LOCUS       NM_178877               2028 bp    mRNA    linear   ROD 14-FEB-2025
DEFINITION  Mus musculus solute carrier family 9, subfamily B (NHA2, cation
            proton antiporter 2), member 2 (Slc9b2), transcript variant 1,
            mRNA.
ACCESSION   NM_178877
VERSION     NM_178877.7
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Anderegg,M.A., Albano,G., Hanke,D., Deisl,C., Uehlinger,D.E.,
            Brandt,S., Bhardwaj,R., Hediger,M.A. and Fuster,D.G.
  TITLE     The sodium/proton exchanger NHA2 regulates blood pressure through a
            WNK4-NCC dependent pathway in the kidney
  JOURNAL   Kidney Int 99 (2), 350-363 (2021)
   PUBMED   32956652
REFERENCE   3  (bases 1 to 2028)
  AUTHORS   Kondapalli,K.C., Todd Alexander,R., Pluznick,J.L. and Rao,R.
  TITLE     NHA2 is expressed in distal nephron and regulated by dietary sodium
  JOURNAL   J Physiol Biochem 73 (2), 199-205 (2017)
   PUBMED   27909897
  REMARK    GeneRIF: Data suggest that dietary salt elevates NHA2 expression in
            the nephron; NHA2 expression is localized apically to distal
            convoluted tubules and connecting tubules in the nephron; NHA2
            expression in the nephron partially overlaps in distribution with
            V-type ATPase.
REFERENCE   4  (bases 1 to 2028)
  AUTHORS   Deisl,C., Anderegg,M., Albano,G., Luscher,B.P., Cerny,D., Soria,R.,
            Bouillet,E., Rimoldi,S., Scherrer,U. and Fuster,D.G.
  TITLE     Loss of Sodium/Hydrogen Exchanger NHA2 Exacerbates Obesity- and
            Aging-Induced Glucose Intolerance in Mice
  JOURNAL   PLoS One 11 (9), e0163568 (2016)
   PUBMED   27685945
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2028)
  AUTHORS   Dickinson,M.E., Flenniken,A.M., Ji,X., Teboul,L., Wong,M.D.,
            White,J.K., Meehan,T.F., Weninger,W.J., Westerberg,H., Adissu,H.,
            Baker,C.N., Bower,L., Brown,J.M., Caddle,L.B., Chiani,F., Clary,D.,
            Cleak,J., Daly,M.J., Denegre,J.M., Doe,B., Dolan,M.E., Edie,S.M.,
            Fuchs,H., Gailus-Durner,V., Galli,A., Gambadoro,A., Gallegos,J.,
            Guo,S., Horner,N.R., Hsu,C.W., Johnson,S.J., Kalaga,S., Keith,L.C.,
            Lanoue,L., Lawson,T.N., Lek,M., Mark,M., Marschall,S., Mason,J.,
            McElwee,M.L., Newbigging,S., Nutter,L.M., Peterson,K.A.,
            Ramirez-Solis,R., Rowland,D.J., Ryder,E., Samocha,K.E.,
            Seavitt,J.R., Selloum,M., Szoke-Kovacs,Z., Tamura,M., Trainor,A.G.,
            Tudose,I., Wakana,S., Warren,J., Wendling,O., West,D.B., Wong,L.,
            Yoshiki,A., MacArthur,D.G., Tocchini-Valentini,G.P., Gao,X.,
            Flicek,P., Bradley,A., Skarnes,W.C., Justice,M.J., Parkinson,H.E.,
            Moore,M., Wells,S., Braun,R.E., Svenson,K.L., de Angelis,M.H.,
            Herault,Y., Mohun,T., Mallon,A.M., Henkelman,R.M., Brown,S.D.,
            Adams,D.J., Lloyd,K.C., McKerlie,C., Beaudet,A.L., Bucan,M. and
            Murray,S.A.
  CONSRTM   International Mouse Phenotyping Consortium; Jackson Laboratory;
            Infrastructure Nationale PHENOMIN, Institut Clinique de la Souris
            (ICS); Charles River Laboratories; MRC Harwell; Toronto Centre for
            Phenogenomics; Wellcome Trust Sanger Institute; RIKEN BioResource
            Center
  TITLE     High-throughput discovery of novel developmental phenotypes
  JOURNAL   Nature 537 (7621), 508-514 (2016)
   PUBMED   27626380
  REMARK    Erratum:[Nature. 2017 Nov 16;551(7680):398. doi:
            10.1038/nature24643.. PMID: 29144450]
REFERENCE   6  (bases 1 to 2028)
  AUTHORS   Hofstetter,W., Siegrist,M., Simonin,A., Bonny,O. and Fuster,D.G.
  TITLE     Sodium/hydrogen exchanger NHA2 in osteoclasts: subcellular
            localization and role in vitro and in vivo
  JOURNAL   Bone 47 (2), 331-340 (2010)
   PUBMED   20441802
  REMARK    GeneRIF: These data from NHA2-deficient mice suggest that NHA2 is
            dispensable for osteoclast differentiation and bone resorption both
            in vitro and in vivo.
