LOCUS       NM_172289               5567 bp    mRNA    linear   ROD 02-MAY-2024
DEFINITION  Mus musculus solute carrier family 36 (proton/amino acid
            symporter), member 4 (Slc36a4), transcript variant 1, mRNA.
ACCESSION   NM_172289
VERSION     NM_172289.5
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5567)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5567)
  AUTHORS   Shang,P., Valapala,M., Grebe,R., Hose,S., Ghosh,S., Bhutto,I.A.,
            Handa,J.T., Lutty,G.A., Lu,L., Wan,J., Qian,J., Sergeev,Y.,
            Puertollano,R., Zigler,J.S. Jr., Xu,G.T. and Sinha,D.
  TITLE     The amino acid transporter SLC36A4 regulates the amino acid pool in
            retinal pigmented epithelial cells and mediates the mechanistic
            target of rapamycin, complex 1 signaling
  JOURNAL   Aging Cell 16 (2), 349-359 (2017)
   PUBMED   28083894
  REMARK    GeneRIF: the amino acid transporter SLC36A4/proton-dependent amino
            acid transporter (PAT4) regulates the amino acid pool in the
            lysosomes of retinal pigment epithelium.
REFERENCE   3  (bases 1 to 5567)
  AUTHORS   Sharma,N., Kubaczka,C., Kaiser,S., Nettersheim,D., Mughal,S.S.,
            Riesenberg,S., Holzel,M., Winterhager,E. and Schorle,H.
  TITLE     Tpbpa-Cre-mediated deletion of TFAP2C leads to deregulation of
            Cdkn1a, Akt1 and the ERK pathway, causing placental growth arrest
  JOURNAL   Development 143 (5), 787-798 (2016)
   PUBMED   26811378
REFERENCE   4  (bases 1 to 5567)
  AUTHORS   Roshanbin,S., Hellsten,S.V., Tafreshiha,A., Zhu,Y., Raine,A. and
            Fredriksson,R.
  TITLE     PAT4 is abundantly expressed in excitatory and inhibitory neurons
            as well as epithelial cells
  JOURNAL   Brain Res 1557, 12-25 (2014)
   PUBMED   24530433
  REMARK    GeneRIF: Detailed analysis of Slc36a4 mRNA distribution and protein
            mapping in mouse brain, showing an abundant expression in piriform
            cortex, hippocampus and hypothalamus in inhibitory and excitatory
            neurons and epithelial cells lining the ventricles in the brain
REFERENCE   5  (bases 1 to 5567)
  AUTHORS   Matsui,T. and Fukuda,M.
  TITLE     Rab12 regulates mTORC1 activity and autophagy through controlling
            the degradation of amino-acid transporter PAT4
  JOURNAL   EMBO Rep 14 (5), 450-457 (2013)
   PUBMED   23478338
  REMARK    GeneRIF: A new mechanism of regulation of mTORC1 signalling and
            autophagy, that is, quality control of PAT4 by Rab12.
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC154307.2 and AK032074.1.
            
            On Nov 4, 2017 this sequence version replaced NM_172289.4.
