LOCUS       NM_021053               2060 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus solute carrier family 46, member 2 (Slc46a2), mRNA.
ACCESSION   NM_021053
VERSION     NM_021053.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Bharadwaj,R., Lusi,C.F., Mashayekh,S., Nagar,A., Subbarao,M.,
            Kane,G.I., Wodzanowski,K.A., Brown,A.R., Okuda,K., Monahan,A.,
            Paik,D., Nandy,A., Anonick,M.V., Goldman,W.E., Kanneganti,T.D.,
            Orzalli,M.H., Grimes,C.L., Atukorale,P.U. and Silverman,N.
  TITLE     Methotrexate suppresses psoriatic skin inflammation by inhibiting
            muropeptide transporter SLC46A2 activity
  JOURNAL   Immunity 56 (5), 998-1012 (2023)
   PUBMED   37116499
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Kim,K.Y., Lee,G., Yoon,M., Cho,E.H., Park,C.S. and Kim,M.G.
  TITLE     Expression Analyses Revealed Thymic Stromal Co-Transporter/Slc46A2
            Is in Stem Cell Populations and Is a Putative Tumor Suppressor
  JOURNAL   Mol Cells 38 (6), 548-561 (2015)
   PUBMED   26013383
  REMARK    GeneRIF: Expression profiling of thymic stromal cotransporter
            (TSCOT), Slc46A2/Ly110, that was shown to be expressed in bipotent
            precursor and cortical thymic epithelial cells.
REFERENCE   3  (bases 1 to 2060)
  AUTHORS   Lee,G., Kim,K.Y., Chang,C.H. and Kim,M.G.
  TITLE     Thymic epithelial requirement for gammadelta T cell development
            revealed in the cell ablation transgenic system with TSCOT promoter
  JOURNAL   Mol Cells 34 (5), 481-493 (2012)
   PUBMED   23178972
  REMARK    GeneRIF: Thymic epithelial requirement for gammadelta T cell
            development revealed in the cell ablation transgenic system with
            TSCOT promoter
REFERENCE   4  (bases 1 to 2060)
  AUTHORS   Ahn,S., Lee,G., Yang,S.J., Lee,D., Lee,S., Shin,H.S., Kim,M.C.,
            Lee,K.N., Palmer,D.C., Theoret,M.R., Jenkinson,E.J., Anderson,G.,
            Restifo,N.P. and Kim,M.G.
  TITLE     TSCOT+ thymic epithelial cell-mediated sensitive CD4 tolerance by
            direct presentation
  JOURNAL   PLoS Biol 6 (8), e191 (2008)
   PUBMED   18684012
  REMARK    GeneRIF: the introduction of a neoantigen into TSCOT-expressing
            cells can efficiently establish complete tolerance and suggest a
            possible application for the deletion of antigen-specific T cells
            by antigen introduction into TSCOT+ cells.
REFERENCE   5  (bases 1 to 2060)
  AUTHORS   Chen,C., Kim,M.G., Soo Lyu,M., Kozak,C.A., Schwartz,R.H. and
            Flomerfelt,F.A.
  TITLE     Characterization of the mouse gene, human promoter and human cDNA
            of TSCOT reveals strong interspecies homology
  JOURNAL   Biochim Biophys Acta 1493 (1-2), 159-169 (2000)
   PUBMED   10978518
REFERENCE   6  (bases 1 to 2060)
  AUTHORS   Kim,M.G., Flomerfelt,F.A., Lee,K.N., Chen,C. and Schwartz,R.H.
  TITLE     A putative 12 transmembrane domain cotransporter expressed in
            thymic cortical epithelial cells
  JOURNAL   J Immunol 164 (6), 3185-3192 (2000)
   PUBMED   10706709
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK040063.1 and AL831738.4.
            
            On Mar 21, 2009 this sequence version replaced NM_021053.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK040063.1, AK017363.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849375, SAMN00849376
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1527              AK040063.1         2-1528
            1528-1528           AL831738.4         103561-103561       c
            1529-2006           AK040063.1         1530-2007
            2007-2007           AL831738.4         103082-103082       c
            2008-2060           AK040063.1         2008-2060
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="4"
                     /map="4 32.81 cM"
     gene            1..2060
                     /gene="Slc46a2"
                     /gene_synonym="5430429N04Rik; Ly110; Tscot; TSO-1C12"
                     /note="solute carrier family 46, member 2"
                     /db_xref="GeneID:30936"
                     /db_xref="MGI:MGI:1353616"
     exon            1..1267
                     /gene="Slc46a2"
                     /gene_synonym="5430429N04Rik; Ly110; Tscot; TSO-1C12"
                     /inference="alignment:Splign:2.1.0"
     CDS             136..1575
                     /gene="Slc46a2"
                     /gene_synonym="5430429N04Rik; Ly110; Tscot; TSO-1C12"
                     /note="putative thymic stromal co-transporter TSCOT;
                     thymic stromal cotransporter protein; thymic stromal
                     cotransporter homolog"
                     /codon_start=1
