LOCUS       NM_001359227            3629 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus solute carrier family 44, member 2 (Slc44a2),
            transcript variant 3, mRNA.
ACCESSION   NM_001359227 XM_006510575
VERSION     NM_001359227.1
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3629)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3629)
  AUTHORS   Nair,T.S., Kakaraparthi,B.N., Yang,L., Lu,L., Thomas,T.B.,
            Morris,A.C., Kommareddi,P., Kanicki,A. and Carey,T.E.
  TITLE     Slc44a2 deletion alters tetraspanin and N-cadherin expression:
            Reduced adhesion and enhanced proliferation in cultured mesenchymal
            lung cells
  JOURNAL   Tissue Cell 73, 101599 (2021)
   PUBMED   34371293
  REMARK    GeneRIF: Slc44a2 deletion alters tetraspanin and N-cadherin
            expression: Reduced adhesion and enhanced proliferation in cultured
            mesenchymal lung cells.
REFERENCE   3  (bases 1 to 3629)
  AUTHORS   Bennett,J.A., Mastrangelo,M.A., Ture,S.K., Smith,C.O.,
            Loelius,S.G., Berg,R.A., Shi,X., Burke,R.M., Spinelli,S.L.,
            Cameron,S.J., Carey,T.E., Brookes,P.S., Gerszten,R.E.,
            Sabater-Lleal,M., de Vries,P.S., Huffman,J.E., Smith,N.L.,
            Morrell,C.N. and Lowenstein,C.J.
  TITLE     The choline transporter Slc44a2 controls platelet activation and
            thrombosis by regulating mitochondrial function
  JOURNAL   Nat Commun 11 (1), 3479 (2020)
   PUBMED   32661250
  REMARK    GeneRIF: The choline transporter Slc44a2 controls platelet
            activation and thrombosis by regulating mitochondrial function.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3629)
  AUTHORS   Tilburg,J., Coenen,D.M., Zirka,G., Dolleman,S., van
            Oeveren-Rietdijk,A.M., Karel,M.F.A., de Boer,H.C.,
            Cosemans,J.M.E.M., Versteeg,H.H., Morange,P.E., van Vlijmen,B.J.M.,
            Maracle,C.X. and Thomas,G.M.
  TITLE     SLC44A2 deficient mice have a reduced response in stenosis but not
            in hypercoagulability driven venous thrombosis
  JOURNAL   J Thromb Haemost 18 (7), 1714-1727 (2020)
   PUBMED   32297475
  REMARK    GeneRIF: SLC44A2 deficient mice have a reduced response in stenosis
            but not in hypercoagulability driven venous thrombosis.
REFERENCE   5  (bases 1 to 3629)
  AUTHORS   Tilburg,J., Michaud,S.A., Maracle,C.X., Versteeg,H.H.,
            Borchers,C.H., van Vlijmen,B.J.M. and Mohammed,Y.
  TITLE     Plasma Protein Signatures of a Murine Venous Thrombosis Model and
            Slc44a2 Knockout Mice Using Quantitative-Targeted Proteomics
  JOURNAL   Thromb Haemost 120 (3), 423-436 (2020)
   PUBMED   32135565
  REMARK    GeneRIF: Plasma Protein Signatures of a Murine Venous Thrombosis
            Model and Slc44a2 Knockout Mice Using Quantitative-Targeted
            Proteomics.
REFERENCE   6  (bases 1 to 3629)
  AUTHORS   Tilburg,J., Adili,R., Nair,T.S., Hawley,M.E., Tuk,D.C., Jackson,M.,
            Spronk,H.M., Versteeg,H.H., Carey,T.E., van Vlijmen,B.J.M.,
            Maracle,C.X. and Holinstat,M.
  TITLE     Characterization of hemostasis in mice lacking the novel thrombosis
            susceptibility gene Slc44a2
  JOURNAL   Thromb Res 171, 155-159 (2018)
   PUBMED   30312801
  REMARK    GeneRIF: Mice lacking SLC44A2 are normal for several hemostasis
            parameters. Lower levels of circulating plasma VWF are measured in
            SLC44A2 deficient mice.
REFERENCE   7  (bases 1 to 3629)
  AUTHORS   Kommareddi,P., Nair,T., Kakaraparthi,B.N., Galano,M.M., Miller,D.,
            Laczkovich,I., Thomas,T., Lu,L., Rule,K., Kabara,L., Kanicki,A.,
            Hughes,E.D., Jones,J.M., Hoenerhoff,M., Fisher,S.G.,
            Altschuler,R.A., Dolan,D., Kohrman,D.C., Saunders,T.L. and
            Carey,T.E.