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR9219379.41894.1,
                                           SRR9219380.11450.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN00849374, SAMN00849375
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-31                AC154307.2         131241-131271       c
            32-1968             AK032074.1         2-1938
            1969-5567           AC154307.2         98621-102219        c
FEATURES             Location/Qualifiers
     source          1..5567
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="9"
                     /map="9 5.12 cM"
     gene            1..5567
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /note="solute carrier family 36 (proton/amino acid
                     symporter), member 4"
                     /db_xref="GeneID:234967"
                     /db_xref="MGI:MGI:2442595"
     exon            1..100
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1557
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /note="isoform 1 is encoded by transcript variant 1;
                     proton-coupled amino acid transporter 4; proton/amino acid
                     transporter 4; solute carrier family 36 member 4; neutral
                     amino acid uniporter 4"
                     /codon_start=1
                     /product="neutral amino acid uniporter 4 isoform 1"
                     /protein_id="NP_758493.2"
                     /db_xref="CCDS:CCDS22840.1"
                     /db_xref="GeneID:234967"
                     /db_xref="MGI:MGI:2442595"
                     /translation="MEAPAPAETAGCEELDMDVMRPLINEQNFDGSSDEEQEQTLVPI
                     QKHYQLDGQHGISFLQTLVHLLKGNIGTGLLGLPLAIKNAGIVLGPISLVFIGIISVH
                     CMHILVRCSHFLCQRFKKSTLGYSDTVSFAMEASPWSCLQRQAAWGRSVVDFFLVITQ
                     LGFCSVYIVFLAENVKQVHEGFLGSTPIVSNGSDLSHACERRSVDLRVYMLCFLPLII
                     LLVFIRELKNLFVLSFLANISMAASLVIIYQYVVRNMPDPHNLPIVAGWKKYPLFFGT
                     AVFAFEGIGVVLPLENQMRESKRFPQALNIGMAIVTVLYISLATLGYMCFRDEIKGSI
                     TLNLPQDMWLYQSVKILYSFGIFVTYSIQFYVPAEIIIPGVTARLHAKWKRICEFGIR
                     SLLVSITCAGAILIPRLDIVISFVGAVSSSTLALILPPLVEILTFSKDHYNIWMILKN
                     ISIAFTGVVGFLLGTYVTVEEIIYPTTAVVAGTSQSPFLNVNSTCITSGL"
     exon            101..224
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            225..315
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            316..404
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            405..500
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            501..585
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            586..813
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            814..912
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            913..1082
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1083..1252
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     exon            1253..5567
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5536..5541
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /note="hexamer: AATAAA"
     polyA_site      5563
                     /gene="Slc36a4"
                     /gene_synonym="6330573I15Rik; PAT4"
                     /note="major polyA site"
ORIGIN      
        1 gctcggcggt gcggccggca gagcgggcgg cggcgacctg gagacccccg agggatggaa
       61 gcgccggcgc cggcggagac ggcgggatgc gaggagctcg atatggacgt gatgaggccc
