPCKNDGTCNNDPVDFYRCTCPYGFKGQDCDVPIHACISNPCKHGGTCHLKEGENA
                     GFWCTCADGFEGENCEVNIDDCEDNDCENNSTCVDGINNYTCLCPPEYTGELCEEKLD
                     FCAQDLNPCQHDSKCILTPKGFKCDCTPGYIGEHCDIDFDDCQDNKCKNGAHCTDAVN
                     GYTCVCPEGYSGLFCEFSPPMVLPRTSPCDNFDCQNGAQCIIRINEPICQCLPGYLGE
                     KCEKLVSVNFVNKESYLQIPSAKVRPQTNITLQIATDEDSGILLYKGDKDHIAVELYR
                     GRVRASYDTGSHPASAIYSVETINDGNFHIVELLTLDSSLSLSVDGGSPKVITNLSKQ
                     STLNFDSPLYVGGMPGKNNVASLRQAPGQNGTSFHGCIRNLYINSELQDFRKMPMQTG
                     ILPGCEPCHKKVCAHGMCQPSSQSGFTCECEEGWMGPLCDQRTNDPCLGNKCVHGTCL
                     PINAFSYSCKCLEGHGGVLCDEEEDLFNPCQMIKCKHGKCRLSGVGQPYCECNSGFTG
                     DSCDREISCRGERIRDYYQKQQGYAACQTTKKVSRLECRGGCAGGQCCGPLRSKRRKY
                     SFECTDGSSFVDEVEKVVKCGCARCAS"
     exon            2065..2136
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..2208
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2209..2280
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2281..2352
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2424
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2425..2496
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2497..2660
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2661..2799
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2800..2871
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2872..2943
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            2944..3015
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3016..3159
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3160..3323
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3324..3474
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3475..3549
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3550..3693
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3694..3837
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            3838..4004
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4005..4137
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4138..4206
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4207..4278
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4279..4350
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4351..4422
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4423..4586
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4587..4711
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4712..4809
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4810..4949
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            4950..5043
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5044..5181
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5182..5422
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5423..5553
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5554..5708
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5709..5997
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            5998..6209
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     exon            6210..9945
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7699..7704
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /note="hexamer: AATAAA"
     polyA_site      7721
