LOCUS       NM_178703               4144 bp    mRNA    linear   ROD 18-FEB-2025
DEFINITION  Mus musculus solute carrier family 6 (neurotransmitter transporter,
            GABA), member 1 (Slc6a1), mRNA.
ACCESSION   NM_178703 XM_975926 XM_975959 XM_975998 XM_976031 XM_976071
            XM_976103
VERSION     NM_178703.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Guo,W., Rioux,M., Shaffo,F., Hu,Y., Yu,Z., Xing,C. and Gray,S.J.
  TITLE     AAV9/SLC6A1 gene therapy rescues abnormal EEG patterns and
            cognitive behavioral deficiencies in Slc6a1-/- mice
  JOURNAL   J Clin Invest 135 (3), e182235 (2024)
   PUBMED   39589822
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Patel,J.C., Sherpa,A.D., Melani,R., Witkovsky,P., Wiseman,M.R.,
            O'Neill,B., Aoki,C., Tritsch,N.X. and Rice,M.E.
  TITLE     GABA co-released from striatal dopamine axons dampens phasic
            dopamine release through autoregulatory GABAA receptors
  JOURNAL   Cell Rep 43 (3), 113834 (2024)
   PUBMED   38431842
REFERENCE   3  (bases 1 to 4144)
  AUTHORS   Pulcrano,S., De Gregorio,R., De Sanctis,C., Volpicelli,F.,
            Piscitelli,R.M., Speranza,L., Perrone-Capano,C., di Porzio,U.,
            Caiazzo,M., Martini,A., Giacomet,C., Medina,D., Awatramani,R.,
            Viggiano,D., Federici,M., Mercuri,N.B., Guatteo,E. and
            Bellenchi,G.C.
  TITLE     miR-218 Promotes Dopaminergic Differentiation and Controls Neuron
            Excitability and Neurotransmitter Release through the Regulation of
            a Synaptic-Related Genes Network
  JOURNAL   J Neurosci 43 (48), 8104-8125 (2023)
   PUBMED   37816598
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4144)
  AUTHORS   Lindquist,B.E., Voskobiynyk,Y., Goodspeed,K. and Paz,J.T.
  TITLE     Patient-derived SLC6A1 variant S295L results in an epileptic
            phenotype similar to haploinsufficient mice
  JOURNAL   Epilepsia 64 (10), e214-e221 (2023)
   PUBMED   37501613
REFERENCE   5  (bases 1 to 4144)
  AUTHORS   Wu,Y.J., Yi,X., Gu,X., Wang,Q., Jiang,Q., Li,Y., Zhu,M.X., Ding,J.,
            Li,W.G. and Xu,T.L.
  TITLE     Synaptic scaling of corticostriatal circuits underlies
            hyperactivity in GABA Transporter-1 deficient mice
  JOURNAL   iScience 26 (4), 106322 (2023)
   PUBMED   36968092
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4144)
  AUTHORS   Evans,J.E., Frostholm,A. and Rotter,A.
  TITLE     Embryonic and postnatal expression of four gamma-aminobutyric acid
            transporter mRNAs in the mouse brain and leptomeninges
  JOURNAL   J Comp Neurol 376 (3), 431-446 (1996)
   PUBMED   8956109
REFERENCE   7  (bases 1 to 4144)
  AUTHORS   Ruiz,M., Egal,H., Sarthy,V., Qian,X. and Sarkar,H.K.
  TITLE     Cloning, expression, and localization of a mouse retinal
            gamma-aminobutyric acid transporter
  JOURNAL   Invest Ophthalmol Vis Sci 35 (12), 4039-4048 (1994)
   PUBMED   7960586
REFERENCE   8  (bases 1 to 4144)
  AUTHORS   el Mestikawy,S., Giros,B., Pohl,M., Hamon,M., Kingsmore,S.F.,
            Seldin,M.F. and Caron,M.G.
  TITLE     Characterization of an atypical member of the Na+/Cl(-)-dependent
            transporter family: chromosomal localization and distribution in
            GABAergic and glutamatergic neurons in the rat brain
  JOURNAL   J Neurochem 62 (2), 445-455 (1994)
   PUBMED   8294906
REFERENCE   9  (bases 1 to 4144)
  AUTHORS   Liu,Q.R., Lopez-Corcuera,B., Mandiyan,S., Nelson,H. and Nelson,N.