  TITLE     Hair Cell Loss, Spiral Ganglion Degeneration, and Progressive
            Sensorineural Hearing Loss in Mice with Targeted Deletion of
            Slc44a2/Ctl2
  JOURNAL   J Assoc Res Otolaryngol 16 (6), 695-712 (2015)
   PUBMED   26463873
REFERENCE   8  (bases 1 to 3629)
  AUTHORS   Bougie,D.W., Peterson,J.A., Kanack,A.J., Curtis,B.R. and Aster,R.H.
  TITLE     Transfusion-related acute lung injury-associated HNA-3a antibodies
            recognize complex determinants on choline transporter-like protein
            2
  JOURNAL   Transfusion 54 (12), 3208-3215 (2014)
   PUBMED   24846273
  REMARK    GeneRIF: The findings show that, for binding to CTL2, Type 2 HNA-3a
            antibodies require nonpolymorphic amino acid residues in the third,
            and possibly the second, EC loops of CTL2
REFERENCE   9  (bases 1 to 3629)
  AUTHORS   Lo,J.C., Jamsai,D., O'Connor,A.E., Borg,C., Clark,B.J.,
            Whisstock,J.C., Field,M.C., Adams,V., Ishikawa,T., Aitken,R.J.,
            Whittle,B., Goodnow,C.C., Ormandy,C.J. and O'Bryan,M.K.
  TITLE     RAB-like 2 has an essential role in male fertility, sperm
            intra-flagellar transport, and tail assembly
  JOURNAL   PLoS Genet 8 (10), e1002969 (2012)
   PUBMED   23055941
REFERENCE   10 (bases 1 to 3629)
  AUTHORS   Beyer,L.A., Galano,M.M., Nair,T.S., Kommareddi,P.K., Sha,S.H.,
            Raphael,Y. and Carey,T.E.
  TITLE     Age-related changes in expression of CTL2/SLC44A2 and its isoforms
            in the mouse inner ear
  JOURNAL   Hear Res 282 (1-2), 63-68 (2011)
   PUBMED   21986210
  REMARK    GeneRIF: Report age related changes between the two isoforms of
            CTL2/SLC44A2 in the inner ear.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC122525.4.
            
            On Dec 29, 2017 this sequence version replaced XM_006510575.2.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.61667.1,
                                           SRR9219380.93416.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-133               AC122525.4         90869-91001         c
            134-182             AC122525.4         73231-73279         c
            183-256             AC122525.4         69868-69941         c
            257-341             AC122525.4         69697-69781         c
            342-426             AC122525.4         69453-69537         c
            427-537             AC122525.4         69215-69325         c
            538-598             AC122525.4         69062-69122         c
            599-722             AC122525.4         68854-68977         c
            723-806             AC122525.4         68673-68756         c
            807-919             AC122525.4         68473-68585         c
            920-1051            AC122525.4         66757-66888         c
            1052-1151           AC122525.4         66328-66427         c
            1152-1244           AC122525.4         66149-66241         c
            1245-1329           AC122525.4         65727-65811         c
            1330-1592           AC122525.4         64769-65031         c
            1593-1687           AC122525.4         64591-64685         c
            1688-1791           AC122525.4         63734-63837         c
            1792-1865           AC122525.4         63572-63645         c
            1866-1936           AC122525.4         63322-63392         c
            1937-2025           AC122525.4         63094-63182         c
            2026-2110           AC122525.4         59169-59253         c
            2111-2282           AC122525.4         58548-58719         c
            2283-3629           AC122525.4         56692-58038         c
FEATURES             Location/Qualifiers
     source          1..3629
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 7.77 cM"
     gene            1..3629
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /note="solute carrier family 44, member 2"
                     /db_xref="GeneID:68682"
