LOCUS       NM_021530              11811 bp    mRNA    linear   ROD 20-MAY-2024
DEFINITION  Mus musculus solute carrier family 4 (anion exchanger), member 8
            (Slc4a8), transcript variant 1, mRNA.
ACCESSION   NM_021530
VERSION     NM_021530.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 11811)
  AUTHORS   Pena-Munzenmayer,G., George,A.T., Llontop,N., Mazola,Y.,
            Apablaza,N., Spichiger,C., Brauchi,S., Sarmiento,J., Zuniga,L.,
            Gonzalez,W. and Catalan,M.A.
  TITLE     K+-Driven Cl-/HCO3- Exchange Mediated by Slc4a8 and Slc4a10
  JOURNAL   Int J Mol Sci 25 (8), 4575 (2024)
   PUBMED   38674160
  REMARK    GeneRIF: K[+]-Driven Cl[-]/HCO3[-] Exchange Mediated by Slc4a8 and
            Slc4a10.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11811)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   3  (bases 1 to 11811)
  AUTHORS   Guo,P., Lim,R.C., Rajawasam,K., Trinh,T., Sun,H. and Zhang,H.
  TITLE     A methylation-phosphorylation switch controls EZH2 stability and
            hematopoiesis
  JOURNAL   Elife 13, e86168 (2024)
   PUBMED   38346162
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 11811)
  AUTHORS   Xu,J., Barone,S., Zahedi,K., Brooks,M. and Soleimani,M.
  TITLE     Slc4a8 in the Kidney: Expression, Subcellular Localization and Role
            in Salt Reabsorption
  JOURNAL   Cell Physiol Biochem 50 (4), 1361-1375 (2018)
   PUBMED   30355950
REFERENCE   5  (bases 1 to 11811)
  AUTHORS   Sinning,A., Radionov,N., Trepiccione,F., Lopez-Cayuqueo,K.I.,
            Jayat,M., Baron,S., Corniere,N., Alexander,R.T., Hadchouel,J.,
            Eladari,D., Hubner,C.A. and Chambrey,R.
  TITLE     Double Knockout of the Na+-Driven Cl-/HCO3- Exchanger and Na+/Cl-
            Cotransporter Induces Hypokalemia and Volume Depletion
  JOURNAL   J Am Soc Nephrol 28 (1), 130-139 (2017)
   PUBMED   27151921
REFERENCE   6  (bases 1 to 11811)
  AUTHORS   Chen,L.M., Haddad,G.G. and Boron,W.F.
  TITLE     Effects of chronic continuous hypoxia on the expression of SLC4A8
            (NDCBE) in neonatal versus adult mouse brain
  JOURNAL   Brain Res 1238, 85-92 (2008)
   PUBMED   18775686
  REMARK    GeneRIF: mouse brain exhibits marked developmental differences in
            the response of NDCBE protein expression to chronic continuous
            hypoxia
REFERENCE   7  (bases 1 to 11811)
  AUTHORS   Chen,L.M., Kelly,M.L., Parker,M.D., Bouyer,P., Gill,H.S.,
            Felie,J.M., Davis,B.A. and Boron,W.F.
  TITLE     Expression and localization of Na-driven Cl-HCO(3)(-) exchanger
            (SLC4A8) in rodent CNS
  JOURNAL   Neuroscience 153 (1), 162-174 (2008)
   PUBMED   18359573
  REMARK    GeneRIF: Thus, SLC4A8 is in a position to contribute substantially
            to pH(i) regulation in multiple CNS neurons.
REFERENCE   8  (bases 1 to 11811)
  AUTHORS   O'Shaughnessy,P.J., Abel,M., Charlton,H.M., Hu,B., Johnston,H. and
            Baker,P.J.
  TITLE     Altered expression of genes involved in regulation of vitamin A
            metabolism, solute transportation, and cytoskeletal function in the
            androgen-insensitive tfm mouse testis
  JOURNAL   Endocrinology 148 (6), 2914-2924 (2007)
   PUBMED   17332066
REFERENCE   9  (bases 1 to 11811)
  AUTHORS   Willnow,T., Antignac,C., Brandli,A., Christensen,E., Cox,R.,
            Davidson,D., Davies,J., Devuyst,O., Eichele,G., Hastie,N.,
            Verroust,P., Schedl,A. and Meij,I.