  TITLE     Molecular characterization of four pharmacologically distinct
            gamma-aminobutyric acid transporters in mouse brain [corrected]
  JOURNAL   J Biol Chem 268 (3), 2106-2112 (1993)
   PUBMED   8420981
  REMARK    Erratum:[J Biol Chem 1993 Apr 25;268(12):9156]
REFERENCE   10 (bases 1 to 4144)
  AUTHORS   Liu,Q.R., Mandiyan,S., Nelson,H. and Nelson,N.
  TITLE     A family of genes encoding neurotransmitter transporters
  JOURNAL   Proc Natl Acad Sci U S A 89 (14), 6639-6643 (1992)
   PUBMED   1631167
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC155719.11.
            
            On Aug 26, 2022 this sequence version replaced NM_178703.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK042956.1, BC059080.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849388, SAMN01164131
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-103               AC155719.11        88123-88225
            104-153             AC155719.11        106404-106453
            154-478             AC155719.11        107212-107536
            479-610             AC155719.11        107849-107980
            611-711             AC155719.11        108096-108196
            712-821             AC155719.11        109307-109416
            822-954             AC155719.11        110136-110268
            955-1089            AC155719.11        113046-113180
            1090-1193           AC155719.11        113374-113477
            1194-1318           AC155719.11        113907-114031
            1319-1431           AC155719.11        115487-115599
            1432-1563           AC155719.11        115812-115943
            1564-1666           AC155719.11        117154-117256
            1667-1767           AC155719.11        118788-118888
            1768-1935           AC155719.11        119644-119811
            1936-4144           AC155719.11        120654-122862
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="6"
                     /map="6 53.05 cM"
     gene            1..4144
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /note="solute carrier family 6 (neurotransmitter
                     transporter, GABA), member 1"
                     /db_xref="GeneID:232333"
                     /db_xref="MGI:MGI:95627"
     exon            1..103
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            104..153
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            154..478
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     CDS             241..2040
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /note="solute carrier family 6 member 1;
                     gamma-aminobutyric acid (GABA-A) transporter 1"
                     /codon_start=1
                     /product="sodium- and chloride-dependent GABA transporter
                     1"
                     /protein_id="NP_848818.1"
                     /db_xref="CCDS:CCDS20434.1"
                     /db_xref="GeneID:232333"
                     /db_xref="MGI:MGI:95627"
                     /translation="MATDNSKVADGQISTEVSEAPVASDKPKTLVVKVQKKAGDLPDR
                     DTWKGRFDFLMSCVGYAIGLGNVWRFPYLCGKNGGGAFLIPYFLTLIFAGVPLFLLEC
                     SLGQYTSIGGLGVWKLAPMFKGVGLAAAVLSFWLNIYYIVIISWAIYYLYNSFTTTLP
                     WKQCDNPWNTDRCFSNYSLVNTTNMTSAVVEFWERNMHQMTDGLDKPGQIRWPLAITL
                     AIAWVLVYFCIWKGVGWTGKVVYFSATYPYIMLIILFFRGVTLPGAKEGILFYITPNF
                     RKLSDSEVWLDAATQIFFSYGLGLGSLIALGSYNSFHNNVYRDSIIVCCINSCTSMFA
