LOCUS       NM_172980               3335 bp    mRNA    linear   ROD 01-JUN-2024
DEFINITION  Mus musculus solute carrier family 28 (sodium-coupled nucleoside
            transporter), member 2 (Slc28a2), transcript variant 1, mRNA.
ACCESSION   NM_172980 NM_021520 XM_030251659 XM_988760 XM_988795
VERSION     NM_172980.4
KEYWORDS    RefSeq; RefSeq Select.
SOURCE      Mus musculus (house mouse)
  ORGANISM  Mus musculus
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha;
            Muroidea; Muridae; Murinae; Mus; Mus.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Adams,D.J., Barlas,B., McIntyre,R.E., Salguero,I., van der
            Weyden,L., Barros,A., Vicente,J.R., Karimpour,N., Haider,A.,
            Ranzani,M., Turner,G., Thompson,N.A., Harle,V., Olvera-Leon,R.,
            Robles-Espinoza,C.D., Speak,A.O., Geisler,N., Weninger,W.J.,
            Geyer,S.H., Hewinson,J., Karp,N.A., Fu,B., Yang,F., Kozik,Z.,
            Choudhary,J., Yu,L., van Ruiten,M.S., Rowland,B.D., Lelliott,C.J.,
            Del Castillo Velasco-Herrera,M., Verstraten,R., Bruckner,L.,
            Henssen,A.G., Rooimans,M.A., de Lange,J., Mohun,T.J., Arends,M.J.,
            Kentistou,K.A., Coelho,P.A., Zhao,Y., Zecchini,H., Perry,J.R.B.,
            Jackson,S.P. and Balmus,G.
  CONSRTM   Sanger Mouse Genetics Project
  TITLE     Genetic determinants of micronucleus formation in vivo
  JOURNAL   Nature 627 (8002), 130-136 (2024)
   PUBMED   38355793
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Jaballah,N., Tsurudome,Y., Murakami,C., Matsunaga,N., Ushijima,K.,
            Koyanagi,S. and Ohdo,S.
  TITLE     The scaffold protein PDZK1 governs diurnal localization of CNT2 on
            the plasma membrane in mouse intestinal epithelial cells
  JOURNAL   J Biochem 174 (2), 193-201 (2023)
   PUBMED   37159505
  REMARK    GeneRIF: The scaffold protein PDZK1 governs diurnal localization of
            CNT2 on the plasma membrane in mouse intestinal epithelial cells.
REFERENCE   3  (bases 1 to 3335)
  AUTHORS   Yong,T., Chen,S., Xie,Y., Chen,D., Su,J., Shuai,O., Hu,H., Zuo,D.
            and Liang,D.
  TITLE     Hypouricemic Effects of Armillaria mellea on Hyperuricemic Mice
            Regulated through OAT1 and CNT2
  JOURNAL   Am J Chin Med 46 (3), 585-599 (2018)
   PUBMED   29595077
  REMARK    GeneRIF: It is a purine nucleoside preferring transporter playing a
            central role in the gastrointestinal absorption of purines in the
            enterocytes.
REFERENCE   4  (bases 1 to 3335)
  AUTHORS   Errasti-Murugarren,E., Fernandez-Calotti,P., Veyhl-Wichmann,M.,
            Diepold,M., Pinilla-Macua,I., Perez-Torras,S., Kipp,H., Koepsell,H.
            and Pastor-Anglada,M.
  TITLE     Role of the transporter regulator protein (RS1) in the modulation
            of concentrative nucleoside transporters (CNTs) in epithelia
  JOURNAL   Mol Pharmacol 82 (1), 59-67 (2012)
   PUBMED   22492015
REFERENCE   5  (bases 1 to 3335)
  AUTHORS   Furihata,T., Fukuchi,Y., Iikura,M., Hashizume,M., Miyajima,A.,
            Nagai,M. and Chiba,K.
  TITLE     Striking species difference in the contribution of concentrative
            nucleoside transporter 2 to nucleoside uptake between mouse and rat
            hepatocytes
  JOURNAL   Antimicrob Agents Chemother 54 (7), 3035-3038 (2010)
   PUBMED   20421393
  REMARK    GeneRIF: Data concluded that CNT2 contributes considerably to
            nucleoside uptake in rat hepatocytes but not in mouse hepatocytes.
