LOCUS       NM_001359211            7588 bp    mRNA    linear   ROD 04-FEB-2025
DEFINITION  Mus musculus solute carrier family 4 (anion exchanger), member 4
            (Slc4a4), transcript variant 4, mRNA.
ACCESSION   NM_001359211 XM_017320996
VERSION     NM_001359211.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 7588)
  AUTHORS   Brady,C.T., Marshall,A., Eagler,L.A., Pon,T.M., Duffey,M.E.,
            Weil,B.R., Lang,J.K. and Parker,M.D.
  TITLE     Left Ventricular Systolic Dysfunction in NBCe1-B/C-Knockout Mice
  JOURNAL   Int J Mol Sci 25 (17), 9610 (2024)
   PUBMED   39273556
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7588)
  AUTHORS   Ye,Q., Jo,J., Wang,C.Y., Oh,H., Zhan,J., Choy,T.J., Kim,K.I.,
            D'Alessandro,A., Reshetnyak,Y.K., Jung,S.Y., Chen,Z., Marrelli,S.P.
            and Lee,H.K.
  TITLE     Astrocytic Slc4a4 regulates blood-brain barrier integrity in
            healthy and stroke brains via a CCL2-CCR2 pathway and NO
            dysregulation
  JOURNAL   Cell Rep 43 (5), 114193 (2024)
   PUBMED   38709635
  REMARK    GeneRIF: Astrocytic Slc4a4 regulates blood-brain barrier integrity
            in healthy and stroke brains via a CCL2-CCR2 pathway and NO
            dysregulation.
REFERENCE   3  (bases 1 to 7588)
  AUTHORS   Everaerts,K., Thapaliya,P., Pape,N., Durry,S., Eitelmann,S.,
            Roussa,E., Ullah,G. and Rose,C.R.
  TITLE     Inward Operation of Sodium-Bicarbonate Cotransporter 1 Promotes
            Astrocytic Na+ Loading and Loss of ATP in Mouse Neocortex during
            Brief Chemical Ischemia
  JOURNAL   Cells 12 (23), 2675 (2023)
   PUBMED   38067105
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 7588)
  AUTHORS   Oh,Y., Kasu,M., Bottoms,C.J., Douglas,J.C., Sekulovski,N.,
            Hayashi,K. and MacLean Ii,J.A.
  TITLE     Rhox8 homeobox gene ablation leads to rete testis abnormality and
            male subfertility in micedagger
  JOURNAL   Biol Reprod 109 (4), 520-532 (2023)
   PUBMED   37471646
REFERENCE   5  (bases 1 to 7588)
  AUTHORS   Pethe,A., Hamze,M., Giannaki,M., Heimrich,B., Medina,I.,
            Hartmann,A.M. and Roussa,E.
  TITLE     K+/Cl- cotransporter 2 (KCC2) and Na+/HCO3- cotransporter 1 (NBCe1)
            interaction modulates profile of KCC2 phosphorylation
  JOURNAL   Front Cell Neurosci 17, 1253424 (2023)
   PUBMED   37881493
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 7588)
  AUTHORS   Alvarez,B.V., Loiselle,F.B., Supuran,C.T., Schwartz,G.J. and
            Casey,J.R.
  TITLE     Direct extracellular interaction between carbonic anhydrase IV and
            the human NBC1 sodium/bicarbonate co-transporter
  JOURNAL   Biochemistry 42 (42), 12321-12329 (2003)
   PUBMED   14567693
REFERENCE   7  (bases 1 to 7588)
  AUTHORS   Kim,Y.B., Yang,B.H., Piao,Z.G., Oh,S.B., Kim,J.S. and Park,K.
  TITLE     Expression of Na+/HCO3- cotransporter and its role in pH regulation
            in mouse parotid acinar cells
  JOURNAL   Biochem Biophys Res Commun 304 (4), 593-598 (2003)
   PUBMED   12727194
  REMARK    GeneRIF: These results suggest that the pancreatic
            sodium-bicarbonate cotransporter contributes to pH(i) regulation in
            the parotid acinar cells.
REFERENCE   8  (bases 1 to 7588)
  AUTHORS   Bachmann,O., Rossmann,H., Berger,U.V., Colledge,W.H., Ratcliff,R.,
            Evans,M.J., Gregor,M. and Seidler,U.