                     GFVIFSIVGFMAHVTKRSIADVAASGPGLAFLAYPEAVTQLPISPLWAILFFSMLLML
                     GIDSQFCTVEGFITALVDEYPRLLRNRRELFIAAVCIVSYLIGLSNITQGGIYVFKLF
                     DYYSASGMSLLFLVFFECVSISWFYGVNRFYDNIQEMVGSRPCIWWKLCWSFFTPIIV
                     AGVFLFSAVQMTPLTMGSYVFPKWGQGVGWLMALSSMVLIPGYMAYMFLTLKGSLKQR
                     LQVMIQPSEDIVRPENGPEQPQAGSSASKEAYI"
     exon            479..610
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            611..711
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            712..821
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            822..954
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            955..1089
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1090..1193
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1318
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1319..1431
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1432..1563
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1564..1666
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1767
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1768..1935
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     exon            1936..4144
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4139..4144
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /note="hexamer: AATAAA"
     polyA_site      4144
                     /gene="Slc6a1"
                     /gene_synonym="A730043E01; GABATHG; GABATR; Gabt; Gabt1;
                     GAT-1; Gat1; XT-1; Xtrp1"
                     /note="major polyA site"
ORIGIN      
        1 agagagctga gaggttgcag gcgagctgcg cgagcccagg agcccaggcg cccgcgtcca
       61 agcgcggccg agcccggagc ggcccgcgca gccccggccg caggctctgt ggagaaagcc
      121 tttaggagaa gactcctagc agagatcgat taggctgcaa agctgctgtc cacgtggact
      181 ggagctgaca tctcgcgccc acctgccagg atccccgctg ccaagtttgt gctccgagac
      241 atggcgactg acaacagcaa ggtggctgat gggcagatct ctactgaggt cagcgaggcc
      301 cctgtggcca gcgacaagcc caaaaccctg gtagtcaagg tgcagaagaa ggccggggac
      361 ctccctgacc gggacacatg gaagggacgc ttcgacttcc tcatgtcctg cgtgggctat
      421 gccatcggcc tgggcaatgt gtggaggttc ccttacctct gtgggaaaaa cggtggcggg
      481 gccttcctaa tcccatattt cctgacgctc atctttgcgg gtgttcctct cttccttttg
      541 gagtgctccc taggccagta cacctccatt gggggcctgg gcgtatggaa gctggcgccc
      601 atgttcaagg gtgtgggcct cgcggcagct gtgctgtcct tctggctgaa catctactac
      661 atcgtcatca tctcctgggc catctactac ctgtacaact ccttcaccac gaccctgcca
      721 tggaaacagt gtgacaaccc gtggaacact gaccgctgct tctccaacta cagcctggtc
      781 aataccacca acatgaccag cgccgtggtg gagttctggg agcgcaacat gcaccagatg
      841 acagatggac tggacaagcc aggacagatc cgctggcctc tggccatcac actggccatt
      901 gcctgggtgc tcgtgtattt ctgcatctgg aagggtgttg gttggactgg aaaggtggtc
      961 tacttctcag ccacgtaccc ctacatcatg cttatcatcc tgttcttccg tggagtgacg
     1021 cttcccgggg ccaaggaggg gatcctcttc tacatcacac ccaacttccg aaagctgtct
     1081 gattctgagg tgtggcttga cgccgccacc cagatcttct tctcctacgg gctgggcctg
     1141 gggtccctga ttgctctggg aagctacaac tctttccaca acaatgtgta cagggactcc
     1201 atcatcgttt gctgcatcaa ctcctgcacc agcatgtttg ccggattcgt catcttctcc
     1261 atcgtgggct tcatggctca tgtcaccaag aggtccatag ctgatgtggc agcctcaggc
     1321 ccggggctgg cattcttggc gtaccctgag gctgtgacac agctacccat ctctcccctc
     1381 tgggctatcc tcttcttctc catgctgctg atgctgggca ttgacagcca gttctgtacc
