LOCUS       NM_001357771            2052 bp    mRNA    linear   ROD 12-OCT-2024
DEFINITION  Mus musculus solute carrier family 29 (nucleoside transporters),
            member 1 (Slc29a1), transcript variant 6, mRNA.
ACCESSION   NM_001357771 XM_006524771
VERSION     NM_001357771.2
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Chen,C., Xie,T., Zhang,Y., Wang,Y., Yu,F., Lin,L., Zhang,W.,
            Brown,B.C., Zhang,X., Kellems,R.E., D'Alessandro,A. and Xia,Y.
  TITLE     Erythrocyte ENT1-AMPD3 Axis is an Essential Purinergic Hypoxia
            Sensor and Energy Regulator Combating CKD in a Mouse Model
  JOURNAL   J Am Soc Nephrol 34 (10), 1647-1671 (2023)
   PUBMED   37725437
  REMARK    GeneRIF: Erythrocyte ENT1-AMPD3 Axis is an Essential Purinergic
            Hypoxia Sensor and Energy Regulator Combating CKD in a Mouse Model.
REFERENCE   3  (bases 1 to 2052)
  AUTHORS   Ruan,W., Li,J., Choi,S., Ma,X., Liang,Y., Nair,R., Yuan,X.,
            Mills,T.W. and Eltzschig,H.K.
  TITLE     Targeting myocardial equilibrative nucleoside transporter ENT1
            provides cardioprotection by enhancing myeloid Adora2b signaling
  JOURNAL   JCI Insight 8 (11), e166011 (2023)
   PUBMED   37288658
  REMARK    GeneRIF: Targeting myocardial equilibrative nucleoside transporter
            ENT1 provides cardioprotection by enhancing myeloid Adora2b
            signaling.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 2052)
  AUTHORS   Chen,K.Y., Lu,C.S., Pang,C.Y., Ho,C.J., Wu,K.C., Yang,H.W.,
            Lai,H.L., Chern,Y. and Lin,C.J.
  TITLE     Equilibrative Nucleoside Transporter 1 is a Target to Modulate
            Neuroinflammation and Improve Functional Recovery in Mice with
            Spinal Cord Injury
  JOURNAL   Mol Neurobiol 60 (1), 369-381 (2023)
   PUBMED   36269542
  REMARK    GeneRIF: Equilibrative Nucleoside Transporter 1 is a Target to
            Modulate Neuroinflammation and Improve Functional Recovery in Mice
            with Spinal Cord Injury.
REFERENCE   5  (bases 1 to 2052)
  AUTHORS   Niemann,B., Haufs-Brusberg,S., Puetz,L., Feickert,M.,
            Jaeckstein,M.Y., Hoffmann,A., Zurkovic,J., Heine,M.,
            Trautmann,E.M., Muller,C.E., Tonjes,A., Schlein,C., Jafari,A.,
            Eltzschig,H.K., Gnad,T., Bluher,M., Krahmer,N., Kovacs,P.,
            Heeren,J. and Pfeifer,A.
  TITLE     Apoptotic brown adipocytes enhance energy expenditure via
            extracellular inosine
  JOURNAL   Nature 609 (7926), 361-368 (2022)
   PUBMED   35790189
REFERENCE   6  (bases 1 to 2052)
  AUTHORS   Coe,I., Zhang,Y., McKenzie,T. and Naydenova,Z.
  TITLE     PKC regulation of the human equilibrative nucleoside transporter,
            hENT1
  JOURNAL   FEBS Lett 517 (1-3), 201-205 (2002)
   PUBMED   12062437
  REMARK    GeneRIF: PKC delta/epsilon-dependent stimulation of hENT1 transport
            by a mechanism that may involve activation of transporters at the
            membrane possibly by post-translational modification of the
            protein.
REFERENCE   7  (bases 1 to 2052)
  AUTHORS   Piao,Y., Ko,N.T., Lim,M.K. and Ko,M.S.
  TITLE     Construction of long-transcript enriched cDNA libraries from
            submicrogram amounts of total RNAs by a universal PCR amplification
            method
  JOURNAL   Genome Res 11 (9), 1553-1558 (2001)
   PUBMED   11544199
REFERENCE   8  (bases 1 to 2052)
  AUTHORS   Handa,M., Choi,D.S., Caldeiro,R.M., Messing,R.O., Gordon,A.S. and
            Diamond,I.