  TITLE     The European renal genome project: an integrated approach towards
            understanding the genetics of kidney development and disease
  JOURNAL   Organogenesis 2 (2), 42-47 (2005)
   PUBMED   19521566
REFERENCE   10 (bases 1 to 11811)
  AUTHORS   Wang,Z., Conforti,L., Petrovic,S., Amlal,H., Burnham,C.E. and
            Soleimani,M.
  TITLE     Mouse Na+: HCO3- cotransporter isoform NBC-3 (kNBC-3): cloning,
            expression, and renal distribution
  JOURNAL   Kidney Int 59 (4), 1405-1414 (2001)
   PUBMED   11260402
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BB626223.1, BB851903.1, AK173014.1, AC113587.16 and BP759706.1.
            
            On Aug 8, 2006 this sequence version replaced NM_021530.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK034237.1, AK173014.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-68                BB626223.1         2-69
            69-166              BB851903.1         66-163
            167-4601            AK173014.1         1-4435
            4602-11295          AC113587.16        151315-158008
            11296-11811         BP759706.1         27-542              c
FEATURES             Location/Qualifiers
     source          1..11811
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="15"
                     /map="15 56.39 cM"
     gene            1..11811
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /note="solute carrier family 4 (anion exchanger), member
                     8"
                     /db_xref="GeneID:59033"
                     /db_xref="MGI:MGI:1928745"
     exon            1..228
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     CDS             184..3453
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /note="isoform 1 is encoded by transcript variant 1;
                     electroneutral sodium bicarbonate exchanger 1; k-NBC3;
                     solute carrier family 4 member 8; electroneutral
                     Na+-driven Cl-HCO3 exchanger"
                     /codon_start=1
                     /product="electroneutral sodium bicarbonate exchanger 1
                     isoform 1"
                     /protein_id="NP_067505.2"
                     /db_xref="CCDS:CCDS37214.1"
                     /db_xref="GeneID:59033"
                     /db_xref="MGI:MGI:1928745"
                     /translation="MPAGSNEPDGVLSYQRPDEEAVVDQGGTSTILNIHYEKEELEGH
                     RTLYVGVRMPLGRQSHRHHRTHGQKHRRRGGRGKGASQGEEGLEALAHDTPSQRVQFI
                     LGTEEDEEHVPHELFTELDEICMKEGEDAEWKETARWLKFEEDVEDGGERWSKPYVAT
                     LSLHSLFELRSCLINGSVLLDMRASSIEEISDLILDQQELLRDLSDSVRVKVREALLK
                     KHHHQNERRRNNLIPIVRSFAEVGKKQSDPHSMDRDGQTVSPQSATNLEVKNGVNCEH
                     SPVDLSKVDLHFMKKIPTGAEASNVLVGEVDTLDRPIVAFVRLSPAVLLSGLTEVPIP
                     TRFLFILLGPVGKGQQYHEIGRSMATIMTDEIFHDVAYKAKERDDLLAGIDEFLDQVT
                     VLPPGEWDPSIRIEPPKNVPSQEKRKMPGVPNGNVCHIEPEPHGGHSGPELERTGRLF
                     GGLVLDVKRKAPWYWSDYRDALSLQCLASFLFLYCACMSPVITFGGLLGEATEGRISA