      121 ttaataaacg agcagaattt cgatgggtcg tccgacgagg agcaggaaca gacgcttgtg
      181 cccatacaga agcactacca gctcgatggg cagcacggga tttcgttcct gcagaccttg
      241 gtgcatcttc tcaaggggaa catcgggact ggccttttag ggctcccctt ggctataaag
      301 aacgcgggca tcgtgcttgg accaatcagc cttgtgttta taggaattat ttccgtccac
      361 tgtatgcaca tattggtacg ttgcagtcac tttctatgtc agaggtttaa gaagtcaaca
      421 ttggggtaca gtgacaccgt gagttttgcc atggaggcta gtccgtggag ttgccttcag
      481 cgacaggcag catgggggcg gagcgtggtc gacttctttc tggtgataac acagctggga
      541 ttctgcagcg tctacattgt cttcttagct gagaatgtga aacaggttca tgaaggattc
      601 ctggggagca cgccgattgt ttcaaatggc tcggacctgt ctcatgcctg tgagagaaga
      661 agtgtggacc tcagggtcta tatgctctgc tttctcccac ttataattct cttggtcttc
      721 attcgcgagc tgaagaatct ctttgtactt tcattccttg ccaacatttc catggctgcc
      781 agtcttgtga taatttacca gtatgttgtt aggaacatgc cagatcccca caaccttcca
      841 atagtggccg gctggaagaa atacccactg ttttttggca ctgctgtgtt tgcttttgaa
      901 ggcataggag tggtgctacc actggaaaac caaatgagag agtccaaacg cttccctcag
      961 gcattgaaca tcggcatggc catcgtcacc gtgctgtaca tcagcctagc cactttaggc
     1021 tacatgtgtt tccgtgacga gatcaaaggc agcattacac tgaatcttcc ccaggacatg
     1081 tggttgtatc agtcagtgaa aattctgtat tcctttggca tttttgtgac ctattcaatt
     1141 cagttctatg tcccagcaga gatcattatc cctggagtca ctgctagact tcatgccaaa
     1201 tggaagcgca tttgtgaatt tgggatacgg tccctcttgg ttagtatcac ctgtgctggg
     1261 gcgattctca ttcctcgcct agacattgtg atctccttcg tgggggctgt gagcagcagt
     1321 acactagccc tgatcctgcc cccacttgtg gaaatcctta cattttctaa ggaccactac
     1381 aacatatgga tgatcctgaa aaacatttcc atagcgttca ctggagtcgt gggcttcttg
     1441 ttgggcacct atgtcactgt tgaagaaatt atttatccga ctacggcagt tgttgctggc
     1501 acctcccaga gtccctttct gaatgtgaac tctacatgca taacaagtgg tttgtaatca
     1561 tgggagaaga gcggtgaacc ctctcctgtc accccaattc taacagcagt caagaacgac
     1621 tacagtgctt ccccatacac tgaacagtgc caaactccta gtttttgttg gcagagtatt
     1681 gcaatttgac tgtgaactgg acatcttttg cagctatgat aaactatgga agattattgc
     1741 ttttatctaa tgacaatgaa aaattgaaaa aatttaaaat tcaaaatgta aacaataagc
     1801 acagcccatt attgtgtttc tttaagaaac actttgagta agagttgtgt ttcttaccac
     1861 tattgagaag ataaaggatt tcaacatggt ataaattaaa taggcacatg gtttaaaaac
     1921 ctctttgaag caccccacaa tagcaaagta cacctcctgt gtatgtgcac agagatgcgt
     1981 tgattgtgtt ttattccatg ggtgatgctg ctgacaagcc ctgaaggtac agtagactcc
     2041 agctgaccac cgtgcaagca ttagatagtt cccagagcca gaagcgctct attgttctgc
     2101 tctacagagc atagaggagc actgggaagc actgacaagg ttctgacttc ctctttcagc
     2161 cgtaggtgaa agggccagtg aggacctgga acattgctgc cctagctgtg tcttgttagg
     2221 gtttctgttg ctgtgagaaa acaccacaac caaaagtaat tatggaggag agggcttctg
     2281 tttacacttc cacatcacaa tccatcatca agggaagtca ggacaggaac tcaaggcagg
     2341 aacctggagg caggagctga tgcagaggcc aaggaggagt gctgcttact ggcttgcttc
     2401 tttctcatgg cttgctcagc ctgctttctt acagcacaca gggcaaccag cccaggggtg
     2461 gtgccgccca cagtgggcat agccctctca gagcaatcat caatctggag gatgccccac
     2521 agcctctcct ctcttccaaa atgactccag attgtgttaa gttgacataa atctagccag
     2581 catggttacc attgtaggaa atggagttcc agaagtgttg gtgacttaag cttgagtcaa
     2641 gagaacctga tacttgaccc aattcagaga tggaagaaaa atgatttttc atgtcatagt
     2701 cagtaatgat aaaacacatt cagcttaatt ttagtgtaaa gcttaattat agtttcatcc
     2761 tttgggagtg tgtgtcttat gtctgaatag tatatgtgac atgataaaaa gccagaaaag
