LOCUS       NM_148929               4553 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus solute carrier family 9 (sodium/hydrogen exchanger),
            member 8 (Slc9a8), transcript variant 1, mRNA.
ACCESSION   NM_148929
VERSION     NM_148929.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4553)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 4553)
  AUTHORS   Bernardazzi,C., Xu,H., Tong,H., Laubitz,D., Figliuolo da Paz,V.,
            Curiel,L. and Ghishan,F.K.
  TITLE     An indisputable role of NHE8 in mucosal protection
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 319 (4), G421-G431 (2020)
   PUBMED   32755385
  REMARK    GeneRIF: An indisputable role of NHE8 in mucosal protection.
REFERENCE   3  (bases 1 to 4553)
  AUTHORS   Collin,G.B., Gogna,N., Chang,B., Damkham,N., Pinkney,J., Hyde,L.F.,
            Stone,L., Naggert,J.K., Nishina,P.M. and Krebs,M.P.
  TITLE     Mouse Models of Inherited Retinal Degeneration with Photoreceptor
            Cell Loss
  JOURNAL   Cells 9 (4), 931 (2020)
   PUBMED   32290105
  REMARK    Review article
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4553)
  AUTHORS   Wiebe,S.A., Plain,A., Pan,W., O'Neill,D., Braam,B. and
            Alexander,R.T.
  TITLE     NHE8 attenuates Ca2+ influx into NRK cells and the proximal tubule
            epithelium
  JOURNAL   Am J Physiol Renal Physiol 317 (2), F240-F253 (2019)
   PUBMED   31042050
  REMARK    GeneRIF: NHE8 regulates Ca(2+) uptake into the proximal tubule
            epithelium.
REFERENCE   5  (bases 1 to 4553)
  AUTHORS   Ingham,N.J., Pearson,S.A., Vancollie,V.E., Rook,V., Lewis,M.A.,
            Chen,J., Buniello,A., Martelletti,E., Preite,L., Lam,C.C.,
            Weiss,F.D., Powis,Z., Suwannarat,P., Lelliott,C.J., Dawson,S.J.,
            White,J.K. and Steel,K.P.
  TITLE     Mouse screen reveals multiple new genes underlying mouse and human
            hearing loss
  JOURNAL   PLoS Biol 17 (4), e3000194 (2019)
   PUBMED   30973865
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4553)
  AUTHORS   Xu,H., Zhang,B., Li,J., Wang,C., Chen,H. and Ghishan,F.K.
  TITLE     Impaired mucin synthesis and bicarbonate secretion in the colon of
            NHE8 knockout mice
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 303 (3), G335-G343 (2012)
   PUBMED   22575219
  REMARK    GeneRIF: the role of NHE8 in the intestine involves both sodium
            absorption and bicarbonate secretion
REFERENCE   7  (bases 1 to 4553)
  AUTHORS   Xu,H., Li,J., Chen,R., Zhang,B., Wang,C., King,N., Chen,H. and
            Ghishan,F.K.
  TITLE     NHE2X3 DKO mice exhibit gender-specific NHE8 compensation
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 300 (4), G647-G653 (2011)
   PUBMED   21252044
  REMARK    GeneRIF: intestinal NHE8 has a compensatory role in NHE2X3 DKO mice
            and this regulation is gender-dependent.
REFERENCE   8  (bases 1 to 4553)
  AUTHORS   Xu,H., Chen,H., Dong,J., Lynch,R. and Ghishan,F.K.
  TITLE     Gastrointestinal distribution and kinetic characterization of the
            sodium-hydrogen exchanger isoform 8 (NHE8)
  JOURNAL   Cell Physiol Biochem 21 (1-3), 109-116 (2008)
   PUBMED   18209477
  REMARK    GeneRIF: NHE8 is expressed along the gastrointestinal tract and
            NHE8 is a functional Na(+)/H(+) exchanger with kinetic
            characteristics that differ from other apically expressed NHE
            isoforms.
REFERENCE   9  (bases 1 to 4553)
  AUTHORS   Nakamura,N., Tanaka,S., Teko,Y., Mitsui,K. and Kanazawa,H.