REFERENCE   6  (bases 1 to 3335)
  AUTHORS   Valdes,R., Fernandez-Veledo,S., Aymerich,I., Casado,F.J. and
            Pastor-Anglada,M.
  TITLE     TGF-beta transcriptionally activates the gene encoding the
            high-affinity adenosine transporter CNT2 in rat liver parenchymal
            cells
  JOURNAL   Cell Mol Life Sci 63 (21), 2527-2537 (2006)
   PUBMED   17013559
  REMARK    GeneRIF: TGF-beta1 transcriptionally activates the CNT2-encoding
            gene in a JNK-dependent manner.
REFERENCE   7  (bases 1 to 3335)
  AUTHORS   Nagai,K., Nagasawa,K., Koma,M., Hotta,A. and Fujimoto,S.
  TITLE     Cytidine is a novel substrate for wild-type concentrative
            nucleoside transporter 2
  JOURNAL   Biochem Biophys Res Commun 347 (2), 439-443 (2006)
   PUBMED   16828706
REFERENCE   8  (bases 1 to 3335)
  AUTHORS   Sandell,L.L., Guan,X.J., Ingram,R. and Tilghman,S.M.
  TITLE     Gatm, a creatine synthesis enzyme, is imprinted in mouse placenta
  JOURNAL   Proc Natl Acad Sci U S A 100 (8), 4622-4627 (2003)
   PUBMED   12671064
REFERENCE   9  (bases 1 to 3335)
  AUTHORS   Patel,D.H., Crawford,C.R., Naeve,C.W. and Belt,J.A.
  TITLE     Cloning, genomic organization and chromosomal localization of the
            gene encoding the murine sodium-dependent, purine-selective,
            concentrative nucleoside transporter (CNT2)
  JOURNAL   Gene 242 (1-2), 51-58 (2000)
   PUBMED   10721696
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL844566.8.
            
            On or before Apr 3, 2020 this sequence version replaced
            XM_030251659.1, NM_172980.3.
            
            Transcript Variant: This variant (1) represents the longest
            transcript. Variants 1-4 all encode the same protein.
            
            ##Evidence-Data-START##
            CDS exon combination :: AF079853.2 [ECO:0000331]
            RNAseq introns       :: single sample supports all introns
                                    SAMN00849375, SAMN00849377 [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AL844566.8         57369-57569         c
            202-553             AL844566.8         43799-44150         c
            554-740             AL844566.8         42998-43184         c
            741-837             AL844566.8         42349-42445         c
            838-929             AL844566.8         42153-42244         c
            930-1024            AL844566.8         37024-37118         c
            1025-1208           AL844566.8         36051-36234         c
            1209-1350           AL844566.8         34100-34241         c
            1351-1464           AL844566.8         32965-33078         c
            1465-1542           AL844566.8         32399-32476         c
            1543-1623           AL844566.8         31941-32021         c
            1624-1704           AL844566.8         31440-31520         c
            1705-1830           AL844566.8         29398-29523         c
            1831-1961           AL844566.8         29185-29315         c
            1962-2130           AL844566.8         28604-28772         c
            2131-2328           AL844566.8         28248-28445         c
            2329-2410           AL844566.8         27602-27683         c
            2411-2509           AL844566.8         26022-26120         c
            2510-2621           AL844566.8         25725-25836         c
            2622-3335           AL844566.8         22871-23584         c
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Mus musculus"
                     /mol_type="mRNA"
                     /strain="C57BL/6"
                     /db_xref="taxon:10090"
                     /chromosome="2"
                     /map="2 60.63 cM"
     gene            1..3335
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /note="solute carrier family 28 (sodium-coupled nucleoside
