LOCUS       NM_028946               2253 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus solute carrier family 9, subfamily B (NHA1, cation
            proton antiporter 1), member 1 (Slc9b1), transcript variant 1,
            mRNA.
ACCESSION   NM_028946
VERSION     NM_028946.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2253)
  AUTHORS   Balbach,M., Hamzeh,H., Jikeli,J.F., Brenker,C., Schiffer,C.,
            Hansen,J.N., Neugebauer,P., Trotschel,C., Jovine,L., Han,L.,
            Florman,H.M., Kaupp,U.B., Strunker,T. and Wachten,D.
  TITLE     Molecular Mechanism Underlying the Action of Zona-pellucida
            Glycoproteins on Mouse Sperm
  JOURNAL   Front Cell Dev Biol 8, 572735 (2020)
   PUBMED   32984353
  REMARK    Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2253)
  AUTHORS   Holmes,R.S., Spradling-Reeves,K.D. and Cox,L.A.
  TITLE     Evolution of Vertebrate Solute Carrier Family 9B Genes and Proteins
            (SLC9B): Evidence for a Marsupial Origin for Testis Specific SLC9B1
            from an Ancestral Vertebrate SLC9B2 Gene
  JOURNAL   J Phylogenetics Evol Biol 4 (3) (2016)
   PUBMED   28868326
REFERENCE   4  (bases 1 to 2253)
  AUTHORS   Chen,S.R., Chen,M., Deng,S.L., Hao,X.X., Wang,X.X. and Liu,Y.X.
  TITLE     Sodium-hydrogen exchanger NHA1 and NHA2 control sperm motility and
            male fertility
  JOURNAL   Cell Death Dis 7 (3), e2152 (2016)
   PUBMED   27010853
  REMARK    GeneRIF: data revealed that NHA1 and NHA2 function as a key
            sodium-hydrogen exchanger responsible for sperm motility after
            leaving the cauda epididymidis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 2253)
  AUTHORS   Liu,T., Huang,J.C., Lu,C.L., Yang,J.L., Hu,Z.Y., Gao,F. and
            Liu,Y.X.
  TITLE     Immunization with a DNA vaccine of testis-specific sodium-hydrogen
            exchanger by oral feeding or nasal instillation reduces fertility
            in female mice
  JOURNAL   Fertil Steril 93 (5), 1556-1566 (2010)
   PUBMED   19409551
  REMARK    GeneRIF: Immunization with a DNA vaccine of testis-specific
            sodium-hydrogen exchanger (tsNHE) via oral feeding or nasal
            instillation reduced fertility in female mice.
REFERENCE   6  (bases 1 to 2253)
  AUTHORS   Liu,T., Huang,J.C., Zuo,W.L., Lu,C.L., Chen,M., Zhang,X.S.,
            Li,Y.C., Cai,H., Zhou,W.L., Hu,Z.Y., Gao,F. and Liu,Y.X.
  TITLE     A novel testis-specific Na+/H+ exchanger is involved in sperm
            motility and fertility
  JOURNAL   Front Biosci (Elite Ed) 2 (2), 566-581 (2010)
   PUBMED   20036903
  REMARK    GeneRIF: A novel testis-specific Na+/H+ exchanger, mtsNHE, is
            involved in sperm motility and fertility.
            Publication Status: Online-Only
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BX525256.1, AK029525.1, AC156618.5 and AV262918.1.
            
            On Nov 17, 2017 this sequence version replaced NM_028946.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK029525.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849384 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-8                 BX525256.1         1-8
            9-1033              AK029525.1         2-1026
            1034-1039           AC156618.5         63615-63620
            1040-2250           AK029525.1         1033-2243
            2251-2253           AV262918.1         259-261
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /db_xref="taxon:10090"
                     /chromosome="3"
                     /map="3 62.55 cM"
     gene            1..2253
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /note="solute carrier family 9, subfamily B (NHA1, cation
                     proton antiporter 1), member 1"
                     /db_xref="GeneID:74446"
                     /db_xref="MGI:MGI:1921696"
     exon            1..94
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            95..541
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     CDS             537..2234
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     sodium/hydrogen exchanger-like domain-containing protein
                     1; NHE domain-containing protein 1; Na(+)/H(+)
                     exchanger-like domain-containing protein 1; solute carrier
                     family 9 subfamily B member 1; sodium/hydrogen exchanger
                     9B1; Na+/H+ exchanger domain containing 1; testis specific
                     sodium-hydrogen exchanger"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 9B1 isoform 1"
                     /protein_id="NP_083222.2"
                     /db_xref="CCDS:CCDS17855.2"
                     /db_xref="GeneID:74446"
                     /db_xref="MGI:MGI:1921696"