                     IESLFGASMTGIAYSLFAGQPLTILGSTGPVLVFEKILFKFCKDYALSYLSLRALIGL
                     WTAFLCIVLVATDASSLVCYITRFTEEAFASLICIIFIYEAIEKLIHLAETYPIHMHS
                     QLDHLSLYYCRCVLPENPNNHTLQYWKDHNILAAEVNWANLTVSECQEMHGEFMGSAC
                     GHHGPYTPDVLFWSCILFFATFIVSSTLKTFKTSRYFPTRVRSMVSDFAVFLTIFTMV
                     VLDFLIGVPSPKLQVPNVFKPTRDDRGWFINPIGPNPWWTVIAAIIPALLCTILIFMD
                     QQITAVIINRKEHKLKKGCGYHLDLLMVAVMLGVCSIMGLPWFVAATVLSITHVNSLK
                     LESECSAPGEQPKFLGIREQRVTGLMIFVLMGCSVFMTAVLKFIPMPVLYGVFLYMGV
                     SSLQGIQFFDRLKLFGMPAKHQPDFIYLRHVPLRKVHLFTLVQLTCLVLLWVIKASPA
                     AIVFPMMVLALVFVRKVMDLCFSKRELSWLDDLMPESKKKKLDDAKKKEEEEAEKMLD
                     IGGDKFPLESRKLLSSPGKSSSFRCDPSEINISDEMPKTTVWKALSINSGNTKEKSPF
                     N"
     exon            229..310
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            311..460
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            461..596
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            597..757
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            758..946
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1032
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1033..1190
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1191..1278
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1425
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1426..1526
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1701
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1702..1835
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            1836..2081
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2082..2187
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2188..2349
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2350..2463
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2464..2625
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2626..2877
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2878..2946
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            2947..3120
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3121..3252
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3253..3344
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3345..3440
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     exon            3441..11793
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3734..3739
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /note="hexamer: ATTAAA"
     polyA_site      3758
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /note="major polyA site"
     polyA_site      4607
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /note="major polyA site"
     polyA_site      4630
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /note="major polyA site"
     regulatory      7047..7052
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /note="hexamer: TATAAA"
     polyA_site      7069
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