REFERENCE   7  (bases 1 to 2028)
  AUTHORS   Lee,S.H., Kim,T., Park,E.S., Yang,S., Jeong,D., Choi,Y. and Rho,J.
  TITLE     NHE10, an osteoclast-specific member of the Na+/H+ exchanger
            family, regulates osteoclast differentiation and survival
            [corrected]
  JOURNAL   Biochem Biophys Res Commun 369 (2), 320-326 (2008)
   PUBMED   18269914
  REMARK    Erratum:[Biochem Biophys Res Commun. 2008 Aug 15;373(1):174]
REFERENCE   8  (bases 1 to 2028)
  AUTHORS   Battaglino,R.A., Pham,L., Morse,L.R., Vokes,M., Sharma,A.,
            Odgren,P.R., Yang,M., Sasaki,H. and Stashenko,P.
  TITLE     NHA-oc/NHA2: a mitochondrial cation-proton antiporter selectively
            expressed in osteoclasts
  JOURNAL   Bone 42 (1), 180-192 (2008)
   PUBMED   17988971
  REMARK    GeneRIF: NHA-oc/NHA2 displays the expected activities of a bona
            fide cation-proton antiporter and plays a key role(s) in normal
            osteoclast differentiation and function
REFERENCE   9  (bases 1 to 2028)
  AUTHORS   Pham,L., Purcell,P., Morse,L., Stashenko,P. and Battaglino,R.A.
  TITLE     Expression analysis of nha-oc/NHA2: a novel gene selectively
            expressed in osteoclasts
  JOURNAL   Gene Expr Patterns 7 (8), 846-851 (2007)
   PUBMED   17698421
  REMARK    GeneRIF: nha-oc is expressed by terminally differentiated
            osteoclasts.
REFERENCE   10 (bases 1 to 2028)
  AUTHORS   Lin,Q., Donahue,S.L., Moore-Jarrett,T., Cao,S., Osipovich,A.B. and
            Ruley,H.E.
  TITLE     Mutagenesis of diploid mammalian genes by gene entrapment
  JOURNAL   Nucleic Acids Res 34 (20), e139 (2006)
   PUBMED   17062627
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC104874.11.
            
            On Aug 24, 2022 this sequence version replaced NM_178877.6.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC090977.1, SRR13861890.14138.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849382
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-94                AC104874.11        141958-142051
            95-234              AC104874.11        151324-151463
            235-415             AC104874.11        152550-152730
            416-586             AC104874.11        156156-156326
            587-729             AC104874.11        157899-158041
            730-857             AC104874.11        158779-158906
            858-1033            AC104874.11        160364-160539
            1034-1140           AC104874.11        163262-163368
            1141-1290           AC104874.11        164038-164187
            1291-1399           AC104874.11        164853-164961
            1400-1536           AC104874.11        166686-166822
            1537-1757           AC104874.11        170624-170844
            1758-2028           AC104874.11        176540-176810
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 62.55 cM"
     gene            1..2028
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /note="solute carrier family 9, subfamily B (NHA2, cation
                     proton antiporter 2), member 2"
                     /db_xref="GeneID:97086"
                     /db_xref="MGI:MGI:2140077"
     exon            1..94
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            95..234
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     CDS             145..1788
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     mitochondrial sodium/hydrogen exchanger NHA2; NHE
                     domain-containing protein 2; sodium/hydrogen
                     exchanger-like domain-containing protein 2; mitochondrial
                     Na(+)/H(+) exchanger NHA2; Na(+)/H(+) exchanger-like
                     domain-containing protein 2; mitochondrial sodium/hydrogen
                     exchanger 9B2; Na+/H+ exchanger domain containing 2;
                     solute carrier family 9 subfamily B member 2;
                     soditlum/hydrogen exchanger-like domain-containing protein
                     2"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 9B2 isoform 1"
                     /protein_id="NP_849208.4"
                     /db_xref="CCDS:CCDS17854.1"
                     /db_xref="GeneID:97086"
                     /db_xref="MGI:MGI:2140077"
                     /translation="MEDEDKTAECQHSKPPTGITHEAPPHHELQEERVMSLRGTDRSE
