LOCUS       NM_133254               2270 bp    mRNA    linear   ROD 12-DEC-2024
DEFINITION  Mus musculus solute carrier family 5 (sodium/glucose
            cotransporter), member 2 (Slc5a2), mRNA.
ACCESSION   NM_133254
VERSION     NM_133254.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Wang,D., Zhang,Q., Dong,W., Ren,S., Wang,X., Su,C., Lin,X.,
            Zheng,Z. and Xue,Y.
  TITLE     SGLT2 knockdown restores the Th17/Treg balance and suppresses
            diabetic nephropathy in db/db mice by regulating SGK1 via Na
  JOURNAL   Mol Cell Endocrinol 584, 112156 (2024)
   PUBMED   38278341
  REMARK    GeneRIF: SGLT2 knockdown restores the Th17/Treg balance and
            suppresses diabetic nephropathy in db/db mice by regulating SGK1
            via Na.
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 2270)
  AUTHORS   Shim,B., Stokum,J.A., Moyer,M., Tsymbalyuk,N., Tsymbalyuk,O.,
            Keledjian,K., Ivanova,S., Tosun,C., Gerzanich,V. and Simard,J.M.
  TITLE     Canagliflozin, an Inhibitor of the Na+-Coupled D-Glucose
            Cotransporter, SGLT2, Inhibits Astrocyte Swelling and Brain
            Swelling in Cerebral Ischemia
  JOURNAL   Cells 12 (18), 2221 (2023)
   PUBMED   37759444
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2270)
  AUTHORS   Chun,H.J., Kim,E.R., Lee,M., Choi,D.H., Kim,S.H., Shin,E.,
            Kim,J.H., Cho,J.W., Han,D.H., Cha,B.S. and Lee,Y.H.
  TITLE     Increased expression of sodium-glucose cotransporter 2 and
            O-GlcNAcylation in hepatocytes drives non-alcoholic steatohepatitis
  JOURNAL   Metabolism 145, 155612 (2023)
   PUBMED   37277060
  REMARK    GeneRIF: Increased expression of sodium-glucose cotransporter 2 and
            O-GlcNAcylation in hepatocytes drives non-alcoholic
            steatohepatitis.
REFERENCE   5  (bases 1 to 2270)
  AUTHORS   Unno,K., Taguchi,K., Takagi,Y., Hase,T., Meguro,S. and Nakamura,Y.
  TITLE     Mouse Models with SGLT2 Mutations: Toward Understanding the Role of
            SGLT2 beyond Glucose Reabsorption
  JOURNAL   Int J Mol Sci 24 (7), 6278 (2023)
   PUBMED   37047250
  REMARK    GeneRIF: Mouse Models with SGLT2 Mutations: Toward Understanding
            the Role of SGLT2 beyond Glucose Reabsorption.
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2270)
  AUTHORS   Jurczak,M.J., Lee,H.Y., Birkenfeld,A.L., Jornayvaz,F.R.,
            Frederick,D.W., Pongratz,R.L., Zhao,X., Moeckel,G.W., Samuel,V.T.,
            Whaley,J.M., Shulman,G.I. and Kibbey,R.G.
  TITLE     SGLT2 deletion improves glucose homeostasis and preserves
            pancreatic beta-cell function
  JOURNAL   Diabetes 60 (3), 890-898 (2011)
   PUBMED   21357472
  REMARK    GeneRIF: Prevention of renal glucose reabsorption by SGLT2 deletion
            reduced hyperglycemia, improved glucose intolerance, and increased
            glucose-stimulated insulin secretion in vivo.
REFERENCE   7  (bases 1 to 2270)
  AUTHORS   Vallon,V., Platt,K.A., Cunard,R., Schroth,J., Whaley,J.,
            Thomson,S.C., Koepsell,H. and Rieg,T.
  TITLE     SGLT2 mediates glucose reabsorption in the early proximal tubule
  JOURNAL   J Am Soc Nephrol 22 (1), 104-112 (2011)
   PUBMED   20616166
  REMARK    GeneRIF: SGLT2 mediates glucose reabsorption in the early proximal
            tubule and most of the glucose reabsorption by the kidney, overall.
REFERENCE   8  (bases 1 to 2270)
  AUTHORS   Tabatabai,N.M., Blumenthal,S.S., Lewand,D.L. and Petering,D.H.