     regulatory      11768..11773
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /note="hexamer: AATAAA"
     polyA_site      11791
                     /gene="Slc4a8"
                     /gene_synonym="C230026C11; kNBC-3; NDCBE"
                     /note="major polyA site"
ORIGIN      
        1 gattggctgc tgcgggcgcc tcgcgggctg atggctatga aggtggcgcc ggttgatggt
       61 tgaccgatcc ttggatccag ggtaggggtc gctgcgcgtg cgatcgtctc acactggact
      121 acagggcgcg gactgctgca gctcaacctg gaacctaccg gcgagaccag gctccgcccg
      181 gccatgcccg ccgggagcaa cgagccggac ggcgtcctca gctatcagag gccagatgag
      241 gaagctgtgg tggatcaggg tgggaccagc acaattctca acattcacta tgagaaagaa
      301 gagttggaag gtcatagaac tctgtatgtg ggggttcgga tgccgctggg gaggcagagc
      361 catcggcacc accgcactca tggccagaag caccggagac gaggagggcg gggcaaagga
      421 gccagccagg gcgaggaggg cttggaagcc ttggcccacg acaccccgtc tcagcgtgtt
      481 cagttcattc ttggcactga ggaagatgaa gaacatgtgc ctcatgagct cttcacagag
      541 ctggacgaga tctgtatgaa agagggggaa gatgctgagt ggaaggagac ggccaggtgg
      601 ctgaagtttg aagaggatgt ggaggacggg ggagagcgct ggagcaagcc ttacgtggcc
      661 acgctctctc tgcacagcct gtttgagctg aggagctgcc tcatcaacgg ctctgtgctt
      721 ctggacatgc gtgcaagtag catagaggag atctcagacc tgatcctgga ccagcaggag
      781 ctgctccgag acctgagcga cagcgtgagg gttaaagtgc gggaagccct gctgaagaag
      841 caccaccacc agaacgagag gaggaggaac aacctcatcc ccatcgtccg ctccttcgct
      901 gaggttggca agaagcagtc tgacccgcac tccatggaca gagatgggca aacggtgtct
      961 cctcagtcag ctacaaacct tgaagtgaag aatggtgtga actgtgagca cagccctgtg
     1021 gatctaagca aggtggatct tcacttcatg aaaaaaattc ccacgggggc agaggcctcc
     1081 aatgtcctgg tgggagaggt ggatacgctg gaccggccca tcgtggcctt cgtgaggctc
     1141 tccccggctg tgctgctctc tggcctgacg gaagtgccca tcccaacgag gtttttgttt
     1201 atcttactgg gcccagtggg aaaagggcag cagtaccatg agattggcag gtccatggcc
     1261 accatcatga cggacgagat ttttcatgat gtggcatata aagcaaagga acgcgatgac
     1321 ctcctggcag ggattgacga gttcctggac caggtgacgg tgcttccgcc aggggagtgg
     1381 gacccatcta ttagaattga gccacccaag aatgtccctt cccaggaaaa aaggaaaatg
     1441 cccggtgttc caaacggaaa cgtttgccac atagaaccag agccacatgg ggggcacagt
     1501 gggccagaac tggagcggac tgggcggctg tttgggggct tggtgctgga tgtcaagcgg
     1561 aaggctccct ggtactggag tgactaccgg gatgctctca gcttacagtg tctggcctcc
     1621 ttcctgttcc tgtactgtgc ctgcatgtcg cccgttatca ccttcggggg actgctggga
     1681 gaagccactg aaggacgcat aagtgcgatt gagtcgttgt ttggagcgtc catgacgggg
     1741 attgcctact ctctgtttgc tgggcagcct ctcaccatcc tgggaagcac ggggcccgtg
     1801 ctggtgtttg aaaagatttt gttcaaattc tgcaaggact atgccctttc gtacctgtcc
     1861 ctgcgggctc tcatcgggct gtggactgcc ttcctgtgca tcgtcctggt ggccacggat
     1921 gccagctccc tcgtctgcta cattacccgc ttcaccgagg aagcgtttgc ctccctgatt
     1981 tgtatcatct tcatctatga agccatagaa aagctgatcc acctggcaga gacctacccc
     2041 atccacatgc acagccagct ggaccacctg agcctctatt actgcaggtg tgtgctcccg
     2101 gagaatccaa acaaccacac cttacagtat tggaaggacc acaacatcct ggctgccgaa