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /note="major polyA site"
     regulatory      9911..9916
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
                     /note="hexamer: AATAAA"
     polyA_site      9945
                     /gene="Slit2"
                     /gene_synonym="b2b1200.1Clo; Drad-1; E030015M03Rik;
                     E130320P19Rik; mKIAA4141; Slil3; slit-2"
ORIGIN      
        1 ggacttgggc ttatttattt acggagcgcc tggacggcgg aggctggagg aggcggcggc
       61 tgtggctgcc gcagacggtg gtttaaaaaa aggaggagaa ggaggcggcg gcggcggcga
      121 gggatgggag cgcccaggct tcagggcgca ggccggagct gccgagcttt cctgaggctg
      181 cgggagtact gaggagaaaa aggggacgtc ccgggggccc acaggcgact ccagaggcgc
      241 ggactggggt ctttttgttt ggctgcgctc agcgcccagc agccagagcg gacaactcca
      301 gttacaacaa caacccacct tccagcagcc aggcgagact taggctgcgg agtccgtcct
      361 attgtttaga cacttgcctg gggctctgga gcgccagtcc ctgctcagtc tccgccttcc
      421 ctctctctcc actttcctcc cgggaaccgc gagtgccgaa agcctccctg agaggggaag
      481 gggctggaag ccaagcgctg cctagtctgt ggtcgccgag tcaggtgcaa cgtccggggc
      541 taagcggcat ctcaacgtct gcggctggac tcctcccgtg acagtgggtg accggatcca
      601 acgcctgggg aaacttcctg cactttgagg ctgaggaccc acttgtaggg gctgcccgag
      661 gggtggctga gaggctgcat acagcaggac gtcgctgttg gctgttgttt cttctacctg
      721 tgccgtcagg tgtgtgctgc ggagctgcgg cgtatcccgg agaccagagt ggctgacaag
      781 agcgcgcaca ctctactgcc attcggttgt cgcctggggt ctgccagggt agcagatccc
      841 catctttctg ccttctacct agtagtcatc catcagggca cagtagcagc actggcatcg
      901 cggacaccta gtctttgctt tgctcttctg ggctcgccta tctgcaaatc ttgcccaagc
      961 tagaggacct ttcctactca acttggctta ctggattttt ccccactgca ggagtgagga
     1021 ctagtggata ttcctgcaag gctgcagcag tccgactgcc cttttgtctc tctgtgtgac
     1081 ctcaggggca ggccctggtg cagagcgtcg ccaaggacgc cgggagggag gcgggattgc
     1141 caagatatcc tccagtgaag tgcatgtgtg tgtgcaagcc atccttggct gtcgggaagt
     1201 ggagaagacg gttgaggctg ctgctgtgcc gcaggagtgg agaggccccg ggagctgagc
     1261 cctgagcccc gcatcaccgc ccggcacccc caaggctgtc tgaatacccg gtgcgccccc
     1321 acgcgcgcca tgaccagtct ctccgagggc tctgctttgg acctgccagg cccttgcgcc
     1381 ttctagcttc gggggaatcc actttgatca gggccaccat tactgtttaa gttccctcct
     1441 cagccttgta ctctttccac tggaatcgga tttgctagag ggttcgtggg atcagaagtc
     1501 ctcccttgtc ctcaagcaac cagcctctgc atcttcgcgg acactgcaag taggagctct
     1561 tttaccgcca agttgaagtc gcgcactgtc ctcacagctg ctccggggtc tactcccagc
     1621 ctgagtcggg cctattgata ttcagaactt gaagttgccc acggatcttc tgctctgcta
     1681 gaaaggcttg aagagcagag gaaagacctg tgcttctgtc agctctcctg ccccatatca
     1741 ctgttccaga ttactgtgtg agcatctccc cgggtgctgt gggctgcaag cccagcgcca
     1801 ggcactgggc ctcggacact gcccggtttt tacacaaccg aaagctcaag agaagtcctt
     1861 caaagcaagg agtctattag ggaagatgag tggcattggc tggcagacac tgtccctatc
     1921 gctggggtta gtgttgtcga tcttgaacaa ggtggcgccg caggcgtgcc cggcccagtg
     1981 ctcctgttca ggcagcacgg tggactgtca tgggctggca ctgcgcagtg tgcccaggaa
     2041 tatcccccgc aacaccgaga gactggattt gaatggaaat aacatcacga ggatcacgaa
     2101 gatagatttt gctggtctca ggcacctcag agttcttcag ctcatggaga acagaatcag
     2161 caccatcgag aggggagcat tccaggatct taaggagctg gaaagactgc gtttaaacag