  TITLE     Differential regulation of mouse kidney sodium-dependent
            transporters mRNA by cadmium
  JOURNAL   Toxicol Appl Pharmacol 177 (3), 163-173 (2001)
   PUBMED   11749115
REFERENCE   9  (bases 1 to 2270)
  AUTHORS   Pontoglio,M., Prie,D., Cheret,C., Doyen,A., Leroy,C., Froguel,P.,
            Velho,G., Yaniv,M. and Friedlander,G.
  TITLE     HNF1alpha controls renal glucose reabsorption in mouse and man
  JOURNAL   EMBO Rep 1 (4), 359-365 (2000)
   PUBMED   11269503
REFERENCE   10 (bases 1 to 2270)
  AUTHORS   Wiley,L.M., Lever,J.E., Pape,C. and Kidder,G.M.
  TITLE     Antibodies to a renal Na+/glucose cotransport system localize to
            the apical plasma membrane domain of polar mouse embryo blastomeres
  JOURNAL   Dev Biol 143 (1), 149-161 (1991)
   PUBMED   1702069
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC124566.5.
            
            On Jun 17, 2019 this sequence version replaced NM_133254.4.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK128900.1, BC022226.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849385 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            RefSeq Select criteria           :: based on single protein-coding
                                                transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-134               AC124566.5         23289-23422         c
            135-206             AC124566.5         22689-22760         c
            207-311             AC124566.5         22508-22612         c
            312-476             AC124566.5         21604-21768         c
            477-582             AC124566.5         21087-21192         c
            583-663             AC124566.5         20293-20373         c
            664-893             AC124566.5         19923-20152         c
            894-1029            AC124566.5         19041-19176         c
            1030-1137           AC124566.5         18718-18825         c
            1138-1288           AC124566.5         18435-18585         c
            1289-1457           AC124566.5         18153-18321         c
            1458-1673           AC124566.5         17855-18070         c
            1674-1803           AC124566.5         17227-17356         c
            1804-2270           AC124566.5         16675-17141         c
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="7"
                     /map="7 70.08 cM"
     gene            1..2270
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /note="solute carrier family 5 (sodium/glucose
                     cotransporter), member 2"
                     /db_xref="GeneID:246787"
                     /db_xref="MGI:MGI:2181411"
     exon            1..134
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..2027
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /note="Na(+)/glucose cotransporter 2; low affinity
                     sodium-dependent glucose cotransporter"
                     /codon_start=1
                     /product="sodium/glucose cotransporter 2"
                     /protein_id="NP_573517.1"
                     /db_xref="CCDS:CCDS21894.1"
                     /db_xref="GeneID:246787"
                     /db_xref="MGI:MGI:2181411"
                     /translation="MEQHVEAGSELGEQKVLIDNPADILVIAAYFLLVIGVGLWSMFR
                     TNRGTVGGYFLAGRSMVWWPVGASLFASNIGSGHFVGLAGTGAASGLAVAGFEWNALF
                     VVLLLGWLFVPVYLTAGVITMPQYLRKRFGGHRIRLYLSVLSLFLYIFTKISVDMFSG
                     AVFIQQALGWNIYASVIALLGITMIYTVTGGLAALMYTDTVQTFVILAGAFILTGYAF
                     HEVGGYSGLFDKYLGAMTSLTVSKDPSVGNISSTCYQPRPDSYHLLRDPVTGDLPWPA
                     LLLGLTIVSGWYWCSDQVIVQRCLAGKNLTHIKAGCILCGYLKLMPMFLMVMPGMISR
                     ILYPDEVACVVPEVCKRVCGTEVGCSNIAYPRLVVKLMPNGLRGLMLAVMLAALMSSL
                     ASIFNSSSTLFTMDIYTRLRPRAGDKELLLVGRLWVVFIVAVSVAWLPVVQAAQGGQL
                     FDYIQSVSSYLAPPVSAVFVLALFVPRVNEKGAFWGLVGGLLMGLARLIPEFFFGSGS
                     CVRPSACPALFCRVHYLYFAIILFICSGILTLGISLCTAPIPQKHLHRLVFSLRHSKE
                     EREDLDADELEGPAPAPVQNGGQECAMEMEEVQSPAPGLLRRCLLWFCGMSKSGSGSP
