LOCUS       NM_011774               5497 bp    mRNA    linear   ROD 30-APR-2024
DEFINITION  Mus musculus solute carrier family 30 (zinc transporter), member 4
            (Slc30a4), transcript variant 1, mRNA.
ACCESSION   NM_011774
VERSION     NM_011774.3
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 5497)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 5497)
  AUTHORS   Wang,P., Pan,R., Weaver,J., Jia,M., Yang,X., Yang,T., Liang,J. and
            Liu,K.J.
  TITLE     MicroRNA-30a regulates acute cerebral ischemia-induced blood-brain
            barrier damage through ZnT4/zinc pathway
  JOURNAL   J Cereb Blood Flow Metab 41 (3), 641-655 (2021)
   PUBMED   32501158
  REMARK    GeneRIF: MicroRNA-30a regulates acute cerebral ischemia-induced
            blood-brain barrier damage through ZnT4/zinc pathway.
REFERENCE   3  (bases 1 to 5497)
  AUTHORS   McCormick,N.H., Lee,S., Hennigar,S.R. and Kelleher,S.L.
  TITLE     ZnT4 (SLC30A4)-null ('lethal milk') mice have defects in mammary
            gland secretion and hallmarks of precocious involution during
            lactation
  JOURNAL   Am J Physiol Regul Integr Comp Physiol 310 (1), R33-R40 (2016)
   PUBMED   26538236
  REMARK    GeneRIF: Loss of ZnT4 has profound consequences on mammary
            epithelial cell secretion and may promote tissue remodeling in the
            mammary gland during early lactation.
REFERENCE   4  (bases 1 to 5497)
  AUTHORS   McCormick,N.H. and Kelleher,S.L.
  TITLE     ZnT4 provides zinc to zinc-dependent proteins in the trans-Golgi
            network critical for cell function and Zn export in mammary
            epithelial cells
  JOURNAL   Am J Physiol Cell Physiol 303 (3), C291-C297 (2012)
   PUBMED   22621784
  REMARK    GeneRIF: It was shown that that ZnT4 transports Zn into the
            trans-Golgi network, which is critical for key secretory functions
            of the mammary cell.
REFERENCE   5  (bases 1 to 5497)
  AUTHORS   Wang,Z.Y., Stoltenberg,M., Jo,S.M., Huang,L., Larsen,A.,
            Dahlstrom,A. and Danscher,G.
  TITLE     Dynamic zinc pools in mouse choroid plexus
  JOURNAL   Neuroreport 15 (11), 1801-1804 (2004)
   PUBMED   15257151
  REMARK    GeneRIF: Expression of zinc transporter ZnT4 in mouse choroid
            epithelial cells suggests that the choroid plexus plays an
            important role in regulation of zinc homeostasis in the brain.
REFERENCE   6  (bases 1 to 5497)
  AUTHORS   Lee,D.Y., Shay,N.F. and Cousins,R.J.
  TITLE     Altered zinc metabolism occurs in murine lethal milk syndrome
  JOURNAL   J Nutr 122 (11), 2233-2238 (1992)
   PUBMED   1432264
REFERENCE   7  (bases 1 to 5497)
  AUTHORS   Ackland,M.L. and Mercer,J.F.
  TITLE     The murine mutation, lethal milk, results in production of
            zinc-deficient milk
  JOURNAL   J Nutr 122 (6), 1214-1218 (1992)
   PUBMED   1588438
REFERENCE   8  (bases 1 to 5497)
  AUTHORS   Swank,R.T., Reddington,M., Howlett,O. and Novak,E.K.
  TITLE     Platelet storage pool deficiency associated with inherited
            abnormalities of the inner ear in the mouse pigment mutants muted
            and mocha
  JOURNAL   Blood 78 (8), 2036-2044 (1991)
   PUBMED   1912584
REFERENCE   9  (bases 1 to 5497)
  AUTHORS   Erway,L.C. and Grider,A. Jr.
  TITLE     Zinc metabolism in lethal-milk mice. Otolith, lactation, and aging
            effects
  JOURNAL   J Hered 75 (6), 480-484 (1984)
   PUBMED   6512240
REFERENCE   10 (bases 1 to 5497)
  AUTHORS   Piletz,J.E. and Ganschow,R.E.
  TITLE     Zinc deficiency in murine milk underlies expression of the lethal
            milk (lm) mutation
  JOURNAL   Science 199 (4325), 181-183 (1978)
   PUBMED   619449
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL772253.12, AK134245.1, CV556160.1, CO432407.1 and AW125676.1.