  TITLE     Cloning of a novel isoform of the mouse NBMPR-sensitive
            equilibrative nucleoside transporter (ENT1) lacking a putative
            phosphorylation site
  JOURNAL   Gene 262 (1-2), 301-307 (2001)
   PUBMED   11179696
REFERENCE   9  (bases 1 to 2052)
  AUTHORS   Kiss,A., Farah,K., Kim,J., Garriock,R.J., Drysdale,T.A. and
            Hammond,J.R.
  TITLE     Molecular cloning and functional characterization of
            inhibitor-sensitive (mENT1) and inhibitor-resistant (mENT2)
            equilibrative nucleoside transporters from mouse brain
  JOURNAL   Biochem J 352 Pt 2 (Pt 2), 363-372 (2000)
   PUBMED   11085929
REFERENCE   10 (bases 1 to 2052)
  AUTHORS   Choi,D.S., Handa,M., Young,H., Gordon,A.S., Diamond,I. and
            Messing,R.O.
  TITLE     Genomic organization and expression of the mouse equilibrative,
            nitrobenzylthioinosine-sensitive nucleoside transporter 1 (ENT1)
            gene
  JOURNAL   Biochem Biophys Res Commun 277 (1), 200-208 (2000)
   PUBMED   11027664
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC163677.2.
            
            On Dec 6, 2019 this sequence version replaced NM_001357771.1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7652917.1226656.1,
                                           SRR5189685.177001.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN01164143 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: inferred from homology
            RefSeq Select criteria             :: based on conservation,
                                                  expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-49                AC163677.2         8969-9017
            50-150              AC163677.2         11067-11167
            151-233             AC163677.2         12238-12320
            234-315             AC163677.2         14161-14242
            316-518             AC163677.2         14374-14576
            519-658             AC163677.2         14679-14818
            659-793             AC163677.2         14951-15085
            794-891             AC163677.2         15194-15291
            892-964             AC163677.2         15425-15497
            965-1062            AC163677.2         16113-16210
            1063-1171           AC163677.2         16829-16937
            1172-1257           AC163677.2         17186-17271
            1258-1469           AC163677.2         18194-18405
            1470-2052           AC163677.2         18717-19299
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="17"
                     /map="17 22.59 cM"
     gene            1..2052
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /note="solute carrier family 29 (nucleoside transporters),
                     member 1"
                     /db_xref="GeneID:63959"
                     /db_xref="MGI:MGI:1927073"
     exon            1..49
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            50..150
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            151..233
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     CDS             205..1581
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /note="isoform 2 is encoded by transcript variant 6;
                     nucleoside transporter, es-type; solute carrier family 29
                     member 1; equilibrative NBMPR-sensitive nucleoside
                     transporter; equilibrative nitrobenzylmercaptopurine
                     riboside-sensitive nucleoside transporter; NBMPR-sensitive
                     equilibrative nucleoside transporter"
                     /codon_start=1
                     /product="equilibrative nucleoside transporter 1 isoform
                     2"
                     /protein_id="NP_001344700.1"
                     /db_xref="CCDS:CCDS28813.1"
                     /db_xref="GeneID:63959"
                     /db_xref="MGI:MGI:1927073"
                     /translation="MTTSHQPQDRYKAVWLIFFVLGLGTLLPWNFFMTATKYFTNRLD
                     VSQNVSSDTDQSCESTKALADPTVALPARSSLSAIFNNVMTLCAMLPLLVFTCLNSFL
                     HQRISQSVRILGSLLAILLVFLVTAALVKVEMDALIFFVITMIKIVLINSFGAILQAS