                     transporter), member 2"
                     /db_xref="GeneID:269346"
                     /db_xref="MGI:MGI:1913105"
     exon            1..201
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            202..553
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            554..740
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            741..837
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     CDS             757..2739
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /note="purine-selective Na+ nucleoside cotransporter;
                     concentrative nucleoside transporter 2; sodium/purine
                     nucleoside cotransporter; Na(+)/nucleoside cotransporter
                     2"
                     /codon_start=1
                     /product="sodium/nucleoside cotransporter 2"
                     /protein_id="NP_766568.1"
                     /db_xref="CCDS:CCDS16663.1"
                     /db_xref="GeneID:269346"
                     /db_xref="MGI:MGI:1913105"
                     /translation="MEKSKGRKSVSQATVENCMENPGLELMEGGNLEQRYTQEEVTQG
                     HSLEDGLGHSSLWSRRIFQPFTKARSFFERHAGLFRKILLGLLCLAYAAYFLAACILN
                     FQRALALFVITCLVIFILACHFLKKFFPKEQLRCLKPLENTHLNLWAKRVFVGLSVVG
                     LILWLALDTAQRPEQLISFAGICMFILILFACSKHHSAVCWRTVFWGLGLQFIFGILV
                     IRTEPGFNAFQWLGDQIQIFLAYTVEGSSFVFGDTLVQNVFAFQSLPIIIFFGCVMSI
                     LYYLGLVQWVIQKVAWFLQITMGTTAAETLAVAGNIFVGMTEAPLLIRPYLADMTISE
                     IHAVMTGGFATIAGTVLGAFISFGIDASSLISASVMAAPCALALSKLVYPEVEESKFK
                     SKEGLKLPRGEERNILEAASNGATDAISLVANVAANLIAFLAVLAFINATLSWLGEMV
                     DIHGLSFQVICSYVLRPMVFMMGVQWADCPLVAEIVGVKFFINEFVAYQQLSQYKNKR
                     LSGVEEWINGEKQWISVKAEIITTFSLCGFANLSSIGITLGGLTSMIPQRKSDLCKIV
                     VRALFTGACVSFISACMAGILYVPRGAETDCVSFLNTNFTNRTYETYVCCRELFQSTS
                     LNGTNMPSFSGPWQDNVSSLRNLASCCDLYTSTVCA"
     exon            838..929
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            930..1024
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            1025..1208
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1350
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            1351..1464
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            1465..1542
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            1543..1623
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            1624..1704
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            1705..1830
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            1831..1961
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            1962..2130
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2328
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            2329..2410
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            2411..2509
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            2510..2621
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..3335
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3316..3321
                     /regulatory_class="polyA_signal_sequence"
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /note="hexamer: AATAAA"
     polyA_site      3335
                     /gene="Slc28a2"
                     /gene_synonym="2010208B10Rik; B430217P18; Cnt2; SPNT"
                     /note="major polyA site"
ORIGIN      
        1 aatccttgct gcagctgtgc atcctcgctg acatcagact ccagtgtctc cgggatccag
       61 tggacattga agatcggtag ctctccagga gtcttcaaag caccaggttt ggaagtgctg
      121 aggcttctgg ctttgtaacc tgaacagctc ccaggttcca gccattgttg aactgggctg
      181 tgtggaccca tccagcacac tgcttcacag gtccaggatg gagatgtgga aatggaaatg
      241 gcacctcatc agcatcgcca ttgagccacc agccaaactt ttgctttatg ttccacaagc