            
            On Mar 21, 2014 this sequence version replaced NM_011774.2.
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK134245.1, AF003747.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164139 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-51                AL772253.12        138309-138359       c
            52-860              AK134245.1         30-838
            861-1279            CV556160.1         25-443
            1280-2606           AK134245.1         1258-2584
            2607-2667           CO432407.1         533-593
            2668-5440           AK134245.1         2646-5418
            5441-5497           AW125676.1         1-57                c
FEATURES             Location/Qualifiers
     source          1..5497
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.65 cM"
     gene            1..5497
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /note="solute carrier family 30 (zinc transporter), member
                     4"
                     /db_xref="GeneID:22785"
                     /db_xref="MGI:MGI:1345282"
     exon            1..634
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /inference="alignment:Splign:2.1.0"
     CDS             244..1536
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /note="isoform a is encoded by transcript variant 1;
                     lethal milk protein; solute carrier family 30 member 4;
                     zinc transporter 4; solute carrier family 30 (zinc
                     transporter), member 4; zinc transporter 4; probable
                     proton-coupled zinc antiporter SLC30A4"
                     /codon_start=1
                     /product="probable proton-coupled zinc antiporter SLC30A4
                     isoform a"
                     /protein_id="NP_035904.2"
                     /db_xref="CCDS:CCDS16667.1"
                     /db_xref="GeneID:22785"
                     /db_xref="MGI:MGI:1345282"
                     /translation="MAGPGAWKRLKSLLRKDDTPLFLNDTSAFDFSDEVSDEGLSRFN
                     KLRVVVADDDSEAPERPVNGAHPALQADDDSLLDQDLPLTNSQLSLKMDPCDNCSKRR
                     ELLKQRKVKTRLTIAAVLYLLFMIGELVGGYMANSLAIMTDALHMLTDLSAIILTLLA
                     LWLSSKSPTRRFTFGFHRLEVLSAMISVMLVYVLMGFLLYEAVQRTIHMNYEINGDVM
                     LITAAVGVAVNVIMGFLLNQSGHHHSHAHSHSLPSNSPSMVSSGHNHGQDSLAVRAAF
                     VHALGDLVQSVGVLIAAYIIRFKPEYKIADPICTYIFSLLVAFTTFRIIWDTVVIILE
                     GVPSHLNVDYIKESLMKIEDVYSVEDLNIWSLTSGKSTAIVHMQLIPGSSSKWEEVQS
                     KAKHLLLNTFGMYKCTIQLQSYRQEVIRTCANCHSSST"
     exon            635..781
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /inference="alignment:Splign:2.1.0"
     exon            782..935
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /inference="alignment:Splign:2.1.0"
     exon            936..1140
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1246
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1247..1381
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /inference="alignment:Splign:2.1.0"
     exon            1382..5482
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5425..5430
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /note="hexamer: AATAAA"
     regulatory      5453..5458
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /note="hexamer: ATTAAA"
     polyA_site      5467
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
                     /note="major polyA site"
     polyA_site      5482
                     /gene="Slc30a4"