                     PPTTEEVAATTRRLEDISEDPRWARVVNLNALLMMTVAVFLWGFYA"
     exon            135..206
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            207..311
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            312..476
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            477..582
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            583..663
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            664..893
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1029
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1030..1137
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1138..1288
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1457
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1458..1673
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1674..1803
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     exon            1804..2270
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2250..2255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /note="hexamer: AATAAA"
     polyA_site      2270
                     /gene="Slc5a2"
                     /gene_synonym="Sglt2"
                     /note="major polyA site"
ORIGIN      
        1 agatgctgga gagaatggag caacacgtag aggcaggctc tgaacttggg gagcagaagg
       61 tcctgattga taatcctgct gacattctgg ttatcgctgc ctatttcctg ctggtcattg
      121 gtgttggctt gtggtctatg ttcagaacca atagaggcac agttggtggc tacttcctgg
      181 caggacggag catggtgtgg tggccggttg gagcctctct gttcgccagc aacatcggca
      241 gcggtcattt tgtgggcctg gcagggactg gcgcagcaag tggcttggcg gtggctggat
      301 ttgagtggaa tgcgctcttc gtggtgctgc tcctcggatg gctttttgtg ccagtgtatc
      361 tgaccgctgg tgtgatcaca atgcctcagt acctccgcaa gcgctttggt gggcaccgta
      421 ttcgcctcta cctgtccgtg ctctcgcttt ttttgtacat tttcaccaag atctcggtgg
      481 atatgttctc tggggcagta ttcattcaac aggccctggg ctggaacatt tacgcttcgg
      541 tcatcgctct cttgggcatc accatgattt atactgtgac aggagggctg gcggcactga
      601 tgtacacaga cactgtgcag accttcgtca ttcttgccgg ggccttcatc ctcactggtt
      661 atgctttcca tgaagtgggc gggtactcgg gtctcttcga caaatacctg ggagcaatga
      721 cttcactgac ggtgtccaag gatccatctg ttggcaacat ctccagcacc tgctaccagc
      781 cgaggcctga ctcctatcac ctgctgcgtg accctgtgac aggagacctg ccatggcctg
      841 cgctgctcct ggggcttacc attgtctcgg gctggtattg gtgcagcgat caggtaatag
      901 tgcagcggtg cctggctgga aagaatctga ctcacatcaa agctgggtgc atcttgtgtg
      961 gctacctgaa gctgatgccc atgttcctca tggtcatgcc aggcatgatc agccgcattc
     1021 tctacccaga tgaggtggca tgtgtggtac ctgaggtgtg taagcgggtg tgtggcactg
     1081 aggtgggctg ctctaacatc gcctacccac ggctcgtggt gaagctcatg cccaatggtc
     1141 tgcgcggact catgctggca gtcatgctgg ctgccctcat gtcttctctg gcatccatct
     1201 ttaacagcag tagcacgctc ttcaccatgg atatctacac gcgcctgcgg ccccgtgcag
     1261 gtgataagga gctgctgcta gttggaaggc tctgggtggt attcatcgtg gcggtgtccg
     1321 tggcttggct gccagtggtg caggcagctc agggtgggca gctcttcgat tacattcagt
     1381 ctgtctccag ctatctggca cctccagtgt ctgcggtctt tgtgcttgca ctctttgtgc
     1441 cccgtgttaa tgagaaggga gccttctggg gactagttgg gggcctgctg atgggcctag
     1501 ctcgtctcat acccgagttc ttctttggct cgggcagctg tgtgcgaccc tcagcgtgcc
     1561 cggcactctt ctgtcgggta cactacctct atttcgccat cattctcttc atctgctctg
     1621 gcatcctcac acttggaatc tccctgtgca ctgcgcccat ccctcagaag catctccatc
     1681 gcctggtttt cagtctccgg cacagcaagg aggagcggga ggacctggat gctgatgagt
     1741 tagaaggtcc agcccctgct cctgtgcaga acgggggcca ggaatgtgca atggagatgg
     1801 aagaggtcca gtccccggct ccaggcctgc tccgccggtg cctgctttgg ttctgtggga
     1861 tgagcaagag tgggtcaggg agtcctccgc ccactaccga ggaggtggcg gcaaccacca
     1921 ggcggctgga ggacatcagt gaggatcccc gctgggcacg agtagtcaac ctcaatgccc
     1981 tactcatgat gaccgtggct gtgttcctct ggggcttcta tgcataaagt cgagggtgtt
     2041 ggatgccatg agctacaacc aggccatgtt ggaccctcac aaagagtaag ggtgagcagc
     2101 ttggagtgga tcccagaaaa ggaacagggc aagaatacag caggaaggaa ccggttccct
     2161 tcctctttac ccggggtcca gtccatttga ttggttgtca cttcccacaa gatgatggcc
     2221 aattggtcat agaggtttgc ctatacaaaa ataaaactgc cctcctaaca
//