                     /translation="MSEHDVESNKKDDGFQSSVTVEMSKDPDSFHEETVEPKPELKEP
                     EPKEPEPKEPERKEPERKEPERKEPERKEPERKVPGRRETQTKETQTTEIERKETKKK
                     RGTNSYCPPQGTINKTITDGAALIALWTLLWALIGQEVLPGGNLFGLVVIFYSAFLGG
                     KILEFIRIPVVPPLPPLIGMLLAGFTIRNVPIIYEFVHIPTTWSSALRNTALTIILVR
                     AGLGLDPQALKHLKGVCLRLSFGPCFLEACSAALFSHFIMNFPWQWGFLLGFVLGAVS
                     PAVVVPNMLMLQENGYGVEKGIPTLLVAASSMDDIVAITGFNTFLSIVFSSGSVISNI
                     LSSLRDVLIGVLVGIVMGVFVQYFPSGDQERLTQRRAFLVLSMCISAVLGCQHIGLHG
                     SGGLVTLVLSFMAAKRWAEEKVGIQKIVANTWNVFQPLLFGLVGTEVSVESLESKTIG
                     MCLATLGLALSVRILSTFVLMSFANFRFKEKVFIALSWIPKATVQAVLGPLALETARV
                     MAPHLEGYAKAVMTVAFLAILITAPNGALLIGILGPKILEQSEVTFPLKVELSNFHH"
     exon            542..602
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            603..900
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            901..1071
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1072..1214
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1215..1342
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1343..1518
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1625
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1626..1775
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1776..1884
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            1885..2021
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     exon            2022..2253
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2230..2235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /note="hexamer: ATTAAA"
     polyA_site      2251
                     /gene="Slc9b1"
                     /gene_synonym="1700094G20Rik; 4933424B12Rik;
                     4933425K02Rik; mtsNHE; Nhedc1"
                     /note="major polyA site"
ORIGIN      
        1 gtcctggagg ttgggtcctt ggctctcctg acctcctgca cgcgacgacg gttgctggtc
       61 cctgagcggg tgagagttgc taaaggaagt gtagcagttc ttgcaaataa gaaagaagag
      121 atctttccat cacgactgac agcaaaatta acccactggg cagatggtat ttgtacaaat
      181 cgtgaatccg gagaggtgaa ccttcacgtg cacctgtaat tgcagatgtg ttccaggatt
      241 ggtctctgat tttcggaagg ctctcattcc ttcccccaca cccctgacca ggattcctct
      301 tggctttatc gcagcggaca agcactcaac accaatgctt tttaaggatt aagggctgta
      361 attgcacaca cagtgtgtca gacactgcgc tctcctgagt cctgaccgtg ctctgtggtg
      421 gcgctcttct tggaattctt ccctattaag atgccataaa agcttgtcac ctttaggttt
      481 taaagttccc tgctgaaacg taagatatct taagactgca gacaaactcc tgaaagatga
      541 gtgagcacga cgtagaatca aacaagaagg atgatggttt ccaatcatct gtaactgttg
      601 agatgtccaa agatcctgat agttttcatg aagaaactgt ggaaccaaaa ccagaactga
      661 aggaaccgga accaaaggaa ccagaaccga aggaaccaga aaggaaggaa ccagaaagga
      721 aggaaccaga aaggaaggaa ccagaaagga aggaaccaga aaggaaggta ccaggaagaa
      781 gggaaacaca aacgaaggaa acacaaacaa ccgaaataga aaggaaggaa acaaaaaaga
      841 agagagggac aaatagttac tgtcctccac aaggaacaat aaataaaaca attacagatg
      901 gagctgcact cattgcacta tggactctac tctgggccct tataggtcaa gaagttctcc
      961 ctggtggaaa tttgtttgga cttgtagtta ttttttacag tgctttcctt gggggtaaaa
     1021 ttttagaatt cattagaata cctgtagtgc ctcccctccc acctctcatt gggatgttac
     1081 tggctggttt caccatcagg aatgttccga ttatttatga atttgtccat attcctacca
     1141 catggtcttc agctttaaga aacactgccc ttactattat cctagtacga gctgggcttg
     1201 gactggatcc acaggccttg aagcatctga agggagtctg tctgcgcttg tcatttggtc
     1261 catgcttcct agaagcctgc tcagccgctc tcttctccca cttcattatg aactttcctt
     1321 ggcaatgggg gttcctacta ggttttgtcc taggcgctgt ctctcctgct gttgtcgtcc
     1381 ccaacatgct aatgttgcag gagaatgggt atggtgttga gaaaggcatc ccgaccttgc
     1441 tggtggcagc tagcagcatg gacgacattg tggccatcac tggattcaac acattcttga
     1501 gcatagtctt ttcctcgggt agtgtgatta gcaacatcct atcgtccttg cgggacgttc
     1561 ttattggggt gctggtagga attgttatgg gagtttttgt tcagtatttt ccaagtggag
     1621 atcaggagag acttacgcag aggcgagcct tcctagttct gagtatgtgt atttctgctg
     1681 tcttaggctg ccagcacatt ggcttacatg gatctggagg actggtcaca ctagtattgt
     1741 cgttcatggc agcaaaaaga tgggctgaag agaaggttgg aatccaaaaa attgttgcaa
     1801 acacatggaa tgtttttcag ccgcttcttt ttggcttagt tggaacagaa gtatctgttg
     1861 aatctcttga atcaaaaacc attggcatgt gccttgctac cttgggtttg gcactatctg
     1921 ttcgaatttt atccactttt gtactgatgt cttttgctaa cttccgtttt aaggagaaag
     1981 tatttattgc tttatcatgg atccctaaag ctacagtcca ggctgtctta ggacctctgg
     2041 ctctagaaac agcaagggtc atggcacccc acttggaagg atacgcgaag gctgtgatga
     2101 cagtggcctt cctagccatc ctgattacag ctccaaatgg agccctcctc atcggcatac
     2161 tgggacccaa aatccttgaa cagagtgagg tgacattccc tttgaaagtt gagctctcga
     2221 acttccatca ttaaaaagct tgccatcatc acc
//