                     /gene_synonym="lm; znT-4; Znt4"
ORIGIN      
        1 agtggctggg gctcggcctc ggtccctggc ccgggtcgca gtagcgcgag ctccgggcgg
       61 ggatgcgcgg cggcgtcggc ccgcaggtag ccgagcaccg ccgagtccgg agctggcgcg
      121 cggccggggc gcggcgacag gagcggggtc tcgttctcct cacgatgggc agggacgcgc
      181 cgcgctgccc tgaggagccc gagcgccggt gaaggcggag tcgccggccg cccctccgct
      241 gccatggccg gccccggcgc gtggaagcgc ctcaaatccc tgctgaggaa agacgacacg
      301 ccgctgttct taaatgacac cagcgccttc gacttctcgg acgaggtgag cgacgagggg
      361 ctttcgcgct ttaataaact tcgagtcgtg gtggccgacg atgactcgga agccccggaa
      421 aggcctgtga acggggcgca cccggccctc caggccgacg atgactcctt attggaccag
      481 gacttacctt taaccaacag tcagctgagt ttgaaaatgg acccctgtga caactgcagc
      541 aagcgacgcg agttgctgaa gcagaggaag gtgaagacca ggctgaccat cgctgccgtc
      601 ctctacttgc ttttcatgat cggagagctt gtaggtggat acatggcaaa tagcttagca
      661 attatgacag atgcactcca tatgctaact gaccttagtg ccatcatact cactctgctt
      721 gctttgtggc tgtcctcaaa gtcaccaacc agaagattca cctttggatt tcatcgccta
      781 gaggttttgt cggccatgat cagtgtaatg ctggtgtatg tactaatggg attcctctta
      841 tatgaggctg tgcagagaac catccatatg aactatgaaa taaacggcga tgtcatgctc
      901 atcactgcag ctgttggagt tgcagttaat gtaataatgg ggtttctgtt gaaccagtcc
      961 ggtcaccacc attctcacgc tcattcccac tccctgcctt caaattcccc aagcatggta
     1021 tctagtggac acaaccatgg gcaggacagc ttggcagtga gagctgcatt tgtacacgcc
     1081 ttgggagact tggtacaaag tgttggtgtg cttatagctg catacatcat acgattcaag
     1141 ccagaataca agattgctga tcccatctgt acgtatatat tttcattgct tgtggctttc
     1201 acaacgtttc gcatcatatg ggacacagta gtgataatcc tggaaggtgt accaagccat
     1261 ttgaatgtag attatatcaa agagtcgttg atgaaaatag aagatgtata ttctgtggaa
     1321 gatttgaata tctggtctct cacttctgga aaatccactg ccatagttca catgcagcta
     1381 attcctggaa gttcatctaa atgggaagaa gtacagtcca aagctaagca tttattactg
     1441 aacacgtttg gcatgtacaa atgcactatt cagctccaga gttacagaca agaagtgatc
     1501 agaacttgtg caaattgtca cagttccagt acctgagttc caggtgtatg tcctcggggt
     1561 ctacggcctt gtttatcctg aagtacagac ttgagagcaa taaatgtaaa cctaatggag
     1621 tccctgacag ctgtgtccat gtcaagcacc agtctcccca agacagactc cgctctgact
     1681 aatatttgca tgatttcaga caaagaactt ccagagtgct gtttacagaa tgagctgtca
     1741 ccctgcagat acctcatcag aagacaatcc aagaccatgc ttcattaatt tgacagagta
     1801 tgcaacatgt acacgaagac tcagaattca gtatttgcat ctaacaacga cttttttcag
     1861 gccttttata tgaagtcagt gctagcaaac tcagtgttga actatttggg atttttgtct
     1921 ctgtgtaatc ttggcatcca gtttctggaa ttgtgttctc tttttataaa aattagccct
     1981 caacagattt cagaaagtaa ttatatccag aaaatggaat aagcatgtat tctaagtgtt
     2041 tcagaactgt tttatttcac atataactta taagcaaatt ttccagatag tactgggttt
     2101 taaatctcaa tatttttgat tatttgtaat cttgtcagaa atattcacat aatgtggtca
     2161 ctgtccttaa gttatttagg gaaactttaa tataattata aatggcagaa attagattta
     2221 acctcaaatt accttaccat atgatgatct aaagcagaga agcaagtagt ggtctgtaga
     2281 gttttggatt ctatcttcca gaaaatcact tctgtgcttt atgtagaatt gtcttaggac
     2341 ggtaagagac aacaacattt cagcagttat tcactgtcct ttttagaact gccaacattt
     2401 gttagtaata gtttgcatag caccctctca tgtgcacacc aggttatcta tacatctttc
     2461 tgagtaccta tagaagctaa ggctgaggtt attaatgccc accatgtaga gggcctttgt
     2521 tactccagag aaaatttctg ttatgataca ggctgttctc catgggttgc agaactacaa
     2581 gttttacagt aatgtcataa tagaaagata cagaggcatt gggtgttctc tttaagaaaa
     2641 cacaaggtaa aggttttgtt tgttttgtct tgtcttgttt tgttttgttt tgttttgttt