                     /product="solute carrier family 46 member 2"
                     /protein_id="NP_066395.3"
                     /db_xref="CCDS:CCDS18227.1"
                     /db_xref="GeneID:30936"
                     /db_xref="MGI:MGI:1353616"
                     /translation="MGPGGTCPWSSRLSGFRVRTWIEPVVASTQVAGSLYDAGLLLVV
                     KESFKSEAGGSSNYSANQSLVEYQEDQQQKAISNFNIIYNLVLGLTPLLSAYGLGWLS
                     DRYHRKISICTAMLGFLLSRIGLLLKVMLDWPVEVMYGAAALNGLCGSFSAYWSGVMA
                     LGSLGCSEGRRSVRLILIDLVLGLAGFSGSMASGHLFKQIVGHSAQGLLLTACSVGCA
                     AFALFYSLFVLKVPESKPNKVHPTVDTVSGMMGTYRTLDPDQQDKQNVPRNPRTPGKG
                     KSSQREVVALLFVGAIIYDLAAVGTVDVMALFVLKEPLHWNQVQLGYGMASGYIIFIT
                     SFLGVLVFSRCFRDTTMIIIGMLSFGSGALLLAFVKETYMFYIARAIMLFALIPITTI
                     RSAMSKLIKDSSYGKIFVILQLCLTLTGVVTSTIYNKIYQLTLDKFIGTCFVLSSFLS
                     FLAIVPIGVVAYKQVPRSQQGECAEKQRS"
     exon            1268..1351
                     /gene="Slc46a2"
                     /gene_synonym="5430429N04Rik; Ly110; Tscot; TSO-1C12"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1508
                     /gene="Slc46a2"
                     /gene_synonym="5430429N04Rik; Ly110; Tscot; TSO-1C12"
                     /inference="alignment:Splign:2.1.0"
     exon            1509..2060
                     /gene="Slc46a2"
                     /gene_synonym="5430429N04Rik; Ly110; Tscot; TSO-1C12"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2006..2011
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc46a2"
                     /gene_synonym="5430429N04Rik; Ly110; Tscot; TSO-1C12"
                     /note="hexamer: AAGAAA"
     polyA_site      2053
                     /gene="Slc46a2"
                     /gene_synonym="5430429N04Rik; Ly110; Tscot; TSO-1C12"
                     /note="major polyA site"
     polyA_site      2059
                     /gene="Slc46a2"
                     /gene_synonym="5430429N04Rik; Ly110; Tscot; TSO-1C12"
                     /note="major polyA site"
ORIGIN      
        1 acacaagccg ccccagctct gctcttgaag cacgcacctg aaacacactc tgggattgag
       61 ccctgaccct ccaaattcgg gagcatctcc tagactctgc agacagcaag ccctgttccc
      121 tgtcacgcag gtcacatggg tccagggggc acctgtccgt ggagcagccg gctctctggc
      181 ttccgggtga ggacttggat tgagcctgtg gtggcctcaa cccaggtggc cggctccctc
      241 tacgacgcag gactactcct ggtagtgaag gagtccttca agtctgaggc tggaggctcc
      301 tctaattaca gtgccaacca gtcgctggtg gagtatcagg aagaccaaca gcagaaggct
      361 atctccaatt tcaacatcat ttacaacctc gtgctgggcc tgacgcctct gctgtccgcc
      421 tatggactgg gctggctcag tgaccgctac caccgtaaga tctctatctg cacagcgatg
      481 ctgggcttcc tgctgtcccg catcggactc ttactcaaag tgatgctgga ctggccagtg
      541 gaggtgatgt acggagcagc agcgctcaat gggctatgcg ggagcttctc cgcttattgg
      601 tccggggtca tggcgctggg atccctgggc tgctccgaag gccgccgctc cgtgcgcctc
      661 atcctcatcg atttagtcct gggcttggct gggttctctg ggagcatggc ttcggggcat
      721 ctcttcaagc aaatcgttgg gcactctgcg caaggcctcc tgttaactgc ctgcagcgtt
      781 ggctgtgcgg cgtttgccct tttctacagc ctctttgtgc tgaaggtccc tgagtccaag
      841 cccaacaagg tgcaccccac tgtagatact gtgtctggca tgatgggcac ctatcgaacc
      901 ctggatccag atcaacaaga caaacagaat gtaccaagga accctcggac tcctgggaaa
      961 gggaagtctt cccaaaggga agttgttgct ctgctgtttg tgggtgccat catctatgac
     1021 ctggctgctg tgggcacagt agacgtcatg gccctttttg tgcttaagga gcctctccat
     1081 tggaaccaag tgcagctggg ctatgggatg gcttctgggt acataatctt cataaccagc
     1141 ttcttgggtg tcttggtttt ctcccgctgc ttccgggaca ccacgatgat cataatcggg
     1201 atgctttcct ttgggtccgg agcccttctc ttggcctttg tgaaggagac atacatgttc
     1261 tacattgccc gagccatcat gctgtttgcg cttattccga tcacaaccat caggtcagcc
     1321 atgtccaaac tcataaagga ctcttcctat ggaaagattt tcgtcatact acagctgtgc
     1381 ctaactctga ctggggtggt gacatccacc atatataaca agatctatca gcttacgctg
     1441 gacaagttca ttggcacctg ctttgttcta tcatcttttc tctccttctt ggccattgtc
     1501 ccaattggtg ttgtggccta caaacaagtc ccgaggtcac aacaaggaga gtgtgcagag
     1561 aaacagagga gctgaaaacg ccagccataa ccaggttctg cctgtgaaac ccaagctcca
     1621 gtcttccaat aacctgcttt ggcctgcagg tccctggtgg gagggaaaat cactttctgg
     1681 gtgttggaga agatttccta gttctcagat gcctctgcca ggctgaggtt gaggtctgtg
     1741 tgtaccagct gcttgcacag gtgttctgga tcctggaggg cttcgggctc cagccagcca
     1801 gccccctttt ctcattgctg tctaaaagac gccaatgggg cacatggaat cgtgctagtt
     1861 ctatggaaga ctgtgactaa agcctaaatt taaccaaccc aaggggaatg aacatgaaat
     1921 ggacattttg taaaaactga caactaaaaa cagttgtatg catgacaaca gtttttaaat
     1981 taatgtaaac tctttagaaa aaaaaaagaa aaaaaaaaga gttttcttgg ctgagcattc
     2041 ctcagctgga agtcaaggtg
//