     1441 gtggagggct tcatcactgc cctggtggac gagtacccca gacttctccg caatcgccgt
     1501 gaactcttca ttgctgccgt gtgcatcgtg tcctacctga ttggcctgtc taacatcacc
     1561 cagggtggca tttatgtctt caaactgttt gattattact ctgccagcgg catgagcttg
     1621 ctgttcctgg ttttcttcga gtgtgtctcc atttcctggt tttatggtgt caaccggttc
     1681 tatgacaaca tccaggagat ggttggctcc aggccctgca tctggtggaa gctgtgctgg
     1741 tcctttttca cacccatcat tgtggcgggc gtgtttctct tcagtgctgt gcagatgaca
     1801 ccactcacca tgggaagcta tgttttcccc aagtggggcc agggcgtggg ctggctcatg
     1861 gctctgtcct ccatggtgct catccccggg tacatggctt acatgttcct caccctgaag
     1921 ggctccctga agcagcgtct ccaggtcatg attcagccca gtgaagatat tgtgcgccct
     1981 gagaatggcc ctgagcagcc gcaggctggc agctcagcca gcaaggaggc ctacatctag
     2041 gggtgcagcc ccccatcacc cctacactgg cactctggac tggctgtacc cacacccctt
     2101 gaagactgaa gatactctct gtctccacct acctcaaggg gcaggtccag acaccatgac
     2161 catgcagaga ggggcggtgg gggacagtct gaccctgggt gggccctgag tgggcagccg
     2221 cctctggagg cttccgtaga ggccccttag caggagcagg tggctagcct ttgtcactgc
     2281 cactgtagct cctttttatg ctgccagaga gggtgatagc tgcggccaca cgctcctggc
     2341 ttttagtctt tttttttttt tttcagactg ttgtcctgtg ccccaactat agactgttat
     2401 ccagactttt ctgcccgcct tggctctgaa ctggtcatgg accggggctt cggcattttg
     2461 ttcatggccg ctgggagcca gcaggctctg cctcttcctt ctcaatttct ccttcctgag
     2521 gcagcttcca ggacccaccc ccagactgtt cacagatcag tgcggagagg gtcctctgtc
     2581 cctgtgaccc tgtgacccgg gcagctcagt gtcccctgcc ctacctcccc aggtggcatt
     2641 aacaacaaca gcccatccag aagggtcctg tgtactggga aggaacaaaa gaaatcacaa
     2701 gcacaattgc cttttttggt caccatccca ggactttccc caagtgggag ctgtggtcct
     2761 tctgagctgc ccagtttggc acaaacaatg accaaggaaa tcaaggtgaa ggttctaagt
     2821 caggaccaat ccagctcact ctggtttccc tcttagactg tcactgccac tctgttctgg
     2881 tgtcccccct cacctttctg gaaaactttc agatgtacac gcccactcat ggagcagggt
     2941 tactccagag gctccgccca ccctaccctg gcattaagct tcccttgcta gcctgttcta
     3001 gtggggacat cgcatgtccc ttctggcctt gggttctgtg ttggtgaagc cagcggagac
     3061 agtttctgga aagttccaga agctctgtct ctctcctctg cggagagggt ggactcccat
     3121 gtagcaaagc gtatgtctcg tcccgtagct tcttagcttg atagctcaca aactgtgttt
     3181 atgactaatc cttaataact atggtgaata actgtgaccg tgggtttttt gaatctcttg
     3241 tcattctcat ccagaagtga ccagcacacc agttcttgca ataagctatc tccctccctc
     3301 agagcattaa gcacactgta gagaatgcag acacgtatgc acatagaaat gcacacacat
     3361 gcatacccat cctcacatgt ggcatttagc gtcccgtgtg atattgtgta ggcaatctac
     3421 cagctcttcc cgaggccact tgtaacaggg ttgtgtggct gaggcatctg cctgctcggt
     3481 ggagactctg cgactgacca gcgtgcacag atgcctgtcg acagagtccc aggtgtggaa
     3541 tgcaaggacc ctccactgtg tctcgtggcc tcaaccccac cccaccccca cctgtgtagg
     3601 aagcccttta ggatgagggc aggaggtctc cttcttgctg ctcggtgttc tttgatgtga
     3661 aactgagaac aagtcttttt gagataaatg cagtgtattt catgtttgta agcacctctg
     3721 agatgtttgg caagaaatcc cctgatttcc acccaaactt accttataga gcacaacgtt
     3781 aaaggtcgta caattactgt gagaactgtg aatatgtgta actttttttt tcagtttttg
     3841 cccagaggga agaagataat tgtattatca tatatgcttt cttttttttg caataaggat
     3901 ttattctcag aacaccaagt aaatttatct ctatataaaa gtatatgtaa tatatgccta
     3961 ttcagactat atacagagcc tgttttaaaa aaattacagt attatttagt aaaattatct
     4021 gttctatgga ccaaatgtaa aatatttata catgaagatg tgttttaaat gtctatcaaa
     4081 tggaatcaca gctagaacac gggcgtcatg tacgtttcta agaatttaga ggaaaaataa
     4141 taaa
//