     2161 gtgaactggg caaacctgac agtcagcgaa tgccaggaga tgcacgggga gttcatggga
     2221 tctgcctgcg gtcaccacgg accctacacg cccgacgtgc tcttttggtc ctgcattctg
     2281 ttcttcgcca ccttcatcgt ctccagcacc ttaaagacat ttaagacaag ccgctacttc
     2341 ccgaccaggg tccgctccat ggtgagtgac ttcgccgtgt tcctcaccat cttcaccatg
     2401 gtggtccttg actttctgat tggcgtcccg tcgccaaagc ttcaggttcc caatgtgttc
     2461 aagccaacaa gggacgatcg aggatggttt attaatccca ttggccccaa tccctggtgg
     2521 actgtgatag ccgcaattat cccagctctc ctgtgcacta tcctgatatt catggaccag
     2581 cagatcaccg ctgtcatcat taacaggaag gaacacaagc tcaagaaagg ctgtggctac
     2641 cacctggacc tgctcatggt ggccgtcatg ctgggagttt gctccatcat gggcctgcct
     2701 tggtttgtgg ccgcgaccgt cctgtccatt acccatgtga acagcctcaa gctggaatcc
     2761 gaatgctctg cccccgggga gcagcccaag ttcttgggca tccgggaaca gagagtcaca
     2821 gggcttatga tcttcgtgct gatgggctgc tcagtcttca tgacagctgt cttaaagttt
     2881 atcccgatgc cagtgctcta cggagttttc ctctacatgg gagtctcttc gctacaagga
     2941 atccagttct tcgaccgtct gaagcttttc gggatgcctg caaaacacca gccagacttc
     3001 atctacctgc ggcacgtgcc gctgcgcaag gtgcacctgt tcacgctagt gcagctcacc
     3061 tgcctcgtcc tgctctgggt catcaaggca tcgccggccg ccattgtttt cccaatgatg
     3121 gtgctggcct tggtcttcgt caggaaagtc atggatctct gcttctccaa gcgtgagcta
     3181 agctggctgg acgacctcat gcctgagagc aaaaagaaga agctggacga tgcgaagaag
     3241 aaggaggagg aggaggccga gaagatgttg gacattgggg gtgacaagtt tcccctggaa
     3301 agcaggaagc tgctaagtag tcctggcaag agcagcagct tcagatgtga cccttctgag
     3361 attaatatat cagatgaaat gcctaaaacc acggtctgga aggctctcag cataaattcc
     3421 ggaaatacaa aagaaaaaag ccccttcaac tgagagtctc tgctgagtgg aagatttgga
     3481 ccatgaggtg cctcggagaa gttctgggtt ttaacaaaat ggcgagtctc tccgggaaga
     3541 agccgagctg agtctggccc cgttcttggt catctcaaag ccatgccaca tggaagcatt
     3601 cagtcactcc cggtgtgcgt ttggaggaca tccagctacc cacataccag cagccaggcg
     3661 ccagtcgggt catggtgggg ccgcctcccg ctgcctcctt tcctcttcct tctctctctt
     3721 cagagctgcc atcattaaag agccagcttc cattttcaga cattttctct gatactctgc
     3781 tggcctggta agccacacga gtccattctg tcacagagga ttccctcttc cgaacgatag
     3841 cctggggtag tggcagaggc aggacaaggg caggctctcc agcagcctgt gccttggttt
     3901 cctttctgcc tgggaagggg atgttggaca ggcagacaca gtagcaggtc tgctgcctga
     3961 gctccaccat ccaggcatca acagcaacca ccacagtgag ctgcctctgg cctggggtca
     4021 tctgcatcac ctccagaagc agtgggcata acgttgtgag tccttggaag atgcggggac
     4081 agcatgagag caggagttta gctgatgtca ggaaaggcct aggggaagcc agcagcgctc
     4141 ggagtgtgag catctttccc tggaacgaga gaaagaggag cctaacttgc tatggaagca
     4201 cttaggtttc caggcctgga gatctgttcc aggtctgttt ttttaagtct aggaaagtct
     4261 tgctgacagc tgtttgtagg aaggaaggtg gatcggggct gctgcggctg ctctgtgtga
     4321 actgtttgac ttgatgctgc tacctctcgt ggtgacattg ttataacacc ctggagagac
     4381 ccgagtgctc atggatgtcc cttagctgcc ttagtcggga gaaccatcat catccactcg
     4441 gtggacccaa accgccatca gccatgtgac ttcctaatca tgggtttctt ttggttgaca
     4501 agttctggct caggtgtaaa agccacacga agaaatgagc tgtaagcggt ggcatcactt