     2221 aaataacctt cagttgtttc ctgagctgct gtttctcggg actgcgaagc tctaccggct
     2281 tgatctcagt gaaaatcaaa ttcaagcaat tccaaggaag gctttccgtg gggcagttga
     2341 cattaaaaac ctgcaactgg attacaacca gatcagctgc attgaagatg gggcgttcag
     2401 agctctacga gatctggaag tgctcactct gaacaataac aatattacta gactttcagt
     2461 ggcaagtttc aaccatatgc ctaaacttag gacatttcga ctccactcga acaacttgta
     2521 ctgcgactgc cacctagcct ggctctcaga ctggcttcgc caaaggccac gggtgggctt
     2581 gtacactcag tgtatgggcc catcccacct gaggggccac aatgtagcag aggttcaaaa
     2641 acgagagttt gtctgcagtg gtcaccagtc attcatggct ccctcctgca gtgtgctgca
     2701 ctgccccgct gcttgtacct gtagcaacaa cattgtagac tgccgaggga aaggtctcac
     2761 tgagatcccc acaaatctgc ctgagaccat cacagaaata cgtttggaac agaactccat
     2821 cagggtcatc cctccaggag ccttctcacc atacaaaaag cttagacgac tagacctgag
     2881 caacaaccag atctctgaac ttgcaccaga tgccttccaa ggactgcgct ctctgaattc
     2941 acttgtcctg tatggaaata aaatcacaga actcccaaaa agtttattcg aaggactatt
     3001 ttccttgcag ctactattat tgaatgccaa caagataaac tgccttcggg tagatgcttt
     3061 tcaggacctg cacaacttga accttctctc cttatatgac aataagcttc agacggttgc
     3121 caagggcacc ttctcagccc tcagagccat ccaaactatg catttggccc agaatccttt
     3181 catttgtgac tgccatctca agtggctagc ggattatctc cacaccaacc caattgagac
     3241 cagcggtgcc cgttgcacca gcccccgccg cctggcaaac aaaagaattg gacagatcaa
     3301 aagcaagaaa ttccgttgtt caggtacaga agattatcga tcaaaattaa gtggagactg
     3361 ctttgcagac ttggcttgtc ctgagaagtg tcgctgtgaa gggaccacag tagactgctc
     3421 caatcaaaga ctcaacaaaa tccctgacca tattccccag tacacagcag agctgcgtct
     3481 caataataat gaattcacag tgttagaagc cacgggaata tttaagaaac ttcctcagtt
     3541 acgtaaaatc aactttagca acaataagat cacggatatc gaggagggtg catttgaagg
     3601 cgcgtctggt gtgaatgaaa ttcttctcac cagtaaccgt ttggaaaatg ttcagcataa
     3661 gatgttcaaa ggactggaga gcctcaaaac attgatgctg agaagtaatc gaataagctg
     3721 tgttgggaac gacagtttca taggactcgg ctctgtgcgt ctgctctctt tatatgacaa
     3781 tcaaattacc acagtggcac caggagcatt tgattctctc cattcattat ccactctaaa
     3841 cctcttggcc aatcctttca actgtaactg tcacctggca tggctgggag aatggctcag
     3901 aaggaaaaga attgtaacag gaaatcctcg atgccaaaaa ccctacttcc tgaaggaaat
     3961 cccaatccag gatgtagcca ttcaggactt cacctgtgat gatggaaatg atgacaatag
     4021 ttgctctcca ctctcccgtt gtccttctga atgtacctgc ttggatacag tggtacgatg
     4081 tagcaacaag ggcttgaagg ttttgcctaa aggtattcca aaagatgtca cagagctgta
     4141 tctggatggg aaccagttta cgctggtccc gaaggaactc tctaactaca aacatttaac
     4201 acttatagac ttaagtaaca accgaataag caccctttcc aatcaaagct tcagcaacat
     4261 gacccagctt ctcaccttaa tcctcagtta caaccgtctg agatgtatcc ctccacgaac
     4321 ctttgatgga ttgaagtctc ttcggttact gtctttacat ggaaatgaca tttctgttgt
     4381 gcctgaaggt gccttcaatg acttgtcagc cttgtcacac ttagcgattg gagccaaccc
     4441 tctttactgt gattgtaaca tgcagtggtt atccgactgg gtgaagtcgg aatataagga
     4501 acctggaatt gcacgctgtg ccggccctgg agaaatggca gataaattat tactcactac
     4561 tccctccaaa aaatttacat gtcaaggtcc cgtggatatc actattcaag ccaagtgtaa
     4621 tccctgctta tcaaatccat gtaaaaatga tggcacctgt aacaatgacc ccgttgattt
     4681 ttatcgatgt acctgcccat atggattcaa gggtcaggac tgtgatgtcc ccattcatgc
     4741 ttgtatcagt aatccatgta aacatggagg aacttgtcac ttaaaggaag gagagaatgc