  TITLE     Four Na+/H+ exchanger isoforms are distributed to Golgi and
            post-Golgi compartments and are involved in organelle pH regulation
  JOURNAL   J Biol Chem 280 (2), 1561-1572 (2005)
   PUBMED   15522866
REFERENCE   10 (bases 1 to 4553)
  AUTHORS   Goyal,S., Vanden Heuvel,G. and Aronson,P.S.
  TITLE     Renal expression of novel Na+/H+ exchanger isoform NHE8
  JOURNAL   Am J Physiol Renal Physiol 284 (3), F467-F473 (2003)
   PUBMED   12409279
  REMARK    GeneRIF: Has >50% identity to Drosophila melanogaster 'NHE1,'; NHE8
            is expressed in the proximal kidney tubule, where it may contribute
            to apical membrane ion transport
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL591762.13, AK049410.1 and
            BC058947.1.
            
            On Jan 31, 2015 this sequence version replaced NM_148929.2.
            
            Summary: This gene encodes a member of the Na+/H+ exchanger (NHE)
            family of integral membrane transporter proteins. The encoded
            protein is expressed on the apical membrane of the intestinal
            epithelial cells and plays an important role in mucosal protection.
            Loss of the encoded protein in mice results in a decrease in the
            number of goblet and mucin-positive cells, disorganization of the
            mucin layer, and a decrease in mucosal pH in the colon. Alternative
            splicing of this gene results in multiple transcript variants.
            [provided by RefSeq, Jan 2015].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC058947.1, AK049410.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-11                AL591762.13        113534-113544
            12-4521             AK049410.1         2-4511
            4522-4553           BC058947.1         4486-4517
FEATURES             Location/Qualifiers
     source          1..4553
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 87.22 cM"
     gene            1..4553
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /note="solute carrier family 9 (sodium/hydrogen
                     exchanger), member 8"
                     /db_xref="GeneID:77031"
                     /db_xref="MGI:MGI:1924281"
     exon            1..56
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     CDS             43..1773
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /note="isoform a is encoded by transcript variant 1;
                     sodium/hydrogen exchanger 8; sodium/proton exchanger NHE8;
                     Na(+)/H(+) exchanger 8"
                     /codon_start=1
                     /product="sodium/hydrogen exchanger 8 isoform a"
                     /protein_id="NP_683731.1"
                     /db_xref="CCDS:CCDS38339.1"
                     /db_xref="GeneID:77031"
                     /db_xref="MGI:MGI:1924281"
                     /translation="MAEEEFSNTTHETFNFTLHTTLGVTTKLVLPTPAKPILPVQTGE
                     QAQQEEQSSGMTIFFSLLVLAICIILVHLLIRYRLHFLPESVAVVSLGILMGAVIKVI
                     EFKKLANWKEEEMFRPNMFFLLLLPPIIFESGYSLHKGNFFQNIGSITLFAVFGTAIS
                     AFVVGGGIYFLGQADVISKLNMTDSFAFGSLISAVDPVATIAIFNALHVDPVLNMLVF
                     GESILNDAVSIVLTNTAEGLTRKHMSDVSGWQTFSQALGYFLKMFFGSAALGTLTGLI
                     SALVLKHIDLRKTPSLEFGMMIIFAYLPYGLAEGISLSGIMAILFSGIVMSHYTHHNL
                     SPVTQILMQQTLRTVAFLCETCVFAFLGLSIFSFPHKFEISFVIWCIVLVLFGRAVNI
                     FPLSYLLNFFRDHKITPKMMFIMWFSGLRGAIPYALSLHLGLEPMEKRQLIGTTTIVI
                     VLFTILLLGGSTMPLIRLVDIEDARARRRSKKDVNLSKTEKMGNAIESEHLSELTEEE
                     YEAHYIRQQDLKGFMWLDAKYLNPFFTRRLTQEDLHHGRIQMKSLTNKWYEEVRQGPS