     4561 tggatactta gtttaaaagt atatgtgtga ttttctatgt tccaaaacta tcatgagttt
     4621 tttggcccga gcaagtgtag acctcccctt cctttgtgcc cagactgtga ctagtgagct
     4681 gggacagtct ctgtccagag accacacaca cacagtgagc tctcttcctc tcctggctga
     4741 agaggctcta catcattcga tcacagtttg ctccccctca gactcgctga aacatctagt
     4801 ccttctctga aggtcttagg tgtcgggggc caccccgtgt tgcccgttcc tccctacctg
     4861 tcaggaccac tgccatattg atgcaagata tttgacaatt gatacaggac agacacgagc
     4921 cacacagagc cggtgtcttc tgtagaatag tggtgggcac tgctgttcag ggcctgtggg
     4981 atgaacgtga ccctgcttcc gatcccagaa tggcctctgg aatgtgggtt tgtctggtct
     5041 ttactcatgg gagatgtgcg ttccatctga gcagccaccc tggcttgctc tcaggtagca
     5101 catactgcaa caggtgggtt tcctagaaat cagtggacat tcaagtctct gttagccaaa
     5161 cagcccaagc atgtcacttg aggacagatt taataagcat agccttgccc accttggatt
     5221 aggagccggc ctgtcagtgg gaaaggtttt tgatgccttc ctggccctcc tcacacttct
     5281 ggaactcgag tcttttctca ctgcttgcat tctctgggaa cacccctctc ttcccggact
     5341 ggtttctcct ctgtttttcc tgccaccttc tgtgtaggga aactgatgac tgcttcaccc
     5401 tgccctggcc tcagtgcact tgagagacat aatggtgagg accctgacgt atgagggagc
     5461 cagtgacttg tcactgaaac ttactcttcc cacacgatga cgcttgtgca gcctcctgtg
     5521 agctatgccc tgggctggag ggtggaggct gctctcgccc gccctgagca atgcacttgt
     5581 ggagcctcat gcccatgttt gcactggtca tctcttctat gacaagggtc gctctcagac
     5641 acgtcttgaa tgtggccagc taatgtcatg acttgctggt aatgtcagct ggtggtgtga
     5701 gtggagttgg ggggctggga ctcaagaaca cgttaattga agccttggct ctgagggaaa
     5761 agatggaggg ttcctaagtg gccagttgct tcccgatgac catcagggaa gggttgctgt
     5821 agagaaaggt gaagagctgg agagacggaa tgtgcaagtg ccaagtgtgt ttggttgcat
     5881 aacacatgca acttgggaag ctttgtcagg aggctccgga ctttcccact cccagcccca
     5941 tgttgggtct ccttaaccat gaaaacctgt cttcccgata gagggaaagt gaaccatctc
     6001 tctccaaaag acagttgcgg tttctcattg tcggtgatgg agcagacttt taaattactc
     6061 actaatgttg gatgaaaatg tggtattcac taagttccca gcctgcccag actctgcaga
     6121 gtctgtggtt tatgatcgtc cagaaaagga cagttcctaa ctccactttt ccttgacctg
     6181 ctcagctcag cagacctgct ccttagtggg gaggcagggg ttctccttgt atcctgtgta
     6241 gtaggtgcag tgtctgtcct gcctctccac ttgtcctggg ttgtagtgag tagcagacac
     6301 actgtctcct gtgtgtctcc caccactggg ccgctcgatg tccctgaaga tcttttgatg
     6361 taatttaatc gtatgggtgt tttaccttca cacaggcctt tgcaccacat gtcccccatg
     6421 cttcaggaga tcagaaggaa tccgatcctc ctggagctgg agttacaaat agctgtgtgc
     6481 ttccatgtgg atgctgggga tttaattgaa cctgggtgta gctggtcttt gctactttgt
     6541 gggttatttt ctcacccttt gctccctctc ccagtttcac tccttgacac tagataggac
     6601 aaaaacgggg ggcgagaggg agagggagag agagagagag atcgagatca agagagacac
     6661 ccctgtatct aaattccttt cttctttgaa cactacgaag aaaccacaac caaccacaca
     6721 caactcccaa acaaccaaca accacccatc ctgctttttg ggagcctagc atttatagac
     6781 tttctgaaaa gttcctagaa ttccaaaact tgacacaatg ccagagactg tctgcagctg
     6841 gcaaaaccac acttctgcta gagcactagg caaatcatgt cagctgctgc cgacactcca
     6901 aagcagcccc acatccccac acctggggac taaaatgata acatattctt atatttgttt
     6961 tttaaagaaa ccagaattcc agagttctca ccacgattcc ccacttctgt tttaagtcat