     4801 tggattctgg tgcacttgtg ctgatgggtt tgaaggagaa aactgtgaag tcaatattga
     4861 tgattgtgaa gataatgatt gtgaaaataa ttctacatgc gttgatggaa ttaacaacta
     4921 cacatgtctt tgcccaccgg aatacacagg tgaactgtgt gaggaaaagc tggacttctg
     4981 tgcacaagac ttgaatccct gccagcatga ctccaagtgc atcctgactc caaagggatt
     5041 caagtgtgac tgcactccag gatacattgg tgagcactgt gacattgact ttgatgactg
     5101 ccaagataac aagtgtaaaa acggtgctca ctgcacagat gccgtgaacg gatacacgtg
     5161 cgtctgtcct gaaggctaca gtggcttgtt ctgtgagttt tctccaccca tggtcctccc
     5221 tcgcaccagc ccctgtgata attttgattg ccagaatgga gcccagtgta tcatcaggat
     5281 aaatgaacca atatgccagt gtttgcctgg ctacctggga gagaagtgtg agaaattggt
     5341 cagtgtgaat tttgtaaaca aagagtccta tcttcagatt ccttcagcca aggttcggcc
     5401 tcagacaaac atcacacttc agattgccac agatgaagac agcggcatcc tcttgtataa
     5461 aggtgacaaa gaccacattg ccgtggaact ctatagaggg cgagttcgag ccagctatga
     5521 caccggctct catccggctt ctgccattta cagtgtggag acaatcaatg atggaaactt
     5581 ccacattgtg gagctactga ccctggattc cagtctttcc ctctctgtgg atggaggaag
     5641 ccctaaagtc atcaccaatt tgtcaaaaca atctactctg aatttcgact ctccactcta
     5701 tgtaggaggc atgcctggga aaaataacgt ggcatccctg cgccaggccc ctgggcaaaa
     5761 tggcaccagc ttccatggct gtatccggaa cctttacatt aacagtgagc tgcaggactt
     5821 ccggaaaatg cctatgcaaa ccggaattct gcctggctgt gaaccatgcc acaagaaagt
     5881 atgtgcccat ggcatgtgcc agcccagcag ccaatcaggc ttcacctgtg aatgtgagga
     5941 agggtggatg gggcccctct gtgaccagag aaccaatgat ccctgcctcg gaaacaaatg
     6001 tgtgcatggg acctgcctgc ccatcaatgc cttctcctat agttgcaagt gcctggaggg
     6061 ccatggcggt gtcctctgtg atgaagaaga agatctcttt aacccctgcc agatgatcaa
     6121 gtgcaagcat gggaagtgca ggctttctgg agtgggccag ccctattgtg aatgcaacag
     6181 tggattcacc ggggacagct gtgatagaga aatttcttgt cgaggggaac ggataaggga
     6241 ctattaccag aagcagcagg gttacgctgc ctgtcaaaca actaagaaag tatctcgctt
     6301 ggaatgcaga ggcgggtgcg ctggaggcca gtgctgtgga cctctgagaa gcaagaggcg
     6361 gaaatactct ttcgaatgca cagatggctc ctcatttgtg gacgaggttg agaaagtggt
     6421 gaagtgcggc tgcgcgagat gtgcctccta agcgcgtctc tagaagcttc tagcttcggc
     6481 gaaggttgta cacttcttga ccatgttgga ctaattcatg cttcataatg gaaatatttg
     6541 aaatatattg taaaatacag aacagactta tttttattat gataataaag acttgtctgc
     6601 atttggaaaa aaataataat aaaagccacg cttgtactaa agctccccct acactggaga
     6661 agtgtggaga aaagccacac ttggaggcat tagtgaagcg gtgggtacca ttgcaacacg
     6721 gagccatctt tggaacacgc caatactagc agaagcacat ctacaagagc ctgacatggg
     6781 actgtacgct catatgcgct tgcctagagc gtcaaacccg tgaccccctt cacatcagtt
     6841 cccaaggaca catcgagcac aagtgtgtga gtgagactga gaggtagatg aatgggaatc
     6901 tagaatctgt agataggtcc aagggatgag atatattttg tgcaagatct aaagtgtcct
     6961 gacatctggg tttcattccg aaagaaatgg gtgtggagtg ctgtgatgta ttttattgtc
     7021 tttggcaatg acagcgcata ggcagaaaca gcacacgaag gtgtttagct accagtttcc
     7081 agtattaaat tttttgtaat ataaatgaca aaggagatga taaagaacca atagattatt
     7141 gataaataaa aattagtaat aatatgaatt tttgtttcta tgagttctaa atatccctat
     7201 acagtattca ttttcaatga ggaatattta ttgtattaaa gtacatttta cttaacgatt
     7261 tagatcatca ttttggactg tttcttgatg ctttaatata tattaattta taattatcct
     7321 gatatttttg tacattttca aactttaaaa aaaaatcagg atttttttgg caatagtact