  TITLE     cAMP-mediated regulation of murine intestinal/pancreatic Na+/HCO3-
            cotransporter subtype pNBC1
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 284 (1), G37-G45 (2003)
   PUBMED   12388213
  REMARK    GeneRIF: existence of an intracellular HCO(3)(-) concentration- and
            cell volume-independent activation of colonic NBC by an increase in
            intracellular cAMP.
REFERENCE   9  (bases 1 to 7588)
  AUTHORS   Praetorius,J., Hager,H., Nielsen,S., Aalkjaer,C., Friis,U.G.,
            Ainsworth,M.A. and Johansen,T.
  TITLE     Molecular and functional evidence for electrogenic and
            electroneutral Na(+)-HCO(3)(-) cotransporters in murine duodenum
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 280 (3), G332-G343 (2001)
   PUBMED   11171615
REFERENCE   10 (bases 1 to 7588)
  AUTHORS   Abuladze,N., Lee,I., Newman,D., Hwang,J., Boorer,K., Pushkin,A. and
            Kurtz,I.
  TITLE     Molecular cloning, chromosomal localization, tissue distribution,
            and functional expression of the human pancreatic sodium
            bicarbonate cotransporter
  JOURNAL   J Biol Chem 273 (28), 17689-17695 (1998)
   PUBMED   9651366
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC162690.2, AC140492.3 and AC165240.6.
            
            On Dec 29, 2017 this sequence version replaced XM_017320996.1.
            
            Transcript Variant: This variant (4) represents the longest
            transcript and encodes the longest isoform (d).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660817.264094.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849380, SAMN00849381
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-65                AC162690.2         80836-80900
            66-139              AC162690.2         128325-128398
            140-319             AC162690.2         148354-148533
            320-455             AC140492.3         92323-92458         c
            456-616             AC140492.3         82140-82300         c
            617-796             AC140492.3         74359-74538         c
            797-892             AC140492.3         62707-62802         c
            893-969             AC140492.3         36033-36109         c
            970-1127            AC165240.6         169308-169465       c
            1128-1215           AC165240.6         162141-162228       c
            1216-1370           AC165240.6         159342-159496       c
            1371-1484           AC165240.6         158597-158710       c
            1485-1659           AC165240.6         156047-156221       c
            1660-1793           AC165240.6         142434-142567       c
            1794-2065           AC165240.6         135265-135536       c
            2066-2136           AC165240.6         121046-121116       c
            2137-2328           AC165240.6         112023-112214       c
            2329-2442           AC165240.6         94105-94218         c
            2443-2604           AC165240.6         92051-92212         c
            2605-2783           AC165240.6         77215-77393         c
            2784-2856           AC165240.6         76074-76146         c
            2857-2925           AC165240.6         68603-68671         c
            2926-3099           AC165240.6         65894-66067         c
            3100-3261           AC165240.6         62880-63041         c
            3262-3358           AC165240.6         59007-59103         c
            3359-3438           AC165240.6         56991-57070         c
            3439-7588           AC165240.6         52211-56360         c