                     GSEDDEQELF"
     exon            57..235
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            236..316
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            317..375
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            376..459
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            460..561
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            562..596
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            597..740
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            741..879
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            880..985
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            986..1102
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1103..1185
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1186..1297
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1298..1518
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1519..1665
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1666..1813
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     exon            1814..4523
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4499..4504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /note="hexamer: AATAAA"
     polyA_site      4523
                     /gene="Slc9a8"
                     /gene_synonym="1200006P13Rik; 6430709P13Rik; NHE-8; NHE8"
                     /note="major polyA site"
ORIGIN      
        1 ccgaactctg tagtctcgga agctcgcagg ataggggaga agatggcgga ggaggagttc
       61 tccaacacaa cccatgagac cttcaacttc acccttcaca ccaccttggg tgtcaccacg
      121 aagctggtgc tgccgacacc tgccaagcct atccttccgg tgcagacggg agagcaggcc
      181 cagcaggagg agcagtcgag cggcatgacc attttcttca gcctcctcgt cctagccatc
      241 tgcatcatcc tggtgcacct gctgattcgg taccgcctgc actttctgcc tgagagcgtg
      301 gctgtggttt ctctaggtat tctcatggga gcagtaataa aagttataga gtttaaaaag
      361 ctggcaaatt ggaaggagga agaaatgttt cgccccaaca tgtttttcct tctcttgctc
      421 ccacctatta tctttgagtc aggatactca ctgcacaagg ggaacttctt tcagaacatc
      481 ggttccatca ctctcttcgc tgtctttgga acggcaatct cggcttttgt agtaggtgga
      541 ggaatttact ttctgggtca ggctgatgta atctctaaac tcaacatgac agacagtttc
      601 gcatttggct ccctgatatc tgcagtcgac ccagtcgcca ctattgcgat tttcaacgca
      661 ctgcatgtgg accctgtgct caacatgctg gtgtttggag aaagcattct caacgatgca
      721 gtctccatcg tcctcaccaa cacggctgaa ggtctaacaa gaaaacatat gtcagatgtc
      781 agtgggtggc aaacgttctc acaggccctt ggctacttcc tcaaaatgtt ctttggctcc
      841 gcagcacttg gcacgctgac tggcttgatt tcagcattag tgttgaagca cattgacctg
      901 aggaaaacgc cttccctgga gtttggcatg atgatcattt ttgcttacct gccatatggg
      961 ctggcagaag gaatctcact ctcaggcatc atggcgatcc tgttttctgg catcgtgatg
     1021 tcacactata cacaccataa cctctcccca gtcacccaga tcctcatgca gcagactctc
     1081 cgaactgtgg ccttcctgtg tgaaacgtgt gtgtttgcat ttcttggcct ctccattttt
     1141 agtttccctc acaagtttga aatttccttt gtcatctggt gcatagtgct ggtcctgttt
     1201 ggcagagccg tcaacatctt ccctctgtcc tacctgctga acttcttccg agatcacaaa
     1261 atcaccccca agatgatgtt catcatgtgg ttcagtggtc ttcgaggggc catcccctat
     1321 gcactaagcc tgcacctggg cctggagccc atggagaagc gtcagctcat cggcaccacc
     1381 accatcgtca tcgtgctctt caccatcctg ctgctgggcg gcagcaccat gcccctcatc
     1441 cgcctcgtgg acatcgagga tgcccgggct cgccgaagga gcaagaaaga cgtcaacctc
     1501 agcaagactg agaagatggg taatgccatc gagtcagagc acctgtcaga gctcactgaa
     1561 gaagagtacg aagctcacta catcagacag caggacctca aaggcttcat gtggctggat
     1621 gccaagtacc tgaacccctt cttcacacgg cggctgacac aggaggacct ccaccatggc