     7381 aacataggat gttattttgt tatctcggga taagtatatg ttgttctgtt ggctgacctt
     7441 gatgtctgac cattgatgcc actgaactgt ggcctccttc aggacacttg cagaaggttt
     7501 gcaaagtctc agagtagaaa cagcaagtga atctatctgc cacatgtcct cacaagaaga
     7561 aagaacttat ctgtagcaat ggccagtaag aagtagatgt tttacaaatt aattaataaa
     7621 catttccctg ccatgctttg ttggggaggg tgggagaaga aagactgtta tgtcaaaaat
     7681 tgtaatttct ttccaaacaa taaaattcct ttattaccgt attgctccca cgttaacctg
     7741 tttgctgcta aattacaatg tagagttgat aatgatttat agtgaactgt gctcttcttt
     7801 cagtaaaacc ccagggtccc ctgtgaggat gcaaatgttt ctttaaacaa agaaaaaaag
     7861 aaagaaagaa aaacactttt aacaatgaaa attagatgta catgtgtgat tcggtatgct
     7921 ttgccttctc cagcctaata ccagttacac tactgtttgt aaatgaaaag atatcttctt
     7981 cattaacggt ttggcccttt tcttagaaac tgcaggtgtt cctttatgaa cttgacccag
     8041 tgacatgaaa tgaactggaa aaggcaacct gtacctttaa tttaccagaa attgctttga
     8101 aattaatata ttgtcacatc tagagacctc ataaaagaga tagaataaac tattctttac
     8161 taggacaagt gcatattaaa taaaattatc ccaaccaaaa gaagtataag atgaaataga
     8221 gataacacag ggagtcttct ttgagcctca ctgccttagc ttgaaccaac aatccagaac
     8281 tagcagccat tcgggttaag gaagtgccat tgtcagggta gaatattaat atttggcttc
     8341 cactcttttt aagaacattg tgcctataca tatacacata cacagatata tacactatct
     8401 tccaggtatg aacagccaat catttattca ttcttgttta aaatgacttc agttgtatta
     8461 tataaatatt aaattaccat gataaaacat tcattcatgt gttcctacga attgctgctt
     8521 ttatcagagg tattgaaaag gactcatctg caaatatacc aatggctagt gtttgtggat
     8581 tgcttattgc acagcaagag ctatgctata agttttatag gtaaactctc catctatata
     8641 aatgaatgga attcctgtgt ctcctagttt aatcaggtat acaaggctga aaatttaaag
     8701 agcagtatct ggcagtttat accactgtat ttgctggtaa tatagacagc agttaagtca
     8761 caaattgact ctacttgcct aagccttgaa accttatttt cagggctatt gatcttgaat
     8821 atttagataa tcttactaca gattggactt tttgcttctg ttttaaagga aaaaaaagac
     8881 atcataatgg tttttacatg acaaattttt attcttcctg attataaagt cccaaaataa
     8941 agttctattt ctcttgcatt tgagcagagt taattttcca ggggtcacac ttgatttgtc
     9001 ttccagtcat gagcttttaa tggagaaaat tcccacagcc agataattat tagcaaagca
     9061 cataaattaa aggcaatttt tcatgttaaa aaaaactgct tctcacgtat aagtccttga
     9121 catagcactc ttttaaataa aaaaaaaaaa aattctagag tttttatgac agaatttgat
     9181 ttttaaaaat gacaatattt aaagtattac atgcaaaatg tccatttgaa acttgaaaga
     9241 tgaagttctt aagcgcccat ggctaattac ttttaatctc cacataagcg aaactgcatc
     9301 aggaaactgg acatgcaatc aattgaagag tgtgatcttc actacataag cacctcaatt
     9361 gttgagactc gctctgcccg caggttgaaa ctgtttctct gcagtaaccg agcaacccta
     9421 gctttggaat tacccagagc attttctttt atggatggca tgctttgtca gcaacatttc
     9481 cttcaggagg aatgattaaa agccttgaaa tcaattcagg acataaagat cttgaacttt
     9541 acggtgtcat ctgcctcctc tactgaccat tatttctaaa gtgatgtctg taaaagtata
     9601 taagctataa aaagtatatg aagctttatg taaggtatta cataaagcag agaaagctat
     9661 aaaaacatag aagctatttt ccttcttcat cttctttgtg tactctcaag tcatttaaca
     9721 gatgatttct gttttgtgtc ttctatcaat tacaagcact gatagcagcc atttcatgta
     9781 gtccagataa aggagaccat ttctcccatg ttatagctta tgcttcatgg atatggaact
     9841 aaagctagac ttggataact gtgctggcaa gttttatgtc aacttgacac aagctgaagc
     9901 aatttaaaca aataaagact ctcaatggag aaaatgccct cacaaaaaaa aaaaaaaa
//