     7021 taattgtgct gtgtatatca ggaaattata aacagacatt ttcaaggaac tgagtgttca
     7081 ggttagtgcc aaggtgttga ggagacctct cctgctcggt cctaatcttt atcagttttg
     7141 atggtatcca cagcttttcg tttcctgtgg aagtataaac aattccccat ccccaatgca
     7201 aaacttttct gttctaatat taacacatct ttattctata ttgtaaaaaa ttcagttttt
     7261 cctctcggcc cctgctccag gaatgaccac tccaagactc cttattatta aatgccttgg
     7321 ccagaagcct tgcctcatcc atgactggct cgtaacctat tcaacccatt caaactcttc
     7381 tatgtcatcc tcatggttac ttctcctcag tcccggcttg cttcatcctt ctcactacct
     7441 cctccttgtc tcactatctc ccagaatcct ctcttcctgc cattgtccca cctcctattt
     7501 cttgcttcag ctcattggcc attggtggtt tttattgaca agagatgcta ataagagatt
     7561 ctacagtaca taggctgatg taattccaca gttttttttc taaaacccag agtatctcaa
     7621 ctgctgctgt tcctttctca ttaatattca gaaaatttaa agttagcaga gagtaattgt
     7681 ctcttttttt ctctttgctt tataagcatt tcctttaggt aaaatgagat ctttctacaa
     7741 ctgcctgtcc tgtaggattg catgaatatt aatctgagca ctgcacagtt gtagaagagc
     7801 ccttccccat agactgatgg gtcggtcaga gtccttcccc atagactgat gggtcggtca
     7861 gagcccttcc ccatagactg atgggtagat cagccacatc aggtcttagc tttcctgtta
     7921 gaccttctgg tcccacacat ttcacccatt gcacactttg ttttatctga gataaatttc
     7981 cgattcctag aaactgtata aaccttccaa agtggccatt ctgaattcca agattttttt
     8041 gtgaactgat agttacatca gcgcctgtat ccacaaattt gtctacttca gtacctttta
     8101 tgtgaaattt taatatttgc ctctgatcat taacaactgt ttgccggaac acatgctttt
     8161 cagtcctttc agaccctcct gttctctcta ctggagcagc tttgtctctg ggacagggaa
     8221 acagtagcag ccgaacaagc ctctatcacc tgcattaatt tgcatctctc tccctctccc
     8281 tcacacacac acatgccata actttaattt ctcctttgaa atcttcatgt atgattcctg
     8341 ggtgcacagt gaatccttgg ggaatcaatc catctattct caagatcatc ccactgtctc
     8401 tgcaggcaaa ggagcacgaa ctctctcgaa gttttggata tagagtaatg cttttctgtg
     8461 gccgagtcta gagcagcact gcctgcagag gtgttagatc tccagtgctg agtgcaggtt
     8521 ttagtctgcg tgttatggct tgactggaac agctcacagt ctttgcttgg tggggagcta
     8581 agctaggccc ccaaggctgt ttccttgaat atctcttttg gatctgcatt ccggaatcca
     8641 gtggctgcct ttgccaaagt gcatacacat tgcaggaagc ctacgagtcc tgtttcagat
     8701 tcaggaaaac cattgcctct agaaatgact gattcactct tctgctgcaa agtactgtct
     8761 ttctcccagt taacgcactg ggtgggtatt ttgaggtcag agatttttag ctctagcttg
     8821 gcctgttcta ttagcgtggt acacattaga accaatatcg atcacatccc ttatccattc
     8881 tgccatagac gcttgctcga gcctttaacg tttcgataac ttttttgcat ttggttattc
     8941 gccttttcaa caactaaggc ctctatcaac acctgccttc atcttgatct ggtacggctt
     9001 ccttcacagc tgagactaat ctttgtaatc agtgaaggct tctcagaagc cctgtataat
     9061 ctttataaat gaggtggtct ttttcctggg cccctcaaat ttgtcccaag ttttaaagcc
     9121 accaagcaac attgttcagt agtaatatta tgtgattgaa cctgttcttg tatatcagag
     9181 ccctgccctt cacccagcaa tggtggagct gatgggattc ggctcagaga gctgccgctg
     9241 gagctgctgc ttgtggtgct aaggactaga gaatgatggg aaaatcctac actgagtggc
     9301 tgaagcaagg caagctccaa caactttgtt ttctccttca gctcgtttgt acccaaaagt
     9361 ggtcaccaca aaacactttt gaaaaacatg ttaaaatcac tacacaaact gaaattacaa