     1681 cgcatccaga tgaagagcct caccaataag tggtatgagg aggtccgtca gggaccgtca
     1741 ggctctgagg atgatgagca ggagttgttc tgagcccaca gtgcctcact cagcctcctc
     1801 agaacacaag atggcccgca gaggacggca gttaccagcc tccttcctgc ttctctgcca
     1861 ggccacagag aaagccttgg aaggctgctt gctcatctca caccatgcct gggccatgcc
     1921 ttcctcacct ctggagcagc agctggcagg cacatctgcc atttgtcctg gaattgtcac
     1981 ggtgccttgg tgtcagcagc agtaacttgg tgccaaggcc aagagagaac agcccctggt
     2041 ctgcgtctgt gtccccaaga gagaggctga aggactttct cgacatggtc ctggcacttc
     2101 ccagcctgct tttgggaagg ctcctatggc ccctgtgggc acaatgaaaa tgtttacact
     2161 gatgcatggc agggttcagc ctgaatgctt ctagcaaagg tgttcaccca tcccctgggt
     2221 ttggagtgtt ctctgaagag acaaaatgga acccaagtgt gggagtcacc tccacgtccc
     2281 ctctcctatc tcccactgcc tcatctctca ttcttctgtc tccccagcgg cctctttcta
     2341 gaccctgtaa tgtgaagctc cttccaccca gctgctggtc agagcccagc tttgtctttt
     2401 accagtttct ggagcttggg tcctctgccc tccatgccac ggccccagct ctccagggca
     2461 ctggggccgt gctctccact tgggccactt ggattccggg gactttgggt cacagaaaca
     2521 cacacccatg catgtcgcag gataaggggc tggatcctac cacaagcaat ggttgttgcc
     2581 tgatgtcctc acctgccctt ttacgagtgg tgccctctgc ccatccgtgg cccttcagcg
     2641 actacccatg tgggccttca tccgccactg tactctgggt ggcttagttg tatatggccc
     2701 tgactcacaa tggggaccaa gtaggccttg atgcaggtgt ttctaacctc aggtactgtg
     2761 gttctgatgt tgggaaggac agccaggtct ctaatgccct gccacctcac tgtgctgcag
     2821 aagtcggtga agttggaaac atgcagtttg cttctgtaca gcagctcacg tgtccttggc
     2881 aaagccaagg tatggtcctc ttccttgtct ctcaccttag ccctggaact ggagccgctc
     2941 actttgtggc cttgtgtaat gatggggtta aggggctgtg ttcaggctgg gttgttctct
     3001 ttctgagaac tcaggttcca tctttgagat gaagaatcag ggtatcattc cgttggcccc
     3061 ttgtctccag gtctcctgtc cctggtccat cattcttggc ccctccttct agctgagacc
     3121 tgaagccagc ttcccagagc ttggtgagaa tgttagctct gcacaccagc ttcactgagc
     3181 accagctctg ctgagcacca ggaggtgcct ctgcctcttg ctggaggaca taagcttagc
     3241 accctgagag actgtccagc ccttcagagc cattggccag ggagcaaagt gctagatgcc
     3301 tttccttttt cagacgtttt acctgagaga caaactgctg ccagcacctc tcagacctgc
     3361 tcgttctgat gtgtgcgtgc ggtggcaaaa gcgcagatgc tacaagatgc tgccccagtt
     3421 gcatttcagg tctaggccaa ggggcaggcc agctgggcac acagaagcta atatacttaa
     3481 gagtagggag ggacatgggt gcccttgggt acctggcact ttagccagta agcatgccac
     3541 cctgtacttg ctggtggtcc atccctgcac tatttccaag ccaggtcttg ggatcagtga
     3601 cttgcttcct gcagtgctgt ggtcacaggc ccctccagtc agccatgtct agcagcttgg
     3661 caggctgagg ggctcggtgt gcagatttct ttttgtgcag cagaatgcca agagactgag
     3721 cagagagtct tcttaaaacc taccctaagg gctgccaggt ccatgtgccc gtggtaggtc
     3781 tggggccagg cagctgctgc tgtcctgatc ccccctccct tagctatgga tccttgtagt
     3841 agaggtcagc cgctcctggc gcaaacagga tccagtagct gtctgccacc cacgaggttt
     3901 ctaagatacc atgtggagac agggtcctcc ccatcctttt cagaaatagc agcttgtttt
     3961 ctctgctggc atgtgtccag ggcaccaggt cccagcaggg ggaggcagac tgttaactga
     4021 acgcgcatag gaacaggagg cagcgagagc ctgtgggagg aagggccttc tagtgaccct
     4081 gcttctccat cctgcctccc tcctgtccac ccagtttcag agttgggagg ggaggagcca
     4141 tggctccctc acccaggctt ggtggctgtg gccctgtttt agagccatgg cagcacaggc
     4201 tggtgagtgg tagagttgta ctgaaggctc tcttcacaag cctgggtttc tttcttctct
     4261 gacatggagg gttttggaag ccctagtttt ctgttgtgat ttaaggagac tggccgggag
     4321 ccattgttca aggtccctga ggcctttgct gctttgggaa gggcctgggg tctgagcttg
     4381 gggggggggg aagctgtcgc tccctcatct ggagcttccc tcttgcctgt ctactcccct
     4441 caccctcact gctgaattgg gggaagggtg ccccaggtca catgactgga tagttagaaa
     4501 taaagtgcta tgtagtcagc tgaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaa
//