     2701 tgttttgttt ggccatgtat gtgcacagaa gaactgaaat aaggatggac actaaacctg
     2761 tgtctgagga agcaacataa atatttccaa gctcaaggga aggcaattgg cagcagttgt
     2821 gctaaagaga aagtcatgaa tattcacgat tcattcccag cagttgcttt gagatgagct
     2881 catttcaacc tagcactttg tatggctctt gtctcctcaa agagtcagag tggagtaact
     2941 ctgtaccaaa cagattgtga atagaaatcc aaatggtgtg taaatgaaga gtgggaagtt
     3001 gagactttcc ctgtgttaaa aagtaataag aaaacctggg tttacccaaa aggttgttgg
     3061 gaattgatag gttcaaaaca cagctgatgt ttctccataa aactcaatgg gtatttgttg
     3121 aattcaaagt ttactttgaa aaaaacaaaa agtttacttt gttgaatttt tattgtgagt
     3181 ccatttaaaa ttataaatac ttgaacttca aattttctta gaatataaac taaataaaat
     3241 agtgattcgc atatgagtag attataggca tttaagtggc tgcctgggca tttgtggcct
     3301 ttagatatgt ctaggggaaa aaaggaaaat gtgcatttta ttcttaacat tttcacacat
     3361 cagttgagct catttcttga aaacatcctt ttaccataat atgggcatat ctggattttc
     3421 atgttgatat ttggtaaaac agccttaaaa tccatttgaa tattgaaaaa gttttcataa
     3481 aaaaaaacag aagaacttaa aacttctgta agtcttaact atcatattca tgtaaagtgg
     3541 gtgtacctgt aatctctgca cttgcctttt gcagcagggg ctagacgttg ttcttcatgg
     3601 gtccacaaga tggagagctg gtagattttt acttgtttag cttgtcagaa cccatagagg
     3661 aaggacatta agtcagcagt tagatcaggg actacacagc tgtaagatct ccgtctttct
     3721 cctttggaga cacagtgagt ggcacaacct ctcagttaac tgtgagaact ctttgccctg
     3781 caaagaagca ggatctgaat tggggagatg cagtgctaat cgtgcactgg gaaacatcac
     3841 aacataaaac ttggcaattc tccactgcac tatattctaa ggagtatttt cagataatta
     3901 attactgtag acattaaccc atatttcaca acttgtgccc aatgcagtct tttgttttag
     3961 gaagactctg tctgactaac aatcttgctg tcttaagaag cataccaaga atgcagttga
     4021 gtaagacata cttaacaggc agaaccagaa gctattgtaa ttcttacggg cagctatgtt
     4081 aactttccac agctaaaggt tttcatgtga gcagattttc acattcacag cttccatcca
     4141 cattgtttac cagatcacta gatgtcttgg tttacagtgt ttcttttcag tttgggatag
     4201 aggttttcat ttttctctct gacattattt cctgaactct caacgttgtc ttaagcccag
     4261 gtggcccacg tgtcagtatt tgtcttcagg agagccttcc cacagtggac acagtcaaga
     4321 tcagagagaa acacgagcat cactctcagt tttcagagag aactttcagc caatacttag
     4381 aaatacattt gcaggtgaga aaggttttta aaacctcaga tcatcccctg tccagatttt
     4441 ctgcatggga acatggtaga gaatatgaga cagaaataga ctgagcagct actgtgaaga
     4501 gctttgagtt tctcaaaatg ccagccagca ttcttagaga ttttgatcct gaagtaactt
     4561 cagctgtgtt tggttttctg tattgatgac cactcgttca tcagcatacg atgttcttat
     4621 agaatctgaa ctctgctgtt gactaccttg tatgtagtca agataaagct acagaatgtg
     4681 ccactacgta tgataccatt tggttcagtt catcgttgga ccactcttag ttacaaataa
     4741 tcagtgacat ctcactagct ctactgtgct aagtaagcag ggagaaaatc tggtgtctag
     4801 gaaatggttt aatatctctg tagctagaac accatttgca aactcataat cccacctcca
     4861 ggtctgtcta atggacccta tgggcttaag atttgatttt gcagataggc atattgtttg
     4921 tgatgttagt gggagcatgc aaactagaga aaaaaaattg taggaattcc tcagtattat
     4981 gtgtttccat ttaggcaacc cgtcagtaag ttatggttac aaaagatttg ttggagacca
     5041 acagtcattt tctgattttt gtgaagattg gtgtttatgg agaccttgcc agctaaatcc
     5101 atgacagtgt ctatttaaat agtgcaatca agaataccct gaaataaatg caacccaaag
     5161 ttgaaatgtg ccaggatgcc ccttttctac ccaggagata tcttatattt ggaaaatgtc
     5221 tgcctctcca aataaatatg tgattaataa gccatatcac agtttaagaa attgtatata
     5281 tgcccctcca aaaaccaagt attttgcata tgattgattt tagaaagatt ttgaagctgg
     5341 ggtttatctg tgtaatgaag accagattct ttgtatatgt actgcatttg taattttcaa
     5401 atgaaatttc ctatacgatt attaaataaa gtgttgtttt gccttgtggt ttattaaaaa
     5461 cttaaaatgt acagtcccct aaaaaaaaaa aaaaaaa
//