FEATURES             Location/Qualifiers
     source          1..7588
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="5"
                     /map="5 44.1 cM"
     gene            1..7588
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /note="solute carrier family 4 (anion exchanger), member
                     4"
                     /db_xref="GeneID:54403"
                     /db_xref="MGI:MGI:1927555"
     exon            1..65
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            66..139
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             67..3402
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /note="isoform d is encoded by transcript variant 4;
                     pancreas sodium bicarbonate cotransporter; electrogenic
                     sodium bicarbonate cotransporter 1; solute carrier family
                     4, sodium bicarbonate cotransporter, member 4; solute
                     carrier family 4 member 4; Na(+)/HCO3(-) cotransporter"
                     /codon_start=1
                     /product="electrogenic sodium bicarbonate cotransporter 1
                     isoform d"
                     /protein_id="NP_001346140.1"
                     /db_xref="GeneID:54403"
                     /db_xref="MGI:MGI:1927555"
                     /translation="MEDEAVLDRGASFLKHVCDEEEVEGHHTIYIGVHVPKSYRRRRR
                     HKRKAGHKEKKEKERISENYSDKSDVENADESSSSILKPLISPAAERIRFILGEEDDS
                     PAPPQLFTELDELLAVDGQEMEWKETARWIKFEEKVEQGGERWSKPHVATLSLHSLFE
                     LRTCMEKGSIMLDREASSLPQLVEMIADHQIETGLLKPDLKDKVTYTLLRKHRHQTKK
                     SNLRSLADIGKTVSSASRMFSNPDNARSPLENSVPCLANRTLDDDPRVRRTPSVGWLS
                     SPAMTHRNLTSSSLNDISDKPEKDQLKNKFMKKLPRDAEASNVLVGEVDFLDTPFIAF
                     VRLQQAVMLGALTEVPVPTRFLFILLGPKGKAKSYHEIGRAIATLMSDEVFHDIAYKA
                     KDRHDLIAGIDEFLDEVIVLPPGEWDPTIRIEPPKSLPSSDKRKNMYSGGENVQMNGD
                     TPHDGGHGGGGHGDCEELQRTGRFCGGLIKDIKRKAPFFASDFYDALNIQALSAILFI
                     YLATVTNAITFGGLLGDATDNMQGVLESFLGTAVSGAIFCLFAGQPLTILSSTGPVLV
                     FERLLFNFSKDHNFDYLEFRLWIGLWSAFMCLVLVATDASFLVQYFTRFTEEGFSSLI
                     SFIFIYDAFKKMIKLADYYPINSDFKVGYNTHFSCACLPPDPVNLSVSNDTTLAPEDL
                     PTISSTDMYHNVTFDWAYLSKKECVKYGGKLVGNNCDFVPDITLMSFILFLGTYTSSM
                     AMKKFKTSRYFPTTARKLISDFAIILSILIFCVIDALVGVDTPKLIVPSEFKPTSPNR
                     GWFVPPFGGNPWWVCLAAAIPALLVTILIFMDQQITAVIVNRKEHKLKKGAGYHLDLF
                     WVAILMVVCSFMALPWYVAATVISIAHIDSLKMETETSAPGEQPKFLGVREQRVTGTL
                     VFILTGLSVFMAPILKFIPMPVLYGVFLYMGVASLNGVQFMDRLKLLLMPLKHQPDFI
                     YLRHVPLRRVHLFTFLQVLCLALLWILKSTVAAIIFPVMILALVAVRKGMDYLFSQHD
                     LSFLDDVIPEKDKKKKEDEKKKKKKKGSLDSDNDDSDCPYSEKVPSIKIPMDIMEQQP
                     FLSDNKPLDRERSSTFLERHTSC"
     exon            140..319
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            320..455
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            456..616
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            617..796
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            797..892
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            893..969
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            970..1127
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1215
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1216..1370
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1371..1484
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1485..1659
