LOCUS       NM_133221               2893 bp    mRNA    linear   ROD 17-SEP-2024
DEFINITION  Mus musculus solute carrier family 8 (sodium/lithium/calcium
            exchanger), member B1 (Slc8b1), transcript variant 1, mRNA; nuclear
            gene for mitochondrial product.
ACCESSION   NM_133221
VERSION     NM_133221.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Taha,M., Assali,E.A., Ben-Kasus Nissim,T., Stutzmann,G.E.,
            Shirihai,O.S., Hershfinkel,M. and Sekler,I.
  TITLE     NCLX controls hepatic mitochondrial Ca2+ extrusion and couples
            hormone-mediated mitochondrial Ca2+ oscillations with
            gluconeogenesis
  JOURNAL   Mol Metab 87, 101982 (2024)
   PUBMED   38960129
  REMARK    GeneRIF: NCLX controls hepatic mitochondrial Ca[2+] extrusion and
            couples hormone-mediated mitochondrial Ca[2+] oscillations with
            gluconeogenesis.
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2893)
  AUTHORS   Meng,T.G., Guo,J.N., Zhu,L., Yin,Y., Wang,F., Han,Z.M., Lei,L.,
            Ma,X.S., Xue,Y., Yue,W., Nie,X.Q., Zhao,Z.H., Zhang,H.Y., Sun,S.M.,
            Ouyang,Y.C., Hou,Y., Schatten,H., Ju,Z., Ou,X.H., Wang,Z.B.,
            Wong,C.C.L., Li,Z. and Sun,Q.Y.
  TITLE     NLRP14 Safeguards Calcium Homeostasis via Regulating the K27
            Ubiquitination of Nclx in Oocyte-to-Embryo Transition
  JOURNAL   Adv Sci (Weinh) 10 (27), e2301940 (2023)
   PUBMED   37493331
REFERENCE   4  (bases 1 to 2893)
  AUTHORS   Cabral-Costa,J.V., Vicente-Gutierrez,C., Agulla,J., Lapresa,R.,
            Elrod,J.W., Almeida,A., Bolanos,J.P. and Kowaltowski,A.J.
  TITLE     Mitochondrial sodium/calcium exchanger NCLX regulates glycolysis in
            astrocytes, impacting on cognitive performance
  JOURNAL   J Neurochem 165 (4), 521-535 (2023)
   PUBMED   36563047
  REMARK    GeneRIF: Mitochondrial sodium/calcium exchanger NCLX regulates
            glycolysis in astrocytes, impacting on cognitive performance.
REFERENCE   5  (bases 1 to 2893)
  AUTHORS   Katoshevski,T., Bar,L., Tikochinsky,E., Harel,S., Ben-Kasus
            Nissim,T., Bogeski,I., Hershfinkel,M., Attali,B. and Sekler,I.
  TITLE     CKII Control of Axonal Plasticity Is Mediated by Mitochondrial Ca2+
            via Mitochondrial NCLX
  JOURNAL   Cells 11 (24), 3990 (2022)
   PUBMED   36552754
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2893)
  AUTHORS   Ritter,M., Sui,Z., Philipson,K.D., Li,F., Spitzer,K.W., Ishida,H.
            and Barry,W.H.
  TITLE     Ca2+ sparks induced by Na/Ca exchange
  JOURNAL   Cell Calcium 34 (1), 11-17 (2003)
   PUBMED   12767888
  REMARK    GeneRIF: Ca(2+) entering the myocyte via NCX can cause Ca(2+)
            sparks which are similar to those elicited by electrical
            stimulation. However, Ca(2+) influx on NCX is much less efficient
            in inducing Ca(2+) sparks than Ca(2+) influx via I(Ca,L).
REFERENCE   7  (bases 1 to 2893)
  AUTHORS   Burdakov,D., Liss,B. and Ashcroft,F.M.
  TITLE     Orexin excites GABAergic neurons of the arcuate nucleus by
            activating the sodium--calcium exchanger
  JOURNAL   J Neurosci 23 (12), 4951-4957 (2003)
   PUBMED   12832517
  REMARK    GeneRIF: An electrophysiologically distinct subset of arcuate
            nucleus neurons coexpress orexin receptors and glutamate
            decarboxylase-67 and are excited by orexin, activating a
            sodium-calcium exchange current, thereby depolarizing the cell and
            increasing its firing
REFERENCE   8  (bases 1 to 2893)
  AUTHORS   Fischer,K.G., Jonas,N., Poschenrieder,F., Cohen,C., Kretzler,M.,
            Greiber,S. and Pavenstadt,H.
  TITLE     Characterization of a Na(+)-Ca(2+) exchanger in podocytes
  JOURNAL   Nephrol Dial Transplant 17 (10), 1742-1750 (2002)
   PUBMED   12270979
  REMARK    GeneRIF: Presence of a Na(+)-Ca(2+) exchanger in podocytes and its
            regulation by PKC.