                     PTEGSNLLTSGEKKPQDSPTEPNGLQSLRRFLACPPRGCLARVITNGTMVVLLWAMVW
                     SVTGPECLPGGNLFGIIILFYCSITGGKLFGLIKFPTLPPLPPLLGMLLAGFLLRNIP
                     VINDSVRIQHKWSSSLRSIALSVILVRAGLGLDSKALRKLKGVCVRLAMGPCIVEACA
                     SAILSHFLMGLPWQWGFILGFVVGAVSPAVVVPSMLLLQEGGYGVGKGIPTLLMAAGS
                     FDDILAITGFNTCLGVAFSTGSTVFNIFRGILEVVIGVAAGSFLGFFIQYFPSRDQDN
                     LVWKRAFLVLGFAVLAVFSSVYFSFPGSGGLCTLVMAFLAGMRWTDKKSEVEKVIAVT
                     WDVFQPLLFGLIGAEVSIVSLRAETVGLCVATLSIAVLIRILTTFLMVCFAGFNIKEK
                     IFISFAWLPKATVQAAIGSVALDTARSHGEKQLEDYGMDVLTVAFLAILITAPIGSLL
                     IGLLGPRVLQKSEHRTEEEVQGETSAHIQRKPEDSITEA"
     exon            235..415
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            416..586
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            587..729
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            730..857
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            858..1033
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1034..1140
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1290
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1291..1399
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1400..1536
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1537..1757
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     exon            1758..2028
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2002..2007
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /note="hexamer: AATAAA"
     polyA_site      2028
                     /gene="Slc9b2"
                     /gene_synonym="nha-oc; NHA2; nhaoc; NHE10; Nhedc2"
                     /note="major polyA site"
ORIGIN      
        1 acagcaccgc cctaacctag cacgccttgg taaacctagg ctcaactccc actcaacgga
       61 agccaggagg cctgcgcaga ctgccagcaa tgagagcagc tctggctcct catccctgag
      121 tcatcatctt cgaaattata agacatggag gatgaagata agacagctga atgtcagcat
      181 tcaaagccac ctacggggat cacacacgag gctcctccac atcacgaact acaggaagag
      241 agagtcatga gcctcagagg tacagacaga agtgaaccga cagaaggcag taacctgctg
      301 accagcggtg aaaaaaagcc acaggactca ccaacagaac ccaatggcct gcagagtctg
      361 aggcgattcc tggcctgccc tccacgaggc tgcctggcaa gagtgataac gaacggtacc
      421 atggttgttc ttctgtgggc catggtttgg tcagttaccg gccctgaatg tcttcctgga
      481 ggaaatctgt ttggaattat cattctgttc tattgttcca tcaccggagg taaacttttt
      541 ggactcatta agtttccaac attgcctcct ctgcctcctc ttcttggcat gctgctagct
      601 gggtttctct tgaggaatat cccagtcatc aatgatagcg tccggatcca acacaagtgg
      661 tcgtcatctt tgagaagcat agccctttct gtcattctgg ttcgtgctgg ccttggtcta
      721 gattcaaagg ccctgaggaa gctgaagggt gtgtgtgtgc gactggccat gggtccctgc
      781 atcgtggagg cgtgtgcttc tgcgattctc tcacacttcc tgatggggtt gccatggcaa
      841 tgggggttca tcctgggttt tgtcgtaggt gccgtgtccc cagctgtcgt ggtgccctcc
      901 atgctccttt tgcaggaagg aggctacggt gttggaaaag gtatcccaac cttactcatg
      961 gccgccggca gcttcgatga catcctggcc atcactggct tcaacacgtg cttaggcgtg
     1021 gccttttcca caggatctac agtttttaac atcttcagag gcatcttgga ggtggtaatt
     1081 ggtgtggcag ctggatcttt tcttgggttt tttatccagt acttcccgag cagggaccag
     1141 gacaacctcg tgtggaagcg agcctttctg gttctgggtt ttgctgtgct cgctgtgttc
     1201 agcagtgtgt attttagctt cccggggtct ggaggactct gcacgttggt catggctttc
     1261 ctagcaggca tgaggtggac tgacaagaag tcagaggtag aaaaggtcat tgcagttacc
     1321 tgggacgttt tccagcctct tctttttggc ctgattggag cagaggtttc cattgtgtct
     1381 ctcagagcag aaacggttgg cctttgtgtt gcaaccctca gcatcgcagt gcttatacga
     1441 attctgacta cattcctgat ggtgtgtttc gctggcttta acataaagga aaagatattt
     1501 atttcttttg cctggcttcc aaaggccacg gtccaggctg ccattggctc tgtggctctg
     1561 gacacggcaa gatcccacgg agagaagcag ctggaagact atgggatgga tgtgctgacg
     1621 gtggcatttt tggccatcct cattacagca ccaattggaa gcctactgat tggtttgctg
     1681 ggtcccaggg ttcttcagaa atctgaacat cgaaccgaag aggaggttca aggagagact
     1741 tctgcacaca ttcagaggaa gcctgaggat tccattacgg aagcctgatg gaccatgttt
     1801 accatcccaa cccaaaggtt ttggccctcc aacaaccggg acaactttac ttccctttga
     1861 ctcagaagaa aacttcccgt ggaatttcat aagcaaacaa attagaaagc tttacgctgc
     1921 taacagtacc tcaggtgttt acttcctcag aaagaccgga ggacaggtta cttcagaaag
     1981 tgagagaaag taatttggac aaataaaaca ttcacgattt tgttaaaa
//