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1660..1793
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..2065
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2066..2136
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..2328
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2329..2442
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2443..2604
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2605..2783
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2784..2856
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2857..2925
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2926..3099
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3100..3261
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3262..3358
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3359..3438
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     exon            3439..7588
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7563..7568
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /note="hexamer: AATAAA"
     polyA_site      7585
                     /gene="Slc4a4"
                     /gene_synonym="NBC; NBC1"
                     /note="major polyA site"
ORIGIN      
        1 gcggccgctg cagcctcaca ctcggtcgcc aagctggagg agcgacggaa gcccgacccc
       61 aggaggatgg aggatgaagc tgtcctggac agaggggctt ccttccttaa acatgtgtgt
      121 gatgaagaag aagtagaagg tcaccacacg atctacattg gggtccatgt gcccaagagc
      181 taccggagaa ggagacgcca caagaggaag gctgggcaca aggaaaagaa ggaaaaggag
      241 agaatctccg agaactactc cgacaaatct gatgtggaga atgcggatga gtccagcagc
      301 agtatcctca aacccctcat ctccccggcc gcagaacgca tccgattcat cttgggagag
      361 gaagatgaca gcccggcacc tcctcagctc ttcacggaac tcgatgagct tctggctgtg
      421 gatggacagg agatggaatg gaaggagaca gcgaggtgga ttaagtttga agagaaagtg
      481 gagcagggtg gggagcgatg gagcaaaccc catgtggcca ccttgtccct gcacagcctg
      541 tttgagctga ggacatgtat ggagaaagga tccatcatgc ttgaccggga ggcatcttct
      601 ctcccacagc tggtggagat gattgcagac caccagatcg agacaggcct actgaagcct
      661 gacctgaagg ataaggtcac ctatactctg ctccggaaac atcgacatca aaccaagaaa
      721 tccaaccttc ggtccctggc tgacattggg aagactgtct ccagtgcaag taggatgttt
      781 agcaaccctg ataatgcccg aagcccgctt gagaactcag tgccctgcct agcaaatcgc
      841 accttggacg atgacccccg agtgcggcgc actcccagcg tgggatggct cagcagccca
      901 gccatgaccc acaggaatct gacatcctcc agtctcaatg acatttctga taaaccagag
      961 aaggatcagc tgaagaataa attcatgaaa aaactgcccc gagatgcgga agcttccaat
     1021 gtgcttgttg gggaggttga cttcttggac actcccttca ttgcctttgt tcgcctacag
     1081 caggctgtca tgctgggtgc cctgactgag gtccctgtgc ccacaaggtt cttgttcatt
     1141 ctcttaggtc caaaggggaa agccaagtcc taccatgaga ttggaagagc tatcgccacc
     1201 ttgatgtctg acgaggtgtt ccacgacatc gcttacaaag cgaaagacag acacgacctg
     1261 attgctggca ttgatgagtt cttagatgaa gtcattgtcc ttccacctgg ggaatgggac
     1321 cccacaatcc gaatagagcc tccaaagagt ctcccatcat ctgacaaaag aaagaatatg
     1381 tactcaggtg gagagaatgt tcagatgaat ggggacacac ctcatgatgg aggccacgga
     1441 ggaggaggac atggtgactg tgaagaacta cagagaactg gccggttctg cggtggatta
     1501 attaaggaca taaagaggaa agcaccattt tttgccagtg acttttatga tgctttaaac
     1561 attcaggctc tctctgcgat tctcttcatt tatctggcaa ccgtaaccaa cgccatcact
     1621 tttggaggcc tgctcgggga tgccaccgac aacatgcagg gtgtgttgga gagtttcctg
     1681 ggcactgctg tctccggagc catcttctgc ctttttgcgg gtcaaccgct taccatcttg
     1741 agcagcacgg gaccagtgtt ggtgtttgag aggcttcttt ttaacttcag caaggaccat
     1801 aattttgact acttggagtt tcgtctttgg attggcctgt ggtcagcctt catgtgtctt