REFERENCE   9  (bases 1 to 2893)
  AUTHORS   Perelygin,A.A., Scherbik,S.V., Zhulin,I.B., Stockman,B.M., Li,Y.
            and Brinton,M.A.
  TITLE     Positional cloning of the murine flavivirus resistance gene
  JOURNAL   Proc Natl Acad Sci U S A 99 (14), 9322-9327 (2002)
   PUBMED   12080145
REFERENCE   10 (bases 1 to 2893)
  AUTHORS   Kopper,K.L. and Adorante,J.S.
  TITLE     Regulation of intracellular calcium in N1E-115 neuroblastoma cells:
            the role of Na(+)/Ca(2+) exchange
  JOURNAL   Am J Physiol Cell Physiol 282 (5), C1000-C1008 (2002)
   PUBMED   11940515
  REMARK    GeneRIF: data provide evidence for the presence of an electrogenic
            Na(+)/Ca(2+) exchanger that is capable of regulating [Ca(2+)](i)
            after release of Ca(2+) from cell stores
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AF261233.1, AK028537.1 and BP766040.1.
            
            On Mar 21, 2008 this sequence version replaced NM_133221.1.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF261233.1, AK028537.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-206               AF261233.1         1-206
            207-470             AK028537.1         152-415
            471-814             AF261233.1         471-814
            815-1031            AK028537.1         760-976
            1032-1041           AF261233.1         1032-1041
            1042-2872           AK028537.1         985-2815
            2873-2893           BP766040.1         1-21                c
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 60.63 cM"
     gene            1..2893
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /note="solute carrier family 8 (sodium/lithium/calcium
                     exchanger), member B1"
                     /db_xref="GeneID:170756"
                     /db_xref="MGI:MGI:2180781"
     exon            1..92
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            93..305
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     CDS             150..1907
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; solute carrier family 24 member 6;
                     Na(+)/K(+)/Ca(2+)-exchange protein 6; sodium/calcium
                     exchanger protein, mitochondrial; sodium/potassium/calcium
                     exchanger 6, mitochondrial; solute carrier family 24
                     (sodium/potassium/calcium exchanger), member 6; solute
                     carrier family 8 member B1; mitochondrial sodium/calcium
                     exchanger protein"
                     /codon_start=1
                     /product="mitochondrial sodium/calcium exchanger protein
                     isoform 1 precursor"
                     /protein_id="NP_573484.2"
                     /db_xref="CCDS:CCDS39238.1"
                     /db_xref="GeneID:170756"
                     /db_xref="MGI:MGI:2180781"
                     /translation="MASRWLALLWAPVFLCVALILETASGTGDPSTKAHGHIQFSAGS
                     VNQTAMADCRAVCGLNTSDRCDFVRRNPDCRSEAGYLDYLEGIFCYFPPNLLPLAITL
                     YVFWLLYLFLILGVTAAKFFCPNLSAISTNLKLSHNVAGVTFLAFGNGAPDIFSALVA
                     FSDPRTAGLAIGALFGAGVLVTTVVAGGITILHPFMAASRPFLRDIAFYMVAVFLTFT
                     ALYLGRITLTWALGYLGLYVFYVVTVIICTWVYQRQRSRSLVHSISETPELLSESEED
                     QMSSNTNSYDYGDEYRPLLLGRETTVQILIQALNPLDYRKWRTQSISWRVLKVVKLPV
                     EFLLLLTVPVVDPDKDDRNWKRPLNCLQLVISPLVLVLTLQSGVYGIYEIGGLLPVWA
                     VVVIVGTALASVTFFATSNREPPRLHWLFAFLGFLTSALWINAAATEVVNILRSLGVI
                     FRLSNTVLGLTLLAWGNSIGDAFSDFTLARQGYPRMAFSACFGGIIFNILVGVGLGCL
                     LQIIRNHVVEVKLEPDGLLVWVLASALGLSLIFSLVSVPLQCFQLSKAYGLCLLLFYI
                     CFLVVVLLTEFGVIHLKKA"
     exon            306..458
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            459..511
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            512..569
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            570..675
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            676..843
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            844..951
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            952..1011
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            1012..1142
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            1143..1284