                     LFGLAGVLPANYTAPIMSGQGLAGFFTSVAMICAIASGSELSESAFGYFITACAVVIL
                     AILCYLALPRTEFYRHYLQLNLAGPAEQETKLDLIREEPKGRREESGVPGPNSPPTNR
                     NQSIKAILKSICVPALSVCFIFTVTIGLFPAVTAEVESSIAGTSPWKSYFIPVACFLN
                     FNVFDWLGRSLTAVCMWPGQDSRWLPVLVASRIVFIPLLMLCNVKARHCGAQRHHFVF
                     KHDAWFIAFMAAFAFSNGYLASLCMCFGPKKVKPAEAETAGNIMSFFLCLGLALGAVL
                     SFLLRALV"
     exon            234..315
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            316..518
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            519..658
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            659..793
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            794..891
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            892..964
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            965..1062
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1063..1171
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1172..1257
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1258..1469
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     exon            1470..2052
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2030..2035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /note="hexamer: AATAAA"
     polyA_site      2052
                     /gene="Slc29a1"
                     /gene_synonym="1200014D21Rik; ENT1; mENT1"
                     /note="major polyA site"
ORIGIN      
        1 gctggccaag cgcgcagatt tcgtcgccgg aggtgtcctt ctccagcagg acccaacctc
       61 ctgcctgagc ctgagccagg accccttcct ggggaacgcc caggtacctt tggctctcac
      121 cgtcctgcca acatcgagtc tataaatttg caagagccag agggagggag ctgccagcca
      181 ggcacagccg agaacactgg agccatgaca accagtcacc agcctcagga caggtataag
      241 gcagtatggc ttatcttctt tgtgctgggc ctggggacac tgctcccctg gaattttttt
      301 atgaccgcaa ccaagtattt cacaaaccgc ctggacgtgt cccagaatgt gtcctcggac
      361 actgatcaat catgcgaaag caccaaggcc ttggctgacc ccacagtggc cttgccagcc
      421 cggagttctc tcagtgccat cttcaacaat gtcatgaccc tgtgtgccat gctgcccttg
      481 ctggtcttca cctgcctcaa ctcgtttctg catcagcgga tctctcaatc tgttcggatc
      541 ttgggcagcc tgctggcaat cctgctggta ttccttgtca ctgccgccct ggtgaaggtg
      601 gagatggatg ctctgatctt ctttgtcatc accatgatca agattgtgct catcaattca
      661 tttggtgcca ttttgcaagc cagccttttt ggtctggcag gtgtcctgcc agccaactac
      721 acagccccca tcatgagtgg ccagggcctg gctggcttct tcacctctgt cgccatgatc
      781 tgtgccattg ccagtggttc tgagctgtca gaaagcgcct ttggctactt catcacagcc
      841 tgtgcagttg tcattttggc catcctgtgc tacctggctc tgcctcggac ggaattctat
      901 cgccattacc tgcagctcaa ccttgcgggg cctgcagagc aggagaccaa gttggatctc
      961 ataagagagg agccaaaagg aagaagagag gaatctgggg tgccaggccc caactctcca
     1021 cccaccaaca gaaaccagtc tatcaaagcc atacttaaga gtatctgtgt cccggctctg
     1081 tctgtctgct tcatcttcac ggttaccatt gggttgttcc ctgctgtgac tgctgaggtg
     1141 gaatccagca tcgcaggcac aagtccctgg aaaagctact tcattcccgt ggcctgtttc
     1201 ttgaatttca atgtctttga ctggctaggc cggagcctca ctgctgtctg catgtggcct
     1261 ggccaggata gccgctggct gccggttttg gtcgcctcga ggattgtgtt tattcccctg
     1321 ctgatgctct gcaacgtgaa ggctcgccac tgcggcgcgc agcggcacca cttcgtcttt
     1381 aagcatgacg cctggttcat cgccttcatg gctgcctttg ccttctccaa tggctacctc
     1441 gccagcctct gcatgtgctt cgggcccaag aaagtcaaac cagctgaggc ggagacagca
     1501 ggaaacatca tgtccttctt tctgtgtctg ggcctggctc tgggagctgt gttgtccttc
     1561 ttgttaaggg cacttgtgtg accctgtggg gacagaagaa ctacactgcc tgcttcctgc
     1621 tcacttcctt ccctgccagg gacgagcagg ggtcgagagg ggctgttctt ctagctgact
     1681 tctgctttcc tctggactgt gcttcgccca gctgtccagg agccagcgat ggcctgcggg
     1741 tggacttgga attcagggtc agaatggcaa gggctcaatg gcctctgact gacagctccg
     1801 actgatgccc gcttactcca agcacaagag actccagggc caagagagat ctgtccgcct
     1861 gcctatcaca ggatagggcg gaggcggatg gctgattggt gtcgtgtgac ctgatgtccc
     1921 tccccttgcc cttcttcctt ctgtgcctgt tccatgtccc cagcccttgt cattttactg
     1981 ccttttttat actgacagaa accaggtgcc ttcagaggcc atctgattaa ataaacattt
     2041 tttttctcca ta
//