     1861 gtcctggtgg ccactgatgc gagcttcctg gttcagtact tcacccgttt cacagaagaa
     1921 ggtttctcct ctctcatcag cttcatcttc atctatgatg ctttcaagaa gatgatcaag
     1981 ctagcagatt actatcccat caactctgac ttcaaagtgg gttacaatac tcacttctct
     2041 tgtgcttgcc tgccacccga cccagttaat ctctcagtat ctaatgatac cacactagcc
     2101 ccagaggacc tgccgaccat ttcttctact gacatgtacc ataatgtcac ctttgactgg
     2161 gcctatttgt caaagaagga gtgtgtgaag tatggaggga agctcgtggg aaacaactgt
     2221 gacttcgtgc ctgatatcac actcatgtcc ttcattctct tcctgggcac ttacacctcg
     2281 tctatggcta tgaagaaatt caaaaccagt cgctattttc caaccacagc aagaaaactg
     2341 atcagtgatt ttgccattat cctgtccatt ctcatattct gtgtaataga tgccctagtc
     2401 ggcgtggaca ctccgaagct cattgtacca agtgagttca agccaacaag tcctaacagg
     2461 ggttggtttg tcccgccatt tggaggaaac ccttggtggg tgtgccttgc tgctgccatc
     2521 ccggctttgc tagtcaccat cctgattttc atggaccagc agatcaccgc tgtgattgtg
     2581 aacaggaaag agcataaact caagaaagga gctgggtatc acctggatct gttttgggtc
     2641 gccatcctca tggtggtatg ctccttcatg gctcttccct ggtatgtggc tgctactgtt
     2701 atctccattg cccacattga cagtctgaag atggagacgg agacatctgc gcctggagaa
     2761 caaccaaaat ttctgggagt aagggaacaa cgagtcactg gaacccttgt gtttattctg
     2821 actggcctgt cagtcttcat ggctcccatc ctgaagttta tcccgatgcc tgtgctgtat
     2881 ggtgtgttcc tgtatatggg ggtggcctca cttaacggtg tgcagttcat ggaccgtctc
     2941 aagctgctgc tgatgccctt gaagcatcag cccgacttca tctacctgcg ccacgtcccc
     3001 ctccgccgag tccacctgtt caccttcctg caggtgttgt gcctggctct gctctggatc
     3061 ctcaagtcaa cagtggctgc aattattttt cctgttatga tcctggccct ggtagcagtc
     3121 agaaaaggta tggattacct cttctcccag cacgacctca gcttccttga tgatgtcatt
     3181 ccagaaaagg acaagaaaaa gaaggaggac gagaagaaaa agaaaaagaa gaaaggaagt
     3241 ttggatagcg acaatgacga ttctgactgc ccatactcag aaaaggtccc cagtattaaa
     3301 attccaatgg acatcatgga acagcaacct ttcctaagtg ataacaaacc cttggacaga
     3361 gaaagatcct caacattcct cgaacgccac acatcatgct gataaaattc ctttccttga
     3421 gtcgctgggt ttacccagtc ctccaagaac tccagtgaaa gtcgtgcctc aaattagaat
     3481 cgaacgtgag cctgaagaca acgattatct ttggaggagc aagggaacag aaaccacgtt
     3541 gtaacctgtt tgtccttctc aaaactgaca cttctgttgt taactgttct aatttttttt
     3601 tgtctatgta ttttaaaatc ttttgtttgg tcactggctg aataatagtc actctctctg
     3661 cttctctctt gcataggtaa aaatcaagac aatagtgtcc cttgccttta aaaaaaaaaa
     3721 aaagaagcat ttgagggtca cacgcttgtt ttcatacatt cagatgtgtc ctccgacatg
     3781 taaatccctt tgtcactcaa gacacacaga aaccgccctt gtcaagcaga gatcaaatgt
     3841 gacgagaatt tttgtatcac cttttatgaa actattgaaa gaacttacaa cctaagcttg
     3901 gagcttggct tggttctgtc tggcagagca gcccttgacc tccagacaga gtcccttcct
     3961 gcttacagaa tccccaagac tggaaaagtg ctgctaccaa cctgcccttg ctcgccaacc
     4021 ctgatcttag agttatcaaa agcagaaaac actaaaggta cttcgctccc tgtagagaaa
     4081 cctttgccat cattgtagca agtgctggaa tgtccctttt cctatgcaac tttttttaac
     4141 cctttaatga acttatctgt tgagtacatc gaagaatatt tttcttccta gattttgttg
     4201 tttaaattat ggggcctaac ctgcaactta ttttttgtca attttttaaa ctttttttta
     4261 attactgtaa agaaaatgaa tttttttcct gcagcaggaa acatagtttt gagtagttct
     4321 acctcttacc tgtagctgcc aggctttctg taaaaattgt attgtatata atgtgatttt
     4381 tacacacatg cgtgcacgtg agcacgtaca cacacacaca cacacacaca cacacacaca
     4441 cacacacaca ccaatctagg gtaagccaga aggctagaac aaattaaaag aaaaacaaaa
     4501 caccatgctt ctaagaatct gttgggttgt tgggttgttc ccctctcccc ttttttcaaa
     4561 ataaatgtag ctgttttgtg aaggaatctg gggagcagag agaaatgtgt gcagggggaa
     4621 taacttctgc tgtggtaatg actgtgctgg gaacagatgc tggcatgtta catgtcgaca
     4681 tcctatgcag tattgttagg gatttcctca ttttaataat ttgtgtcttt gtgtaagaac