     2821 aaatccaaga aacctgcctg gtggctccat agatcccaaa cagggaaaga agctcacagt
     2881 cttttataca gcaggagact ggcttgctgt agggtgtgct gtagggcaga ggtcacagga
     2941 ggtgaggccc cttaaacagt aggagactgc gggctggctg tgtagtgttc tagtcacacg
     3001 cttccttgtc tcctaggttt gcaggcatga taaatacctg gcaaaataca gaacttttgg
     3061 atttcctagc catgtagcat tccttactgc agtcttccat taatctgaat gggcagagta
     3121 aaggaactgc caaatgaacg cttaggaaga tgcaaaacaa tagagaactg tctagtaaat
     3181 actggagcac ttaggcattg tccagagaga tgtggccttc accctgtctt caaacacact
     3241 agccaaagca accaggctca tctgatgctg aagaggagaa ctggggagtt tgaaggactg
     3301 agcggggcca tctcctggac atctttgccc attggctcta tactgtgtta gctggaagat
     3361 ggtaccaagc tttctgtgga gttctgtatt ttctttatgt aaatgttaac ttattcaggt
     3421 gggctttgct tatgtcgtta ctttgccttc aggtgtgggc ctataaacat atcagaagct
     3481 ttattttgct atgcattcat aagaaccaaa actttataac atttatgtta gatatttcta
     3541 gtaattgttt atattatttt atgttaaatt gttacttcaa gtcttcagtc ttaggcagtg
     3601 aatgaggatg tgaagaaagg aatttcattg gaatttgact gtgagaaatg tacagtggca
     3661 cagggtgagt tacaaactcc ccgcccagga agcagcatgt ggctctttgt cagctgtgtt
     3721 cacggtgtgt tctactcggc atgctcagtt tatttttgtg ttttcacctg ttccttgtgg
     3781 aattcaagtg cagtgggttt ttaactggtg ggtcactgca gggcttttta agttccacac
     3841 acagagtgtg agcatcttat cagcccggat ttcttatgga ggaaggctgc tgaatcaaca
     3901 gctcatatga aagtccccag gttatcaaca caagaggagt cacaatacag aaaggaagat
     3961 atgctgggag aagaggagat agttttaatt tgtagtgtga gccactgttt atatgcaaag
     4021 tataaaagaa acacacactt gtttaagggt gtctgaggac gtatttacca tttgatcatt
     4081 tttctctgat atttggcttt acagaattct atcttctaaa gatttttgga gaagatatgt
     4141 tgactctagc agctcttcct tttgtttggg gacttggagg gaggggaagc aggcacacat
     4201 ggcgaactcg tgtttgctgg atcacatggt gactgcagtg accccagcag ttacttcctg
     4261 tcatggtggg aactgtccct ggaagctagt cagttgcata ggtgctattt tctctgtgaa
     4321 gcattgagaa gaaagctgtt gcttactccc aatggctctc tgttccctga tgctccgagg
     4381 aatgcattgg tgtctgagtc cactcaaaac actgttccca caggactttt taacacaggc
     4441 tctttccctc catgtgtccc cctttctcca cagttgaaga agtcggagct gggaagtttt
     4501 caggttagat gtgccagtta atgtaaagaa agctatgttt gtggtgctcc agtactttcc
     4561 tgttctgttc tttctgacct gtcaccaaag ctggctctga gtcttgttag atggtgtact
     4621 taaagtgttc ataagccatt tatggccgtg cgcttgtgaa tttcctatca gggtttgatc
     4681 ttatttggta tatgtgcatt tgtggtccta gagaatgcca gcttaagtgc acatcgatac
     4741 ccttgctaac taggttttac atagctcacc atttcactgt ggtgcccaca gatgcagcac
     4801 atctgttact ggcagcatac attaggcctt gtgtgcgact cctttttttt tttcagtcaa
     4861 gatgggaaag atacagaaca gagccagtgg aaataataaa tgcagttgtt tatataacat
     4921 gagtggttca gcagatgtta actgttgagt gataaaactg ttataaagca tcaataatgt
     4981 aaaattataa aatgttggaa attgtgttta tttgactagc ttggctcagt tatcttccct
     5041 tagttaagaa aatgtaaggg atttatctag tgcagataac aggattcttc cggtcattaa
     5101 atggctgcat gttactgcca tacatgactt tctgcatctc gttggttctg acctaggcta
     5161 taaaagaaat atgcttaaga aatggctagg tctagaattt atcgcctatc gagtgaaatg
     5221 acaaatcacc caattatttg gatttcctgt tagaattggc tcgttcctgt gtgcatccat
     5281 gcatgttaca ccacagggac tcacatctga atccgtttca actactctac tccagatagg
     5341 accagatggt gatcacatag gaaaatccat attcatgaca tgaacagttc aggctataga
     5401 tttttgtttt tcaaaagtga ttttgtaagc tttattgaaa gtaaagttgt attggagttg
     5461 tatcatttct tatatttatt tttctttgta aacgagtttg aaaatgccta aaagtactgt
     5521 gacttctgaa ttgtcaataa agtataatta ttttgcttgc taataaa
//