      301 ttatgctctg ttggcctaga agttttgacc ccagaagtgg gggtgctttg gtcaggagac
      361 atcccactga agtggaagct aagactactc tctagccaca ttggaagcaa caatagagtt
      421 aggaggggtg gttgacccag accaaaggga aattggactc tttccccaca gtggaggtaa
      481 ggaagatcat atcaaatgca agagatcggc actgtgacta aagccaatgg gaaactacaa
      541 caactcaacc cagctgtgca tcctcactga catcagactc cagtgtctcc gggatccagt
      601 ggacattgaa gatcggtagc tctccaggag tcttcaaagc accaggtttg gaagtgctga
      661 ggcttctggc tttgtaacct gaacagctcc caggttccag ccattgttgg actgggctgt
      721 gtggacccat ccagcacact caggtgataa caagatatgg agaagtcaaa gggaagaaag
      781 tctgtttccc aggccacagt ggagaattgc atggagaacc caggcctgga actcatggaa
      841 ggcggaaatc ttgagcaaag atacacacag gaggaagtga cacagggaca cagcctggag
      901 gatggcttgg ggcattcctc tctctggagc aggaggattt ttcagccttt cacgaaggcg
      961 agaagtttct ttgaaagaca tgctggttta ttcagaaaga tcctgcttgg tctcttgtgt
     1021 ttagcttatg ctgcctactt cctggcagcg tgcatcctga acttccagag ggcactggcc
     1081 ttgtttgtca tcacctgtct tgtgatcttc atcctggctt gccattttct gaaaaaattc
     1141 tttcctaaag aacaactaag atgcttgaag cctctggaaa acacccacct gaatctttgg
     1201 gcaaagcggg tgttcgtagg actctctgtt gttggcctca tcctctggct ggctctcgac
     1261 acagctcaaa gaccagagca gctgatctcc tttgcaggaa tctgtatgtt catcctcatc
     1321 ctctttgcct gctccaaaca ccacagtgcg gtgtgctgga ggacggtgtt ttggggcctg
     1381 ggccttcagt tcatctttgg aattttggtc atcagaactg aacctggatt taatgcattt
     1441 caatggctgg gagatcagat ccagattttc ttggcttata ctgtggaagg atccagtttt
     1501 gtttttggtg atacactggt ccaaaatgtt tttgcttttc agtctctgcc aatcattatt
     1561 ttcttcggat gtgtgatgtc tattctttac tacctgggcc ttgtgcagtg ggtgattcag
     1621 aaggttgcct ggttcctgca aattaccatg ggtaccactg ctgcagagac cctggccgtg
     1681 gcaggaaaca tctttgtggg gatgacagag gcgcctctgc tcatccgtcc ctaccttgca
     1741 gacatgacca tctctgaaat ccatgcagtc atgactggag gctttgctac catagcaggc
     1801 acagtgttgg gagccttcat atcctttggg attgatgcat catccttgat ttctgcctca
     1861 gtgatggctg ccccttgtgc acttgccttg tccaaactgg tatatccaga agtcgaagag
     1921 tccaagttca agagcaagga gggactgaag ctgcctcgag gggaagagag gaatatcctg
     1981 gaagctgcaa gcaatggagc cacagatgcc ataagccttg ttgctaatgt tgcagccaac
     2041 ctgattgcct ttctggctgt attggccttc atcaatgcta ccctctcttg gctgggggaa
     2101 atggtggata tccatggact cagtttccag gtcatctgct cctatgtcct aaggcccatg
     2161 gttttcatga tgggtgtaca gtgggcagac tgcccactgg tggctgagat agtgggagtc
     2221 aagttcttca taaatgaatt tgtggcctac cagcaactgt ctcagtacaa gaacaaacgt
     2281 ctctctggag tagaagagtg gatcaatggc gagaaacagt ggatttctgt gaaagctgaa
     2341 atcattacaa cattttccct ctgtggattt gccaatctta gttccatagg aatcacactg
     2401 ggaggcttga catccatgat accccagagg aagagtgact tgtgcaagat tgtggtcagg
     2461 gccctcttca caggtgcctg tgtttccttt atcagtgcct gcatggcagg aatcctctat
     2521 gttcccagag gcgctgaaac tgactgtgtc tcattcctaa acacaaattt caccaataga
     2581 acctatgaga catatgtgtg ctgcagagag cttttccaga gtacttcgct gaatggcacc
     2641 aacatgcctt ctttctcagg cccctggcaa gacaatgtgt ccagcctcag gaatcttgct
     2701 agctgctgtg acctctatac cagcactgtg tgtgcatgag gctggtgggc cgctccaaga
     2761 cagttctcat agaacagatt catattacca aatacatttt catgtacttc ctattctctg
     2821 agaaccccac taaactccac aaagtgaaat ttctgagctc actatagctc tgtataaaaa
     2881 tcagccctta cctttccagc ggcatgacat gaaattccgg ttaccagaca tgaaattcat
     2941 tatagaccct cttgagccaa agtatggtgg gcatgtaact gaggagaaaa tatgtgtgtg
     3001 gtgagggcta agatccaggt tcaaccccgg gcctggccca gagtggggag agtggggaaa
     3061 tcaaagtaga tgcttagttt taacttggga atggtggcaa accccgtggt ctcttacctg
     3121 taggctcaca gctaagggga ttacctcaga ttcaaggcaa gcctggccca aacatgacat
     3181 gtcctagtgt aggagtggat gtgaggggag atatttttaa agttatattg aaacatgtgt
     3241 gaatttaaag acacacattc tttttgtatt gccaaagact tataaacaca agagcttctt
     3301 ttattttcat aaagaaataa aaatgattcc tgtaa
//