     4741 tctgtgtgtg gttgttgcac acctgtgcaa agccagataa gtgacagtag ccagagggca
     4801 aagctgtcac gctccttgtg tccattttca taaaatccaa aaccactcat aagaactttg
     4861 tcaggggcca agaaatgtat cgtggacatt caggtgaaga tatcttacta ctcagacatc
     4921 agtcagcatc ccagatacaa gagtctagag ctacaataca aaagccatat gaatattatc
     4981 agctctgtgg tgttacacat ctgtaacatg gtataggaat gcacatagat ctgcaaggaa
     5041 agtttaaagt gttcatttac atgccctcat aggataggat tattagacca catgtatgtg
     5101 tgtgtatctg tgtgcttcaa tagaccgagt tgaaaattag actttcctta gccaaattga
     5161 tgtggcattt gattccaata tttttagtaa gctgcgttat atgcggatta tatattgaga
     5221 taatagtttg gatgtggaat tcatattttt ggatttgtat agcaacagtt gtgatacaca
     5281 ttcactggga gagattgctg ggagcattgg agaagcagaa gggaaccacc tactatgact
     5341 gggtctgtgg ttggctctta cagactccca ctttcttaca ggaacgaaaa tgtaaccccg
     5401 ctctcctgaa atcttgatca gagttgtatt atcctgctag atgttacttc catggcattt
     5461 gttcatcttg aaactttaga ttttagagta tttaggcatg aacgacccca agggtttttt
     5521 gttgttgtta ttgaagtcat agcagcaaat tacatgtcat ttgttaccat ctcaaatgat
     5581 acatttttac attgcatttg ttctgagggt tatcctgaaa atggttgttg tgcattttct
     5641 ggttttctat taaaccgtgt aaggaatctt acctttttag ggataaacac atcagtaaaa
     5701 tgttaaaagc atgcattgta aatgcagtcc aatcttgatg ttgaatcaaa gacacttggt
     5761 ttacttttat aaaatctctg gatatatata atatatataa ataattttta attatgaatc
     5821 aagtctatat agggactcat gaccctcctc ttacttttca gagagctcct ggagttagtc
     5881 acgtcagtcc ttgagtagat cctgaagtta tctcacccca tgtatggtcc ctagtctgtg
     5941 actggggtgg atgttagtat tcatggactg aacatagagc catacctgac atttgcttga
     6001 ggagtaggta acaacgagtg ttctcattac cacctgagtt cccacactaa caagcagaac
     6061 tccaaatctc catggatgcc ccgcagtatc tgaatctggc tgaatttgct cattggccga
     6121 taactaagca gatttcatgg tggtttgttt ctatgcaaac tggacacaaa tacagcaatg
     6181 aattctttat aatgcctttc tacccttcct ccctggatgt gaatttggag ataagtgttt
     6241 cttgaagcaa agactcagag ttgttggcca ggagtactgt ggtagtgcaa actggctggg
     6301 agagagatgt gcaggctgag cctgcttgtc gaagcatcaa ggcctccagt ttgccgagat
     6361 gctgagatgc tggtcctgtc ctactgtgtg cagaaactac taatggtttt gtatatcaga
     6421 ggcggtattg tttcagcctc tcctgataaa gccgtggggt ggggctggga agtactcttt
     6481 tgagaagaca caaaccagac cttccttctt gtcccccacc taagagaaag ggctgtctgt
     6541 tgtcagtaat ctggcttatg tgcattttgg gcacatgtaa caacacacac agagtaaatc
     6601 ctttgtcagt cacgtgttgc tgtttaatat agcaaatgat tgtcttccat gtttttgttt
     6661 cttttatttt aactgtgttg aagtacttga aatgtattga ctgctgagaa tctttaaaaa
     6721 aaaaaaacat ttgagttata ctacagaggt tggcattgat caggaatggg acaaagtttt
     6781 cttcaatctt cttttttttt ccataccact tcacgatttt ggtgcaagga cctgagattt
     6841 tcttctttat attctgtgat gtttcccatc tgctctcaca gcaggagcat gaagcccagt
     6901 tggtacaaag cgactggaga cagaagacag cccttgggga gtcttcagat gagtttatat
     6961 agcttcctgg tagcctgaca tgaataccct tttgtaacat aggtggatgt ggcaggctgg
     7021 gtttgactag agagttggat ctatcttagt cctggctcat ttcactgtgc tctgcggata
     7081 cacacaagaa tcctgaattc tgtttcctag gcaaatactg caactcaggg ctaaagtcac
     7141 ccagtgaatc cttagagcct gaagtaactt tgtcccaggc ctacaatgcg tatcaactgc
     7201 agactcgcct ccgtgcagcc aagttcatgg ctggtacata gccgtacaca ttatgtttca
     7261 caatcagtac gcactttcag gtagtcttat ttttattctc ttaagtcttt attaactttg
     7321 gagaaatgat gcatcttttt attttaaatg aagtagatca acatggtgga acaaaatgat
     7381 aaagaacaga aaacatttca atatattact aataatgttt ttccaatatg aaacctaaaa
     7441 ttcctataac atagtatttt acagttttat gaagctttct attgtgactt ttatggaatt
     7501 aagagatgaa gaagatgaga tattttagca tttatatttt tcaaaattaa atgtatactt
     7561 aaaataaagt aactttatgc atttatga
//