                     /db_xref="MGI:MGI:1915932"
     exon            1..133
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     CDS             103..2235
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /note="isoform 3 is encoded by transcript variant 3;
                     choline transporter-like protein 2"
                     /codon_start=1
                     /product="choline transporter-like protein 2 isoform 3"
                     /protein_id="NP_001346156.1"
                     /db_xref="GeneID:68682"
                     /db_xref="MGI:MGI:1915932"
                     /translation="MEDDRKDAVYGTPQKYDPTFKGPIYNRGCTDVICCVLLFLAIVG
                     YVAVGIIAWTHGDPRKVIYPTDSRGEFCGQKGTKNADKPFLFYFNIVKCANPLVLLEF
                     HCPTPQICVKQCPDRYLTLLSARNTRDFDYYKQFCVPGFQNNKGVTEILRDGECPAVI
                     TPSKPLAQRCFPAIHASKGVLMVGNETTYEDGHGARKNITDLVEGAKKANKILEARQL
                     AMQIFEDYTVSWYWIIIGLVIAMVLSLLFIVLLRFLAGIMVWVMIVMVILVLGYGIFH
                     CYMEYSRLRGEAGSDVSLVDLGFQTDLRVYLHLRQTWMAFMIILSILEVVIILLLIFL
                     RKRILIAIALIKEASRAVGHVMCSLLYPLVTFFLLCLCIAYWASTSVFLSTSNTAVYK
                     VVDDTACPLLRKTCNPETFPLRNESLQCPTARCQFAFYGGESTYHRALLGLQIFNAFM
                     FFWLANFVLALGQVTLAGAFASYYWAMRKPDDMPAFPLFSAFGRALRYHTGSLAFGSL
                     ILAIVQIIRVMLEYLDQRLKAAQNKFAKFLMVCLKCCFWCLEKFIKFLNRNAYIMIAI
                     YGTNFCTSARNAFFLLMRNIIRVAVLDKVTDFLFLLGKLLIVGSVGILAFFFFTHRIR
                     IVQDTAPPLNYYWVPILTVIIGSYLIAHGFFSVYGMCVDTLFLCFCEDLERNDGSQER
                     PYFMSPELRGILLKGNPQEGKQEEVEEE"
     exon            134..182
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            183..256
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            257..341
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            342..426
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            427..537
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            538..598
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            599..722
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            723..806
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            807..919
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            920..1051
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1052..1151
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1152..1244
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1245..1329
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1330..1592
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1593..1687
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1688..1791
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1865
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1866..1936
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            1937..2025
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2026..2110
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2111..2282
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     exon            2283..3629
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3606..3611
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /note="hexamer: AATAAA"
     polyA_site      3629
                     /gene="Slc44a2"
                     /gene_synonym="1110028E10Rik; CTL2"
                     /note="major polyA site"
ORIGIN      
        1 ggtgtcaggc ggggcagggc ggggccagca gcgcgcggtg tctcccgccc gcttgggttg
       61 gggtcgcgct ggcttcggac tcagctctcc tcggttgagg ccatggagga cgatcggaag
      121 gacgcagtct atgggacgcc tcagaaatac gaccccacct tcaaaggacc catttacaac
      181 aggggatgca cagatgtcat ttgctgtgtg ttgctcttcc tggccatcgt aggctatgtg
      241 gctgtgggca tcatagcctg gacccatgga gaccctcgga aggtgatcta ccccactgat
      301 agccggggcg aattctgtgg gcaaaagggg acaaaaaatg cggacaaacc gttcttgttt
      361 tacttcaaca tcgtgaagtg tgccaacccc ctggtcctgc tggaatttca ctgtcccacc
      421 cctcagatct gcgtgaagca gtgtccagac cgctatctca cccttctgag tgcgcgcaac