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1406
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            1407..1560
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            1561..1641
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            1642..1709
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     exon            1710..2874
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2848..2853
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /note="hexamer: AATAAA"
     polyA_site      2874
                     /gene="Slc8b1"
                     /gene_synonym="NCKX6; NCLX; Slc24a6"
                     /note="major polyA site"
ORIGIN      
        1 ctccgggcca ggactctagg ggcggaaggt tgtggcgctg gccatccggc tagaggaaga
       61 ctccgaggtc gcggatccag gccccgcccg aggcactaga gcagccagcc cgtgagcaga
      121 gagggctctg gtcaggcctc aaggggccca tggcaagcag atggctggct ctgctctggg
      181 ctcctgtctt cctctgtgtg gctctgatac tggagacggc gtctgggacc ggagacccat
      241 ccacaaaagc ccatggacac atccagtttt cagctggaag tgtcaaccag actgccatgg
      301 cggattgccg agccgtgtgt ggcctgaaca catctgatcg ctgtgacttt gtcaggagga
      361 atccggactg ccgcagcgag gcgggctacc tggactacct tgagggcatc ttctgctact
      421 tcccccccaa cctcctccct ctggccatca ccctctacgt tttctggctg ctttacctct
      481 ttctgatcct gggagtcacc gcggccaagt tcttctgccc taacctgtca gccatctcca
      541 ctaacctcaa actctcccac aacgtggcag gtgtcacctt cctggccttt ggaaatggcg
      601 ctccagacat cttcagtgct ttagtggctt tctcagaccc acgtactgcc ggcctggcca
      661 tcggggctct gtttggtgca ggggtgctgg tcaccactgt ggtggctgga ggcatcacca
      721 tcctgcatcc cttcatggct gcctccaggc ccttcctcag ggacatcgct ttctacatgg
      781 tggctgtgtt cctaaccttc actgcactct atctcggcag gatcacgctg acgtgggcgc
      841 tgggttacct gggcctctac gtgttctacg tggtcacggt catcatctgc acttgggtct
      901 accaacggca gcgaagcagg tctctggtcc actccatatc ggagacacca gagttgctgt
      961 ctgagtcaga ggaggaccag atgtcttcca acaccaacag ctatgactat ggagatgaat
     1021 accggcctct gttgctgggt cgggagacca ctgtccagat cctgatccaa gccctgaatc
     1081 ccttggacta caggaagtgg agaactcagt cgatatcctg gagggtcctg aaggtagtca
     1141 agttgcctgt ggagttcttg ttgctgctca cagtaccggt tgtggacccc gacaaggacg
     1201 atcggaattg gaaacggcca ctcaactgtc tgcagctggt catcagcccc ctggtcctgg
     1261 tcctgaccct gcagtcgggg gtctatggca tctatgagat cggcggtctc cttcctgtct
     1321 gggctgtggt ggtgatcgtg ggcacagcgc tggcttcagt gaccttcttt gccacgtcta
     1381 acagagaacc ccctagactg cactggctct ttgctttcct gggtttcctg accagtgccc
     1441 tgtggatcaa tgcagccgcc acagaggtgg tgaacatatt acggtccctg ggtgtgatct
     1501 tccgcctgag caacaccgtc ctagggctga ccctcctggc ctggggaaac agcattggag
     1561 atgccttctc agatttcacg ctggcccgcc aaggataccc tcggatggcc ttctccgcct
     1621 gtttcggggg catcatcttc aacatcctgg ttggtgtggg gctgggctgc ttgctgcaga
     1681 tcatcaggaa ccacgttgtg gaggtgaagc tggagccaga cggattactg gtgtgggtgc
     1741 tggccagtgc cctgggcctc agcttgatct tctccctggt ctccgtgccg cttcagtgtt
     1801 tccagctcag caaggcttac ggcctctgcc tcctcctctt ctacatctgt ttccttgttg
     1861 tggtcctgct cacagagttt ggggtgattc acctgaagaa ggcgtgactg aagctgcttg
     1921 gcctagaggt gtgggggcga ttctgctagc ctcctgaggg ggaggtgtgg ggagggggac
     1981 cctctgtggt ccccgtggat ctcctgagaa gatagtcact ggcagagctc tgcatgggtg
     2041 agaaggtcct gactgccggc acctaacagc cttagtgtgg ggatctggag gctggctttg
     2101 ctggggacaa tcccgggtag gaatgatggg atctaaatga caccggaggc tctgggggga
     2161 gggcagcctt tcagtcagcc cccatgcctg ctgggctctg ggcagccctg cagttccctc
     2221 tctggctctt ccactctctg tggggtcctg cgtacctaca gggtggcctg aaaacagact
     2281 ccacacgtgg aaacaagact gggtttctca gcttccgtgt caagtcagcc agaggaaaga
     2341 ggtcgagttg acagcagcag gcactgccct tcctagttgg tggctgccat gttggactgt
     2401 ggatctaaac acttctagag ctttgtggtc caaactctgg ctctcccgtc tgcaaaacag
     2461 gagccagcct gggctctgtg cctacctcaa ggggagctgg gggttggggg gactgaccct
     2521 ccccagggag gtcttacaag tagtgtgacc agtcttttgt ttgtttgttt ggttggttgt
     2581 tttttggaga cagggtttct ctgtgtagcc ctggctgtcc tggaactcac tctgtagacc
     2641 aggctggcct tgaactcaga aatctgcctg cctttgcctc ccaagtgctg ggactaaagg
     2701 tgtgtgccac cactgtccag cttgtgacca gccttttaaa agtgccactc actccctgac
     2761 tgccttcagc tgtaattaag agacttgact gttggggttt tttttttgtt tgtttgtttt
     2821 ttgtttttgt tttttgttgt ttccaaaaat aaaagatgag ttatttcaca cgtaaaaaaa
     2881 aaaaaaaaaa aaa
//