     9421 caggattact gcttgcatat ccttcttttg gaactttgac ctaactctac attcctcatc
     9481 tgtctttctc tccacaagtt cactgccacc ccaaagcaat aattctcttg tcatagacta
     9541 acaaagctta agcagaccaa gtagagagta gtatgtttct cccagtgctt agatggtgac
     9601 aatcttgttt gctaaggaac aggtcatctg tcttgcatct tgtttttgaa gtcttggtca
     9661 gctagactgg ctagtcctct ctctctgttc ttacatacac agtgaagtcc cctgctcttc
     9721 gttcatgacc ttccttctca tggtgcacac agaaaccagt gccacacctc tggatcacaa
     9781 gatcaaggaa cttagaccca acctggaatg aatgtcttgt ttgataatta acttgtcccc
     9841 agcctgtttt tcttgctcct acaatcgcgc gcttgcaacg ctgagggctc actggcgccc
     9901 tcacctgtgc cagtctatga gaaagggcca cactctgttc tcgattcgaa ctttgaactt
     9961 tctatcaaat actcttaact tccccaaaat tttaaattaa actaacttcc ccaaactact
    10021 ttaaattggg aattccataa aatcattaat tcatgtaagc agcattatct caggaatgtt
    10081 catcttcagg ttgatcttca ggaaacttgc cccacagagt gggctaccac ccagaagtgg
    10141 tcactccagg ctacggaggc agtgagtgtc tcactatgtc aagtacatga gggatatagc
    10201 agtgacaaac atgagaaggc acagcagtag caagaagcat ccctgggacg aagcccctag
    10261 ggctggacat ctccagggca cacccaccca tcacagccaa gcagagctgc aggctccagg
    10321 gcacacccac ccatcacagc caagcagagc tgcaggctcc agggcacacc cacccatcac
    10381 tgccaagcag agctgcaggc tccagggcac acccacccat cactgccaag cagagctgca
    10441 ggctccaggg cacacccacc catcacagcc aagcagagct gcaggctggc tccaggaagc
    10501 tcacttgccc actgcagctc cccctgcatg gcgttcaccg tcccttggag gaaagaatcc
    10561 cactcggagc tcctcactgt gccacagcta aggcggccgc tgatgctccc aggagaggga
    10621 ctggatggct atgttctgac gtgacaagtt gagggactga gaatccttaa gctcccatgc
    10681 tcacctgtat tgatctccat tcaccgtaaa cagaaaaaca cgtccagagg ccaaatagag
    10741 gcaaactttc tactgtagat aaaaatgact cttggtatct gcctccaaca gccttggttt
    10801 ttcaaggcag ggtctcactg tgtagagcaa gctagccttg accttgctgt aactttcttg
    10861 cctcccagcc gctgggtcaa gcatgcacaa ccatacctgg ctttgccctt ggtctctctt
    10921 aggcggactc agttcttctt cagtaccttt ttctttgtgg ccagaaagga gaattcgtga
    10981 agtaatgcgg tttggagtgc atgagaggaa tgccgtcttc tcattcacag gcataaattg
    11041 tcttaggttc tgtgctcagt gggaatggat tggtttgcgc gcgggggagc gggggggggg
    11101 ggccgaagag aagaggaaca gcgtccccca ctccagagct aacccaccct gaggatcaga
    11161 ggatagagtt ttccactaag cagggtttcc tgagcaacag ggaggccctc catccctcac
    11221 acccacaaac cttttctaag cccagaagca gtcttgacgt ggacatcgct ttcagggttt
    11281 atgggtgact tcagcgtgca tggtttcact tgtgcaacag tgcgaggcac cagcctgagt
    11341 gtgcaacctc tccttttgtg aaagaaacca aaattcttcc tgtgtctcct cctctctggt
    11401 ctctgtgtgt tgattggtta gaagtggctg atggtgtccc ttggagagca gagcaacctt
    11461 cccatggaag gggacacttt gaggtgctgg tgtgtctccc tcattttcta tgttcttctc
    11521 tctagtaggt ctcaagtaga gacagatatt tttgttttag agattccaaa gtctatattt
    11581 ttagtgtaag aaatgtaccc tctccacact tcatgtcgta aatagaactg ggaataagat
    11641 gtgcaattgt gatcaccctg taacaatgta tggtagacat gaggccccgg ggcaggtgac
    11701 ttggtgtgtc tctgagccct cctctctgcc tgcccacaca catgtgtgct cagctctttg
    11761 agtgacaaat aaagcccatg ttataaactg attaaaaaaa aaaaaaaaaa a
//