      481 actcgcgact ttgactatta caagcagttc tgtgtgcctg gcttccagaa caataaaggg
      541 gtgactgaga tactccgaga tggggagtgt ccagcggtta tcacccccag caaacctttg
      601 gcccagcggt gctttcccgc catccatgcc agcaaaggag tcctcatggt gggcaatgag
      661 acgacctatg aggacggaca tggcgccaga aagaacatta cagatctagt ggagggtgcc
      721 aaaaaggcca acaagattct agaggcccgg caactcgcca tgcagatatt tgaagattac
      781 actgtatcct ggtactggat tatcataggt ctggtcattg ccatggtgct gagtctcctg
      841 ttcatcgtcc tgctgcgatt cctggctggc attatggtct gggtgatgat tgtcatggtg
      901 atcctggtgc ttggctatgg catcttccac tgctacatgg agtactcgag actccgtgga
      961 gaggcaggct ccgacgtctc cctggtggac cttggcttcc agacggacct ccgagtgtac
     1021 ctgcacttga ggcagacctg gatggctttt atgatcattt taagcatcct ggaagtggtc
     1081 atcatcctgt tacttatctt tctgcgcaaa aggatattga tcgccatcgc cctcatcaaa
     1141 gaagccagca gggctgtggg acatgtcatg tgctccctgc tctacccact ggtgaccttc
     1201 ttcctcttgt gtctctgcat tgcctactgg gccagcactt ctgtattcct gtccacctcc
     1261 aacaccgccg tgtacaaggt tgttgatgac acagcctgcc cacttctgag gaaaacctgc
     1321 aacccagaga ccttcccctt gcggaatgag agcctccagt gtcccactgc ccgctgccag
     1381 tttgccttct atggtggcga atcgacctac caccgtgccc tgctgggcct gcagatcttc
     1441 aatgcattca tgttcttctg gctagccaac ttcgtgctgg ccctaggcca ggtaaccctg
     1501 gccggagcct ttgcctccta ttactgggct atgcggaagc ctgatgatat gcctgccttc
     1561 cccctcttct ctgcttttgg ccgagcactc aggtaccaca cgggctcctt agcttttggc
     1621 tcccttatct tagccatcgt gcagatcatc cgagtgatgc tggaatacct ggaccagcgc
     1681 ctaaaagctg cacagaacaa gtttgccaag ttcctcatgg tctgtctcaa gtgctgcttc
     1741 tggtgcctag agaagtttat caaattcctg aataggaatg cctacatcat gattgctatc
     1801 tatggcacca acttctgtac ctcagccagg aatgccttct tcctgcttat gagaaatatc
     1861 atcagggtgg ccgtcctgga caaagttaca gacttcctct tcctgttggg caaacttctg
     1921 attgtgggta gtgtgggcat cctggccttc ttcttcttca cccatcggat cagaattgtg
     1981 caagatacag ccccacctct caattattac tgggttccga tcctgacagt gatcattggc
     2041 tcctacttga tcgcgcacgg cttcttcagc gtctatggca tgtgcgtgga cacgctgttc
     2101 ctctgtttct gtgaggacct tgagaggaat gacggctctc aggagcgacc ctacttcatg
     2161 tcgcctgagc tgagaggcat cctattgaag gggaatccgc aggagggcaa gcaggaggaa
     2221 gtggaggagg agtagacagt gagggagccg ggcaagctgg ccagggggcc tctggagacc
     2281 ggtggaggat ctggagagaa atgacggttc agccgagagg ccttacttca tgtcttccac
     2341 tctcaagaaa ctcttgaata aaaccaacaa gaaagtagca gaatcctaaa gacacctgcc
     2401 tcccagccct ctcaggacgc cgggataggt gcttgctgtg cagctgggtc agagtcccca
     2461 gctcatcaga cttgcctaac acatcactgc ctctctgcac cctccccgag ttcccatttc
     2521 caggaaccta gaggactggg gcatctcttt cctatgccaa agggcacttg ggagttttca
     2581 gtgcagccct ggaagatggg acagaatgga tctctccagc tcttactggc tcagatggtg
     2641 ccaatatggt gaagtaactt gaagtcgagg atgggagatg gccctaggat gtctgcagca
     2701 agcttctttg tatttcagta acccttctgg ccaagaggga aagactggat gagagtgggc
     2761 aagtcagctc agcgaacact agcgccaagc tccccagcgg ggacagaatt ggatctccat
     2821 agctgatgta cctaagggcc agcaaataca cgtctcactc ttagtccttt gtagcctagg
     2881 cagacagtaa gggatctacc tgcttctctt ccatcaggct ccagaactgg atggagccac
     2941 caagagtgcc ttcatatgta accccacctc cagcgtgggc tggctatggt cacaggaaga
     3001 agtgtgcacc cagaactgga ggagtcttct ggataaacag acagcatctg cgaggagggc
     3061 cctgtgtgag gaacgattgg tgttgctgct ggtggttggg agtagttgtg gaaagcaggc
     3121 ctctgtactt agaccctaca gagttgcttt gactgatgac ttctaaatgg aaaacttgag
     3181 gttggaaaat gaatgaaaat agggcctgtc tccccagccc tttttctgcc agtaatgcac
     3241 actgctctct ttgtaagaga tgagaaactt aagttcaccc aagggatggt ttcagggact
     3301 gaggtgtggg tgtgttctat attccaaatt aactaggcct atctggtttc tttcacttga
     3361 aactagagaa tctgtttgcc aaacttcttg atcttgggag ccagtagact ggttcttcac
     3421 tttacctacc gtatgctaag gcccgcccct cacccctact ttggccatcc gtaagatagc
     3481 aggatcttgg agacaccgct cccagcctga ggtcccaagg tttcctgtgc ctgccttgtg
     3541 tgccatccca acccaccctc ttgtatgccc agccctgtgc ttatgtaatt gcattccggc
     3601 cagttaataa agtgcctctt gtacagaga
//
